Oocyte aging in a marine protostome worm: The roles of maturation‐promoting factor and extracellular signal regulated kinase form of mitogen‐activated protein kinase
The roles of maturation‐promoting factor (MPF) and an extracellular signal regulated kinase form of mitogen‐activated protein kinase (ERK MAPK) are analyzed during oocyte aging in the marine protostome worm Cerebratulus. About a day after removal from the ovary, unfertilized metaphase‐I‐arrested ooc...
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| Veröffentlicht in: | Development, growth & differentiation growth & differentiation, 2016-04, Vol.58 (3), p.250-259 |
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| creator | Stricker, Stephen A. Beckstrom, Bradley Mendoza, Cristina Stanislawski, Emma Wodajo, Tewodros |
| description | The roles of maturation‐promoting factor (MPF) and an extracellular signal regulated kinase form of mitogen‐activated protein kinase (ERK MAPK) are analyzed during oocyte aging in the marine protostome worm Cerebratulus. About a day after removal from the ovary, unfertilized metaphase‐I‐arrested oocytes of Cerebratulus begin to flatten and swell before eventually lysing, thereby exhibiting characteristics of a necroptotic mode of regulated cell death. Based on immunoblots probed with phospho‐specific antibodies, MPF and ERK are initially active in freshly mature specimens. However, as oocytes age, both kinase activities decline, with ERK deactivation occurring well before MPF downregulation. Experiments using pharmacological modulators indicate that oocyte degradation is promoted by the maturation‐initiated activation of ERK as well as by the deactivation of MPF that occurs in extensively aged specimens. The potential significance of these findings is discussed relative to previously published results for apoptotic eggs and oocytes of echinoderm and vertebrate deuterostomes.
To supplement studies of other oocytes, morphological patterns of extraovarian aging are described for metaphase‐I‐arrested oocytes produced by Cerebratulus, an externally fertilizing marine worm of the phylum Nemertea. In addition, pharmacological modulators are combined with immunoblotting assays to provide data for this protostome that can be compared with previous reports for echinoderm and vertebrate deuterostomes. |
| doi_str_mv | 10.1111/dgd.12269 |
| format | Article |
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To supplement studies of other oocytes, morphological patterns of extraovarian aging are described for metaphase‐I‐arrested oocytes produced by Cerebratulus, an externally fertilizing marine worm of the phylum Nemertea. In addition, pharmacological modulators are combined with immunoblotting assays to provide data for this protostome that can be compared with previous reports for echinoderm and vertebrate deuterostomes.</description><identifier>ISSN: 0012-1592</identifier><identifier>EISSN: 1440-169X</identifier><identifier>DOI: 10.1111/dgd.12269</identifier><identifier>PMID: 26918273</identifier><identifier>CODEN: DGDFA5</identifier><language>eng</language><publisher>Japan: Wiley Subscription Services, Inc</publisher><subject>Animals ; apoptosis ; Apoptosis - physiology ; Butadienes - pharmacology ; Cells, Cultured ; Cellular Senescence - drug effects ; Cellular Senescence - physiology ; Cerebratulus ; Colforsin - pharmacology ; Extracellular Signal-Regulated MAP Kinases - antagonists & inhibitors ; Extracellular Signal-Regulated MAP Kinases - metabolism ; Female ; Immunoblotting ; Invertebrates - cytology ; Invertebrates - drug effects ; Invertebrates - metabolism ; Kinases ; Maturation-Promoting Factor - antagonists & inhibitors ; Maturation-Promoting Factor - metabolism ; necroptosis ; nemertean ; Nitriles - pharmacology ; non‐apoptotic regulated cell death ; Oocytes - cytology ; Oocytes - drug effects ; Oocytes - physiology ; Protein Kinase Inhibitors - pharmacology ; Purines - pharmacology ; Seawater - parasitology ; Time Factors ; Vasodilator Agents</subject><ispartof>Development, growth & differentiation, 2016-04, Vol.58 (3), p.250-259</ispartof><rights>2016 Japanese Society of Developmental Biologists</rights><rights>2016 Japanese Society of Developmental Biologists.</rights><rights>Copyright © 2016 Japanese Society of Developmental Biologists</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3889-57d1ce2b74c8bbb751b5d6af03d342947621d71072670c0e6680c4099595193d3</citedby><cites>FETCH-LOGICAL-c3889-57d1ce2b74c8bbb751b5d6af03d342947621d71072670c0e6680c4099595193d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fdgd.12269$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fdgd.12269$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26918273$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stricker, Stephen A.</creatorcontrib><creatorcontrib>Beckstrom, Bradley</creatorcontrib><creatorcontrib>Mendoza, Cristina</creatorcontrib><creatorcontrib>Stanislawski, Emma</creatorcontrib><creatorcontrib>Wodajo, Tewodros</creatorcontrib><title>Oocyte aging in a marine protostome worm: The roles of maturation‐promoting factor and extracellular signal regulated kinase form of mitogen‐activated protein kinase</title><title>Development, growth & differentiation</title><addtitle>Dev Growth Differ</addtitle><description>The roles of maturation‐promoting factor (MPF) and an extracellular signal regulated kinase form of mitogen‐activated protein kinase (ERK MAPK) are analyzed during oocyte aging in the marine protostome worm Cerebratulus. About a day after removal from the ovary, unfertilized metaphase‐I‐arrested oocytes of Cerebratulus begin to flatten and swell before eventually lysing, thereby exhibiting characteristics of a necroptotic mode of regulated cell death. Based on immunoblots probed with phospho‐specific antibodies, MPF and ERK are initially active in freshly mature specimens. However, as oocytes age, both kinase activities decline, with ERK deactivation occurring well before MPF downregulation. Experiments using pharmacological modulators indicate that oocyte degradation is promoted by the maturation‐initiated activation of ERK as well as by the deactivation of MPF that occurs in extensively aged specimens. The potential significance of these findings is discussed relative to previously published results for apoptotic eggs and oocytes of echinoderm and vertebrate deuterostomes.
