microplate assay to measure soil microbial biomass phosphorus
Quantification of phosphorus (P) concentrations in microbial biomass is required to better understand how P immobilization and turnover in soils are controlled by environmental and anthropogenic factors. Soil microbial biomass P (MBP) is generally extracted using the chloroform fumigation-direct ext...
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| Veröffentlicht in: | Biology and fertility of soils 2004-08, Vol.40 (3), p.201-205 |
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| creator | Jeannotte, R Sommerville, D.W Hamel, C Whalen, J.K |
| description | Quantification of phosphorus (P) concentrations in microbial biomass is required to better understand how P immobilization and turnover in soils are controlled by environmental and anthropogenic factors. Soil microbial biomass P (MBP) is generally extracted using the chloroform fumigation-direct extraction procedure and then analysed for P using the ammonium molybdate-ascorbic acid method on a flow injection analysis (FIA) system. Our objective was to determine whether a microscale malachite green method on a microplate system would provide as accurate MBP analysis as the ascorbic acid method on an FIA system. Twelve soils were collected from agricultural fields in southwestern Quebec, fumigated with chloroform and extracted with 0.5 M NaHCO3 (pH 8.5). The dissolved inorganic phosphorus (DIP) concentration in fumigated soils was not affected by the method of analysis, and results from the two systems of analysis were significantly correlated (r =0.998, P |
| doi_str_mv | 10.1007/s00374-004-0760-4 |
| format | Article |
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Soil microbial biomass P (MBP) is generally extracted using the chloroform fumigation-direct extraction procedure and then analysed for P using the ammonium molybdate-ascorbic acid method on a flow injection analysis (FIA) system. Our objective was to determine whether a microscale malachite green method on a microplate system would provide as accurate MBP analysis as the ascorbic acid method on an FIA system. Twelve soils were collected from agricultural fields in southwestern Quebec, fumigated with chloroform and extracted with 0.5 M NaHCO3 (pH 8.5). The dissolved inorganic phosphorus (DIP) concentration in fumigated soils was not affected by the method of analysis, and results from the two systems of analysis were significantly correlated (r =0.998, P<0.05). The MBP concentrations in these agricultural soils were between 0.36 and 60.05 micrograms P g-1, consistent with other published values. Our results indicate that MBP can be assessed equally well with the malachite green method using a microplate system as with the ascorbic acid method on an FIA system. The microplate system is rapid and requires smaller volumes of samples and reagents than the FIA system, thus reducing the quantity of waste produced. We conclude that the microscale malachite green method could be applied to measure the MBP concentration in a wide range of soils with good sensitivity, reproducibility and accuracy.</description><identifier>ISSN: 0178-2762</identifier><identifier>EISSN: 1432-0789</identifier><identifier>DOI: 10.1007/s00374-004-0760-4</identifier><identifier>CODEN: BFSOEE</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Agricultural land ; agricultural soils ; Agronomy. Soil science and plant productions ; Ammonium ; ammonium molybdate-ascorbic acid method ; ammonium molybdate-malaxhite green method ; analytical methods ; Anthropogenic factors ; ascorbic acid ; asparagus spears ; beans ; bioassays ; Biochemistry and biology ; Biological and medical sciences ; Biomass ; Carbon ; Chemical, physicochemical, biochemical and biological properties ; Chloroform ; dry matter accumulation ; Endoaquents ; flow injection analysis ; Fumigation ; Fundamental and applied biological sciences. Psychology ; Glycine max ; malachite green ; Microbiology ; microscale assays ; Phaseolus vulgaris ; Phosphorus ; Physics, chemistry, biochemistry and biology of agricultural and forest soils ; Reagents ; soil fumigation ; soil microorganisms ; Soil science ; soil texture ; Soils ; soybeans</subject><ispartof>Biology and fertility of soils, 2004-08, Vol.40 (3), p.201-205</ispartof><rights>2004 INIST-CNRS</rights><rights>Springer-Verlag 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c355t-a684d68974fa8db9131a205a50514588e206e60de719e2f6d450edd42b2d8d833</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16027575$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Jeannotte, R</creatorcontrib><creatorcontrib>Sommerville, D.W</creatorcontrib><creatorcontrib>Hamel, C</creatorcontrib><creatorcontrib>Whalen, J.K</creatorcontrib><title>microplate assay to measure soil microbial biomass phosphorus</title><title>Biology