Peptide-Mediated Specific Immobilization of Catalytically Active Cytochrome P450 BM3 Variant

Cytochrome P450 BM3 (CYP102A1) from Bacillus megaterium is an interesting target for biotechnological applications, because of its vast substrate variety combined with high P450 monooxygenase activity. The low stability in vitro could be overcome by immobilization on surfaces. Here we describe a nov...

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Veröffentlicht in:	Bioconjugate chemistry 2016-04, Vol.27 (4), p.1090-1097
Hauptverfasser:	Zernia, Sarah, Ott, Florian, Bellmann-Sickert, Kathrin, Frank, Ronny, Klenner, Marcus, Jahnke, Heinz-Georg, Prager, Andrea, Abel, Bernd, Robitzki, Andrea, Beck-Sickinger, Annette G
Format:	Artikel
Sprache:	eng
Schlagworte:	Biotechnology Catalysis Chromatography, High Pressure Liquid Cytochrome Cytochrome P-450 Enzyme System - metabolism Electrophoresis, Polyacrylamide Gel Enzymes, Immobilized - metabolism Gram-positive bacteria Peptides Peptides - metabolism Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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