Heat shock-regulated site-specific excision of extraneous DNA in transgenic plants
We have developed a heat shock-inducible, site-specific DNA excision system in transgenic plants mediated by the Cre/ loxP DNA recombination system. The heat shock-inducible promoter, HSP81-1, tightly controlled the expression of the Cre recombinase. Upon induction by heat shock, extraneous sequence...
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| Veröffentlicht in: | Plant science (Limerick) 2005-04, Vol.168 (4), p.997-1003 |
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| container_title | Plant science (Limerick) |
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| creator | Liu, Hai-Kun Yang, Chao Wei, Zhi-Ming |
| description | We have developed a heat shock-inducible, site-specific DNA excision system in transgenic plants mediated by the Cre/
loxP DNA recombination system. The heat shock-inducible promoter,
HSP81-1, tightly controlled the expression of the Cre recombinase. Upon induction by heat shock, extraneous sequences (the selectable marker, the undesirable
bar gene, NLS, Cre, and HSP flanked by two
loxP sites) were excised from the tobacco genome, leading to activation of the downstream GUS reporter gene. Genetic and molecular analyses indicate that the system is tightly controlled, showing high-efficiency inducible DNA excision in 17 transgenic lines. This system provides a highly reliable method for the removal of sequences that have fulfilled their duties in the generation of transgenic plants, thus creating transgenic plants free from useless exogenous DNA. |
| doi_str_mv | 10.1016/j.plantsci.2004.11.021 |
| format | Article |
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loxP DNA recombination system. The heat shock-inducible promoter,
HSP81-1, tightly controlled the expression of the Cre recombinase. Upon induction by heat shock, extraneous sequences (the selectable marker, the undesirable
bar gene, NLS, Cre, and HSP flanked by two
loxP sites) were excised from the tobacco genome, leading to activation of the downstream GUS reporter gene. Genetic and molecular analyses indicate that the system is tightly controlled, showing high-efficiency inducible DNA excision in 17 transgenic lines. This system provides a highly reliable method for the removal of sequences that have fulfilled their duties in the generation of transgenic plants, thus creating transgenic plants free from useless exogenous DNA.</description><identifier>ISSN: 0168-9452</identifier><identifier>EISSN: 1873-2259</identifier><identifier>DOI: 10.1016/j.plantsci.2004.11.021</identifier><identifier>CODEN: PLSCE4</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Agronomy. Soil science and plant productions ; beta-glucuronidase ; Biological and medical sciences ; Cre recombinase ; Cre/ loxP ; DNA damage ; DNA excision ; enzyme activity ; Extraneous DNA ; Fundamental and applied biological sciences. Psychology ; Genetic engineering applications ; genetic recombination ; genetic transformation ; Genetics and breeding of economic plants ; heat stress ; HSAE ; HSP ; molecular sequence data ; Nicotiana tabacum ; nucleotide sequences ; Plant breeding: fundamental aspects and methodology ; plasmids ; promoter regions ; tobacco ; Transgenic plants</subject><ispartof>Plant science (Limerick), 2005-04, Vol.168 (4), p.997-1003</ispartof><rights>2004 Elsevier Ireland Ltd</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c397t-740519f06b78ea0b145e4780d162cee9c2c75e4be35f386891ac50d8dd5a43ee3</citedby><cites>FETCH-LOGICAL-c397t-740519f06b78ea0b145e4780d162cee9c2c75e4be35f386891ac50d8dd5a43ee3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0168945204004881$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16568775$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Hai-Kun</creatorcontrib><creatorcontrib>Yang, Chao</creatorcontrib><creatorcontrib>Wei, Zhi-Ming</creatorcontrib><title>Heat shock-regulated site-specific excision of extraneous DNA in transgenic plants</title><title>Plant science (Limerick)</title><description>We have developed a heat shock-inducible, site-specific DNA excision system in transgenic plants mediated by the Cre/
loxP DNA recombination system. The heat shock-inducible promoter,
HSP81-1, tightly controlled the expression of the Cre recombinase. Upon induction by heat shock, extraneous sequences (the selectable marker, the undesirable
bar gene, NLS, Cre, and HSP flanked by two
