Clinical and laboratory study of myleodysplastic syndrome (MDS)/myeloproliferative neoplasm (MPN) with PDGFRβ abnormalities
To explore the clinical and laboratory characteristics of myleodysplastic syndrome (MDS)/myeloproliferative neoplasm (MPN) with PDGFRβ abnormalities. Chromosome specimens were prepared directly and/or short-time culture of bone marrow cells. Karyotyping was performed with R-binding technique. Fluore...
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| Veröffentlicht in: | Zhōnghuá xuèyèxué zázhì 2010-08, Vol.31 (8), p.540-544 |
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| container_title | Zhōnghuá xuèyèxué zázhì |
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| creator | Gong, Sheng-Lan Qiu, Hui-Ying Song, Xian-Min Shao, Ru Wang, Jian-Min |
| description | To explore the clinical and laboratory characteristics of myleodysplastic syndrome (MDS)/myeloproliferative neoplasm (MPN) with PDGFRβ abnormalities.
Chromosome specimens were prepared directly and/or short-time culture of bone marrow cells. Karyotyping was performed with R-binding technique. Fluorescence in situ hybridization (FISH) was performed using PDGFRβ, PDGFRα, FGFR1 break-apart probes and whole chromosome 5 and 12 painting probes, respectively. The expression of JAK2 V617F was measured with quantitative PCR.
The clinical and hematological findings of 27 patients were compatible with diagnosis of MDS/MPN. PDGFRβ rearrangement was detected in 4 patients with D-FISH, and 2 of which were confirmed as t(5;12) by chromosome painting. PDGFRα, FGFR1 and JAK2 V617F mutation were not detected in these 4 PDGFRβ positive MDS/MPN patients with.
PDGFRβ gene rearrangement may be detected in some MDS/MPN patients. FISH is a convenient and reliable approach to detect PDGFRβ gene. |
| format | Article |
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Chromosome specimens were prepared directly and/or short-time culture of bone marrow cells. Karyotyping was performed with R-binding technique. Fluorescence in situ hybridization (FISH) was performed using PDGFRβ, PDGFRα, FGFR1 break-apart probes and whole chromosome 5 and 12 painting probes, respectively. The expression of JAK2 V617F was measured with quantitative PCR.
The clinical and hematological findings of 27 patients were compatible with diagnosis of MDS/MPN. PDGFRβ rearrangement was detected in 4 patients with D-FISH, and 2 of which were confirmed as t(5;12) by chromosome painting. PDGFRα, FGFR1 and JAK2 V617F mutation were not detected in these 4 PDGFRβ positive MDS/MPN patients with.
PDGFRβ gene rearrangement may be detected in some MDS/MPN patients. FISH is a convenient and reliable approach to detect PDGFRβ gene.</description><identifier>ISSN: 0253-2727</identifier><identifier>PMID: 21122334</identifier><language>chi</language><publisher>China</publisher><subject>Humans ; In Situ Hybridization, Fluorescence ; Karyotyping ; Myeloproliferative Disorders - genetics ; Neoplasms ; Receptor, Platelet-Derived Growth Factor beta - genetics</subject><ispartof>Zhōnghuá xuèyèxué zázhì, 2010-08, Vol.31 (8), p.540-544</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21122334$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gong, Sheng-Lan</creatorcontrib><creatorcontrib>Qiu, Hui-Ying</creatorcontrib><creatorcontrib>Song, Xian-Min</creatorcontrib><creatorcontrib>Shao, Ru</creatorcontrib><creatorcontrib>Wang, Jian-Min</creatorcontrib><title>Clinical and laboratory study of myleodysplastic syndrome (MDS)/myeloproliferative neoplasm (MPN) with PDGFRβ abnormalities</title><title>Zhōnghuá xuèyèxué zázhì</title><addtitle>Zhonghua Xue Ye Xue Za Zhi</addtitle><description>To explore the clinical and laboratory characteristics of myleodysplastic syndrome (MDS)/myeloproliferative neoplasm (MPN) with PDGFRβ abnormalities.
Chromosome specimens were prepared directly and/or short-time culture of bone marrow cells. Karyotyping was performed with R-binding technique. Fluorescence in situ hybridization (FISH) was performed using PDGFRβ, PDGFRα, FGFR1 break-apart probes and whole chromosome 5 and 12 painting probes, respectively. The expression of JAK2 V617F was measured with quantitative PCR.
The clinical and hematological findings of 27 patients were compatible with diagnosis of MDS/MPN. PDGFRβ rearrangement was detected in 4 patients with D-FISH, and 2 of which were confirmed as t(5;12) by chromosome painting. PDGFRα, FGFR1 and JAK2 V617F mutation were not detected in these 4 PDGFRβ positive MDS/MPN patients with.
