Skin mucus proteome map of European sea bass (Dicentrarchus labrax)
Skin mucus is the first barrier of fish defence. Proteins from skin mucus of European sea bass (Dicentrarchus labrax) were identified by 2DE followed by LC‐MS/MS. From all the identified proteins in the proteome map, we focus on the proteins associated with several immune pathways in fish. Furthermo...
Gespeichert in:
| Veröffentlicht in: | Proteomics (Weinheim) 2015-12, Vol.15 (23-24), p.4007-4020 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 4020 |
|---|---|
| container_issue | 23-24 |
| container_start_page | 4007 |
| container_title | Proteomics (Weinheim) |
| container_volume | 15 |
| creator | Cordero, Héctor Brinchmann, Monica F. Cuesta, Alberto Meseguer, José Esteban, María A. |
| description | Skin mucus is the first barrier of fish defence. Proteins from skin mucus of European sea bass (Dicentrarchus labrax) were identified by 2DE followed by LC‐MS/MS. From all the identified proteins in the proteome map, we focus on the proteins associated with several immune pathways in fish. Furthermore, the real‐time PCR transcript levels in skin are shown. Proteins found include apolipoprotein A1, calmodulin, complement C3, fucose‐binding lectin, lysozyme and several caspases. To our knowledge, this is the first skin mucus proteome study and further transcriptional profiling of the identified proteins done on this bony fish species. This not only contributes knowledge on the routes involved in mucosal innate immunity, but also establishes a non‐invasive technique based on locating immune markers with a potential use for prevention and/or diagnosis of fish diseases. |
| doi_str_mv | 10.1002/pmic.201500120 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1780515612</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1760874188</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5509-7c19fb97a0ce656068846b63153358783ecf826dc4ad49c1cfb6f66b3f5202393</originalsourceid><addsrcrecordid>eNqNkb1PHDEQxa0IFD6SNmW0UhpS7DFjr7_KcOEA6YBIEF1peX1esbBfsW8V-O_x6eAKGqhmit97mnmPkG8IEwSgx0NbuwkF5ABI4RPZR4E810rgznbnbI8cxHifEKm0_Ez2qGBSUJD7ZHrzUHdZO7oxZkPoV75vfdbaIeur7HQM_eBtl0Vvs9LGmB39rp3vVsEGd5cEjS2Dffz5hexWton-68s8JH9np7fT83x-fXYx_TXPHeegc-lQV6WWFpwXXIBQqhClYMgZ40oq5l2lqFi6wi4L7dBVpaiEKFnFKVCm2SE52vimQ_-NPq5MW0fnm8Z2vh-jSd8BRy6QfgAVoGSBSiX0xxv0vh9Dlx5JFJdcA2hM1GRDudDHGHxlhlC3NjwZBLNuwqybMNsmkuD7i-1Ytn65xV-jTwDfAP_rxj-9Y2f-XF5MkWKxTiHf6Oq48o9bnQ0PRkgmuVlcnZnFfFaczBYnRrJnolOgWQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1757590091</pqid></control><display><type>article</type><title>Skin mucus proteome map of European sea bass (Dicentrarchus labrax)</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Cordero, Héctor ; Brinchmann, Monica F. ; Cuesta, Alberto ; Meseguer, José ; Esteban, María A.</creator><creatorcontrib>Cordero, Héctor ; Brinchmann, Monica F. ; Cuesta, Alberto ; Meseguer, José ; Esteban, María A.</creatorcontrib><description>Skin mucus is the first barrier of fish defence. Proteins from skin mucus of European sea bass (Dicentrarchus labrax) were identified by 2DE followed by LC‐MS/MS. From all the identified proteins in the proteome map, we focus on the proteins associated with several immune pathways in fish. Furthermore, the real‐time PCR transcript levels in skin are shown. Proteins found include apolipoprotein A1, calmodulin, complement C3, fucose‐binding lectin, lysozyme and several caspases. To our knowledge, this is the first skin mucus proteome study and further transcriptional profiling of the identified proteins done on this bony fish species. This not only contributes knowledge on the routes involved in mucosal innate immunity, but also establishes a non‐invasive technique based on locating immune markers with a potential use for prevention and/or diagnosis of fish diseases.