loxP sites) were excised from the tobacco genome, leading to activation of the downstream GUS reporter gene. Genetic and molecular analyses indicate that the system is tightly controlled, showing high-efficiency inducible DNA excision in 17 transgenic lines. This system provides a highly reliable method for the removal of sequences that have fulfilled their duties in the generation of transgenic plants, thus creating transgenic plants free from useless exogenous DNA.</description><subject>Agronomy. Soil science and plant productions</subject><subject>beta-glucuronidase</subject><subject>Biological and medical sciences</subject><subject>Cre recombinase</subject><subject>Cre/ loxP</subject><subject>DNA damage</subject><subject>DNA excision</subject><subject>enzyme activity</subject><subject>Extraneous DNA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering applications</subject><subject>genetic recombination</subject><subject>genetic transformation</subject><subject>Genetics and breeding of economic plants</subject><subject>heat stress</subject><subject>HSAE</subject><subject>HSP</subject><subject>molecular sequence data</subject><subject>Nicotiana tabacum</subject><subject>nucleotide sequences</subject><subject>Plant breeding: fundamental aspects and methodology</subject><subject>plasmids</subject><subject>promoter regions</subject><subject>tobacco</subject><subject>Transgenic plants</subject><issn>0168-9452</issn><issn>1873-2259</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqFkE1P3DAQhi3USt1S_kLJBW4JM3YcOzfQ8imtWqnA2fI6k623IdnaWVT-PY5CxZGTx9YzM68fxr4jFAhYnW2LXWf7MTpfcICyQCyA4wFboFYi51zWn9gigTqvS8m_sK8xbgGAS6kW7Nct2TGLvwf3Jw-02Xd2pCaLfqQ87sj51ruM_jkf_dBnQ5vqMdiehn3MLn9cZL7PpnvcUJ_AOcc39rm1XaSjt_OQPV5fPSxv89XPm7vlxSp3olZjrkqQWLdQrZUmC2ssJZVKQ4MVd0S1406llzUJ2Qpd6Rqtk9DoppG2FETikJ3Oc3dh-LunOJonHx113ZzPoNIChYAEVjPowhBjoNbsgn-y4cUgmEmh2Zr_Cs2k0CCapDA1nrxtsNHZrk0_TSbeuytZaaVk4o5nrrWDsZuQmMd7DigAaqXKaiLOZ4KSkGdPwaRd1DtqfCA3mmbwH4V5BbnVlHU</recordid><startdate>20050401</startdate><enddate>20050401</enddate><creator>Liu, Hai-Kun</creator><creator>Yang, Chao</creator><creator>Wei, Zhi-Ming</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20050401</creationdate><title>Heat shock-regulated site-specific excision of extraneous DNA in transgenic plants</title><author>Liu, Hai-Kun ; Yang, Chao ; Wei, Zhi-Ming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c397t-740519f06b78ea0b145e4780d162cee9c2c75e4be35f386891ac50d8dd5a43ee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>beta-glucuronidase</topic><topic>Biological and medical sciences</topic><topic>Cre recombinase</topic><topic>Cre/ loxP</topic><topic>DNA damage</topic><topic>DNA excision</topic><topic>enzyme activity</topic><topic>Extraneous DNA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic engineering applications</topic><topic>genetic recombination</topic><topic>genetic transformation</topic><topic>Genetics and breeding of economic plants</topic><topic>heat stress</topic><topic>HSAE</topic><topic>HSP</topic><topic>molecular sequence data</topic><topic>Nicotiana tabacum</topic><topic>nucleotide sequences</topic><topic>Plant breeding: fundamental aspects and methodology</topic><topic>plasmids</topic><topic>promoter regions</topic><topic>tobacco</topic><topic>Transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Hai-Kun</creatorcontrib><creatorcontrib>Yang, Chao</creatorcontrib><creatorcontrib>Wei, Zhi-Ming</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Plant science (Limerick)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Hai-Kun</au><au>Yang, Chao</au><au>Wei, Zhi-Ming</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Heat shock-regulated site-specific excision of extraneous DNA in transgenic plants</atitle><jtitle>Plant science (Limerick)</jtitle><date>2005-04-01</date><risdate>2005</risdate><volume>168</volume><issue>4</issue><spage>997</spage><epage>1003</epage><pages>997-1003</pages><issn>0168-9452</issn><eissn>1873-2259</eissn><coden>PLSCE4</coden><abstract>We have developed a heat shock-inducible, site-specific DNA excision system in transgenic plants mediated by the Cre/
loxP DNA recombination system. The heat shock-inducible promoter,
HSP81-1, tightly controlled the expression of the Cre recombinase. Upon induction by heat shock, extraneous sequences (the selectable marker, the undesirable
bar gene, NLS, Cre, and HSP flanked by two
loxP sites) were excised from the tobacco genome, leading to activation of the downstream GUS reporter gene. Genetic and molecular analyses indicate that the system is tightly controlled, showing high-efficiency inducible DNA excision in 17 transgenic lines. This system provides a highly reliable method for the removal of sequences that have fulfilled their duties in the generation of transgenic plants, thus creating transgenic plants free from useless exogenous DNA.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><doi>10.1016/j.plantsci.2004.11.021</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0168-9452 |
| ispartof | Plant science (Limerick), 2005-04, Vol.168 (4), p.997-1003 |
| issn | 0168-9452 1873-2259 |
| language | eng |
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| source | Elsevier ScienceDirect Journals |
| subjects | Agronomy. Soil science and plant productions beta-glucuronidase Biological and medical sciences Cre recombinase Cre/ loxP DNA damage DNA excision enzyme activity Extraneous DNA Fundamental and applied biological sciences. Psychology Genetic engineering applications genetic recombination genetic transformation Genetics and breeding of economic plants heat stress HSAE HSP molecular sequence data Nicotiana tabacum nucleotide sequences Plant breeding: fundamental aspects and methodology plasmids promoter regions tobacco Transgenic plants |
| title | Heat shock-regulated site-specific excision of extraneous DNA in transgenic plants |
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