Live and heat-treated probiotics differently modulate IL10 mRNA stabilization and microRNA expression
In inflammatory diseases and healthy animals, studies have shown a probiotic-induced IL-10 overproduction1 or an increase in regulatory FoxP3+ cell number.2 Interestingly, in vivo and in vitro studies have convincingly demonstrated differential activities of live and heat-treated organisms.3 Yet, li...
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| Veröffentlicht in: | Journal of allergy and clinical immunology 2016-04, Vol.137 (4), p.1264-1267.e10 |
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| creator | Demont, Audrey, BS Hacini-Rachinel, Feriel, PhD Doucet-Ladevèze, Rémi, MS Ngom-Bru, Catherine, PhD Mercenier, Annick, PhD Prioult, Guénolée, PhD Blanchard, Carine, PhD |
| description | In inflammatory diseases and healthy animals, studies have shown a probiotic-induced IL-10 overproduction1 or an increase in regulatory FoxP3+ cell number.2 Interestingly, in vivo and in vitro studies have convincingly demonstrated differential activities of live and heat-treated organisms.3 Yet, little is known about the molecular mechanism involved in this modulation of IL10 mRNA expression. There are several AU-rich elements in the 3' untranslated region (UTR) of IL10 mRNA that have been shown to regulate IL10 gene expression level.4 PBMCs, transfected with a plasmid containing the IL10-3'UTR downstream of the luciferase (Luc) gene (Luc/IL10-3'UTR) and stimulated by heat-treated L paracasei NCC 2461 produced a significantly higher level of luciferase activity (3.6 ± 0.6 vs 0.6 ± 0.11; P < .05) than did transfected unstimulated cells (Fig 2, A), suggesting a role for IL10-3'UTR in heat-treated L paracasei NCC 2461-induced IL10 mRNA stabilization. |
| doi_str_mv | 10.1016/j.jaci.2015.08.033 |
| format | Article |
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There are several AU-rich elements in the 3' untranslated region (UTR) of IL10 mRNA that have been shown to regulate IL10 gene expression level.4 PBMCs, transfected with a plasmid containing the IL10-3'UTR downstream of the luciferase (Luc) gene (Luc/IL10-3'UTR) and stimulated by heat-treated L paracasei NCC 2461 produced a significantly higher level of luciferase activity (3.6 ± 0.6 vs 0.6 ± 0.11; P < .05) than did transfected unstimulated cells (Fig 2, A), suggesting a role for IL10-3'UTR in heat-treated L paracasei NCC 2461-induced IL10 mRNA stabilization.</description><identifier>ISSN: 0091-6749</identifier><identifier>EISSN: 1097-6825</identifier><identifier>DOI: 10.1016/j.jaci.2015.08.033</identifier><identifier>PMID: 26478009</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Allergy and Immunology ; Biomarkers - metabolism ; Cytokines ; Gene expression ; Gene Expression Regulation ; Hot Temperature ; Humans ; Interleukin-10 - genetics ; Interleukin-10 - immunology ; Lactobacillus - immunology ; Lactobacillus - physiology ; Leukocytes, Mononuclear - immunology ; Leukocytes, Mononuclear - microbiology ; Microorganisms ; MicroRNAs ; MicroRNAs - immunology ; Physiology ; Probiotics ; Probiotics - pharmacology</subject><ispartof>Journal of allergy and clinical immunology, 2016-04, Vol.137 (4), p.1264-1267.e10</ispartof><rights>American Academy of Allergy, Asthma & Immunology</rights><rights>2015 American Academy of Allergy, Asthma & Immunology</rights><rights>Copyright Elsevier Limited Apr 