Determination of macrolide antibiotics in meat and fish by liquid chromatography–electrospray mass spectrometry
A simple and reliable method using liquid chromatography–electrospray ionization-mass spectrometry (LC–ESI-MS) has been developed for the determination of macrolide antibiotics, erythromycin (EM), oleandomycin (OM), kitasamycin (KT), josamycin (JM), mirosamicin (MRM), spiramycin (SPM), tilmicosin (T...
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| Veröffentlicht in: | Analytica chimica acta 2003-09, Vol.492 (1), p.187-197 |
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| creator | Horie, Masakazu Takegami, Harumi Toya, Kazuo Nakazawa, Hiroyuki |
| description | A simple and reliable method using liquid chromatography–electrospray ionization-mass spectrometry (LC–ESI-MS) has been developed for the determination of macrolide antibiotics, erythromycin (EM), oleandomycin (OM), kitasamycin (KT), josamycin (JM), mirosamicin (MRM), spiramycin (SPM), tilmicosin (TLM) and tylosin (TS) in meat. The LC separation was performed on a TSKgel Super ODS column (
100
mm×2
mm i.d.) with a gradient system of 0.2% acetic acid–acetonitrile (containing 0.2% acetic acid) as the mobile phase at the flow rate of 0.2
ml/min. The positive ionization produced the molecular related ions, (M+2H)
2+, at
m/
z 422 and 435 for SPM and TLM, and (M+H)
+, at 734, 688, 772, 828, 728 and 916 for EM, OM, KT, JM, MRM and TS, respectively. The calibration graphs for each drug were rectilinear from 0.05 to 25
ng with selected ion monitoring (SIM). The drugs were extracted with 0.2% metaphosphoric acid–methanol (6:4), and the extracts were cleaned up on an Oasis HLB cartridge (60
mg). The recoveries of the drugs from meat and fish fortified at the 0.2
μg/g level was 70.4–93.2% with high precision. The limits of quantification of the drugs in meat and fish were 0.01
μg/g. |
| doi_str_mv | 10.1016/S0003-2670(03)00891-2 |
| format | Article |
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100
mm×2
mm i.d.) with a gradient system of 0.2% acetic acid–acetonitrile (containing 0.2% acetic acid) as the mobile phase at the flow rate of 0.2
ml/min. The positive ionization produced the molecular related ions, (M+2H)
2+, at
m/
z 422 and 435 for SPM and TLM, and (M+H)
+, at 734, 688, 772, 828, 728 and 916 for EM, OM, KT, JM, MRM and TS, respectively. The calibration graphs for each drug were rectilinear from 0.05 to 25
ng with selected ion monitoring (SIM). The drugs were extracted with 0.2% metaphosphoric acid–methanol (6:4), and the extracts were cleaned up on an Oasis HLB cartridge (60
mg). The recoveries of the drugs from meat and fish fortified at the 0.2
μg/g level was 70.4–93.2% with high precision. The limits of quantification of the drugs in meat and fish were 0.01
μg/g.</description><identifier>ISSN: 0003-2670</identifier><identifier>EISSN: 1873-4324</identifier><identifier>DOI: 10.1016/S0003-2670(03)00891-2</identifier><identifier>CODEN: ACACAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Analytical chemistry ; Biological and medical sciences ; Chemistry ; Chromatographic methods and physical methods associated with chromatography ; Erythromycin ; Exact sciences and technology ; Fish ; General pharmacology ; Josamycin ; Kitasamycin ; LC–MS ; Macrolide antibiotics ; Mass spectrometry ; Meat ; Medical sciences ; Mirosamicin ; Oleandomycin ; Other chromatographic methods ; Pharmacology. Drug treatments ; Spectrometric and optical methods ; Spiramycin ; Tilmicosin ; Tylosin</subject><ispartof>Analytica chimica acta, 2003-09, Vol.492 (1), p.187-197</ispartof><rights>2003 Elsevier B.V.