A new cytotoxin from Bacillus cereus that may cause necrotic enteritis
A cytotoxin (CytK) has been isolated from a Bacillus cereus strain that caused a severe food poisoning outbreak killing three people. A protein of 34 kDa was highly cytotoxic, and the addition of other secreted proteins gave no synergistic effect. CytK was also necrotic and haemolytic. No known B. c...
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| Veröffentlicht in: | Molecular microbiology 2000-10, Vol.38 (2), p.254-261 |
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| creator | Lund, Terje De Buyser, Marie‐Laure Granum, Per Einar |
| description | A cytotoxin (CytK) has been isolated from a Bacillus cereus strain that caused a severe food poisoning outbreak killing three people. A protein of 34 kDa was highly cytotoxic, and the addition of other secreted proteins gave no synergistic effect. CytK was also necrotic and haemolytic. No known B. cereus enterotoxins were produced by this strain. A DNA sequence from 1.8 kb upstream to 0.2 kb downstream of the toxin gene was sequenced. The deduced amino acid sequence of the toxin showed similarity to Staphylococcus aureus leucocidins, γ‐haemolysin and α‐haemolysin, Clostridium perfringensβ‐toxin and B. cereus haemolysin II, all belonging to a family of β‐barrel channel‐forming toxins. There was no sequence similarity between CytK and enterotoxins of B. cereus. The upstream sequence contained a partial sequence of a putative histidine kinase gene. A recognition site for PlcR, which regulates the transcription of enterotoxins HBL and Nhe of B. cereus, was found in the promoter region of the toxin. This new cytotoxin may be responsible for a disease that is similar to, although not as severe as, the necrotic enteritis caused by the β‐toxin of C. perfringens type C. |
| doi_str_mv | 10.1046/j.1365-2958.2000.02147.x |
| format | Article |
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A protein of 34 kDa was highly cytotoxic, and the addition of other secreted proteins gave no synergistic effect. CytK was also necrotic and haemolytic. No known B. cereus enterotoxins were produced by this strain. A DNA sequence from 1.8 kb upstream to 0.2 kb downstream of the toxin gene was sequenced. The deduced amino acid sequence of the toxin showed similarity to Staphylococcus aureus leucocidins, γ‐haemolysin and α‐haemolysin, Clostridium perfringensβ‐toxin and B. cereus haemolysin II, all belonging to a family of β‐barrel channel‐forming toxins. There was no sequence similarity between CytK and enterotoxins of B. cereus. The upstream sequence contained a partial sequence of a putative histidine kinase gene. A recognition site for PlcR, which regulates the transcription of enterotoxins HBL and Nhe of B. cereus, was found in the promoter region of the toxin. This new cytotoxin may be responsible for a disease that is similar to, although not as severe as, the necrotic enteritis caused by the β‐toxin of C. perfringens type C.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1046/j.1365-2958.2000.02147.x</identifier><identifier>PMID: 11069652</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>a-haemolysin ; a-Hemolysin ; Amino Acid Sequence ; Animals ; b-toxin ; Bacillus cereus ; Bacillus cereus - genetics ; Bacterial Toxins - genetics ; Bacterial Toxins - isolation & purification ; Bacterial Toxins - metabolism ; Base Sequence ; Cercopithecus aethiops ; CytK protein ; cytotoxin CytK ; cytotoxins ; Cytotoxins - genetics ; Cytotoxins - isolation & purification ; Cytotoxins - metabolism ; Disease Outbreaks ; DNA, Bacterial ; Enteritis ; Enterotoxins - genetics ; Enterotoxins - isolation & purification ; Enterotoxins - metabolism ; Foodborne Diseases - epidemiology ; Foodborne Diseases - microbiology ; France - epidemiology ; g-haemolysin ; g-Hemolysin ; HBL protein ; hemolysin II ; Hemolysis ; Humans ; leucocidin ; Molecular Sequence Data ; Necrosis ; Nhe protein ; PlcR protein ; Rabbits ; Sequence Homology, Amino Acid ; Vero Cells</subject><ispartof>Molecular microbiology, 2000-10, Vol.38 (2), p.254-261</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5017-913f0656fa8535382046503b33d7f1ad8904572064faefffb12c93e00f704beb3</citedby><cites>FETCH-LOGICAL-c5017-913f0656fa8535382046503b33d7f1ad8904572064faefffb12c93e00f704beb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2958.2000.02147.