Sample processing effect on polymerase chain reaction used for identification of Campylobacter jejuni
Model samples of Campylobacter jejuni for polymerase chain reaction (PCR) were prepared by rapid and simple procedures consisting of centrifugation, proteinase K treatment, Chelex 100 treatment, and boiling lyses. A PCR based on specific amplification of the variable sequence of 16S rRNA gene was pe...
Gespeichert in:
| Veröffentlicht in: | Folia microbiologica 2004, Vol.49 (6), p.693-697 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 697 |
|---|---|
| container_issue | 6 |
| container_start_page | 693 |
| container_title | Folia microbiologica |
| container_volume | 49 |
| creator | Sabatkova, Z Pazlarova, J Demnerova, K |
| description | Model samples of Campylobacter jejuni for polymerase chain reaction (PCR) were prepared by rapid and simple procedures consisting of centrifugation, proteinase K treatment, Chelex 100 treatment, and boiling lyses. A PCR based on specific amplification of the variable sequence of 16S rRNA gene was performed using Tth DNA polymerase and the PCR products were visualized by agarose gel electrophoresis. The assay allowed the detection of 10 CFU/mL C. jejuni in the physiological saline and 100 CFU/mL in the basic Park and Sanders broth. |
| doi_str_mv | 10.1007/BF02931551 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_17843901</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17843901</sourcerecordid><originalsourceid>FETCH-LOGICAL-p240t-6c7188ae1076de9b091cfb488124556ac6cb9c0676f0faeecfbe5ee05d2505813</originalsourceid><addsrcrecordid>eNo1kDFPwzAUhD2AaCks_ADkiS3wnMSOM0JEAakSAzBHjvMMjhI72MnQf08KZTrp3afTuyPkisEtAyjuHraQlhnjnJ2QNQDjCRdZuiLnMXYAAvIsPSMrxqVkOfA1wTc1jD3SMXiNMVr3SdEY1BP1jo6-3w8YVESqv5R1NKDSk12cOWJLjQ_Utugma6xWv3dvaLUE7nvfLCQG2mE3O3tBTo3qI14edUM-to_v1XOye316qe53yZjmMCVCF0xKhQwK0WLZQMm0afLl1zTnXCgtdFNqEIUwYBTiYiJHBN6mHLhk2Ybc_OUudb5njFM92Kix75VDP8eaFTLPSjiA10dwbgZs6zHYQYV9_T9M9gMQgWQN</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17843901</pqid></control><display><type>article</type><title>Sample processing effect on polymerase chain reaction used for identification of Campylobacter jejuni</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Sabatkova, Z ; Pazlarova, J ; Demnerova, K</creator><creatorcontrib>Sabatkova, Z ; Pazlarova, J ; Demnerova, K</creatorcontrib><description>Model samples of Campylobacter jejuni for polymerase chain reaction (PCR) were prepared by rapid and simple procedures consisting of centrifugation, proteinase K treatment, Chelex 100 treatment, and boiling lyses. A PCR based on specific amplification of the variable sequence of 16S rRNA gene was performed using Tth DNA polymerase and the PCR products were visualized by agarose gel electrophoresis. The assay allowed the detection of 10 CFU/mL C. jejuni in the physiological saline and 100 CFU/mL in the basic Park and Sanders broth.</description><identifier>ISSN: 0015-5632</identifier><identifier>DOI: 10.1007/BF02931551</identifier><identifier>PMID: 15881405</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Campylobacter - classification ; Campylobacter - genetics ; Campylobacter - isolation & purification ; Campylobacter coli - classification ; Campylobacter coli - genetics ; Campylobacter coli - isolation & purification ; Campylobacter jejuni ; Campylobacter jejuni - classification ; Campylobacter jejuni - genetics ; Campylobacter jejuni - isolation & purification ; Culture Media ; Food Microbiology ; Humans ; Polymerase Chain Reaction - methods ; Sodium Chloride</subject><ispartof>Folia microbiologica, 2004, Vol.49 (6), p.693-697</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15881405$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sabatkova, Z</creatorcontrib><creatorcontrib>Pazlarova, J</creatorcontrib><creatorcontrib>Demnerova, K</creatorcontrib><title>Sample processing effect on polymerase chain reaction used for identification of Campylobacter jejuni</title><title>Folia microbiologica</title><addtitle>Folia Microbiol (Praha)</addtitle><description>Model samples of Campylobacter jejuni for polymerase chain reaction (PCR) were prepared by rapid and simple procedures consisting of centrifugation, proteinase K treatment, Chelex 100 treatment, and boiling lyses. A PCR based on specific amplification of the variable sequence of 16S rRNA gene was performed using Tth DNA polymerase and the PCR products were visualized by agarose gel electrophoresis. The assay allowed the detection of 10 CFU/mL C. jejuni in the physiological saline and 100 CFU/mL in the basic Park and Sanders broth.