Automated N-glycan profiling of a mutant Trypanosoma rangeli sialidase expressed in Pichia pastoris, using tandem mass spectrometry and bioinformatics

A mutant Trypanosoma rangeli sialidase, Tr7, expressed in Pichia pastoris, exhibits significant trans-sialidase activity, and has been used for analytical-scale production of sialylated human milk oligosaccharides. Mass spectrometry-based site-specific N-glycoprofiling of Tr7 showed that heterogeneo...

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Veröffentlicht in:	Glycobiology (Oxford) 2015-12, Vol.25 (12), p.1350-1361
Hauptverfasser:	Li, Haiying, Rasmussen, Morten I, Larsen, Martin R, Guo, Yao, Jers, Carsten, Palmisano, Giuseppe, Mikkelsen, Jørn D, Kirpekar, Finn
Format:	Artikel
Sprache:	eng
Schlagworte:	Glycosylation Milk, Human - chemistry Mutation Neuraminidase - chemistry Neuraminidase - genetics Neuraminidase - metabolism Pichia - genetics Pichia - metabolism Pichia pastoris Polysaccharides - metabolism Protein Processing, Post-Translational Protozoan Proteins - chemistry Protozoan Proteins - genetics Protozoan Proteins - metabolism Recombinant Proteins Trypanosoma rangeli Trypanosoma rangeli - enzymology Trypanosoma rangeli - genetics
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