In vitro Crown Galls Induced by Agrobacterium tumefaciens Strain A281 (pTiBo542) in Trigonella foenum-graecum

In vitro Crown Galls Induced by Agrobacterium tumefaciens Strain A281 (pTiBo542) in Trigonella foenum-graecum

Transformation of fenugreek (Trigonella foenumgraecum) was carried out with A281 oncogenic strain of Agrobacterium tumefaciens using root, cotyledon and hypocotyl explants excised from 1-week-old seedlings, which showed that the plant was highly susceptible to transformation. Tumors (calli) were sel...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Biologia plantarum 2004-01, Vol.48 (3), p.441-444
Hauptverfasser: Khawar, K.M., Gulbitti-Onarici, S., Çöçü, S., Erisen, S., Sancak, C., Özcan, S.
Format: Artikel
Sprache:eng
Schlagworte:
Agrobacterium tumefaciens
Trigonella
Trigonella foenum-graecum
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Transformation of fenugreek (Trigonella foenumgraecum) was carried out with A281 oncogenic strain of Agrobacterium tumefaciens using root, cotyledon and hypocotyl explants excised from 1-week-old seedlings, which showed that the plant was highly susceptible to transformation. Tumors (calli) were selected on 50 mg dm super(-3) kanamycin. They were analyzed for beta -glucuronidase (GUS) expression. Presence of uidA (gus) gene, was confirmed by polymerase chain reaction (PCR) amplification.
ISSN:0006-3134
1573-8264
DOI:10.1023/B:BIOP.0000041100.94688.2d