Effect of Factors Produced by the Placenta on Cytokine Secretion by THP-1 Cells Cultured on a 3D Scaffold
We compared the effects of soluble products from the placenta obtained from women with normal pregnancy and pregnancy complicated by preeclampsia on cytokine secretion by THP-1 cells cultured on a 3D Matrigel scaffold. In the presence of soluble products from all placentas, the cells actively secret...
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| Veröffentlicht in: | Bulletin of experimental biology and medicine 2014-02, Vol.156 (4), p.566-570 |
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| creator | Onokhina, Ya. S. L’vova, T. Yu Tsitskarava, D. Z. Koren’kov, D. A. Sel’kov, S. A. Sokolov, D. I. |
| description | We compared the effects of soluble products from the placenta obtained from women with normal pregnancy and pregnancy complicated by preeclampsia on cytokine secretion by THP-1 cells cultured on a 3D Matrigel scaffold. In the presence of soluble products from all placentas, the cells actively secreted IL-8, MCP-1, and soluble forms of CD14, TNFRI, and TNFRII receptors. Secretion of VEGF was below the spontaneous level. Secretion of IL-6 by THP-1 cells after incubation with soluble products of the placentas obtained during weeks 9-11 of physiological pregnancy and 38-39 of pregnancy complicated by preeclampsia surpassed the spontaneous level. In the presence of soluble factors of trimester I placentas, secretion of IL-6 and soluble form of TNFRI receptor was higher than in the presence of trimester III placental factors. Secretion of IL-6 by THP-1 cells was higher, while secretion of soluble TNFRII receptor was lower in the presence of placentas from women with preeclampsia in comparison with physiological pregnancy. |
| doi_str_mv | 10.1007/s10517-014-2397-0 |
| format | Article |
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S. ; L’vova, T. Yu ; Tsitskarava, D. Z. ; Koren’kov, D. A. ; Sel’kov, S. A. ; Sokolov, D. I.</creator><creatorcontrib>Onokhina, Ya. S. ; L’vova, T. Yu ; Tsitskarava, D. Z. ; Koren’kov, D. A. ; Sel’kov, S. A. ; Sokolov, D. I.</creatorcontrib><description>We compared the effects of soluble products from the placenta obtained from women with normal pregnancy and pregnancy complicated by preeclampsia on cytokine secretion by THP-1 cells cultured on a 3D Matrigel scaffold. In the presence of soluble products from all placentas, the cells actively secreted IL-8, MCP-1, and soluble forms of CD14, TNFRI, and TNFRII receptors. Secretion of VEGF was below the spontaneous level. Secretion of IL-6 by THP-1 cells after incubation with soluble products of the placentas obtained during weeks 9-11 of physiological pregnancy and 38-39 of pregnancy complicated by preeclampsia surpassed the spontaneous level. In the presence of soluble factors of trimester I placentas, secretion of IL-6 and soluble form of TNFRI receptor was higher than in the presence of trimester III placental factors. Secretion of IL-6 by THP-1 cells was higher, while secretion of soluble TNFRII receptor was lower in the presence of placentas from women with preeclampsia in comparison with physiological pregnancy.</description><identifier>ISSN: 0007-4888</identifier><identifier>EISSN: 1573-8221</identifier><identifier>DOI: 10.1007/s10517-014-2397-0</identifier><identifier>PMID: 24771450</identifier><identifier>CODEN: BEXBAN</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Biomedical and Life Sciences ; Biomedicine ; Cell Biology ; Cell Culture Techniques ; Cell Line ; Cytokines ; Cytokines - metabolism ; Cytokines - secretion ; Female ; Humans ; Internal Medicine ; Laboratory Medicine ; Pathology ; Physiological aspects ; Placenta - physiology ; Pre-Eclampsia - immunology ; Pre-Eclampsia - metabolism ; Pregnancy ; Tissue Extracts - physiology ; Vascular endothelial growth factor</subject><ispartof>Bulletin of experimental biology and medicine, 2014-02, Vol.156 (4), p.566-570</ispartof><rights>Springer Science+Business Media New York 2014</rights><rights>COPYRIGHT 2014 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c503t-4b64374a3ce2f8c8c082efb998ed35e8414d03a640f3cd083e8f959ac12bfd243</citedby><cites>FETCH-LOGICAL-c503t-4b64374a3ce2f8c8c082efb998ed35e8414d03a640f3cd083e8f959ac12bfd243</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10517-014-2397-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10517-014-2397-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24771450$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Onokhina, Ya. S.</creatorcontrib><creatorcontrib>L’vova, T. Yu</creatorcontrib><creatorcontrib>Tsitskarava, D. Z.</creatorcontrib><creatorcontrib>Koren’kov, D. A.</creatorcontrib><creatorcontrib>Sel’kov, S. A.</creatorcontrib><creatorcontrib>Sokolov, D. I.