</description><identifier>ISSN: 1615-9853</identifier><identifier>EISSN: 1615-9861</identifier><identifier>DOI: 10.1002/pmic.201500120</identifier><identifier>PMID: 26376207</identifier><language>eng</language><publisher>Germany: Blackwell Publishing Ltd</publisher><subject>Animals ; Bass - metabolism ; Dicentrarchus labrax ; European sea bass (Dicentrarchus labrax) ; Fish barriers ; Fish diseases ; Fish hatcheries ; Mucosal immunity ; Mucus - metabolism ; Proteins ; Proteome ; Proteome - metabolism ; Real-Time Polymerase Chain Reaction ; Skin - metabolism ; Skin mucus ; Tandem Mass Spectrometry ; Transcript profile</subject><ispartof>Proteomics (Weinheim), 2015-12, Vol.15 (23-24), p.4007-4020</ispartof><rights>2015 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><rights>2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5509-7c19fb97a0ce656068846b63153358783ecf826dc4ad49c1cfb6f66b3f5202393</citedby><cites>FETCH-LOGICAL-c5509-7c19fb97a0ce656068846b63153358783ecf826dc4ad49c1cfb6f66b3f5202393</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fpmic.201500120$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fpmic.201500120$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26376207$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cordero, Héctor</creatorcontrib><creatorcontrib>Brinchmann, Monica F.</creatorcontrib><creatorcontrib>Cuesta, Alberto</creatorcontrib><creatorcontrib>Meseguer, José</creatorcontrib><creatorcontrib>Esteban, María A.</creatorcontrib><title>Skin mucus proteome map of European sea bass (Dicentrarchus labrax)</title><title>Proteomics (Weinheim)</title><addtitle>Proteomics</addtitle><description>Skin mucus is the first barrier of fish defence. Proteins from skin mucus of European sea bass (Dicentrarchus labrax) were identified by 2DE followed by LC‐MS/MS. From all the identified proteins in the proteome map, we focus on the proteins associated with several immune pathways in fish. Furthermore, the real‐time PCR transcript levels in skin are shown. Proteins found include apolipoprotein A1, calmodulin, complement C3, fucose‐binding lectin, lysozyme and several caspases. To our knowledge, this is the first skin mucus proteome study and further transcriptional profiling of the identified proteins done on this bony fish species. This not only contributes knowledge on the routes involved in mucosal innate immunity, but also establishes a non‐invasive technique based on locating immune markers with a potential use for prevention and/or diagnosis of fish diseases.</description><subject>Animals</subject><subject>Bass - metabolism</subject><subject>Dicentrarchus labrax</subject><subject>European sea bass (Dicentrarchus labrax)</subject><subject>Fish barriers</subject><subject>Fish diseases</subject><subject>Fish hatcheries</subject><subject>Mucosal immunity</subject><subject>Mucus - metabolism</subject><subject>Proteins</subject><subject>Proteome</subject><subject>Proteome - metabolism</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Skin - metabolism</subject><subject>Skin mucus</subject><subject>Tandem Mass Spectrometry</subject><subject>Transcript profile</subject><issn>1615-9853</issn><issn>1615-9861</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkb1PHDEQxa0IFD6SNmW0UhpS7DFjr7_KcOEA6YBIEF1peX1esbBfsW8V-O_x6eAKGqhmit97mnmPkG8IEwSgx0NbuwkF5ABI4RPZR4E810rgznbnbI8cxHifEKm0_Ez2qGBSUJD7ZHrzUHdZO7oxZkPoV75vfdbaIeur7HQM_eBtl0Vvs9LGmB39rp3vVsEGd5cEjS2Dffz5hexWton-68s8JH9np7fT83x-fXYx_TXPHeegc-lQV6WWFpwXXIBQqhClYMgZ40oq5l2lqFi6wi4L7dBVpaiEKFnFKVCm2SE52vimQ_-NPq5MW0fnm8Z2vh-jSd8BRy6QfgAVoGSBSiX0xxv0vh9Dlx5JFJdcA2hM1GRDudDHGHxlhlC3NjwZBLNuwqybMNsmkuD7i-1Ytn65xV-jTwDfAP_rxj-9Y2f-XF5MkWKxTiHf6Oq48o9bnQ0PRkgmuVlcnZnFfFaczBYnRrJnolOgWQ</recordid><startdate>201512</startdate><enddate>201512</enddate><creator>Cordero, Héctor</creator><creator>Brinchmann, Monica F.</creator><creator>Cuesta, Alberto</creator><creator>Meseguer, José</creator><creator>Esteban, María A.