A new transgene assay to study microsatellite instability in wild-type and mismatch-repair defective plant progenies
DNA mismatch repair (MMR) genes are essential for maintaining the fidelity of DNA replication in prokaryotes and eukaryotes. In most species, from Escherichia coli to human, alterations in this system result in elevated frequencies of microsatellite length alterations. In the present report, we desi...
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| Veröffentlicht in: | Plant science (Limerick) 2005-04, Vol.168 (4), p.939-947 |
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| creator | Depeiges, Annie Farget, Stephane Degroote, Fabienne Picard, Georges |
| description | DNA mismatch repair (MMR) genes are essential for maintaining the fidelity of DNA replication in prokaryotes and eukaryotes. In most species, from
Escherichia coli to human, alterations in this system result in elevated frequencies of microsatellite length alterations. In the present report, we designed an
in planta assay system to measure the frequencies of microsatellite instability in
Arabidopsis thaliana. A transgenic reporter line was selected in which a (CT)
23 dinucleotide disrupts the reading frame of a downstream protein (phosphinotricin acetyltransferase). Microsatellite variations that restore the reading frame can be scored as glufosinate-resistant seedlings in the progenies. The spontaneous frequency of reversion at the transgene in the transgenic line is about 4
×
10
−4 and is stable over at least four generations. We used this assay system to evaluate the effect of an alteration of the function of ATMSH2, the
Arabidopsis homologue of MSH2, on microsatellite stability. Homozygous plants of the
ATMSH2 insertion mutant line Salk_002708 show a 60-fold increase of the reversion frequency in their progenies. This effect was confirmed by RNAi inactivation of ATMSH2. The reporter line can thus be used either to perform functional analyses of genes putatively involved in MMR control or to evaluate the effect of biotic and abiotic stress on microsatellite stability in plant progenies. |
| doi_str_mv | 10.1016/j.plantsci.2004.11.006 |
| format | Article |
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Escherichia coli to human, alterations in this system result in elevated frequencies of microsatellite length alterations. In the present report, we designed an
in planta assay system to measure the frequencies of microsatellite instability in
Arabidopsis thaliana. A transgenic reporter line was selected in which a (CT)
23 dinucleotide disrupts the reading frame of a downstream protein (phosphinotricin acetyltransferase). Microsatellite variations that restore the reading frame can be scored as glufosinate-resistant seedlings in the progenies. The spontaneous frequency of reversion at the transgene in the transgenic line is about 4
×
10
−4 and is stable over at least four generations. We used this assay system to evaluate the effect of an alteration of the function of ATMSH2, the
Arabidopsis homologue of MSH2, on microsatellite stability. Homozygous plants of the
ATMSH2 insertion mutant line Salk_002708 show a 60-fold increase of the reversion frequency in their progenies. This effect was confirmed by RNAi inactivation of ATMSH2. The reporter line can thus be used either to perform functional analyses of genes putatively involved in MMR control or to evaluate the effect of biotic and abiotic stress on microsatellite stability in plant progenies.</description><identifier>ISSN: 0168-9452</identifier><identifier>EISSN: 1873-2259</identifier><identifier>DOI: 10.1016/j.plantsci.2004.11.006</identifier><identifier>CODEN: PLSCE4</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>A TMSH2 mutant ; Arabidopsis ; Arabidopsis thaliana ; base pair mismatch ; Biological and medical sciences ; DNA mismatch repair protein ; DNA repair ; Escherichia coli ; Fundamental and applied biological sciences. Psychology ; Genes. Genome ; Glufosinate resistance ; Microsatellite instability ; microsatellite repeats ; Mismatch repair ; Molecular and cellular biology ; Molecular genetics ; molecular