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c483t-4d84b7318b0daa28f634d3b7ef7e65a095910220f0af4c00b56f10b56a7d23b3</citedby><cites>FETCH-LOGICAL-c483t-4d84b7318b0daa28f634d3b7ef7e65a095910220f0af4c00b56f10b56a7d23b3</cites><orcidid>0000-0002-3377-290X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0091674915012579$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26478009$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Demont, Audrey, BS</creatorcontrib><creatorcontrib>Hacini-Rachinel, Feriel, PhD</creatorcontrib><creatorcontrib>Doucet-Ladevèze, Rémi, MS</creatorcontrib><creatorcontrib>Ngom-Bru, Catherine, PhD</creatorcontrib><creatorcontrib>Mercenier, Annick, PhD</creatorcontrib><creatorcontrib>Prioult, Guénolée, PhD</creatorcontrib><creatorcontrib>Blanchard, Carine, PhD</creatorcontrib><title>Live and heat-treated probiotics differently modulate IL10 mRNA stabilization and microRNA expression</title><title>Journal of allergy and clinical immunology</title><addtitle>J Allergy Clin Immunol</addtitle><description>In inflammatory diseases and healthy animals, studies have shown a probiotic-induced IL-10 overproduction1 or an increase in regulatory FoxP3+ cell number.2 Interestingly, in vivo and in vitro studies have convincingly demonstrated differential activities of live and heat-treated organisms.3 Yet, little is known about the molecular mechanism involved in this modulation of IL10 mRNA expression. There are several AU-rich elements in the 3' untranslated region (UTR) of IL10 mRNA that have been shown to regulate IL10 gene expression level.4 PBMCs, transfected with a plasmid containing the IL10-3'UTR downstream of the luciferase (Luc) gene (Luc/IL10-3'UTR) and stimulated by heat-treated L paracasei NCC 2461 produced a significantly higher level of luciferase activity (3.6 ± 0.6 vs 0.6 ± 0.11; P < .05) than did transfected unstimulated cells (Fig 2, A), suggesting a role for IL10-3'UTR in heat-treated L paracasei NCC 2461-induced IL10 mRNA stabilization.</description><subject>Allergy and Immunology</subject><subject>Biomarkers - metabolism</subject><subject>Cytokines</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Hot Temperature</subject><subject>Humans</subject><subject>Interleukin-10 - genetics</subject><subject>Interleukin-10 - immunology</subject><subject>Lactobacillus - immunology</subject><subject>Lactobacillus - physiology</subject><subject>Leukocytes, Mononuclear - immunology</subject><subject>Leukocytes, Mononuclear - microbiology</subject><subject>Microorganisms</subject><subject>MicroRNAs</subject><subject>MicroRNAs - immunology</subject><subject>Physiology</subject><subject>Probiotics</subject><subject>Probiotics - pharmacology</subject><issn>0091-6749</issn><issn>1097-6825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kl2L1TAQhoMo7nH1D3ghBW-8aZ0kTZOCCMvix8JBQfc-pMkUU_txTNJlj7_e1LMq7IU3E5I88zIz7xDynEJFgTavh2ow1lcMqKhAVcD5A7Kj0MqyUUw8JDuAlpaNrNsz8iTGAfKdq_YxOWNNLVW-7Qju_Q0WZnbFNzSpTCFHdMUhLJ1fkrexcL7vMeCcxmMxLW4dM1Bc7SkU05dPF0VMpvOj_2mSX-bfQpO3Ydm-8PYQMMb8_pQ86s0Y8dndeU6u37-7vvxY7j9_uLq82Je2VjyVtVN1JzlVHThjmOobXjveSewlNsJAK1oKjEEPpq8tQCeanm7RSMd4x8_Jq5NsLv_HijHpyUeL42hmXNaoqZStUlQwyOjLe-iwrGHOxWVKgYRaCJUpdqJyRzEG7PUh-MmEo6agNw_0oDcP9OaBBqWzBznpxZ302k3o_qb8GXoG3pwAzKO48Rh0tB5ni84HtEm7xf9f_-29dDv62Vszfscjxn996Mg06K_bFmxLQAVQJmTLfwEX6avx</recordid><startdate>20160401</startdate><enddate>20160401</enddate><creator>Demont, Audrey, BS</creator><creator>Hacini-Rachinel, Feriel, PhD</creator><creator>Doucet-Ladevèze, Rémi, MS</creator><creator>Ngom-Bru, Catherine, PhD</creator><creator>Mercenier, Annick, PhD</creator><creator>Prioult, Guénolée, PhD</creator><creator>Blanchard, Carine, PhD</creator><general>Elsevier Inc</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7T5</scope><scope>H94</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-3377-290X</orcidid></search><sort><creationdate>20160401</creationdate><title>Live and heat-treated probiotics differently modulate IL10 mRNA stabilization and microRNA expression</title><author>Demont, Audrey, BS ; Hacini-Rachinel, Feriel, PhD ; Doucet-Ladevèze, Rémi, MS ; Ngom-Bru, Catherine, PhD ; Mercenier, Annick, PhD ; Prioult, Guénolée, PhD ; Blanchard, Carine, PhD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c483t-4d84b7318b0daa28f634d3b7ef7e65a095910220f0af4c00b56f10b56a7d23b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Allergy and Immunology</topic><topic>Biomarkers - metabolism</topic><topic>Cytokines</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>Hot Temperature</topic><topic>Humans</topic><topic>Interleukin-10 - genetics</topic><topic>Interleukin-10 - immunology</topic><topic>Lactobacillus - immunology</topic><topic>Lactobacillus - physiology</topic><topic>Leukocytes, Mononuclear - immunology</topic><topic>Leukocytes, Mononuclear - microbiology</topic><topic>Microorganisms</topic><topic>MicroRNAs</topic><topic>MicroRNAs - immunology</topic><topic>Physiology</topic><topic>Probiotics</topic><topic>Probiotics - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Demont, Audrey, BS</creatorcontrib><creatorcontrib>Hacini-Rachinel, Feriel, PhD</creatorcontrib><creatorcontrib>Doucet-Ladevèze, Rémi, MS</creatorcontrib><creatorcontrib>Ngom-Bru, Catherine, PhD</creatorcontrib><creatorcontrib>Mercenier, Annick, PhD</creatorcontrib><creatorcontrib>Prioult, Guénolée, PhD</creatorcontrib><creatorcontrib>Blanchard, Carine, PhD</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of allergy and clinical immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Demont, Audrey, BS</au><au>Hacini-Rachinel, Feriel, PhD</au><au>Doucet-Ladevèze, Rémi, MS</au><au>Ngom-Bru, Catherine, PhD</au><au>Mercenier, Annick, PhD</au><au>Prioult, Guénolée, PhD</au><au>Blanchard, Carine, PhD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Live and heat-treated probiotics differently modulate IL10 mRNA stabilization and microRNA expression</atitle><jtitle>Journal of allergy and clinical immunology</jtitle><addtitle>J Allergy Clin Immunol</addtitle><date>2016-04-01</date><risdate>2016</risdate><volume>137</volume><issue>4</issue><spage>1264</spage><epage>1267.e10</epage><pages>1264-1267.e10</pages><issn>0091-6749</issn><eissn>1097-6825</eissn><abstract>In inflammatory diseases and healthy animals, studies have shown a probiotic-induced IL-10 overproduction1 or an increase in regulatory FoxP3+ cell number.2 Interestingly, in vivo and in vitro studies have convincingly demonstrated differential activities of live and heat-treated organisms.3 Yet, little is known about the molecular mechanism involved in this modulation of IL10 mRNA expression. There are several AU-rich elements in the 3' untranslated region (UTR) of IL10 mRNA that have been shown to regulate IL10 gene expression level.4 PBMCs, transfected with a plasmid containing the IL10-3'UTR downstream of the luciferase (Luc) gene (Luc/IL10-3'UTR) and stimulated by heat-treated L paracasei NCC 2461 produced a significantly higher level of luciferase activity (3.6 ± 0.6 vs 0.6 ± 0.11; P < .05) than did transfected unstimulated cells (Fig 2, A), suggesting a role for IL10-3'UTR in heat-treated L paracasei NCC 2461-induced IL10 mRNA stabilization.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>26478009</pmid><doi>10.1016/j.jaci.2015.08.033</doi><orcidid>https://orcid.org/0000-0002-3377-290X</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Allergy and Immunology Biomarkers - metabolism Cytokines Gene expression Gene Expression Regulation Hot Temperature Humans Interleukin-10 - genetics Interleukin-10 - immunology Lactobacillus - immunology Lactobacillus - physiology Leukocytes, Mononuclear - immunology Leukocytes, Mononuclear - microbiology Microorganisms MicroRNAs MicroRNAs - immunology Physiology Probiotics Probiotics - pharmacology |
| title | Live and heat-treated probiotics differently modulate IL10 mRNA stabilization and microRNA expression |
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