</creatorcontrib><title>Effect of Factors Produced by the Placenta on Cytokine Secretion by THP-1 Cells Cultured on a 3D Scaffold</title><title>Bulletin of experimental biology and medicine</title><addtitle>Bull Exp Biol Med</addtitle><addtitle>Bull Exp Biol Med</addtitle><description>We compared the effects of soluble products from the placenta obtained from women with normal pregnancy and pregnancy complicated by preeclampsia on cytokine secretion by THP-1 cells cultured on a 3D Matrigel scaffold. In the presence of soluble products from all placentas, the cells actively secreted IL-8, MCP-1, and soluble forms of CD14, TNFRI, and TNFRII receptors. Secretion of VEGF was below the spontaneous level. Secretion of IL-6 by THP-1 cells after incubation with soluble products of the placentas obtained during weeks 9-11 of physiological pregnancy and 38-39 of pregnancy complicated by preeclampsia surpassed the spontaneous level. In the presence of soluble factors of trimester I placentas, secretion of IL-6 and soluble form of TNFRI receptor was higher than in the presence of trimester III placental factors. Secretion of IL-6 by THP-1 cells was higher, while secretion of soluble TNFRII receptor was lower in the presence of placentas from women with preeclampsia in comparison with physiological pregnancy.</description><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Biology</subject><subject>Cell Culture Techniques</subject><subject>Cell Line</subject><subject>Cytokines</subject><subject>Cytokines - metabolism</subject><subject>Cytokines - secretion</subject><subject>Female</subject><subject>Humans</subject><subject>Internal Medicine</subject><subject>Laboratory Medicine</subject><subject>Pathology</subject><subject>Physiological aspects</subject><subject>Placenta - physiology</subject><subject>Pre-Eclampsia - immunology</subject><subject>Pre-Eclampsia - metabolism</subject><subject>Pregnancy</subject><subject>Tissue Extracts - physiology</subject><subject>Vascular endothelial growth factor</subject><issn>0007-4888</issn><issn>1573-8221</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkl9rFDEUxYModq1-AF8kIIgvU_N3k3ksY2uFggutzyGT3HSnzk5qknnYb2-GrdqKInlIcu_vHnLCQeg1JSeUEPUhUyKpaggVDeNtPTxBKyoVbzRj9ClakQo1Qmt9hF7kfLtcyZo-R0dMKEWFJCs0nIUAruAY8Ll1JaaMNyn62YHH_R6XLeDNaB1MxeI44W5f4rdhAnwFLkEZaqlS1xebhuIOxjHjbh7LnOp0bVnMP-IrZ0OIo3-JngU7Znh1vx-jr-dn191Fc_nl0-fu9LJxkvDSiH4tuBKWO2BBO-2IZhD6ttXguQQtqPCE27UggTtPNAcdWtlaR1kfPBP8GL0_6N6l-H2GXMxuyK6-zU4Q52yo0pJxqqX-PyppqwVhilT07R_obZzTVI1UQcUZZ1S2v6kbO4IZphBLsm4RNadcEUaZFLxSJ3-h6vKwG1ycIAy1_mjg3YOBLdixbHMc5-X_82OQHkCXYs4JgrlLw86mvaHELJExh8iYGhmzRMYszt7cO5v7HfhfEz8zUgF2AHJtTTeQHlj_p-oPzEnGBg</recordid><startdate>20140201</startdate><enddate>20140201</enddate><creator>Onokhina, Ya. 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S.</au><au>L’vova, T. Yu</au><au>Tsitskarava, D. Z.</au><au>Koren’kov, D. A.</au><au>Sel’kov, S. A.</au><au>Sokolov, D. I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Factors Produced by the Placenta on Cytokine Secretion by THP-1 Cells Cultured on a 3D Scaffold</atitle><jtitle>Bulletin of experimental biology and medicine</jtitle><stitle>Bull Exp Biol Med</stitle><addtitle>Bull Exp Biol Med</addtitle><date>2014-02-01</date><risdate>2014</risdate><volume>156</volume><issue>4</issue><spage>566</spage><epage>570</epage><pages>566-570</pages><issn>0007-4888</issn><eissn>1573-8221</eissn><coden>BEXBAN</coden><abstract>We compared the effects of soluble products from the placenta obtained from women with normal pregnancy and pregnancy complicated by preeclampsia on cytokine secretion by THP-1 cells cultured on a 3D Matrigel scaffold. In the presence of soluble products from all placentas, the cells actively secreted IL-8, MCP-1, and soluble forms of CD14, TNFRI, and TNFRII receptors. Secretion of VEGF was below the spontaneous level. Secretion of IL-6 by THP-1 cells after incubation with soluble products of the placentas obtained during weeks 9-11 of physiological pregnancy and 38-39 of pregnancy complicated by preeclampsia surpassed the spontaneous level. In the presence of soluble factors of trimester I placentas, secretion of IL-6 and soluble form of TNFRI receptor was higher than in the presence of trimester III placental factors. Secretion of IL-6 by THP-1 cells was higher, while secretion of soluble TNFRII receptor was lower in the presence of placentas from women with preeclampsia in comparison with physiological pregnancy.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>24771450</pmid><doi>10.1007/s10517-014-2397-0</doi><tpages>5</tpages></addata></record> |
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| subjects | Biomedical and Life Sciences Biomedicine Cell Biology Cell Culture Techniques Cell Line Cytokines Cytokines - metabolism Cytokines - secretion Female Humans Internal Medicine Laboratory Medicine Pathology Physiological aspects Placenta - physiology Pre-Eclampsia - immunology Pre-Eclampsia - metabolism Pregnancy Tissue Extracts - physiology Vascular endothelial growth factor |
| title | Effect of Factors Produced by the Placenta on Cytokine Secretion by THP-1 Cells Cultured on a 3D Scaffold |
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