Inhibitory effects of Aster spathulifolius extract on adipogenesis and lipid accumulation in 3T3-L1 preadipocytes
Objectives Aster spathulifolius Maxim (AS), known for its anti‐viral and anti‐allergic activity, is also known to reduce body weight gain in high fat diet‐induced obese rats. But its molecular mechanism of the anti‐obesity effects is still unclear. So, we investigated the inhibitory effect of AS ext...
Gespeichert in:
| Veröffentlicht in: | Journal of pharmacy and pharmacology 2016-01, Vol.68 (1), p.107-118 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 118 |
|---|---|
| container_issue | 1 |
| container_start_page | 107 |
| container_title | Journal of pharmacy and pharmacology |
| container_volume | 68 |
| creator | Kim, Sa-Jic Choung, Se-Young |
| description | Objectives
Aster spathulifolius Maxim (AS), known for its anti‐viral and anti‐allergic activity, is also known to reduce body weight gain in high fat diet‐induced obese rats. But its molecular mechanism of the anti‐obesity effects is still unclear. So, we investigated the inhibitory effect of AS extract (ASE) on adipogenesis and lipid accumulation to determine the underlying cellular molecular mechanism.
Methods
To perform this study, the contents of intracellular triglyceride were analysed. Real‐time polymerase chain reaction and Western blotting were carried out to investigate the expression of adipogenic transcriptional factors.
Key findings
ASE showed the suppression of adipogenic differentiation and the considerable reduction of the lipid accumulation in 3T3‐L1 cells. Especially, ASE inhibited the early stage of differentiation via the downregulation of C/EBP‐β and C/EBP‐δ, which are early adipogenic factors. Major adipogenic factors, such as PPAR‐γ and C/EBP‐α, were also subsequently inhibited. These findings were supported by Oil Red O staining and intracellular triglyceride levels. A molecular mechanism liking the effect of ASE was identified through the activation of AMPKα pathway. ASE increased protein levels of phosphorylated AMPKα and phosphorylated ACC.
Conclusions
ASE showed anti‐adipogenic and anti‐lipogenic effects through the regulation of adipogenic factors and AMPKα pathway. |
| doi_str_mv | 10.1111/jphp.12485 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_1780522351</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3931489591</sourcerecordid><originalsourceid>FETCH-LOGICAL-i3525-ecefab23b31d1161d6d5eb9b12fcf33acaa211d2169f23c854e5e7b59f32fbd63</originalsourceid><addsrcrecordid>eNpdkUFv1DAQhS1ERbeFCz8AWeLSS1qPHdubY1VBW7SFHhYhcbEcZ8x6ySap7ajdf9_sbumBucxI873R6D1CPgI7h6ku1sNqOAdezuUbMuOs5IUGOX9LZoxxXgipxTE5SWnNGNNKqXfkmKtSQ6mqGXm47VahDrmPW4reo8uJ9p5epoyRpsHm1dgG37dhTBSfcrQu076jtglD_wc7TCFR2zW0DUNoqHVu3IytzWFiQkfFUhQLoEPEvcBtM6b35MjbNuGHl35Kfn79sry6KRY_rm-vLhdFEJLLAh16W3NRC2gAFDSqkVhXNXDvvBDWWcsBGg6q8ly4uSxRoq5l5QX3daPEKTk73B1i_zBiymYTksO2tR32YzKg50xyLiRM6Of_0HU_xm76bqJkJaDSekd9eqHGeoONGWLY2Lg1_8ycADgAj6HF7esemNnFZHYxmX1M5tv9zf1-mjTFQRMmx59eNTb-NUoLLc2v79dmSnOx_C3vzJ14BlVzlao</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1759319771</pqid></control><display><type>article</type><title>Inhibitory effects of Aster spathulifolius extract on adipogenesis and lipid accumulation in 3T3-L1 preadipocytes</title><source>MEDLINE</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Wiley Online Library All Journals</source><creator>Kim, Sa-Jic ; Choung, Se-Young</creator><creatorcontrib>Kim, Sa-Jic ; Choung, Se-Young</creatorcontrib><description>Objectives
Aster spathulifolius Maxim (AS), known for its anti‐viral and anti‐allergic activity, is also known to reduce body weight gain in high fat diet‐induced obese rats. But its molecular mechanism of the anti‐obesity effects is still unclear. So, we investigated the inhibitory effect of AS extract (ASE) on adipogenesis and lipid accumulation to determine the underlying cellular molecular mechanism.
