Isolation of Multipotent Mesenchymal Stromal Cells from Cryopreserved Human Umbilical Cord Tissue
Umbilical cord stroma is an easily available, convenient, and promising source of multipotent mesenchymal stromal cells for regenerative medicine. Cryogenic storage of umbilical cord tissue provides more possibilities for further isolation of multipotent mesenchymal stromal cells for autologous tran...
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| Veröffentlicht in: | Bulletin of experimental biology and medicine 2016-02, Vol.160 (4), p.530-534 |
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| container_title | Bulletin of experimental biology and medicine |
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| creator | Romanov, Yu. A. Balashova, E. E. Volgina, N. E. Kabaeva, N. V. Dugina, T. N. Sukhikh, G. T. |
| description | Umbilical cord stroma is an easily available, convenient, and promising source of multipotent mesenchymal stromal cells for regenerative medicine. Cryogenic storage of umbilical cord tissue provides more possibilities for further isolation of multipotent mesenchymal stromal cells for autologous transplantation or scientific purposes. Here we developed a protocol for preparation of the whole umbilical cord tissue for cryogenic storage that in combination with the previously described modified method of isolation of multipotent mesenchymal stromal cells allowed us to isolate cells with high proliferative potential, typical phenotype, and preserved differentiation potencies. |
| doi_str_mv | 10.1007/s10517-016-3213-9 |
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| subjects | Biological products Biomedical and Life Sciences Biomedicine Cell Biology Cell Culture Techniques Cell Differentiation Cell Proliferation Cells, Cultured Cryopreservation - methods Female Humans Internal Medicine Laboratory Medicine Mesenchymal Stem Cell Transplantation Mesenchymal Stromal Cells - cytology Pathology Stem cells Umbilical Cord - cytology |
| title | Isolation of Multipotent Mesenchymal Stromal Cells from Cryopreserved Human Umbilical Cord Tissue |
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