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2958.2000.02147.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11069652$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lund, Terje</creatorcontrib><creatorcontrib>De Buyser, Marie‐Laure</creatorcontrib><creatorcontrib>Granum, Per Einar</creatorcontrib><title>A new cytotoxin from Bacillus cereus that may cause necrotic enteritis</title><title>Molecular microbiology</title><addtitle>Mol Microbiol</addtitle><description>A cytotoxin (CytK) has been isolated from a Bacillus cereus strain that caused a severe food poisoning outbreak killing three people. A protein of 34 kDa was highly cytotoxic, and the addition of other secreted proteins gave no synergistic effect. CytK was also necrotic and haemolytic. No known B. cereus enterotoxins were produced by this strain. A DNA sequence from 1.8 kb upstream to 0.2 kb downstream of the toxin gene was sequenced. The deduced amino acid sequence of the toxin showed similarity to Staphylococcus aureus leucocidins, γ‐haemolysin and α‐haemolysin, Clostridium perfringensβ‐toxin and B. cereus haemolysin II, all belonging to a family of β‐barrel channel‐forming toxins. There was no sequence similarity between CytK and enterotoxins of B. cereus. The upstream sequence contained a partial sequence of a putative histidine kinase gene. A recognition site for PlcR, which regulates the transcription of enterotoxins HBL and Nhe of B. cereus, was found in the promoter region of the toxin. This new cytotoxin may be responsible for a disease that is similar to, although not as severe as, the necrotic enteritis caused by the β‐toxin of C. perfringens type C.</description><subject>a-haemolysin</subject><subject>a-Hemolysin</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>b-toxin</subject><subject>Bacillus cereus</subject><subject>Bacillus cereus - genetics</subject><subject>Bacterial Toxins - genetics</subject><subject>Bacterial Toxins - isolation & purification</subject><subject>Bacterial Toxins - metabolism</subject><subject>Base Sequence</subject><subject>Cercopithecus aethiops</subject><subject>CytK protein</subject><subject>cytotoxin CytK</subject><subject>cytotoxins</subject><subject>Cytotoxins - genetics</subject><subject>Cytotoxins - isolation & purification</subject><subject>Cytotoxins - metabolism</subject><subject>Disease Outbreaks</subject><subject>DNA, Bacterial</subject><subject>Enteritis</subject><subject>Enterotoxins - genetics</subject><subject>Enterotoxins - isolation & purification</subject><subject>Enterotoxins - metabolism</subject><subject>Foodborne Diseases - epidemiology</subject><subject>Foodborne Diseases - microbiology</subject><subject>France - epidemiology</subject><subject>g-haemolysin</subject><subject>g-Hemolysin</subject><subject>HBL protein</subject><subject>hemolysin II</subject><subject>Hemolysis</subject><subject>Humans</subject><subject>leucocidin</subject><subject>Molecular Sequence Data</subject><subject>Necrosis</subject><subject>Nhe protein</subject><subject>PlcR protein</subject><subject>Rabbits</subject><subject>Sequence Homology, Amino Acid</subject><subject>Vero Cells</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE9PwzAMxSMEYmPwFVBO3FqcpumfA4cxMZi0iQtI3KI0c0Sndh1Jqq3fnpZNcOVkS37Pfv4RQhmEDOLkfhMynoggykUWRgAQQsTiNDyckfHv4JyMIRcQ8Cz6GJEr5zYAjEPCL8mIMUjyRERjMp_SLe6p7nzjm0O5pcY2NX1Uuqyq1lGNFvviP5WnteqoVq3D3qFt40tNcevRlr501-TCqMrhzalOyPv86W32Eixfnxez6TLQAlga5IwbSERiVCa46JP1zwjgBefr1DC1znKIRRpBEhuFxpiCRTrnCGBSiAss-ITcHffubPPVovOyLp3GqlJbbFonWZpxSCPeC7OjsE_qnEUjd7asle0kAzkwlBs5oJIDKjkwlD8M5aG33p5utEWN6z_jCVoveDgK9mWF3b8Xy9VqMXT8G-0Ifx8</recordid><startdate>200010</startdate><enddate>200010</enddate><creator>Lund, Terje</creator><creator>De Buyser, Marie‐Laure</creator><creator>Granum, Per Einar</creator><general>Blackwell Science Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>200010</creationdate><title>A new cytotoxin from Bacillus cereus that may cause necrotic enteritis</title><author>Lund, Terje ; De Buyser, Marie‐Laure ; Granum, Per