Methods
To perform this study, the contents of intracellular triglyceride were analysed. Real‐time polymerase chain reaction and Western blotting were carried out to investigate the expression of adipogenic transcriptional factors.
Key findings
ASE showed the suppression of adipogenic differentiation and the considerable reduction of the lipid accumulation in 3T3‐L1 cells. Especially, ASE inhibited the early stage of differentiation via the downregulation of C/EBP‐β and C/EBP‐δ, which are early adipogenic factors. Major adipogenic factors, such as PPAR‐γ and C/EBP‐α, were also subsequently inhibited. These findings were supported by Oil Red O staining and intracellular triglyceride levels. A molecular mechanism liking the effect of ASE was identified through the activation of AMPKα pathway. ASE increased protein levels of phosphorylated AMPKα and phosphorylated ACC.
Conclusions
ASE showed anti‐adipogenic and anti‐lipogenic effects through the regulation of adipogenic factors and AMPKα pathway.</description><identifier>ISSN: 0022-3573</identifier><identifier>EISSN: 2042-7158</identifier><identifier>DOI: 10.1111/jphp.12485</identifier><identifier>PMID: 26471469</identifier><identifier>CODEN: JPPMAB</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>3T3-L1 Cells ; Adipocytes - drug effects ; Adipocytes - metabolism ; adipogenesis ; Adipogenesis - drug effects ; AMPKα ; Animals ; Aster Plant - chemistry ; Aster spathulifolius maxim ; CCAAT-Enhancer-Binding Protein-alpha - metabolism ; Cell Differentiation - drug effects ; Cell Line ; Down-Regulation - drug effects ; Lipid Metabolism - drug effects ; Lipids ; Mice ; Obesity - drug therapy ; Obesity - metabolism ; Phosphorylation - drug effects ; Plant Extracts - chemistry ; Plant Extracts - pharmacology ; PPAR gamma - metabolism ; PPAR-γ ; Rodents ; Signal Transduction - drug effects ; Studies ; Triglycerides - metabolism</subject><ispartof>Journal of pharmacy and pharmacology, 2016-01, Vol.68 (1), p.107-118</ispartof><rights>2015 Royal Pharmaceutical Society</rights><rights>2015 Royal Pharmaceutical Society.</rights><rights>Copyright © 2016 Royal Pharmaceutical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjphp.12485$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjphp.12485$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26471469$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Sa-Jic</creatorcontrib><creatorcontrib>Choung, Se-Young</creatorcontrib><title>Inhibitory effects of Aster spathulifolius extract on adipogenesis and lipid accumulation in 3T3-L1 preadipocytes</title><title>Journal of pharmacy and pharmacology</title><addtitle>J Pharm Pharmacol</addtitle><description>Objectives
Aster spathulifolius Maxim (AS), known for its anti‐viral and anti‐allergic activity, is also known to reduce body weight gain in high fat diet‐induced obese rats. But its molecular mechanism of the anti‐obesity effects is still unclear. So, we investigated the inhibitory effect of AS extract (ASE) on adipogenesis and lipid accumulation to determine the underlying cellular molecular mechanism.
Methods
To perform this study, the contents of intracellular triglyceride were analysed. Real‐time polymerase chain reaction and Western blotting were carried out to investigate the expression of adipogenic transcriptional factors.
Key findings
ASE showed the suppression of adipogenic differentiation and the considerable reduction of the lipid accumulation in 3T3‐L1 cells. Especially, ASE inhibited the early stage of differentiation via the downregulation of C/EBP‐β and C/EBP‐δ, which are early adipogenic factors. Major adipogenic factors, such as PPAR‐γ and C/EBP‐α, were also subsequently inhibited. These findings were supported by Oil Red O staining and intracellular triglyceride levels. A molecular mechanism liking the effect of ASE was identified through the activation of AMPKα pathway. ASE increased protein levels of phosphorylated AMPKα and phosphorylated ACC.
