Antitumor Activity of Rat Mesenchymal Stem Cells during Direct or Indirect Co-Culturing with C6 Glioma Cells
The tumor-suppressive effect of rat mesenchymal stem cells against low-differentiated rat C6 glioma cells during their direct and indirect co-culturing and during culturing of C6 glioma cells in the medium conditioned by mesenchymal stem cells was studied in an in vitro experiment. The most pronounc...
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| Veröffentlicht in: | Bulletin of experimental biology and medicine 2016-02, Vol.160 (4), p.519-524 |
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| creator | Gabashvili, A. N. Baklaushev, V. P. Grinenko, N. F. Mel’nikov, P. A. Cherepanov, S. A. Levinsky, A. B. Chehonin, V. P. |
| description | The tumor-suppressive effect of rat mesenchymal stem cells against low-differentiated rat C6 glioma cells during their direct and indirect co-culturing and during culturing of C6 glioma cells in the medium conditioned by mesenchymal stem cells was studied in an
in vitro
experiment. The most pronounced antitumor activity of mesenchymal stem cells was observed during direct co-culturing with C6 glioma cells. The number of live C6 glioma cells during indirect co-culturing and during culturing in conditioned medium was slightly higher than during direct co-culturing, but significantly differed from the control (C6 glioma cells cultured in medium conditioned by C6 glioma cells). The cytotoxic effect of medium conditioned by mesenchymal stem cells was not related to medium depletion by glioma cells during their growth. The medium conditioned by other “non-stem” cells (rat astrocytes and fibroblasts) produced no tumor-suppressive effect. Rat mesenchymal stem cells, similar to rat C6 glioma cells express connexin 43, the main astroglial gap junction protein. During co-culturing, mesenchymal stem cells and glioma C6 cells formed functionally active gap junctions. Gap junction blockade with connexon inhibitor carbenoxolone attenuated the antitumor effect observed during direct co-culturing of C6 glioma cells and mesenchymal stem cells to the level produced by conditioned medium. Cell–cell signaling mediated by gap junctions can be a mechanism of the tumor-suppressive effect of mesenchymal stem cells against C6 glioma cells. This phenomenon can be used for the development of new methods of cell therapy for high-grade malignant gliomas. |
| doi_str_mv | 10.1007/s10517-016-3211-y |
| format | Article |
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in vitro
experiment. The most pronounced antitumor activity of mesenchymal stem cells was observed during direct co-culturing with C6 glioma cells. The number of live C6 glioma cells during indirect co-culturing and during culturing in conditioned medium was slightly higher than during direct co-culturing, but significantly differed from the control (C6 glioma cells cultured in medium conditioned by C6 glioma cells). The cytotoxic effect of medium conditioned by mesenchymal stem cells was not related to medium depletion by glioma cells during their growth. The medium conditioned by other “non-stem” cells (rat astrocytes and fibroblasts) produced no tumor-suppressive effect. Rat mesenchymal stem cells, similar to rat C6 glioma cells express connexin 43, the main astroglial gap junction protein. During co-culturing, mesenchymal stem cells and glioma C6 cells formed functionally active gap junctions. Gap junction blockade with connexon inhibitor carbenoxolone attenuated the antitumor effect observed during direct co-culturing of C6 glioma cells and mesenchymal stem cells to the level produced by conditioned medium. Cell–cell signaling mediated by gap junctions can be a mechanism of the tumor-suppressive effect of mesenchymal stem cells against C6 glioma cells. This phenomenon can be used for the development of new methods of cell therapy for high-grade malignant gliomas.