</description><subject>Animals</subject><subject>Campylobacter - classification</subject><subject>Campylobacter - genetics</subject><subject>Campylobacter - isolation & purification</subject><subject>Campylobacter coli - classification</subject><subject>Campylobacter coli - genetics</subject><subject>Campylobacter coli - isolation & purification</subject><subject>Campylobacter jejuni</subject><subject>Campylobacter jejuni - classification</subject><subject>Campylobacter jejuni - genetics</subject><subject>Campylobacter jejuni - isolation & purification</subject><subject>Culture Media</subject><subject>Food Microbiology</subject><subject>Humans</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Sodium Chloride</subject><issn>0015-5632</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kDFPwzAUhD2AaCks_ADkiS3wnMSOM0JEAakSAzBHjvMMjhI72MnQf08KZTrp3afTuyPkisEtAyjuHraQlhnjnJ2QNQDjCRdZuiLnMXYAAvIsPSMrxqVkOfA1wTc1jD3SMXiNMVr3SdEY1BP1jo6-3w8YVESqv5R1NKDSk12cOWJLjQ_Utugma6xWv3dvaLUE7nvfLCQG2mE3O3tBTo3qI14edUM-to_v1XOye316qe53yZjmMCVCF0xKhQwK0WLZQMm0afLl1zTnXCgtdFNqEIUwYBTiYiJHBN6mHLhk2Ybc_OUudb5njFM92Kix75VDP8eaFTLPSjiA10dwbgZs6zHYQYV9_T9M9gMQgWQN</recordid><startdate>2004</startdate><enddate>2004</enddate><creator>Sabatkova, Z</creator><creator>Pazlarova, J</creator><creator>Demnerova, K</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>2004</creationdate><title>Sample processing effect on polymerase chain reaction used for identification of Campylobacter jejuni</title><author>Sabatkova, Z ; Pazlarova, J ; Demnerova, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p240t-6c7188ae1076de9b091cfb488124556ac6cb9c0676f0faeecfbe5ee05d2505813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Campylobacter - classification</topic><topic>Campylobacter - genetics</topic><topic>Campylobacter - isolation & purification</topic><topic>Campylobacter coli - classification</topic><topic>Campylobacter coli - genetics</topic><topic>Campylobacter coli - isolation & purification</topic><topic>Campylobacter jejuni</topic><topic>Campylobacter jejuni - classification</topic><topic>Campylobacter jejuni - genetics</topic><topic>Campylobacter jejuni - isolation & purification</topic><topic>Culture Media</topic><topic>Food Microbiology</topic><topic>Humans</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Sodium Chloride</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sabatkova, Z</creatorcontrib><creatorcontrib>Pazlarova, J</creatorcontrib><creatorcontrib>Demnerova, K</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Folia microbiologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sabatkova, Z</au><au>Pazlarova, J</au><au>Demnerova, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sample processing effect on polymerase chain reaction used for identification of Campylobacter jejuni</atitle><jtitle>Folia microbiologica</jtitle><addtitle>Folia Microbiol (Praha)</addtitle><date>2004</date><risdate>2004</risdate><volume>49</volume><issue>6</issue><spage>693</spage><epage>697</epage><pages>693-697</pages><issn>0015-5632</issn><abstract>Model samples of Campylobacter jejuni for polymerase chain reaction (PCR) were prepared by rapid and simple procedures consisting of centrifugation, proteinase K treatment, Chelex 100 treatment, and boiling lyses. A PCR based on specific amplification of the variable sequence of 16S rRNA gene was performed using Tth DNA polymerase and the PCR products were visualized by agarose gel electrophoresis. The assay allowed the detection of 10 CFU/mL C. jejuni in the physiological saline and 100 CFU/mL in the basic Park and Sanders broth.</abstract><cop>United States</cop><pmid>15881405</pmid><doi>10.1007/BF02931551</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0015-5632 |
| ispartof | Folia microbiologica, 2004, Vol.49 (6), p.693-697 |
| issn | 0015-5632 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17843901 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Animals Campylobacter - classification Campylobacter - genetics Campylobacter - isolation & purification Campylobacter coli - classification Campylobacter coli - genetics Campylobacter coli - isolation & purification Campylobacter jejuni Campylobacter jejuni - classification Campylobacter jejuni - genetics Campylobacter jejuni - isolation & purification Culture Media Food Microbiology Humans Polymerase Chain Reaction - methods Sodium Chloride |
| title | Sample processing effect on polymerase chain reaction used for identification of Campylobacter jejuni |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-06T05%3A37%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Sample%20processing%20effect%20on%20polymerase%20chain%20reaction%20used%20for%20identification%20of%20Campylobacter%20jejuni&rft.jtitle=Folia%20microbiologica&rft.au=Sabatkova,%20Z&rft.date=2004&rft.volume=49&rft.issue=6&rft.spage=693&rft.epage=697&rft.pages=693-697&rft.issn=0015-5632&rft_id=info:doi/10.1007/BF02931551&rft_dat=%3Cproquest_pubme%3E17843901%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17843901&rft_id=info:pmid/15881405&rfr_iscdi=true |