Genetic variance of mating frequency in the honeybee (Apis mellifera L.)

The genetic variance of queen mating frequency was studied in honeybees (Apis mellifera carnica). Worker offspring (N = 966) of 28 naturally mated half sister-queens (r = 0.25) from seven unrelated breeding lines were genotyped at four DNA microsatellites. The mating frequencies of the queens were derived from the offspring genotypes. The number of observed matings per queen ranged from 10 to 28 with an average of 17.32 plus or minus 1.10 (number of estimated matings: 24.94 plus or minus 2.51; number of effective matings: 20.09 plus or minus 1.73). Half-sib analyses of the breeding lines were used to estimate heritability. Heritability was h super(2) = 0.449 plus or minus 0.135 for the estimated number of matings and h super(2) = 0.262 plus or minus 0.103 for the number of effective males, which are both significantly different from zero. We conclude that a high genetic variance for polyandry in honeybees can be favored by balanced selection between individual queen and colony level.