Effects of an aqueous extract of processed bidi tobacco on the growth of hamster tracheal epithelial cells
Inhalation of tobacco dust is responsible for elevated genotoxicity and pulmonary ailments in workers engaged in processing tobacco for the manufacture of bidis, the Indian version of cigarettes. Tracheal tissue being the major site of interaction with tobacco dust, the effects of different concentr...
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| Veröffentlicht in: | Toxicology letters 2001-02, Vol.119 (1), p.1-9 |
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| creator | Shah, Manish D Ramchandani, Asha G Mahimkar, Manoj B Potdar, Pravin D Bhisey, Avinash N Bhisey, Rajani A |
| description | Inhalation of tobacco dust is responsible for elevated genotoxicity and pulmonary ailments in workers engaged in processing tobacco for the manufacture of bidis, the Indian version of cigarettes. Tracheal tissue being the major site of interaction with tobacco dust, the effects of different concentrations of an aqueous extract of bidi tobacco (ATE) on the growth of a hamster tracheal epithelial cell line (HTE) were investigated. Colony forming efficiency assay revealed that ATE was cytotoxic only at the highest concentration of 5.0 mg/ml. In cultures treated with 1.25 mg/ml ATE, the cell doubling time and growth rate were similar to that of the controls, while a significant increase in cell doubling time (29.4±0.3 h vs 14.0±3.75 h,
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| doi_str_mv | 10.1016/S0378-4274(00)00275-7 |
| format | Article |
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P<0.001) was observed at 2.5 mg/ml ATE concentration. Exposure of HTE cells to the non-toxic ATE concentration of 2.5 mg/ml was found to stimulate ornithine decarboxylase (ODC) activity, incorporation of [
3H] methyl thymidine into DNA and increase in the S phase fraction was seen by flow cytometry. However, a 56% reduction in the growth rate of cultures treated with 2.5 mg/ml ATE was related to the prolongation of the traverse of cells through S phase. ATE-induced growth suppression was reversed when cultures were grown in ATE-free medium or upon repeated exposure to ATE. The findings suggest that increased tracheal cell proliferation induced by chronic inhalation of tobacco dust may contribute to the development of pulmonary disorders and possibly neoplasia in exposed individuals.</description><identifier>ISSN: 0378-4274</identifier><identifier>EISSN: 1879-3169</identifier><identifier>DOI: 10.1016/S0378-4274(00)00275-7</identifier><identifier>PMID: 11275416</identifier><identifier>CODEN: TOLED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Animals ; bidi ; Bidi tobacco ; Biological and medical sciences ; Cell Cycle - drug effects ; Cell Division - drug effects ; Cell Line ; Cricetinae ; DNA - biosynthesis ; Dust - adverse effects ; epithelial cells ; Epithelial Cells - pathology ; Flow Cytometry ; Growth kinetics ; Hamster tracheal epithelial cells ; Humans ; Medical sciences ; Mesocricetus ; Nicotiana - chemistry ; Nicotiana - toxicity ; Ornithine Decarboxylase - biosynthesis ; Plant Extracts - toxicity ; Plants, Toxic ; Scintillation Counting ; Thymidine - chemistry ; Tobacco, tobacco smoking ; Toxicology ; Trachea - drug effects ; Trachea - metabolism ; Trachea - pathology ; Tritium ; Water - chemistry</subject><ispartof>Toxicology letters, 2001-02, Vol.119 (1), p.1-9</ispartof><rights>2001 Elsevier Science Ireland Ltd</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-a24654b062c3a0cacf2f9af03741aba6984edaf9de646f6708ede36feb557b2d3</citedby><cites>FETCH-LOGICAL-c420t-a24654b062c3a0cacf2f9af03741aba6984edaf9de646f6708ede36feb557b2d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0378-4274(00)00275-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=878756$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11275416$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shah, Manish D</creatorcontrib><creatorcontrib>Ramchandani, Asha G</creatorcontrib><creatorcontrib>Mahimkar, Manoj B</creatorcontrib><creatorcontrib>Potdar, Pravin D</creatorcontrib><creatorcontrib>Bhisey, Avinash N</creatorcontrib><creatorcontrib>Bhisey, Rajani A</creatorcontrib><title>Effects of an aqueous extract of processed bidi tobacco on the growth of hamster tracheal epithelial