Antibody-dependent enhancement of heterotypic dengue infections involved in suppression of IFNγ production
Antibody‐dependent enhancement has been implicated in some outbreaks of epidemic dengue hemorrhagic fever, however, the mechanism of antibody‐dependent enhancement is not well known. This study was conducted to investigate the cross‐protection and cross‐enhancement of dengue‐2 virus infections by de...
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| Veröffentlicht in: | Journal of medical virology 2001-02, Vol.63 (2), p.150-157 |
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| creator | Yang, Kuender D. Yeh, Wen-Ting Yang, Ming-Yu Chen, Rong-Fu Shaio, Men-Fang |
| description | Antibody‐dependent enhancement has been implicated in some outbreaks of epidemic dengue hemorrhagic fever, however, the mechanism of antibody‐dependent enhancement is not well known. This study was conducted to investigate the cross‐protection and cross‐enhancement of dengue‐2 virus infections by dengue‐1 immune sera. It was found that dengue‐1 immune sera at 1:5 dilution (n = 12) could neutralize dengue‐2 infections in BHK‐21 cells, as assessed by a standard plaque‐reduction neutralization assay. Two‐thirds of the dengue‐1 immune sera at 1:25 dilution demonstrated neutralizing effects for dengue‐2 infections, whereas, non‐immune sera revealed no neutralization for dengue‐2 infections in BHK‐21 cells. Human mononuclear leukocytes in response to dengue‐2 infections elicited a T cell helper 1 (Th1) response revealing induction of IFNγ but not IL‐4 production. Dengue‐1 immune sera did not neutralize dengue‐2 infections in mononuclear leukocytes. Subneutralizing titers of dengue‐1 immune sera at 1:250, but not at 1:10 dilution, enhanced dengue‐2 infections in mononuclear leukocytes (1.2 ± 0.7 × 104 vs. 2.8 ± 0.3 × 102 PFU/ml). The enhancement of dengue‐2 infections in mononuclear leukocytes by dengue‐1 immune sera at 1:250 was associated with an increase in the lymphocyte proliferation index, and a decrease in IFNγ production (56 ± 24 vs. 12 ± 3 pg/ml). The addition of IFNγ (0.1 μg/ml) suppressed significantly the antibody‐dependent enhancement induced by dengue‐1 immune sera, whereas the presence of anti‐IFNγ F(ab)2 antibody augmented the antibody‐dependent enhancement effect. Results from this study suggest that suppression of Th1 response may be involved in the antibody‐dependent enhancement of heterotypic dengue infections. Better regulation of Th1/Th2 reactions may be useful for prevention of heterotypic immune enhancement of dengue infections. J. Med. Virol. 63:150–157, 2001. © 2001 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/1096-9071(20000201)63:2<150::AID-JMV1010>3.0.CO;2-A |
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This study was conducted to investigate the cross‐protection and cross‐enhancement of dengue‐2 virus infections by dengue‐1 immune sera. It was found that dengue‐1 immune sera at 1:5 dilution (n = 12) could neutralize dengue‐2 infections in BHK‐21 cells, as assessed by a standard plaque‐reduction neutralization assay. Two‐thirds of the dengue‐1 immune sera at 1:25 dilution demonstrated neutralizing effects for dengue‐2 infections, whereas, non‐immune sera revealed no neutralization for dengue‐2 infections in BHK‐21 cells. Human mononuclear leukocytes in response to dengue‐2 infections elicited a T cell helper 1 (Th1) response revealing induction of IFNγ but not IL‐4 production. Dengue‐1 immune sera did not neutralize dengue‐2 infections in mononuclear leukocytes. Subneutralizing titers of dengue‐1 immune sera at 1:250, but not at 1:10 dilution, enhanced dengue‐2 infections in mononuclear leukocytes (1.2 ± 0.7 × 104 vs. 2.8 ± 0.3 × 102 PFU/ml). The enhancement of dengue‐2 infections in mononuclear leukocytes by dengue‐1 immune sera at 1:250 was associated with an increase in the lymphocyte proliferation index, and a decrease in IFNγ production (56 ± 24 vs. 12 ± 3 pg/ml). The addition of IFNγ (0.1 μg/ml) suppressed significantly the antibody‐dependent enhancement induced by dengue‐1 immune sera, whereas the presence of anti‐IFNγ F(ab)2 antibody augmented the antibody‐dependent enhancement effect. Results from this study suggest that suppression of Th1 response may be involved in the antibody‐dependent enhancement of heterotypic dengue infections. Better regulation of Th1/Th2 reactions may be useful for prevention of heterotypic immune enhancement of dengue infections. J. Med. Virol. 63:150–157, 2001. © 2001 Wiley‐Liss, Inc.