Conclusions
ASE showed anti‐adipogenic and anti‐lipogenic effects through the regulation of adipogenic factors and AMPKα pathway.</description><subject>3T3-L1 Cells</subject><subject>Adipocytes - drug effects</subject><subject>Adipocytes - metabolism</subject><subject>adipogenesis</subject><subject>Adipogenesis - drug effects</subject><subject>AMPKα</subject><subject>Animals</subject><subject>Aster Plant - chemistry</subject><subject>Aster spathulifolius maxim</subject><subject>CCAAT-Enhancer-Binding Protein-alpha - metabolism</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Line</subject><subject>Down-Regulation - drug effects</subject><subject>Lipid Metabolism - drug effects</subject><subject>Lipids</subject><subject>Mice</subject><subject>Obesity - drug therapy</subject><subject>Obesity - metabolism</subject><subject>Phosphorylation - drug effects</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - pharmacology</subject><subject>PPAR gamma - metabolism</subject><subject>PPAR-γ</subject><subject>Rodents</subject><subject>Signal Transduction - drug effects</subject><subject>Studies</subject><subject>Triglycerides - metabolism</subject><issn>0022-3573</issn><issn>2042-7158</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkUFv1DAQhS1ERbeFCz8AWeLSS1qPHdubY1VBW7SFHhYhcbEcZ8x6ySap7ajdf9_sbumBucxI873R6D1CPgI7h6ku1sNqOAdezuUbMuOs5IUGOX9LZoxxXgipxTE5SWnNGNNKqXfkmKtSQ6mqGXm47VahDrmPW4reo8uJ9p5epoyRpsHm1dgG37dhTBSfcrQu076jtglD_wc7TCFR2zW0DUNoqHVu3IytzWFiQkfFUhQLoEPEvcBtM6b35MjbNuGHl35Kfn79sry6KRY_rm-vLhdFEJLLAh16W3NRC2gAFDSqkVhXNXDvvBDWWcsBGg6q8ly4uSxRoq5l5QX3daPEKTk73B1i_zBiymYTksO2tR32YzKg50xyLiRM6Of_0HU_xm76bqJkJaDSekd9eqHGeoONGWLY2Lg1_8ycADgAj6HF7esemNnFZHYxmX1M5tv9zf1-mjTFQRMmx59eNTb-NUoLLc2v79dmSnOx_C3vzJ14BlVzlao</recordid><startdate>201601</startdate><enddate>201601</enddate><creator>Kim, Sa-Jic</creator><creator>Choung, Se-Young</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QP</scope><scope>7TK</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>201601</creationdate><title>Inhibitory effects of Aster spathulifolius extract on adipogenesis and lipid accumulation in 3T3-L1 preadipocytes</title><author>Kim, Sa-Jic ; Choung, Se-Young</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i3525-ecefab23b31d1161d6d5eb9b12fcf33acaa211d2169f23c854e5e7b59f32fbd63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>3T3-L1 Cells</topic><topic>Adipocytes - drug effects</topic><topic>Adipocytes - metabolism</topic><topic>adipogenesis</topic><topic>Adipogenesis - drug effects</topic><topic>AMPKα</topic><topic>Animals</topic><topic>Aster Plant - chemistry</topic><topic>Aster spathulifolius maxim</topic><topic>CCAAT-Enhancer-Binding Protein-alpha - metabolism</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Line</topic><topic>Down-Regulation - drug effects</topic><topic>Lipid Metabolism - drug effects</topic><topic>Lipids</topic><topic>Mice</topic><topic>Obesity - drug therapy</topic><topic>Obesity - metabolism</topic><topic>Phosphorylation - drug effects</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - pharmacology</topic><topic>PPAR gamma - metabolism</topic><topic>PPAR-γ</topic><topic>Rodents</topic><topic>Signal Transduction - drug effects</topic><topic>Studies</topic><topic>Triglycerides - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Sa-Jic</creatorcontrib><creatorcontrib>Choung, Se-Young</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Journal of pharmacy and pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Sa-Jic</au><au>Choung, Se-Young</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibitory effects of Aster spathulifolius extract on adipogenesis and lipid accumulation in 3T3-L1 preadipocytes</atitle><jtitle>Journal of pharmacy and pharmacology</jtitle><addtitle>J Pharm Pharmacol</addtitle><date>2016-01</date><risdate>2016</risdate><volume>68</volume><issue>1</issue><spage>107</spage><epage>118</epage><pages>107-118</pages><issn>0022-3573</issn><eissn>2042-7158</eissn><coden>JPPMAB</coden><abstract>Objectives
Aster spathulifolius Maxim (AS), known for its anti‐viral and anti‐allergic activity, is also known to reduce body weight gain in high fat diet‐induced obese rats. But its molecular mechanism of the anti‐obesity effects is still unclear. So, we investigated the inhibitory effect of AS extract (ASE) on adipogenesis and lipid accumulation to determine the underlying cellular molecular mechanism.
