Subcellular calcium dynamics during juvenile development in mouse hippocampal astrocytes

Astrocytes generate calcium signals throughout their fine processes, which are assumed to locally regulate neighbouring neurotransmission and blood flow. The intercellular morphological relationships mature during juvenile periods when astrocytes elongate highly ramified processes. In this study, we examined developmental changes in calcium activity patterns of single hippocampal astrocytes using a transgenic mouse line in which astrocytes selectively express a genetically encoded calcium indicator, Yellow Cameleon‐Nano50. Compared with postnatal day 7, astrocytes at postnatal day 30 showed larger subcellular calcium events and a greater proportion of somatic events. At both ages, the calcium activity was abolished by removal of extracellular calcium ions. Calcium events in late juvenile astrocytes were not affected by spontaneously occurring sharp waves that trigger synchronized neuronal spikes, implying the independence of astrocyte calcium signals from neuronal synchronization. These results demonstrate that astrocytes undergo dynamic changes in their activity patterns during juvenile development. This study examined developmental changes in calcium activity of hippocampal astrocytes using transgenic mice in which astrocytes express a calcium indicator, Yellow Cameleon‐Nano50 (YC‐Nano50). Compared with postnatal day 7 (P7), astrocytes at P30 showed larger subcellular calcium events and a greater proportion of somatic events. The calcium events were independent from neuronal activity. The results demonstrate that astrocyte activity dynamically changes during juvenile development.