Benzothiadiazole enhances the elicitation of rosmarinic acid production in a suspension culture of Agastache rugosa O. Kuntze
Callus and suspension cultures were established from the leaves of Agastache rugosa. The suspension cell growth was maximum at 15 days after inoculation. The cellular content of rosmarinic acid increased slowly and reached maximum (0.42 mg g super(-1) dry wt) during the stationary phase of culture,...
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| Veröffentlicht in: | Biotechnology letters 2001, Vol.23 (1), p.55-60 |
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| container_title | Biotechnology letters |
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| creator | HYE KYONG KIM OH, Sei-Ryang LEE, Hyeong-Kyu HUH, Hoon |
| description | Callus and suspension cultures were established from the leaves of Agastache rugosa. The suspension cell growth was maximum at 15 days after inoculation. The cellular content of rosmarinic acid increased slowly and reached maximum (0.42 mg g super(-1) dry wt) during the stationary phase of culture, after 18 days of inoculation. The addition of yeast extract preparation (MW < 10 000) at 50 mu g ml super(-1) elevated the rosmarinic acid content up to 5.7-fold of that found in non-elicited suspension cells. The elicitation of yeast extract preparation was further 2-fold enhanced by the presence of benzothiadiazole, a synthetic activator of plant systemic acquired resistance, as compared to yeast extract alone. These results showed that benzothiadiazole can be used as a tool for enhancing secondary metabolite accumulation in cell cultures. |
| doi_str_mv | 10.1023/A:1026738409671 |
| format | Article |
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These results showed that benzothiadiazole can be used as a tool for enhancing secondary metabolite accumulation in cell cultures.</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1023/A:1026738409671</identifier><identifier>CODEN: BILED3</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Agastache rugosa ; benzothiadiazole ; Biological and medical sciences ; Biotechnology ; Fundamental and applied biological sciences. Psychology ; Health. Pharmaceutical industry ; Industrial applications and implications. 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Kuntze</title><title>Biotechnology letters</title><description>Callus and suspension cultures were established from the leaves of Agastache rugosa. The suspension cell growth was maximum at 15 days after inoculation. The cellular content of rosmarinic acid increased slowly and reached maximum (0.42 mg g super(-1) dry wt) during the stationary phase of culture, after 18 days of inoculation. The addition of yeast extract preparation (MW < 10 000) at 50 mu g ml super(-1) elevated the rosmarinic acid content up to 5.7-fold of that found in non-elicited suspension cells. The elicitation of yeast extract preparation was further 2-fold enhanced by the presence of benzothiadiazole, a synthetic activator of plant systemic acquired resistance, as compared to yeast extract alone. These results showed that benzothiadiazole can be used as a tool for enhancing secondary metabolite accumulation in cell cultures.</description><subject>Agastache rugosa</subject><subject>benzothiadiazole</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Health. Pharmaceutical industry</subject><subject>Industrial applications and implications. 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Kuntze</title><author>HYE KYONG KIM ; OH, Sei-Ryang ; LEE, Hyeong-Kyu ; HUH, Hoon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c296t-99a6f888a7e85abb841584d7a5a12a7d1a6754f08d429137f4d3fcf4e0ef8bd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Agastache rugosa</topic><topic>benzothiadiazole</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Health. Pharmaceutical industry</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Other active biomolecules</topic><topic>Production of active biomolecules</topic><topic>rosmarinic acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HYE KYONG KIM</creatorcontrib><creatorcontrib>OH, Sei-Ryang</creatorcontrib><creatorcontrib>LEE, Hyeong-Kyu</creatorcontrib><creatorcontrib>HUH, Hoon</creatorcontrib><collection>Pascal-Francis</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biotechnology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HYE KYONG KIM</au><au>OH, Sei-Ryang</au><au>LEE, Hyeong-Kyu</au><au>HUH, Hoon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Benzothiadiazole enhances the elicitation of rosmarinic acid production in a suspension culture of Agastache rugosa O. Kuntze</atitle><jtitle>Biotechnology letters</jtitle><date>2001</date><risdate>2001</risdate><volume>23</volume><issue>1</issue><spage>55</spage><epage>60</epage><pages>55-60</pages><issn>0141-5492</issn><eissn>1573-6776</eissn><coden>BILED3</coden><abstract>Callus and suspension cultures were established from the leaves of Agastache rugosa. The suspension cell growth was maximum at 15 days after inoculation. The cellular content of rosmarinic acid increased slowly and reached maximum (0.42 mg g super(-1) dry wt) during the stationary phase of culture, after 18 days of inoculation. The addition of yeast extract preparation (MW < 10 000) at 50 mu g ml super(-1) elevated the rosmarinic acid content up to 5.7-fold of that found in non-elicited suspension cells. The elicitation of yeast extract preparation was further 2-fold enhanced by the presence of benzothiadiazole, a synthetic activator of plant systemic acquired resistance, as compared to yeast extract alone. These results showed that benzothiadiazole can be used as a tool for enhancing secondary metabolite accumulation in cell cultures.</abstract><cop>Dordrecht</cop><pub>Springer</pub><doi>10.1023/A:1026738409671</doi><tpages>6</tpages></addata></record> |
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| subjects | Agastache rugosa benzothiadiazole Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology Health. Pharmaceutical industry Industrial applications and implications. Economical aspects Other active biomolecules Production of active biomolecules rosmarinic acid |
| title | Benzothiadiazole enhances the elicitation of rosmarinic acid production in a suspension culture of Agastache rugosa O. Kuntze |
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