To supplement studies of other oocytes, morphological patterns of extraovarian aging are described for metaphase‐I‐arrested oocytes produced by Cerebratulus, an externally fertilizing marine worm of the phylum Nemertea. In addition, pharmacological modulators are combined with immunoblotting assays to provide data for this protostome that can be compared with previous reports for echinoderm and vertebrate deuterostomes.</description><subject>Animals</subject><subject>apoptosis</subject><subject>Apoptosis - physiology</subject><subject>Butadienes - pharmacology</subject><subject>Cells, Cultured</subject><subject>Cellular Senescence - drug effects</subject><subject>Cellular Senescence - physiology</subject><subject>Cerebratulus</subject><subject>Colforsin - pharmacology</subject><subject>Extracellular Signal-Regulated MAP Kinases - antagonists & inhibitors</subject><subject>Extracellular Signal-Regulated MAP Kinases - metabolism</subject><subject>Female</subject><subject>Immunoblotting</subject><subject>Invertebrates - cytology</subject><subject>Invertebrates - drug effects</subject><subject>Invertebrates - metabolism</subject><subject>Kinases</subject><subject>Maturation-Promoting Factor - antagonists & inhibitors</subject><subject>Maturation-Promoting Factor - metabolism</subject><subject>necroptosis</subject><subject>nemertean</subject><subject>Nitriles - pharmacology</subject><subject>non‐apoptotic regulated cell death</subject><subject>Oocytes - cytology</subject><subject>Oocytes - drug effects</subject><subject>Oocytes - physiology</subject><subject>Protein Kinase Inhibitors - pharmacology</subject><subject>Purines - pharmacology</subject><subject>Seawater - parasitology</subject><subject>Time Factors</subject><subject>Vasodilator Agents</subject><issn>0012-1592</issn><issn>1440-169X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10U1u1DAUwHELgehQWHABZIkNLNL6eRw7ZofaUpAqdVMkdpHjvASXJC62Q5ldj8A1uBYnwZkUFkhkY0X66e-PR8hzYEeQv-O2b4-Ac6kfkA0IwQqQ-tNDsmEMeAGl5gfkSYzXjDEhgD8mB5lCxdV2Q35eertLSE3vpp66iRo6muAmpDfBJx-TH5He-jC-oVefkQY_YKS-yyjNwSTnp193PzIdfVoCnbHJB2qmluL3FIzFYZgHE2h0_WQGGrDPvwlb-sVNJiLtcnrfc8n3uMRywX3bk-UEmI-00qfkUWeGiM_u10Py8d3Z1cn74uLy_MPJ24vCbqtKF6VqwSJvlLBV0zSqhKZspenYtt0KroWSHFoFTHGpmGUoZcWsYFqXugSd0SF5tXbz9l9njKkeXVzuYSb0c6xBVQAlSCYzffkPvfZzyPfcKyYU5yXL6vWqbPAxBuzqm-DyI-9qYPUyvzrPr97PL9sX98W5GbH9K_8MLIPjFdy6AXf_L9Wn56dr8jcPgqiA</recordid><startdate>201604</startdate><enddate>201604</enddate><creator>Stricker, Stephen A.</creator><creator>Beckstrom, Bradley</creator><creator>Mendoza, Cristina</creator><creator>Stanislawski, Emma</creator><creator>Wodajo, Tewodros</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201604</creationdate><title>Oocyte aging in a marine protostome worm: The roles of maturation‐promoting factor and extracellular signal regulated kinase form of mitogen‐activated protein kinase</title><author>Stricker, Stephen A. ; Beckstrom, Bradley ; Mendoza, Cristina ; Stanislawski, Emma ; Wodajo, Tewodros</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3889-57d1ce2b74c8bbb751b5d6af03d342947621d71072670c0e6680c4099595193d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>apoptosis</topic><topic>Apoptosis - physiology</topic><topic>Butadienes - pharmacology</topic><topic>Cells, Cultured</topic><topic>Cellular Senescence - drug effects</topic><topic>Cellular Senescence - physiology</topic><topic>Cerebratulus</topic><topic>Colforsin - pharmacology</topic><topic>Extracellular Signal-Regulated MAP Kinases - antagonists & inhibitors</topic><topic>Extracellular Signal-Regulated MAP Kinases - metabolism</topic><topic>Female</topic><topic>Immunoblotting</topic><topic>Invertebrates - cytology</topic><topic>Invertebrates - drug effects</topic><topic>Invertebrates - metabolism</topic><topic>Kinases</topic><topic>Maturation-Promoting Factor - antagonists & inhibitors</topic><topic>Maturation-Promoting Factor - metabolism</topic><topic>necroptosis</topic><topic>nemertean</topic><topic>Nitriles - pharmacology</topic><topic>non‐apoptotic regulated cell death</topic><topic>Oocytes - cytology</topic><topic>Oocytes - drug effects</topic><topic>Oocytes - physiology</topic><topic>Protein Kinase Inhibitors - pharmacology</topic><topic>Purines - pharmacology</topic><topic>Seawater - parasitology</topic><topic>Time Factors</topic><topic>Vasodilator Agents</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stricker, Stephen A.