Cationic lipids enhance siRNA-mediated interferon response in mice
RNA interference mediated by small interfering RNAs (siRNAs) shows promise as a powerful research tool for gene function studies. However, controversy exists over the potential of siRNA-induced interferon response in vitro and in vivo. In this study, we showed that although intravenous administratio...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2005-05, Vol.330 (3), p.755-759 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Ma, Zheng Li, Jiang He, Fengtian Wilson, Annette Pitt, Bruce Li, Song |
| description | RNA interference mediated by small interfering RNAs (siRNAs) shows promise as a powerful research tool for gene function studies. However, controversy exists over the potential of siRNA-induced interferon response in vitro and in vivo. In this study, we showed that although intravenous administration of siRNA alone is essentially inert, injection of siRNA complexed with cationic liposomes resulted in a potent induction of both type I and type II interferon responses. Furthermore, i.v. administration of cationic lipid/siRNA complexes led to activation of STAT1. This study suggests caution in data interpretation and the potential toxicity with in vivo use of siRNA, particularly when delivered via a cationic lipid vector. This study also suggests the potential of siRNA as an immunostimulatory agent for immunotherapy. |
| doi_str_mv | 10.1016/j.bbrc.2005.03.041 |
| format | Article |
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However, controversy exists over the potential of siRNA-induced interferon response in vitro and in vivo. In this study, we showed that although intravenous administration of siRNA alone is essentially inert, injection of siRNA complexed with cationic liposomes resulted in a potent induction of both type I and type II interferon responses. Furthermore, i.v. administration of cationic lipid/siRNA complexes led to activation of STAT1. This study suggests caution in data interpretation and the potential toxicity with in vivo use of siRNA, particularly when delivered via a cationic lipid vector. This study also suggests the potential of siRNA as an immunostimulatory agent for immunotherapy.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2005.03.041</identifier><identifier>PMID: 15809061</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cationic liposome ; Cations - chemistry ; Cations - pharmacology ; Fatty Acids, Monounsaturated - chemistry ; Fatty Acids, Monounsaturated - pharmacology ; Female ; Innate immunity ; Interferon response ; Interferons - blood ; Interferons - genetics ; Interferons - metabolism ; Mice ; Mice, Inbred BALB C ; Quaternary Ammonium Compounds - chemistry ; Quaternary Ammonium Compounds - pharmacology ; Receptors, Cell Surface - genetics ; Receptors, Cell Surface - metabolism ; RNA interference ; RNA, Small Interfering - administration & dosage ; RNA, Small Interfering - genetics ; RNA, Small Interfering - pharmacology ; siRNA ; Toll-Like Receptor 4</subject><ispartof>Biochemical and biophysical research communications, 2005-05, Vol.330 (3), p.755-759</ispartof><rights>2005 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c385t-7bb69ccce08d83ed5ecef070469a5c09fca3dcab1c04d510c46cb04ecb239aa03</citedby><cites>FETCH-LOGICAL-c385t-7bb69ccce08d83ed5ecef070469a5c09fca3dcab1c04d510c46cb04ecb239aa03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X05005371$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15809061$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ma, Zheng</creatorcontrib><creatorcontrib>Li, Jiang</creatorcontrib><creatorcontrib>He, Fengtian</creatorcontrib><creatorcontrib>Wilson, Annette</creatorcontrib><creatorcontrib>Pitt, Bruce</creatorcontrib><creatorcontrib>Li, Song</creatorcontrib><title>Cationic lipids enhance siRNA-mediated interferon response in mice</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>RNA interference mediated by small interfering RNAs (siRNAs) shows promise as a powerful research tool for gene function studies. However, controversy exists over the potential of siRNA-induced interferon response in vitro and in vivo. In this study, we showed that although intravenous administration of siRNA alone is essentially inert, injection of siRNA complexed with cationic liposomes resulted in a potent induction of both type I and type II interferon responses. Furthermore, i.v. administration of cationic lipid/siRNA complexes led to activation of STAT1. This study suggests caution in data interpretation and the potential toxicity with in vivo use of siRNA, particularly when delivered via a cationic lipid vector. This study also suggests the potential of siRNA as an immunostimulatory agent for immunotherapy.