sequence data ; nucleotide sequences ; plant proteins ; RNA interference ; RNAi ; transgenes</subject><ispartof>Plant science (Limerick), 2005-04, Vol.168 (4), p.939-947</ispartof><rights>2004 Elsevier Ireland Ltd</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c397t-4a898c4c6cbde22bbba823357afe0b018bc5339d26abb4afed0a4d6edf75167a3</citedby><cites>FETCH-LOGICAL-c397t-4a898c4c6cbde22bbba823357afe0b018bc5339d26abb4afed0a4d6edf75167a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.plantsci.2004.11.006$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16568769$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Depeiges, Annie</creatorcontrib><creatorcontrib>Farget, Stephane</creatorcontrib><creatorcontrib>Degroote, Fabienne</creatorcontrib><creatorcontrib>Picard, Georges</creatorcontrib><title>A new transgene assay to study microsatellite instability in wild-type and mismatch-repair defective plant progenies</title><title>Plant science (Limerick)</title><description>DNA mismatch repair (MMR) genes are essential for maintaining the fidelity of DNA replication in prokaryotes and eukaryotes. In most species, from
Escherichia coli to human, alterations in this system result in elevated frequencies of microsatellite length alterations. In the present report, we designed an
in planta assay system to measure the frequencies of microsatellite instability in
Arabidopsis thaliana. A transgenic reporter line was selected in which a (CT)
23 dinucleotide disrupts the reading frame of a downstream protein (phosphinotricin acetyltransferase). Microsatellite variations that restore the reading frame can be scored as glufosinate-resistant seedlings in the progenies. The spontaneous frequency of reversion at the transgene in the transgenic line is about 4
×
10
−4 and is stable over at least four generations. We used this assay system to evaluate the effect of an alteration of the function of ATMSH2, the
Arabidopsis homologue of MSH2, on microsatellite stability. Homozygous plants of the
ATMSH2 insertion mutant line Salk_002708 show a 60-fold increase of the reversion frequency in their progenies. This effect was confirmed by RNAi inactivation of ATMSH2. The reporter line can thus be used either to perform functional analyses of genes putatively involved in MMR control or to evaluate the effect of biotic and abiotic stress on microsatellite stability in plant progenies.</description><subject>A TMSH2 mutant</subject><subject>Arabidopsis</subject><subject>Arabidopsis thaliana</subject><subject>base pair mismatch</subject><subject>Biological and medical sciences</subject><subject>DNA mismatch repair protein</subject><subject>DNA repair</subject><subject>Escherichia coli</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes. Genome</subject><subject>Glufosinate resistance</subject><subject>Microsatellite instability</subject><subject>microsatellite repeats</subject><subject>Mismatch repair</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>molecular sequence data</subject><subject>nucleotide sequences</subject><subject>plant proteins</subject><subject>RNA interference</subject><subject>RNAi</subject><subject>transgenes</subject><issn>0168-9452</issn><issn>1873-2259</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqFkE1v1DAQhi0EEkvhL4AvcEuwncROblQVH5UqcYCerbE9KV5lk2B7W-XfM8sWcezJlvWM33cext5KUUsh9cd9vU4wl-xjrYRoaylrIfQztpO9aSqluuE52xHYV0PbqZfsVc57IYTqOrNj5ZLP-MBLgjnf4YwccoaNl4XncgwbP0SflgwFpykW5HHOBVyk-0Z3_hCnUJVtpbE5EJsPUPyvKuEKMfGAI_oS75H_LcjXtFBExPyavRhhyvjm8bxgt18-_7z6Vt18_3p9dXlT-WYwpWqhH3rfeu1dQKWcc9CrpukMjCickL3zXdMMQWlwrqXHIKANGsNoOqkNNBfsw_lfSv59xFwsVfS0Csy4HLOVpleDVoZAfQZPy-aEo11TPEDarBT2JNnu7T_J9iTZSmlJMg2-f0yA7GEaSaOP-f-07nRv9EDcuzM3wmLhLhFz-0MJ2QgxGNN2pwqfzgSSkPuIyVIWzh5DTGTRhiU-VeYPXMmjUg</recordid><startdate>20050401</startdate><enddate>20050401</enddate><creator>Depeiges, Annie</creator><creator>Farget, Stephane</creator><creator>Degroote, Fabienne</creator><creator>Picard, Georges</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20050401</creationdate><title>A new transgene assay to study microsatellite instability in wild-type and mismatch-repair