Methods
To perform this study, the contents of intracellular triglyceride were analysed. Real‐time polymerase chain reaction and Western blotting were carried out to investigate the expression of adipogenic transcriptional factors.
Key findings
ASE showed the suppression of adipogenic differentiation and the considerable reduction of the lipid accumulation in 3T3‐L1 cells. Especially, ASE inhibited the early stage of differentiation via the downregulation of C/EBP‐β and C/EBP‐δ, which are early adipogenic factors. Major adipogenic factors, such as PPAR‐γ and C/EBP‐α, were also subsequently inhibited. These findings were supported by Oil Red O staining and intracellular triglyceride levels. A molecular mechanism liking the effect of ASE was identified through the activation of AMPKα pathway. ASE increased protein levels of phosphorylated AMPKα and phosphorylated ACC.
Conclusions
ASE showed anti‐adipogenic and anti‐lipogenic effects through the regulation of adipogenic factors and AMPKα pathway.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>26471469</pmid><doi>10.1111/jphp.12485</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-3573 |
| ispartof | Journal of pharmacy and pharmacology, 2016-01, Vol.68 (1), p.107-118 |
| issn | 0022-3573 2042-7158 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1780522351 |
| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); Wiley Online Library All Journals |
| subjects | 3T3-L1 Cells Adipocytes - drug effects Adipocytes - metabolism adipogenesis Adipogenesis - drug effects AMPKα Animals Aster Plant - chemistry Aster spathulifolius maxim CCAAT-Enhancer-Binding Protein-alpha - metabolism Cell Differentiation - drug effects Cell Line Down-Regulation - drug effects Lipid Metabolism - drug effects Lipids Mice Obesity - drug therapy Obesity - metabolism Phosphorylation - drug effects Plant Extracts - chemistry Plant Extracts - pharmacology PPAR gamma - metabolism PPAR-γ Rodents Signal Transduction - drug effects Studies Triglycerides - metabolism |
| title | Inhibitory effects of Aster spathulifolius extract on adipogenesis and lipid accumulation in 3T3-L1 preadipocytes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-09T16%3A30%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Inhibitory%20effects%20of%20Aster%20spathulifolius%20extract%20on%20adipogenesis%20and%20lipid%20accumulation%20in%203T3-L1%20preadipocytes&rft.jtitle=Journal%20of%20pharmacy%20and%20pharmacology&rft.au=Kim,%20Sa-Jic&rft.date=2016-01&rft.volume=68&rft.issue=1&rft.spage=107&rft.epage=118&rft.pages=107-118&rft.issn=0022-3573&rft.eissn=2042-7158&rft.coden=JPPMAB&rft_id=info:doi/10.1111/jphp.12485&rft_dat=%3Cproquest_pubme%3E3931489591%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1759319771&rft_id=info:pmid/26471469&rfr_iscdi=true |