and fertility of soils</title><description>Quantification of phosphorus (P) concentrations in microbial biomass is required to better understand how P immobilization and turnover in soils are controlled by environmental and anthropogenic factors. Soil microbial biomass P (MBP) is generally extracted using the chloroform fumigation-direct extraction procedure and then analysed for P using the ammonium molybdate-ascorbic acid method on a flow injection analysis (FIA) system. Our objective was to determine whether a microscale malachite green method on a microplate system would provide as accurate MBP analysis as the ascorbic acid method on an FIA system. Twelve soils were collected from agricultural fields in southwestern Quebec, fumigated with chloroform and extracted with 0.5 M NaHCO3 (pH 8.5). The dissolved inorganic phosphorus (DIP) concentration in fumigated soils was not affected by the method of analysis, and results from the two systems of analysis were significantly correlated (r =0.998, P<0.05). The MBP concentrations in these agricultural soils were between 0.36 and 60.05 micrograms P g-1, consistent with other published values. Our results indicate that MBP can be assessed equally well with the malachite green method using a microplate system as with the ascorbic acid method on an FIA system. The microplate system is rapid and requires smaller volumes of samples and reagents than the FIA system, thus reducing the quantity of waste produced. We conclude that the microscale malachite green method could be applied to measure the MBP concentration in a wide range of soils with good sensitivity, reproducibility and accuracy.</description><subject>Agricultural land</subject><subject>agricultural soils</subject><subject>Agronomy. Soil science and plant productions</subject><subject>Ammonium</subject><subject>ammonium molybdate-ascorbic acid method</subject><subject>ammonium molybdate-malaxhite green method</subject><subject>analytical methods</subject><subject>Anthropogenic factors</subject><subject>ascorbic acid</subject><subject>asparagus spears</subject><subject>beans</subject><subject>bioassays</subject><subject>Biochemistry and biology</subject><subject>Biological and medical sciences</subject><subject>Biomass</subject><subject>Carbon</subject><subject>Chemical, physicochemical, biochemical and biological properties</subject><subject>Chloroform</subject><subject>dry matter accumulation</subject><subject>Endoaquents</subject><subject>flow injection analysis</subject><subject>Fumigation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycine max</subject><subject>malachite green</subject><subject>Microbiology</subject><subject>microscale assays</subject><subject>Phaseolus vulgaris</subject><subject>Phosphorus</subject><subject>Physics, chemistry, biochemistry and biology of agricultural and forest soils</subject><subject>Reagents</subject><subject>soil fumigation</subject><subject>soil microorganisms</subject><subject>Soil science</subject><subject>soil texture</subject><subject>Soils</subject><subject>soybeans</subject><issn>0178-2762</issn><issn>1432-0789</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpd0E1LxDAQBuAgCq6rP8CTRdBbdfKdHjyI-AWCB_UcZttUu7Sbmtke_PdGd0HwMAyBZ4bMy9gxhwsOYC8JQFpVAuSyBkq1w2ZcSZFfrtplM-DWlcIasc8OiJYAXDtezdjV0NUpjj2uQ4FE-FWsYzEEpCmFgmLXF79g0WFfLLo4ZFOMH5FypYkO2V6LPYWjbZ-zt7vb15uH8un5_vHm-qmspdbrEo1TjXGVVS26ZlFxyVGARg2aK-1cEGCCgSZYXgXRmkZpCE2jxEI0rnFSztn5Zu-Y4ucUaO2HjurQ97gKcSKfj4NKSJvh6T-4jFNa5b95w2UGwKuM-Ablw4hSaP2YugHTl-fgf9L0mzR9TtP_pOlVnjnbLkaqsW8TruqO_gYNCKutzu5k41qMHt9TNm8vArgEqLSUTshvwYl8mQ</recordid><startdate>20040801</startdate><enddate>20040801</enddate><creator>Jeannotte, R</creator><creator>Sommerville, D.W</creator><creator>Hamel, C</creator><creator>Whalen, J.K</creator><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SN</scope><scope>7T7</scope><scope>7UA</scope><scope>7X2</scope><scope>7XB</scope><scope>88I</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>H95</scope><scope>HCIFZ</scope><scope>L.G</scope><scope>LK8</scope><scope>M0K</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope></search><sort><creationdate>20040801</creationdate><title>microplate assay to measure soil microbial biomass phosphorus</title><author>Jeannotte, R ; Sommerville, D.W ; Hamel, C ; Whalen, J.K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c355t-a684d68974fa8db9131a205a50514588e206e60de719e2f6d450edd42b2d8d833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Agricultural land</topic><topic>agricultural soils</topic><topic>Agronomy. Soil science and plant productions</topic><topic>Ammonium</topic><topic>ammonium molybdate-ascorbic acid method</topic><topic>ammonium molybdate-malaxhite green method</topic><topic>analytical methods</topic><topic>Anthropogenic factors</topic><topic>ascorbic