defective plant progenies</title><author>Depeiges, Annie ; Farget, Stephane ; Degroote, Fabienne ; Picard, Georges</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c397t-4a898c4c6cbde22bbba823357afe0b018bc5339d26abb4afed0a4d6edf75167a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>A TMSH2 mutant</topic><topic>Arabidopsis</topic><topic>Arabidopsis thaliana</topic><topic>base pair mismatch</topic><topic>Biological and medical sciences</topic><topic>DNA mismatch repair protein</topic><topic>DNA repair</topic><topic>Escherichia coli</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes. Genome</topic><topic>Glufosinate resistance</topic><topic>Microsatellite instability</topic><topic>microsatellite repeats</topic><topic>Mismatch repair</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>molecular sequence data</topic><topic>nucleotide sequences</topic><topic>plant proteins</topic><topic>RNA interference</topic><topic>RNAi</topic><topic>transgenes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Depeiges, Annie</creatorcontrib><creatorcontrib>Farget, Stephane</creatorcontrib><creatorcontrib>Degroote, Fabienne</creatorcontrib><creatorcontrib>Picard, Georges</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Plant science (Limerick)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Depeiges, Annie</au><au>Farget, Stephane</au><au>Degroote, Fabienne</au><au>Picard, Georges</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A new transgene assay to study microsatellite instability in wild-type and mismatch-repair defective plant progenies</atitle><jtitle>Plant science (Limerick)</jtitle><date>2005-04-01</date><risdate>2005</risdate><volume>168</volume><issue>4</issue><spage>939</spage><epage>947</epage><pages>939-947</pages><issn>0168-9452</issn><eissn>1873-2259</eissn><coden>PLSCE4</coden><abstract>DNA mismatch repair (MMR) genes are essential for maintaining the fidelity of DNA replication in prokaryotes and eukaryotes. In most species, from
Escherichia coli to human, alterations in this system result in elevated frequencies of microsatellite length alterations. In the present report, we designed an
in planta assay system to measure the frequencies of microsatellite instability in
Arabidopsis thaliana. A transgenic reporter line was selected in which a (CT)
23 dinucleotide disrupts the reading frame of a downstream protein (phosphinotricin acetyltransferase). Microsatellite variations that restore the reading frame can be scored as glufosinate-resistant seedlings in the progenies. The spontaneous frequency of reversion at the transgene in the transgenic line is about 4
×
10
−4 and is stable over at least four generations. We used this assay system to evaluate the effect of an alteration of the function of ATMSH2, the
Arabidopsis homologue of MSH2, on microsatellite stability. Homozygous plants of the
ATMSH2 insertion mutant line Salk_002708 show a 60-fold increase of the reversion frequency in their progenies. This effect was confirmed by RNAi inactivation of ATMSH2. The reporter line can thus be used either to perform functional analyses of genes putatively involved in MMR control or to evaluate the effect of biotic and abiotic stress on microsatellite stability in plant progenies.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><doi>10.1016/j.plantsci.2004.11.006</doi><tpages>9</tpages></addata></record> |
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| source | Elsevier ScienceDirect Journals |
| subjects | A TMSH2 mutant Arabidopsis Arabidopsis thaliana base pair mismatch Biological and medical sciences DNA mismatch repair protein DNA repair Escherichia coli Fundamental and applied biological sciences. Psychology Genes. Genome Glufosinate resistance Microsatellite instability microsatellite repeats Mismatch repair Molecular and cellular biology Molecular genetics molecular sequence data nucleotide sequences plant proteins RNA interference RNAi transgenes |
| title | A new transgene assay to study microsatellite instability in wild-type and mismatch-repair defective plant progenies |
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