</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c465t-11c7bd4e9c2a98b6f3b2d7d3c79ca9c06d8c9b8de552c29bdc600dce489cced3</citedby><cites>FETCH-LOGICAL-c465t-11c7bd4e9c2a98b6f3b2d7d3c79ca9c06d8c9b8de552c29bdc600dce489cced3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0003267003008912$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15102066$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Horie, Masakazu</creatorcontrib><creatorcontrib>Takegami, Harumi</creatorcontrib><creatorcontrib>Toya, Kazuo</creatorcontrib><creatorcontrib>Nakazawa, Hiroyuki</creatorcontrib><title>Determination of macrolide antibiotics in meat and fish by liquid chromatography–electrospray mass spectrometry</title><title>Analytica chimica acta</title><description>A simple and reliable method using liquid chromatography–electrospray ionization-mass spectrometry (LC–ESI-MS) has been developed for the determination of macrolide antibiotics, erythromycin (EM), oleandomycin (OM), kitasamycin (KT), josamycin (JM), mirosamicin (MRM), spiramycin (SPM), tilmicosin (TLM) and tylosin (TS) in meat. The LC separation was performed on a TSKgel Super ODS column (
100
mm×2
mm i.d.) with a gradient system of 0.2% acetic acid–acetonitrile (containing 0.2% acetic acid) as the mobile phase at the flow rate of 0.2
ml/min. The positive ionization produced the molecular related ions, (M+2H)
2+, at
m/
z 422 and 435 for SPM and TLM, and (M+H)
+, at 734, 688, 772, 828, 728 and 916 for EM, OM, KT, JM, MRM and TS, respectively. The calibration graphs for each drug were rectilinear from 0.05 to 25
ng with selected ion monitoring (SIM). The drugs were extracted with 0.2% metaphosphoric acid–methanol (6:4), and the extracts were cleaned up on an Oasis HLB cartridge (60
mg). The recoveries of the drugs from meat and fish fortified at the 0.2
μg/g level was 70.4–93.2% with high precision. The limits of quantification of the drugs in meat and fish were 0.01
μg/g.</description><subject>Analysis</subject><subject>Analytical chemistry</subject><subject>Biological and medical sciences</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Erythromycin</subject><subject>Exact sciences and technology</subject><subject>Fish</subject><subject>General pharmacology</subject><subject>Josamycin</subject><subject>Kitasamycin</subject><subject>LC–MS</subject><subject>Macrolide antibiotics</subject><subject>Mass spectrometry</subject><subject>Meat</subject><subject>Medical sciences</subject><subject>Mirosamicin</subject><subject>Oleandomycin</subject><subject>Other chromatographic methods</subject><subject>Pharmacology. Drug treatments</subject><subject>Spectrometric and optical methods</subject><subject>Spiramycin</subject><subject>Tilmicosin</subject><subject>Tylosin</subject><issn>0003-2670</issn><issn>1873-4324</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNqNkc9q3DAQh0VoIdu0j1DQJaE5uB3J_0-lJE0aCOSQ3IU8GndVbMsraQO-5R3yhn2SandDe2xOg4ZvZtD3Y-yjgM8CRPXlHgDyTFY1fIL8HKBpRSaP2Eo0dZ4VuSzesNVf5Ji9C-FXekoBxYptLimSH-2ko3UTdz0fNXo3WENcT9F21kWLgduJj6Rj6hne27Dm3cIHu9law3Ht3aij--n1vF5-Pz3TQBi9C7PXS1oXAg_zvjNS9Mt79rbXQ6APL_WEPVx9f7j4kd3eXd9cfLvNsKjKmAmBdWcKalHqtumqPu-kqU2OdYu6RahMg23XGCpLibLtDFYABqloWkQy-Qk7O6ydvdtsKUQ12oA0DHoitw1K1I0EUZevAGUjk8UElgcw-QnBU69mb0ftFyVA7YJQ-yDUzrJKdR-E2s2dvhzQAfXQez2hDf-GSwESqipxXw8cJSuPlrwKaGlKn7E-6VPG2f9c-gNoU6Fl</recordid><startdate>20030901</startdate><enddate>20030901</enddate><creator>Horie, Masakazu</creator><creator>Takegami, Harumi</creator><creator>Toya, Kazuo</creator><creator>Nakazawa, Hiroyuki</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>C1K</scope><scope>F1W</scope><scope>H97</scope><scope>L.G</scope><scope>H95</scope></search><sort><creationdate>20030901</creationdate><title>Determination of macrolide antibiotics in meat and fish by liquid chromatography–electrospray mass spectrometry</title><author>Horie, Masakazu ; Takegami, Harumi ; Toya, Kazuo ; Nakazawa, Hiroyuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c465t-11c7bd4e9c2a98b6f3b2d7d3c79ca9c06d8c9b8de552c29bdc600dce489cced3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Analysis</topic><topic>Analytical chemistry</topic><topic>Biological and medical sciences</topic><topic>Chemistry</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Erythromycin</topic><topic>Exact sciences and technology</topic><topic>Fish</topic><topic>General pharmacology</topic><topic>Josamycin</topic><topic>Kitasamycin</topic><topic>LC–MS</topic><topic>Macrolide antibiotics</topic><topic>Mass spectrometry</topic><topic>Meat</topic><topic>Medical sciences</topic><topic>Mirosamicin</topic><topic>Oleandomycin</topic><topic>Other chromatographic methods</topic><topic>Pharmacology. Drug treatments</topic><topic>Spectrometric and optical methods</topic><topic>Spiramycin</topic><topic>Tilmicosin</topic><topic>Tylosin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Horie, Masakazu</creatorcontrib><creatorcontrib>Takegami, Harumi</creatorcontrib><creatorcontrib>Toya, Kazuo</creatorcontrib><creatorcontrib>Nakazawa, Hiroyuki</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><jtitle>Analytica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Horie, Masakazu</au><au>Takegami, Harumi</au><au>Toya, Kazuo</au><au>Nakazawa, Hiroyuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of macrolide antibiotics in meat and fish by liquid chromatography–electrospray mass spectrometry</atitle><jtitle>Analytica chimica acta</jtitle><date>2003-09-01</date><risdate>2003</risdate><volume>492</volume><issue>1</issue><spage>187</spage><epage>197</epage><pages>187-197</pages><issn>0003-2670</issn><eissn>1873-4324</eissn><coden>ACACAM</coden><abstract>A simple and reliable method using liquid chromatography–electrospray ionization-mass spectrometry (LC–ESI-MS) has been developed for the determination of macrolide antibiotics, erythromycin (EM), oleandomycin (OM), kitasamycin (KT), josamycin (JM), mirosamicin (MRM), spiramycin (SPM), tilmicosin (TLM) and tylosin (TS) in meat. The LC separation was performed on a TSKgel Super ODS column (
100
mm×2
mm i.d.) with a gradient system of 0.2% acetic acid–acetonitrile (containing 0.2% acetic acid) as the mobile phase at the flow rate of 0.2
ml/min. The positive ionization produced the molecular related ions, (M+2H)
2+, at
m/
z 422 and 435 for SPM and TLM, and (M+H)
+, at 734, 688, 772, 828, 728 and 916 for EM, OM, KT, JM, MRM and TS, respectively. The calibration graphs for each drug were rectilinear from 0.05 to 25
ng with selected ion monitoring (SIM). The drugs were extracted with 0.2% metaphosphoric acid–methanol (6:4), and the extracts were cleaned up on an Oasis HLB cartridge (60
mg). The recoveries of the drugs from meat and fish fortified at the 0.2
μg/g level was 70.4–93.2% with high precision. The limits of quantification of the drugs in meat and fish were 0.01
μg/g.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/S0003-2670(03)00891-2</doi><tpages>11</tpages></addata></record> |
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| subjects | Analysis Analytical chemistry Biological and medical sciences Chemistry Chromatographic methods and physical methods associated with chromatography Erythromycin Exact sciences and technology Fish General pharmacology Josamycin Kitasamycin LC–MS Macrolide antibiotics Mass spectrometry Meat Medical sciences Mirosamicin Oleandomycin Other chromatographic methods Pharmacology. Drug treatments Spectrometric and optical methods Spiramycin Tilmicosin Tylosin |
| title | Determination of macrolide antibiotics in meat and fish by liquid chromatography–electrospray mass spectrometry |
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