</creatorcontrib><creatorcontrib>Beckstrom, Bradley</creatorcontrib><creatorcontrib>Mendoza, Cristina</creatorcontrib><creatorcontrib>Stanislawski, Emma</creatorcontrib><creatorcontrib>Wodajo, Tewodros</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Development, growth & differentiation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stricker, Stephen A.</au><au>Beckstrom, Bradley</au><au>Mendoza, Cristina</au><au>Stanislawski, Emma</au><au>Wodajo, Tewodros</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Oocyte aging in a marine protostome worm: The roles of maturation‐promoting factor and extracellular signal regulated kinase form of mitogen‐activated protein kinase</atitle><jtitle>Development, growth & differentiation</jtitle><addtitle>Dev Growth Differ</addtitle><date>2016-04</date><risdate>2016</risdate><volume>58</volume><issue>3</issue><spage>250</spage><epage>259</epage><pages>250-259</pages><issn>0012-1592</issn><eissn>1440-169X</eissn><coden>DGDFA5</coden><abstract>The roles of maturation‐promoting factor (MPF) and an extracellular signal regulated kinase form of mitogen‐activated protein kinase (ERK MAPK) are analyzed during oocyte aging in the marine protostome worm Cerebratulus. About a day after removal from the ovary, unfertilized metaphase‐I‐arrested oocytes of Cerebratulus begin to flatten and swell before eventually lysing, thereby exhibiting characteristics of a necroptotic mode of regulated cell death. Based on immunoblots probed with phospho‐specific antibodies, MPF and ERK are initially active in freshly mature specimens. However, as oocytes age, both kinase activities decline, with ERK deactivation occurring well before MPF downregulation. Experiments using pharmacological modulators indicate that oocyte degradation is promoted by the maturation‐initiated activation of ERK as well as by the deactivation of MPF that occurs in extensively aged specimens. The potential significance of these findings is discussed relative to previously published results for apoptotic eggs and oocytes of echinoderm and vertebrate deuterostomes.
To supplement studies of other oocytes, morphological patterns of extraovarian aging are described for metaphase‐I‐arrested oocytes produced by Cerebratulus, an externally fertilizing marine worm of the phylum Nemertea. In addition, pharmacological modulators are combined with immunoblotting assays to provide data for this protostome that can be compared with previous reports for echinoderm and vertebrate deuterostomes.</abstract><cop>Japan</cop><pub>Wiley Subscription Services, Inc</pub><pmid>26918273</pmid><doi>10.1111/dgd.12269</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Wiley Free Content; Freely Accessible Japanese Titles; Wiley Online Library All Journals |
| subjects | Animals apoptosis Apoptosis - physiology Butadienes - pharmacology Cells, Cultured Cellular Senescence - drug effects Cellular Senescence - physiology Cerebratulus Colforsin - pharmacology Extracellular Signal-Regulated MAP Kinases - antagonists & inhibitors Extracellular Signal-Regulated MAP Kinases - metabolism Female Immunoblotting Invertebrates - cytology Invertebrates - drug effects Invertebrates - metabolism Kinases Maturation-Promoting Factor - antagonists & inhibitors Maturation-Promoting Factor - metabolism necroptosis nemertean Nitriles - pharmacology non‐apoptotic regulated cell death Oocytes - cytology Oocytes - drug effects Oocytes - physiology Protein Kinase Inhibitors - pharmacology Purines - pharmacology Seawater - parasitology Time Factors Vasodilator Agents |
| title | Oocyte aging in a marine protostome worm: The roles of maturation‐promoting factor and extracellular signal regulated kinase form of mitogen‐activated protein kinase |
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