</description><subject>Animals</subject><subject>Antineoplastic Agents</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Brain Neoplasms - pathology</subject><subject>Cell Biology</subject><subject>Cell Communication - physiology</subject><subject>Cell Differentiation</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement</subject><subject>Cell Technologies in Biology and Medicine</subject><subject>Cell- and Tissue-Based Therapy</subject><subject>Coculture Techniques</subject><subject>Culture Media, Conditioned - pharmacology</subject><subject>Glioma - pathology</subject><subject>Gliomas</subject><subject>Internal Medicine</subject><subject>Laboratory Medicine</subject><subject>Mesenchymal Stromal Cells - metabolism</subject><subject>Pathology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Signal Transduction - physiology</subject><subject>Stem cells</subject><subject>Tight Junctions - physiology</subject><issn>0007-4888</issn><issn>1573-8221</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkl-P1CAUxYnRuOPqB_DFkJgYX7pygQJ9nFRdN1lj4p9nwlC6w6YtI1DNfHtpuuqu0cTwAFx-54QLB6GnQM6AEPkqAalBVgRExShAdbyHNlBLVilK4T7akAJVXCl1gh6ldL1siYCH6ISKhlAm6AYN2yn7PI8h4q3N_pvPRxx6_NFk_N4lN9n9cTQD_pTdiFs3DAl3c_TTFX7to7MZF93F1K3rNlTtPOT1_LvPe9wKfD74MJpV-xg96M2Q3JOb-RR9efvmc_uuuvxwftFuLytbE5arjgpDXdf0kkFjGTWKSi5UzToAaYET6BvbcyF2u15JQpwRSnFeCjXZKWXYKXq5-h5i-Dq7lPXoky03MJMLc9IgVXk4Jrj8D1QSpUjDVEGf_4FehzlOpZGFAiANSP6bujKD037qQ47GLqZ6y2vKG14LUaizv1BldG70Nkyu96V-R_DilmDvzJD3KQxz9mFKd0FYQRtDStH1-hD9aOJRA9FLavSaGl1So5fU6GPRPLvpbN6Nrvul-BmTAtAVSIflc1281fo_XX8AenjITA</recordid><startdate>20160201</startdate><enddate>20160201</enddate><creator>Gabashvili, A. 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N.</au><au>Baklaushev, V. P.</au><au>Grinenko, N. F.</au><au>Mel’nikov, P. A.</au><au>Cherepanov, S. A.</au><au>Levinsky, A. B.</au><au>Chehonin, V. P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antitumor Activity of Rat Mesenchymal Stem Cells during Direct or Indirect Co-Culturing with C6 Glioma Cells</atitle><jtitle>Bulletin of experimental biology and medicine</jtitle><stitle>Bull Exp Biol Med</stitle><addtitle>Bull Exp Biol Med</addtitle><date>2016-02-01</date><risdate>2016</risdate><volume>160</volume><issue>4</issue><spage>519</spage><epage>524</epage><pages>519-524</pages><issn>0007-4888</issn><eissn>1573-8221</eissn><coden>BEXBAN</coden><abstract>The tumor-suppressive effect of rat mesenchymal stem cells against low-differentiated rat C6 glioma cells during their direct and indirect co-culturing and during culturing of C6 glioma cells in the medium conditioned by mesenchymal stem cells was studied in an
in vitro
experiment. The most pronounced antitumor activity of mesenchymal stem cells was observed during direct co-culturing with C6 glioma cells. The number of live C6 glioma cells during indirect co-culturing and during culturing in conditioned medium was slightly higher than during direct co-culturing, but significantly differed from the control (C6 glioma cells cultured in medium conditioned by C6 glioma cells). The cytotoxic effect of medium conditioned by mesenchymal stem cells was not related to medium depletion by glioma cells during their growth. The medium conditioned by other “non-stem” cells (rat astrocytes and fibroblasts) produced no tumor-suppressive effect. Rat mesenchymal stem cells, similar to rat C6 glioma cells express connexin 43, the main astroglial gap junction protein. During co-culturing, mesenchymal stem cells and glioma C6 cells formed functionally active gap junctions. Gap junction blockade with connexon inhibitor carbenoxolone attenuated the antitumor effect observed during direct co-culturing of C6 glioma cells and mesenchymal stem cells to the level produced by conditioned medium. Cell–cell signaling mediated by gap junctions can be a mechanism of the tumor-suppressive effect of mesenchymal stem cells against C6 glioma cells. This phenomenon can be used for the development of new methods of cell therapy for high-grade malignant gliomas.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>26902362</pmid><doi>10.1007/s10517-016-3211-y</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Antineoplastic Agents Biomedical and Life Sciences Biomedicine Brain Neoplasms - pathology Cell Biology Cell Communication - physiology Cell Differentiation Cell Line, Tumor Cell Movement Cell Technologies in Biology and Medicine Cell- and Tissue-Based Therapy Coculture Techniques Culture Media, Conditioned - pharmacology Glioma - pathology Gliomas Internal Medicine Laboratory Medicine Mesenchymal Stromal Cells - metabolism Pathology Rats Rats, Wistar Signal Transduction - physiology Stem cells Tight Junctions - physiology |
| title | Antitumor Activity of Rat Mesenchymal Stem Cells during Direct or Indirect Co-Culturing with C6 Glioma Cells |
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