</description><identifier>ISSN: 0146-6615</identifier><identifier>EISSN: 1096-9071</identifier><identifier>DOI: 10.1002/1096-9071(20000201)63:2<150::AID-JMV1010>3.0.CO;2-A</identifier><identifier>CODEN: JMVIDB</identifier><language>eng</language><publisher>New York: John Wiley & Sons, Inc</publisher><subject>antibody-dependent enhancement ; Arboviroses ; Biological and medical sciences ; Dengue fevers ; dengue hemorrhagic fever ; Dengue virus 2 ; g-Interferon ; Human viral diseases ; Infectious diseases ; Medical sciences ; plaque-forming units (PFU) ; T cell helper 1 (Th1) response ; Tropical viral diseases ; Viral diseases</subject><ispartof>Journal of medical virology, 2001-02, Vol.63 (2), p.150-157</ispartof><rights>Copyright © 2001 Wiley‐Liss, Inc.</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F1096-9071%2820000201%2963%3A2%3C150%3A%3AAID-JMV1010%3E3.0.CO%3B2-A$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F1096-9071%2820000201%2963%3A2%3C150%3A%3AAID-JMV1010%3E3.0.CO%3B2-A$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=892649$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Kuender D.</creatorcontrib><creatorcontrib>Yeh, Wen-Ting</creatorcontrib><creatorcontrib>Yang, Ming-Yu</creatorcontrib><creatorcontrib>Chen, Rong-Fu</creatorcontrib><creatorcontrib>Shaio, Men-Fang</creatorcontrib><title>Antibody-dependent enhancement of heterotypic dengue infections involved in suppression of IFNγ production</title><title>Journal of medical virology</title><addtitle>J. Med. Virol</addtitle><description>Antibody‐dependent enhancement has been implicated in some outbreaks of epidemic dengue hemorrhagic fever, however, the mechanism of antibody‐dependent enhancement is not well known. This study was conducted to investigate the cross‐protection and cross‐enhancement of dengue‐2 virus infections by dengue‐1 immune sera. It was found that dengue‐1 immune sera at 1:5 dilution (n = 12) could neutralize dengue‐2 infections in BHK‐21 cells, as assessed by a standard plaque‐reduction neutralization assay. Two‐thirds of the dengue‐1 immune sera at 1:25 dilution demonstrated neutralizing effects for dengue‐2 infections, whereas, non‐immune sera revealed no neutralization for dengue‐2 infections in BHK‐21 cells. Human mononuclear leukocytes in response to dengue‐2 infections elicited a T cell helper 1 (Th1) response revealing induction of IFNγ but not IL‐4 production. Dengue‐1 immune sera did not neutralize dengue‐2 infections in mononuclear leukocytes. Subneutralizing titers of dengue‐1 immune sera at 1:250, but not at 1:10 dilution, enhanced dengue‐2 infections in mononuclear leukocytes (1.2 ± 0.7 × 104 vs. 2.8 ± 0.3 × 102 PFU/ml). The enhancement of dengue‐2 infections in mononuclear leukocytes by dengue‐1 immune sera at 1:250 was associated with an increase in the lymphocyte proliferation index, and a decrease in IFNγ production (56 ± 24 vs. 12 ± 3 pg/ml). The addition of IFNγ (0.1 μg/ml) suppressed significantly the antibody‐dependent enhancement induced by dengue‐1 immune sera, whereas the presence of anti‐IFNγ F(ab)2 antibody augmented the antibody‐dependent enhancement effect. Results from this study suggest that suppression of Th1 response may be involved in the antibody‐dependent enhancement of heterotypic dengue infections. Better regulation of Th1/Th2 reactions may be useful for prevention of heterotypic immune enhancement of dengue infections. J. Med. Virol. 63:150–157, 2001. © 2001 Wiley‐Liss, Inc.</description><subject>antibody-dependent enhancement</subject><subject>Arboviroses</subject><subject>Biological and medical sciences</subject><subject>Dengue fevers</subject><subject>dengue hemorrhagic fever</subject><subject>Dengue virus 2</subject><subject>g-Interferon</subject><subject>Human viral diseases</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>plaque-forming units (PFU)</subject><subject>T cell helper 1 (Th1) response</subject><subject>Tropical viral diseases</subject><subject>Viral diseases</subject><issn>0146-6615</issn><issn>1096-9071</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNo9UdFu0zAUjSaQVsb-IRISgoeUe-3Gbjo0KSpsKyrrS8cQL1eJ7TCzNAlxMuh37T_2TTi0q198dc65x_I5QXCGMEYA9gEhEVECEt8x8IcBvhd8xj5iDLNZuvgUffn6DQHhnI9hPF-dsSg9CkaHrRfBCHAiIiEwPg5eOffLm0wTxkbBfVp1Nq_1NtKmMZU2VRea6i6rlNkMc12Ed6Yzbd1tG6tCz__sTWirwqjO1pXz40NdPhjth9D1TdMa5zwxLC4urp8ew6atdf9f_Dp4WWSlM6f7-yS4ufi8nl9Fy9XlYp4uI8s5QBTnkiPXTIHQTDIJhSzUFHLAnE1yzXKI8wQz9LCeaoRJjkoxZqTKFEqp-Enwdufrn_7dG9fRxjplyjKrTN07QjnlwBC88M1emDmVlUXrv20dNa3dZO2WfEJiknjV7U71x5Zme2ARaOiGhpRpSJmeuyHByeMxkK-G9tUQJ6D5yhPpM-Sdo52zdZ35e3DO2nsSksuYbq8vaXkl8Pt6_YMS_g_PVZpb</recordid><startdate>200102</startdate><enddate>200102</enddate><creator>Yang, Kuender D.