Influence of NO-Containing Gas Flow on Various Parameters of Energy Metabolism in Erythrocytes

Influence of NO-Containing Gas Flow on Various Parameters of Energy Metabolism in Erythrocytes

We studied the influence of NO-containing gas phase on some parameters of energy metabolism in human erythrocytes. Whole blood samples were aerated with gas flows from the Plazon instrument (NO concentrations 800 and 80 ppm) and from the experimental generator (75 ppm). Activity of lactate dehydroge...

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Veröffentlicht in:Bulletin of experimental biology and medicine 2014-11, Vol.158 (1), p.34-36
Hauptverfasser: Martusevich, A. K., Solov’yova, A. G., Peretyagin, S. P., Karelin, V. I., Selemir, V. D.
Format: Artikel
Sprache:eng
Schlagworte:
Biomedical and Life Sciences
Biomedicine
Cell Biology
Energy Metabolism - drug effects
Erythrocytes - drug effects
Erythrocytes - metabolism
Gases
Humans
Internal Medicine
L-Lactate Dehydrogenase - metabolism
Laboratory Medicine
Nitric Oxide - pharmacology
Pathology
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Zusammenfassung:We studied the influence of NO-containing gas phase on some parameters of energy metabolism in human erythrocytes. Whole blood samples were aerated with gas flows from the Plazon instrument (NO concentrations 800 and 80 ppm) and from the experimental generator (75 ppm). Activity of lactate dehydrogenase in direct and reverse reactions, lactate level, and a number of derived coefficients were estimated. Treatment of blood with 800 ppm NO inhibited erythrocyte energy metabolism, and its 10-fold dilution attenuated the effect. The use of ROS-free gas flow containing 75 ppm of NO promoted optimization of the process under investigation.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-014-2685-8