</description><subject>Animals</subject><subject>Cationic liposome</subject><subject>Cations - chemistry</subject><subject>Cations - pharmacology</subject><subject>Fatty Acids, Monounsaturated - chemistry</subject><subject>Fatty Acids, Monounsaturated - pharmacology</subject><subject>Female</subject><subject>Innate immunity</subject><subject>Interferon response</subject><subject>Interferons - blood</subject><subject>Interferons - genetics</subject><subject>Interferons - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Quaternary Ammonium Compounds - chemistry</subject><subject>Quaternary Ammonium Compounds - pharmacology</subject><subject>Receptors, Cell Surface - genetics</subject><subject>Receptors, Cell Surface - metabolism</subject><subject>RNA interference</subject><subject>RNA, Small Interfering - administration & dosage</subject><subject>RNA, Small Interfering - genetics</subject><subject>RNA, Small Interfering - pharmacology</subject><subject>siRNA</subject><subject>Toll-Like Receptor 4</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEQhoMotlb_gAfZk7ddJ5vsdhe8aPELioIoeAvJZBZT9qMmW8F_b0oL3jwNDM_7MvMwds4h48DLq1VmjMcsBygyEBlIfsCmHGpIcw7ykE0BoEzzmn9M2EkIKwDOZVkfswkvqoiVfMpuF3p0Q-8wad3a2ZBQ_6l7pCS41-ebtCPr9Eg2cf1IviE_9ImnsB76QHGXdA7plB01ug10tp8z9n5_97Z4TJcvD0-Lm2WKoirGdG5MWSMiQWUrQbYgpAbmEC_SBULdoBYWteEI0hYcUJZoQBKaXNRag5ixy13v2g9fGwqj6lxAalvd07AJis8rUUouI5jvQPRDCJ4atfau0_5HcVBbc2qltubU1pwCoaK5GLrYt29M_PovslcVgesdQPHHb0deBXQUVVnnCUdlB_df_y85hIAN</recordid><startdate>20050513</startdate><enddate>20050513</enddate><creator>Ma, Zheng</creator><creator>Li, Jiang</creator><creator>He, Fengtian</creator><creator>Wilson, Annette</creator><creator>Pitt, Bruce</creator><creator>Li, Song</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope></search><sort><creationdate>20050513</creationdate><title>Cationic lipids enhance siRNA-mediated interferon response in mice</title><author>Ma, Zheng ; Li, Jiang ; He, Fengtian ; Wilson, Annette ; Pitt, Bruce ; Li, Song</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c385t-7bb69ccce08d83ed5ecef070469a5c09fca3dcab1c04d510c46cb04ecb239aa03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Cationic liposome</topic><topic>Cations - chemistry</topic><topic>Cations - pharmacology</topic><topic>Fatty Acids, Monounsaturated - chemistry</topic><topic>Fatty Acids, Monounsaturated - pharmacology</topic><topic>Female</topic><topic>Innate immunity</topic><topic>Interferon response</topic><topic>Interferons - blood</topic><topic>Interferons - genetics</topic><topic>Interferons - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Quaternary Ammonium Compounds - chemistry</topic><topic>Quaternary Ammonium Compounds - pharmacology</topic><topic>Receptors, Cell Surface - genetics</topic><topic>Receptors, Cell Surface - metabolism</topic><topic>RNA interference</topic><topic>RNA, Small Interfering - administration & dosage</topic><topic>RNA, Small Interfering - genetics</topic><topic>RNA, Small Interfering - pharmacology</topic><topic>siRNA</topic><topic>Toll-Like Receptor 4</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ma, Zheng</creatorcontrib><creatorcontrib>Li, Jiang</creatorcontrib><creatorcontrib>He, Fengtian</creatorcontrib><creatorcontrib>Wilson, Annette</creatorcontrib><creatorcontrib>Pitt, Bruce</creatorcontrib><creatorcontrib>Li, Song</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ma, Zheng</au><au>Li, Jiang</au><au>He, Fengtian</au><au>Wilson, Annette</au><au>Pitt, Bruce</au><au>Li, Song</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cationic lipids enhance siRNA-mediated interferon response in mice</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2005-05-13</date><risdate>2005</risdate><volume>330</volume><issue>3</issue><spage>755</spage><epage>759</epage><pages>755-759</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>RNA interference mediated by small interfering RNAs (siRNAs) shows promise as a powerful research tool for gene function studies. However, controversy exists over the potential of siRNA-induced interferon response in vitro and in vivo. In this study, we showed that although intravenous administration of siRNA alone is essentially inert, injection of siRNA complexed with cationic liposomes resulted in a potent induction of both type I and type II interferon responses. Furthermore, i.v. administration of cationic lipid/siRNA complexes led to activation of STAT1. This study suggests caution in data interpretation and the potential toxicity with in vivo use of siRNA, particularly when delivered via a cationic lipid vector. This study also suggests the potential of siRNA as an immunostimulatory agent for immunotherapy.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>15809061</pmid><doi>10.1016/j.bbrc.2005.03.041</doi><tpages>5</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 2005-05, Vol.330 (3), p.755-759 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Cationic liposome Cations - chemistry Cations - pharmacology Fatty Acids, Monounsaturated - chemistry Fatty Acids, Monounsaturated - pharmacology Female Innate immunity Interferon response Interferons - blood Interferons - genetics Interferons - metabolism Mice Mice, Inbred BALB C Quaternary Ammonium Compounds - chemistry Quaternary Ammonium Compounds - pharmacology Receptors, Cell Surface - genetics Receptors, Cell Surface - metabolism RNA interference RNA, Small Interfering - administration & dosage RNA, Small Interfering - genetics RNA, Small Interfering - pharmacology siRNA Toll-Like Receptor 4 |
| title | Cationic lipids enhance siRNA-mediated interferon response in mice |
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