</creator><creator>Yeh, Wen-Ting</creator><creator>Yang, Ming-Yu</creator><creator>Chen, Rong-Fu</creator><creator>Shaio, Men-Fang</creator><general>John Wiley & Sons, Inc</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>200102</creationdate><title>Antibody-dependent enhancement of heterotypic dengue infections involved in suppression of IFNγ production</title><author>Yang, Kuender D. ; Yeh, Wen-Ting ; Yang, Ming-Yu ; Chen, Rong-Fu ; Shaio, Men-Fang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i3300-5b7313d2c06d27270f7fc80b01b24bd2b05b91a1f7fd8d104b1cc22e7cac177c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>antibody-dependent enhancement</topic><topic>Arboviroses</topic><topic>Biological and medical sciences</topic><topic>Dengue fevers</topic><topic>dengue hemorrhagic fever</topic><topic>Dengue virus 2</topic><topic>g-Interferon</topic><topic>Human viral diseases</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>plaque-forming units (PFU)</topic><topic>T cell helper 1 (Th1) response</topic><topic>Tropical viral diseases</topic><topic>Viral diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Kuender D.</creatorcontrib><creatorcontrib>Yeh, Wen-Ting</creatorcontrib><creatorcontrib>Yang, Ming-Yu</creatorcontrib><creatorcontrib>Chen, Rong-Fu</creatorcontrib><creatorcontrib>Shaio, Men-Fang</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Journal of medical virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Kuender D.</au><au>Yeh, Wen-Ting</au><au>Yang, Ming-Yu</au><au>Chen, Rong-Fu</au><au>Shaio, Men-Fang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antibody-dependent enhancement of heterotypic dengue infections involved in suppression of IFNγ production</atitle><jtitle>Journal of medical virology</jtitle><addtitle>J. Med. Virol</addtitle><date>2001-02</date><risdate>2001</risdate><volume>63</volume><issue>2</issue><spage>150</spage><epage>157</epage><pages>150-157</pages><issn>0146-6615</issn><eissn>1096-9071</eissn><coden>JMVIDB</coden><abstract>Antibody‐dependent enhancement has been implicated in some outbreaks of epidemic dengue hemorrhagic fever, however, the mechanism of antibody‐dependent enhancement is not well known. This study was conducted to investigate the cross‐protection and cross‐enhancement of dengue‐2 virus infections by dengue‐1 immune sera. It was found that dengue‐1 immune sera at 1:5 dilution (n = 12) could neutralize dengue‐2 infections in BHK‐21 cells, as assessed by a standard plaque‐reduction neutralization assay. Two‐thirds of the dengue‐1 immune sera at 1:25 dilution demonstrated neutralizing effects for dengue‐2 infections, whereas, non‐immune sera revealed no neutralization for dengue‐2 infections in BHK‐21 cells. Human mononuclear leukocytes in response to dengue‐2 infections elicited a T cell helper 1 (Th1) response revealing induction of IFNγ but not IL‐4 production. Dengue‐1 immune sera did not neutralize dengue‐2 infections in mononuclear leukocytes. Subneutralizing titers of dengue‐1 immune sera at 1:250, but not at 1:10 dilution, enhanced dengue‐2 infections in mononuclear leukocytes (1.2 ± 0.7 × 104 vs. 2.8 ± 0.3 × 102 PFU/ml). The enhancement of dengue‐2 infections in mononuclear leukocytes by dengue‐1 immune sera at 1:250 was associated with an increase in the lymphocyte proliferation index, and a decrease in IFNγ production (56 ± 24 vs. 12 ± 3 pg/ml). The addition of IFNγ (0.1 μg/ml) suppressed significantly the antibody‐dependent enhancement induced by dengue‐1 immune sera, whereas the presence of anti‐IFNγ F(ab)2 antibody augmented the antibody‐dependent enhancement effect. Results from this study suggest that suppression of Th1 response may be involved in the antibody‐dependent enhancement of heterotypic dengue infections. Better regulation of Th1/Th2 reactions may be useful for prevention of heterotypic immune enhancement of dengue infections. J. Med. Virol. 63:150–157, 2001. © 2001 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>John Wiley & Sons, Inc</pub><doi>10.1002/1096-9071(20000201)63:2<150::AID-JMV1010>3.0.CO;2-A</doi><tpages>8</tpages></addata></record> |
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| subjects | antibody-dependent enhancement Arboviroses Biological and medical sciences Dengue fevers dengue hemorrhagic fever Dengue virus 2 g-Interferon Human viral diseases Infectious diseases Medical sciences plaque-forming units (PFU) T cell helper 1 (Th1) response Tropical viral diseases Viral diseases |
| title | Antibody-dependent enhancement of heterotypic dengue infections involved in suppression of IFNγ production |
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