PDGFRβ gene rearrangement may be detected in some MDS/MPN patients. FISH is a convenient and reliable approach to detect PDGFRβ gene.</description><subject>Humans</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Karyotyping</subject><subject>Myeloproliferative Disorders - genetics</subject><subject>Neoplasms</subject><subject>Receptor, Platelet-Derived Growth Factor beta - genetics</subject><issn>0253-2727</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kEtOwzAYhLMA0ar0CsjLdhHhR2InS5TSglSg4rGObOePsGTHIU5AkTgVB-FMBFFWs_lm9GlOojmmKYupoGIWLUMwCqeE8YwxfBbNKCGUMpbMo8_CmsZoaZFsKmSl8p3sfTei0A_ViHyN3GjBV2NorQy90SiMTdV5B2h1t3laX7oRrG87b00NU9W8A2rA_8JuIg73a_Rh-ld02Oy2j99fSKrGd05a0xsI59FpLW2A5TEX0cv2-rm4ifcPu9viah-3hPI-ljnnmQLKsoSmiiuNhUiAJoRjgrNEM6EqKTlPSIVBgsq10DWTaQK4TonQbBGt_nYnz7cBQl86EzRYKyfVIZREZCzHeZ7xCb04ooNyUJVtZ5zsxvL_MfYDLrVpfQ</recordid><startdate>201008</startdate><enddate>201008</enddate><creator>Gong, Sheng-Lan</creator><creator>Qiu, Hui-Ying</creator><creator>Song, Xian-Min</creator><creator>Shao, Ru</creator><creator>Wang, Jian-Min</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201008</creationdate><title>Clinical and laboratory study of myleodysplastic syndrome (MDS)/myeloproliferative neoplasm (MPN) with PDGFRβ abnormalities</title><author>Gong, Sheng-Lan ; Qiu, Hui-Ying ; Song, Xian-Min ; Shao, Ru ; Wang, Jian-Min</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p126t-a9668be238425b6bc0774e241601084c37bdaa6641d0eaeb9c7cf3a54e0f517c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2010</creationdate><topic>Humans</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Karyotyping</topic><topic>Myeloproliferative Disorders - genetics</topic><topic>Neoplasms</topic><topic>Receptor, Platelet-Derived Growth Factor beta - genetics</topic><toplevel>online_resources</toplevel><creatorcontrib>Gong, Sheng-Lan</creatorcontrib><creatorcontrib>Qiu, Hui-Ying</creatorcontrib><creatorcontrib>Song, Xian-Min</creatorcontrib><creatorcontrib>Shao, Ru</creatorcontrib><creatorcontrib>Wang, Jian-Min</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Zhōnghuá xuèyèxué zázhì</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gong, Sheng-Lan</au><au>Qiu, Hui-Ying</au><au>Song, Xian-Min</au><au>Shao, Ru</au><au>Wang, Jian-Min</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Clinical and laboratory study of myleodysplastic syndrome (MDS)/myeloproliferative neoplasm (MPN) with PDGFRβ abnormalities</atitle><jtitle>Zhōnghuá xuèyèxué zázhì</jtitle><addtitle>Zhonghua Xue Ye Xue Za Zhi</addtitle><date>2010-08</date><risdate>2010</risdate><volume>31</volume><issue>8</issue><spage>540</spage><epage>544</epage><pages>540-544</pages><issn>0253-2727</issn><abstract>To explore the clinical and laboratory characteristics of myleodysplastic syndrome (MDS)/myeloproliferative neoplasm (MPN) with PDGFRβ abnormalities.
Chromosome specimens were prepared directly and/or short-time culture of bone marrow cells. Karyotyping was performed with R-binding technique. Fluorescence in situ hybridization (FISH) was performed using PDGFRβ, PDGFRα, FGFR1 break-apart probes and whole chromosome 5 and 12 painting probes, respectively. The expression of JAK2 V617F was measured with quantitative PCR.
The clinical and hematological findings of 27 patients were compatible with diagnosis of MDS/MPN. PDGFRβ rearrangement was detected in 4 patients with D-FISH, and 2 of which were confirmed as t(5;12) by chromosome painting. PDGFRα, FGFR1 and JAK2 V617F mutation were not detected in these 4 PDGFRβ positive MDS/MPN patients with.
PDGFRβ gene rearrangement may be detected in some MDS/MPN patients. FISH is a convenient and reliable approach to detect PDGFRβ gene.</abstract><cop>China</cop><pmid>21122334</pmid><tpages>5</tpages></addata></record> |
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| ispartof | Zhōnghuá xuèyèxué zázhì, 2010-08, Vol.31 (8), p.540-544 |
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| language | chi |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Humans In Situ Hybridization, Fluorescence Karyotyping Myeloproliferative Disorders - genetics Neoplasms Receptor, Platelet-Derived Growth Factor beta - genetics |
| title | Clinical and laboratory study of myleodysplastic syndrome (MDS)/myeloproliferative neoplasm (MPN) with PDGFRβ abnormalities |
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