High Affinity Interaction of Yeast Transcriptional Regulator, Mot1, with TATA Box-binding Protein (TBP)

Yeast Mot1, an essential ATP-dependent regulator of basal transcription, removes TATA box-binding protein (TBP) from TATA sites in vitro. Complexes of Mot1 and Spt15 (yeast TBP), radiolabeled in vitro, were immunoprecipitated with anti-TBP (or anti-Mot1) antibodies in the absence of DNA, showing Mot...

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Veröffentlicht in:	The Journal of biological chemistry 2001-04, Vol.276 (15), p.11883-11894
Hauptverfasser:	Adamkewicz, Joanne I., Hansen, Karin E., Prud'homme, Wendy A., Davis, Jennifer L., Thorner, Jeremy
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphatases Amino Acid Sequence Base Sequence DNA Helicases - metabolism DNA Primers DNA-Binding Proteins - chemistry DNA-Binding Proteins - metabolism dTRF1 protein Fluorescent Antibody Technique, Indirect Fungal Proteins - metabolism Molecular Sequence Data Mot1 protein Protein Binding Saccharomyces cerevisiae Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins TATA-Binding Protein Associated Factors TATA-Box Binding Protein Transcription Factors - chemistry Transcription Factors - metabolism
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