acid</topic><topic>asparagus spears</topic><topic>beans</topic><topic>bioassays</topic><topic>Biochemistry and biology</topic><topic>Biological and medical sciences</topic><topic>Biomass</topic><topic>Carbon</topic><topic>Chemical, physicochemical, biochemical and biological properties</topic><topic>Chloroform</topic><topic>dry matter accumulation</topic><topic>Endoaquents</topic><topic>flow injection analysis</topic><topic>Fumigation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycine max</topic><topic>malachite green</topic><topic>Microbiology</topic><topic>microscale assays</topic><topic>Phaseolus vulgaris</topic><topic>Phosphorus</topic><topic>Physics, chemistry, biochemistry and biology of agricultural and forest soils</topic><topic>Reagents</topic><topic>soil fumigation</topic><topic>soil microorganisms</topic><topic>Soil science</topic><topic>soil texture</topic><topic>Soils</topic><topic>soybeans</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jeannotte, R</creatorcontrib><creatorcontrib>Sommerville, D.W</creatorcontrib><creatorcontrib>Hamel, C</creatorcontrib><creatorcontrib>Whalen, J.K</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Ecology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Water Resources Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Science Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>SciTech Premium Collection</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>ProQuest Science Database</collection><collection>ProQuest Biological Science Journals</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><jtitle>Biology and fertility of soils</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jeannotte, R</au><au>Sommerville, D.W</au><au>Hamel, C</au><au>Whalen, J.K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>microplate assay to measure soil microbial biomass phosphorus</atitle><jtitle>Biology and fertility of soils</jtitle><date>2004-08-01</date><risdate>2004</risdate><volume>40</volume><issue>3</issue><spage>201</spage><epage>205</epage><pages>201-205</pages><issn>0178-2762</issn><eissn>1432-0789</eissn><coden>BFSOEE</coden><abstract>Quantification of phosphorus (P) concentrations in microbial biomass is required to better understand how P immobilization and turnover in soils are controlled by environmental and anthropogenic factors. Soil microbial biomass P (MBP) is generally extracted using the chloroform fumigation-direct extraction procedure and then analysed for P using the ammonium molybdate-ascorbic acid method on a flow injection analysis (FIA) system. Our objective was to determine whether a microscale malachite green method on a microplate system would provide as accurate MBP analysis as the ascorbic acid method on an FIA system. Twelve soils were collected from agricultural fields in southwestern Quebec, fumigated with chloroform and extracted with 0.5 M NaHCO3 (pH 8.5). The dissolved inorganic phosphorus (DIP) concentration in fumigated soils was not affected by the method of analysis, and results from the two systems of analysis were significantly correlated (r =0.998, P<0.05). The MBP concentrations in these agricultural soils were between 0.36 and 60.05 micrograms P g-1, consistent with other published values. Our results indicate that MBP can be assessed equally well with the malachite green method using a microplate system as with the ascorbic acid method on an FIA system. The microplate system is rapid and requires smaller volumes of samples and reagents than the FIA system, thus reducing the quantity of waste produced. We conclude that the microscale malachite green method could be applied to measure the MBP concentration in a wide range of soils with good sensitivity, reproducibility and accuracy.</abstract><cop>Berlin</cop><pub>Springer</pub><doi>10.1007/s00374-004-0760-4</doi><tpages>5</tpages></addata></record> |
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| subjects | Agricultural land agricultural soils Agronomy. Soil science and plant productions Ammonium ammonium molybdate-ascorbic acid method ammonium molybdate-malaxhite green method analytical methods Anthropogenic factors ascorbic acid asparagus spears beans bioassays Biochemistry and biology Biological and medical sciences Biomass Carbon Chemical, physicochemical, biochemical and biological properties Chloroform dry matter accumulation Endoaquents flow injection analysis Fumigation Fundamental and applied biological sciences. Psychology Glycine max malachite green Microbiology microscale assays Phaseolus vulgaris Phosphorus Physics, chemistry, biochemistry and biology of agricultural and forest soils Reagents soil fumigation soil microorganisms Soil science soil texture Soils soybeans |
| title | microplate assay to measure soil microbial biomass phosphorus |
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