cells</title><title>Toxicology letters</title><addtitle>Toxicol Lett</addtitle><description>Inhalation of tobacco dust is responsible for elevated genotoxicity and pulmonary ailments in workers engaged in processing tobacco for the manufacture of bidis, the Indian version of cigarettes. Tracheal tissue being the major site of interaction with tobacco dust, the effects of different concentrations of an aqueous extract of bidi tobacco (ATE) on the growth of a hamster tracheal epithelial cell line (HTE) were investigated. Colony forming efficiency assay revealed that ATE was cytotoxic only at the highest concentration of 5.0 mg/ml. In cultures treated with 1.25 mg/ml ATE, the cell doubling time and growth rate were similar to that of the controls, while a significant increase in cell doubling time (29.4±0.3 h vs 14.0±3.75 h,
P<0.001) was observed at 2.5 mg/ml ATE concentration. Exposure of HTE cells to the non-toxic ATE concentration of 2.5 mg/ml was found to stimulate ornithine decarboxylase (ODC) activity, incorporation of [
3H] methyl thymidine into DNA and increase in the S phase fraction was seen by flow cytometry. However, a 56% reduction in the growth rate of cultures treated with 2.5 mg/ml ATE was related to the prolongation of the traverse of cells through S phase. ATE-induced growth suppression was reversed when cultures were grown in ATE-free medium or upon repeated exposure to ATE. The findings suggest that increased tracheal cell proliferation induced by chronic inhalation of tobacco dust may contribute to the development of pulmonary disorders and possibly neoplasia in exposed individuals.</description><subject>Animals</subject><subject>bidi</subject><subject>Bidi tobacco</subject><subject>Biological and medical sciences</subject><subject>Cell Cycle - drug effects</subject><subject>Cell Division - drug effects</subject><subject>Cell Line</subject><subject>Cricetinae</subject><subject>DNA - biosynthesis</subject><subject>Dust - adverse effects</subject><subject>epithelial cells</subject><subject>Epithelial Cells - pathology</subject><subject>Flow Cytometry</subject><subject>Growth kinetics</subject><subject>Hamster tracheal epithelial cells</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Mesocricetus</subject><subject>Nicotiana - chemistry</subject><subject>Nicotiana - toxicity</subject><subject>Ornithine Decarboxylase - biosynthesis</subject><subject>Plant Extracts - toxicity</subject><subject>Plants, Toxic</subject><subject>Scintillation Counting</subject><subject>Thymidine - chemistry</subject><subject>Tobacco, tobacco smoking</subject><subject>Toxicology</subject><subject>Trachea - drug effects</subject><subject>Trachea - metabolism</subject><subject>Trachea - pathology</subject><subject>Tritium</subject><subject>Water - chemistry</subject><issn>0378-4274</issn><issn>1879-3169</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV9rFDEUxYModlv9CJaAUPRh9Gb-JDNPIqW1QsEH9TncSW66KbOTbZLV9tub6S7to3lJCL-Te84JY-8EfBIg5Oef0Ki-amvVfgD4CFCrrlIv2Er0aqgaIYeXbPWEHLHjlG4BQLaye82OhCh4K-SK3V44RyYnHhzHmePdjsIucbrPEU1ebrcxGEqJLB-99TyHEY0JPMw8r4nfxPA3rxdujZuUKfJFuCacOG19ISZfjoamKb1hrxxOid4e9hP2-_Li1_lVdf3j2_fzr9eVaWvIFdbFYzuCrE2DYNC42g3oSpZW4Ihy6Fuy6AZLJYyTCnqy1EhHY9epsbbNCTvbv1uclzgp641PiwOcl2xaqL4uSxaw24MmhpQiOb2NfoPxQQvQS8n6sWS9NKgB9GPJWhXd6WHAbtyQfVYdWi3A-wOAyeDkIs7GpyeuV73qFurLnqJSxh9PUSfjaTZkfSxfom3w_zHyD60tmiw</recordid><startdate>20010203</startdate><enddate>20010203</enddate><creator>Shah, Manish D</creator><creator>Ramchandani, Asha G</creator><creator>Mahimkar, Manoj B</creator><creator>Potdar, Pravin D</creator><creator>Bhisey, Avinash N</creator><creator>Bhisey, Rajani A</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20010203</creationdate><title>Effects of an aqueous extract of processed bidi tobacco on the growth of hamster tracheal epithelial cells</title><author>Shah, Manish D ; Ramchandani, Asha G ; Mahimkar, Manoj B ; Potdar, Pravin D ; Bhisey, Avinash N ; Bhisey, Rajani A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-a24654b062c3a0cacf2f9af03741aba6984edaf9de646f6708ede36feb557b2d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>bidi</topic><topic>Bidi tobacco</topic><topic>Biological and medical sciences</topic><topic>Cell Cycle - drug effects</topic><topic>Cell Division - drug effects</topic><topic>Cell Line</topic><topic>Cricetinae</topic><topic>DNA - biosynthesis</topic><topic>Dust - adverse effects</topic><topic>epithelial cells</topic><topic>Epithelial Cells - pathology</topic><topic>Flow Cytometry</topic><topic>Growth kinetics</topic><topic>Hamster tracheal epithelial cells</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Mesocricetus</topic><topic>Nicotiana - chemistry</topic><topic>Nicotiana - toxicity</topic><topic>Ornithine Decarboxylase - biosynthesis</topic><topic>Plant Extracts - toxicity</topic><topic>Plants, Toxic</topic><topic>Scintillation Counting</topic><topic>Thymidine - chemistry</topic><topic>Tobacco, tobacco smoking</topic><topic>Toxicology</topic><topic>Trachea - drug effects</topic><topic>Trachea - metabolism</topic><topic>Trachea - pathology</topic><topic>Tritium</topic><topic>Water - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shah, Manish D</creatorcontrib><creatorcontrib>Ramchandani, Asha G</creatorcontrib><creatorcontrib>Mahimkar, Manoj B</creatorcontrib><creatorcontrib>Potdar, Pravin D</creatorcontrib><creatorcontrib>Bhisey, Avinash N</creatorcontrib><creatorcontrib>Bhisey, Rajani A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Toxicology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shah, Manish D</au><au>Ramchandani, Asha G</au><au>Mahimkar, Manoj B</au><au>Potdar, Pravin D</au><au>Bhisey, Avinash N</au><au>Bhisey, Rajani A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of an aqueous extract of processed bidi tobacco on the growth of hamster tracheal epithelial cells</atitle><jtitle>Toxicology letters</jtitle><addtitle>Toxicol Lett</addtitle><date>2001-02-03</date><risdate>2001</risdate><volume>119</volume><issue>1</issue><spage>1</spage><epage>9</epage><pages>1-9</pages><issn>0378-4274</issn><eissn>1879-3169</eissn><coden>TOLED5</coden><abstract>Inhalation of tobacco dust is responsible for elevated genotoxicity and pulmonary ailments in workers engaged in processing tobacco for the manufacture of bidis, the Indian version of cigarettes. Tracheal tissue being the major site of interaction with tobacco dust, the effects of different concentrations of an aqueous extract of bidi tobacco (ATE) on the growth of a hamster tracheal epithelial cell line (HTE) were investigated. Colony forming efficiency assay revealed that ATE was cytotoxic only at the highest concentration of 5.0 mg/ml. In cultures treated with 1.25 mg/ml ATE, the cell doubling time and growth rate were similar to that of the controls, while a significant increase in cell doubling time (29.4±0.3 h vs 14.0±3.75 h,
P<0.001) was observed at 2.5 mg/ml ATE concentration. Exposure of HTE cells to the non-toxic ATE concentration of 2.5 mg/ml was found to stimulate ornithine decarboxylase (ODC) activity, incorporation of [
3H] methyl thymidine into DNA and increase in the S phase fraction was seen by flow cytometry. However, a 56% reduction in the growth rate of cultures treated with 2.5 mg/ml ATE was related to the prolongation of the traverse of cells through S phase. ATE-induced growth suppression was reversed when cultures were grown in ATE-free medium or upon repeated exposure to ATE. The findings suggest that increased tracheal cell proliferation induced by chronic inhalation of tobacco dust may contribute to the development of pulmonary disorders and possibly neoplasia in exposed individuals.</abstract><cop>Shannon</cop><cop>Amsterdam</cop><pub>Elsevier Ireland Ltd</pub><pmid>11275416</pmid><doi>10.1016/S0378-4274(00)00275-7</doi><tpages>9</tpages></addata></record> |
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| subjects | Animals bidi Bidi tobacco Biological and medical sciences Cell Cycle - drug effects Cell Division - drug effects Cell Line Cricetinae DNA - biosynthesis Dust - adverse effects epithelial cells Epithelial Cells - pathology Flow Cytometry Growth kinetics Hamster tracheal epithelial cells Humans Medical sciences Mesocricetus Nicotiana - chemistry Nicotiana - toxicity Ornithine Decarboxylase - biosynthesis Plant Extracts - toxicity Plants, Toxic Scintillation Counting Thymidine - chemistry Tobacco, tobacco smoking Toxicology Trachea - drug effects Trachea - metabolism Trachea - pathology Tritium Water - chemistry |
| title | Effects of an aqueous extract of processed bidi tobacco on the growth of hamster tracheal epithelial cells |
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