</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201512</creationdate><title>Skin mucus proteome map of European sea bass (Dicentrarchus labrax)</title><author>Cordero, Héctor ; Brinchmann, Monica F. ; Cuesta, Alberto ; Meseguer, José ; Esteban, María A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5509-7c19fb97a0ce656068846b63153358783ecf826dc4ad49c1cfb6f66b3f5202393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Bass - metabolism</topic><topic>Dicentrarchus labrax</topic><topic>European sea bass (Dicentrarchus labrax)</topic><topic>Fish barriers</topic><topic>Fish diseases</topic><topic>Fish hatcheries</topic><topic>Mucosal immunity</topic><topic>Mucus - metabolism</topic><topic>Proteins</topic><topic>Proteome</topic><topic>Proteome - metabolism</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Skin - metabolism</topic><topic>Skin mucus</topic><topic>Tandem Mass Spectrometry</topic><topic>Transcript profile</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cordero, Héctor</creatorcontrib><creatorcontrib>Brinchmann, Monica F.</creatorcontrib><creatorcontrib>Cuesta, Alberto</creatorcontrib><creatorcontrib>Meseguer, José</creatorcontrib><creatorcontrib>Esteban, María A.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Proteomics (Weinheim)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cordero, Héctor</au><au>Brinchmann, Monica F.</au><au>Cuesta, Alberto</au><au>Meseguer, José</au><au>Esteban, María A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Skin mucus proteome map of European sea bass (Dicentrarchus labrax)</atitle><jtitle>Proteomics (Weinheim)</jtitle><addtitle>Proteomics</addtitle><date>2015-12</date><risdate>2015</risdate><volume>15</volume><issue>23-24</issue><spage>4007</spage><epage>4020</epage><pages>4007-4020</pages><issn>1615-9853</issn><eissn>1615-9861</eissn><abstract>Skin mucus is the first barrier of fish defence. Proteins from skin mucus of European sea bass (Dicentrarchus labrax) were identified by 2DE followed by LC‐MS/MS. From all the identified proteins in the proteome map, we focus on the proteins associated with several immune pathways in fish. Furthermore, the real‐time PCR transcript levels in skin are shown. Proteins found include apolipoprotein A1, calmodulin, complement C3, fucose‐binding lectin, lysozyme and several caspases. To our knowledge, this is the first skin mucus proteome study and further transcriptional profiling of the identified proteins done on this bony fish species. This not only contributes knowledge on the routes involved in mucosal innate immunity, but also establishes a non‐invasive technique based on locating immune markers with a potential use for prevention and/or diagnosis of fish diseases.</abstract><cop>Germany</cop><pub>Blackwell Publishing Ltd</pub><pmid>26376207</pmid><doi>10.1002/pmic.201500120</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1615-9853 |
| ispartof | Proteomics (Weinheim), 2015-12, Vol.15 (23-24), p.4007-4020 |
| issn | 1615-9853 1615-9861 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1780515612 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Bass - metabolism Dicentrarchus labrax European sea bass (Dicentrarchus labrax) Fish barriers Fish diseases Fish hatcheries Mucosal immunity Mucus - metabolism Proteins Proteome Proteome - metabolism Real-Time Polymerase Chain Reaction Skin - metabolism Skin mucus Tandem Mass Spectrometry Transcript profile |
| title | Skin mucus proteome map of European sea bass (Dicentrarchus labrax) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-25T02%3A19%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Skin%20mucus%20proteome%20map%20of%20European%20sea%20bass%20(Dicentrarchus%20labrax)&rft.jtitle=Proteomics%20(Weinheim)&rft.au=Cordero,%20H%C3%A9ctor&rft.date=2015-12&rft.volume=15&rft.issue=23-24&rft.spage=4007&rft.epage=4020&rft.pages=4007-4020&rft.issn=1615-9853&rft.eissn=1615-9861&rft_id=info:doi/10.1002/pmic.201500120&rft_dat=%3Cproquest_cross%3E1760874188%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1757590091&rft_id=info:pmid/26376207&rfr_iscdi=true |