Einar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5017-913f0656fa8535382046503b33d7f1ad8904572064faefffb12c93e00f704beb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>a-haemolysin</topic><topic>a-Hemolysin</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>b-toxin</topic><topic>Bacillus cereus</topic><topic>Bacillus cereus - genetics</topic><topic>Bacterial Toxins - genetics</topic><topic>Bacterial Toxins - isolation & purification</topic><topic>Bacterial Toxins - metabolism</topic><topic>Base Sequence</topic><topic>Cercopithecus aethiops</topic><topic>CytK protein</topic><topic>cytotoxin CytK</topic><topic>cytotoxins</topic><topic>Cytotoxins - genetics</topic><topic>Cytotoxins - isolation & purification</topic><topic>Cytotoxins - metabolism</topic><topic>Disease Outbreaks</topic><topic>DNA, Bacterial</topic><topic>Enteritis</topic><topic>Enterotoxins - genetics</topic><topic>Enterotoxins - isolation & purification</topic><topic>Enterotoxins - metabolism</topic><topic>Foodborne Diseases - epidemiology</topic><topic>Foodborne Diseases - microbiology</topic><topic>France - epidemiology</topic><topic>g-haemolysin</topic><topic>g-Hemolysin</topic><topic>HBL protein</topic><topic>hemolysin II</topic><topic>Hemolysis</topic><topic>Humans</topic><topic>leucocidin</topic><topic>Molecular Sequence Data</topic><topic>Necrosis</topic><topic>Nhe protein</topic><topic>PlcR protein</topic><topic>Rabbits</topic><topic>Sequence Homology, Amino Acid</topic><topic>Vero Cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lund, Terje</creatorcontrib><creatorcontrib>De Buyser, Marie‐Laure</creatorcontrib><creatorcontrib>Granum, Per Einar</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lund, Terje</au><au>De Buyser, Marie‐Laure</au><au>Granum, Per Einar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A new cytotoxin from Bacillus cereus that may cause necrotic enteritis</atitle><jtitle>Molecular microbiology</jtitle><addtitle>Mol Microbiol</addtitle><date>2000-10</date><risdate>2000</risdate><volume>38</volume><issue>2</issue><spage>254</spage><epage>261</epage><pages>254-261</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>A cytotoxin (CytK) has been isolated from a Bacillus cereus strain that caused a severe food poisoning outbreak killing three people. A protein of 34 kDa was highly cytotoxic, and the addition of other secreted proteins gave no synergistic effect. CytK was also necrotic and haemolytic. No known B. cereus enterotoxins were produced by this strain. A DNA sequence from 1.8 kb upstream to 0.2 kb downstream of the toxin gene was sequenced. The deduced amino acid sequence of the toxin showed similarity to Staphylococcus aureus leucocidins, γ‐haemolysin and α‐haemolysin, Clostridium perfringensβ‐toxin and B. cereus haemolysin II, all belonging to a family of β‐barrel channel‐forming toxins. There was no sequence similarity between CytK and enterotoxins of B. cereus. The upstream sequence contained a partial sequence of a putative histidine kinase gene. A recognition site for PlcR, which regulates the transcription of enterotoxins HBL and Nhe of B. cereus, was found in the promoter region of the toxin. This new cytotoxin may be responsible for a disease that is similar to, although not as severe as, the necrotic enteritis caused by the β‐toxin of C. perfringens type C.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>11069652</pmid><doi>10.1046/j.1365-2958.2000.02147.x</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | a-haemolysin a-Hemolysin Amino Acid Sequence Animals b-toxin Bacillus cereus Bacillus cereus - genetics Bacterial Toxins - genetics Bacterial Toxins - isolation & purification Bacterial Toxins - metabolism Base Sequence Cercopithecus aethiops CytK protein cytotoxin CytK cytotoxins Cytotoxins - genetics Cytotoxins - isolation & purification Cytotoxins - metabolism Disease Outbreaks DNA, Bacterial Enteritis Enterotoxins - genetics Enterotoxins - isolation & purification Enterotoxins - metabolism Foodborne Diseases - epidemiology Foodborne Diseases - microbiology France - epidemiology g-haemolysin g-Hemolysin HBL protein hemolysin II Hemolysis Humans leucocidin Molecular Sequence Data Necrosis Nhe protein PlcR protein Rabbits Sequence Homology, Amino Acid Vero Cells |
| title | A new cytotoxin from Bacillus cereus that may cause necrotic enteritis |
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