Blastocysts derived from in vitro-fertilized cat oocytes after vitrification and dilution with sucrose
Experiments were conducted to find an optimal incubation period in a sucrose solution during dilution of cryoprotectants for obtaining a higher level of survival and development of cat oocytes cryopreserved by vitrification method. In the first experiment, in vitro-matured fresh oocytes were exposed...
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| Veröffentlicht in: | Cryobiology 2004-06, Vol.48 (3), p.341-348 |
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| creator | Murakami, Mk Otoi, T Karja, N.W.K Wongsrikeao, P Agung, B Suzuki, T |
| description | Experiments were conducted to find an optimal incubation period in a sucrose solution during dilution of cryoprotectants for obtaining a higher level of survival and development of cat oocytes cryopreserved by vitrification method. In the first experiment, in vitro-matured fresh oocytes were exposed to 0.5
M sucrose solution for 1 or 5
min before in vitro fertilization (IVF). The percentage of development to the blastocyst stage significantly decreased in oocytes exposed for 5
min, compared with oocytes exposed for 1
min and control oocytes without exposure to sucrose (
P |
| doi_str_mv | 10.1016/j.cryobiol.2004.02.012 |
| format | Article |
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M sucrose solution for 1 or 5
min before in vitro fertilization (IVF). The percentage of development to the blastocyst stage significantly decreased in oocytes exposed for 5
min, compared with oocytes exposed for 1
min and control oocytes without exposure to sucrose (
P<0.05). In the second experiment, oocytes that had been vitrified in 40% ethylene glycol and 0.3
M sucrose were liquefied and then incubated in 0.5
M sucrose for 0.5, 1 or 5
min to dilute the cryoprotectant. The percentage of cleavage (⩾2-cell stage) of vitrified–liquefied oocytes incubated for 0.5
min was significantly higher (
P<0.05) than that of other groups. Development of vitrified–liquefied oocytes to the morula and blastocyst stages after IVF was observed only in oocytes incubated in sucrose for 0.5
min. The present study indicates that the oocytes have sensitivity to the toxic effect of sucrose and that the incubation period during dilution of the cryoprotectant is of critical importance for developmental competence of vitrified–liquefied cat oocytes.</description><identifier>ISSN: 0011-2240</identifier><identifier>EISSN: 1090-2392</identifier><identifier>DOI: 10.1016/j.cryobiol.2004.02.012</identifier><identifier>PMID: 15157782</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Animals ; Blastocyst - cytology ; Blastocyst - ultrastructure ; Cats ; Cell Count ; Cell Survival - drug effects ; Cryopreservation ; Cryoprotective Agents - pharmacology ; Embryo development ; Ethylene Glycol - pharmacology ; Feline oocyte ; Fertilization in Vitro ; In vitro fertilization ; Male ; Oocytes ; Solutions ; Spermatozoa ; Sucrose - pharmacology ; Time Factors ; Vitrification</subject><ispartof>Cryobiology, 2004-06, Vol.48 (3), p.341-348</ispartof><rights>2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c310t-e3f5491bca2a84da631279e718521fad29c3f5d18a1bf92aa42cf65f680b52833</citedby><cites>FETCH-LOGICAL-c310t-e3f5491bca2a84da631279e718521fad29c3f5d18a1bf92aa42cf65f680b52833</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.cryobiol.2004.02.012$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15157782$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Murakami, Mk</creatorcontrib><creatorcontrib>Otoi, T</creatorcontrib><creatorcontrib>Karja, N.W.K</creatorcontrib><creatorcontrib>Wongsrikeao, P</creatorcontrib><creatorcontrib>Agung, B</creatorcontrib><creatorcontrib>Suzuki, T</creatorcontrib><title>Blastocysts derived from in vitro-fertilized cat oocytes after vitrification and dilution with sucrose</title><title>Cryobiology</title><addtitle>Cryobiology</addtitle><description>Experiments were conducted to find an optimal incubation period in a sucrose solution during dilution of cryoprotectants for obtaining a higher level of survival and development of cat oocytes cryopreserved by vitrification method. In the first experiment, in vitro-matured fresh oocytes were exposed to 0.5
M sucrose solution for 1 or 5
min before in vitro fertilization (IVF). The percentage of development to the blastocyst stage significantly decreased in oocytes exposed for 5
min, compared with oocytes exposed for 1
min and control oocytes without exposure to sucrose (
P<0.05). In the second experiment, oocytes that had been vitrified in 40% ethylene glycol and 0.3
M sucrose were liquefied and then incubated in 0.5
M sucrose for 0.5, 1 or 5
min to dilute the cryoprotectant. The percentage of cleavage (⩾2-cell stage) of vitrified–liquefied oocytes incubated for 0.5
min was significantly higher (
P<0.05) than that of other groups. Development of vitrified–liquefied oocytes to the morula and blastocyst stages after IVF was observed only in oocytes incubated in sucrose for 0.5
min. The present study indicates that the oocytes have sensitivity to the toxic effect of sucrose and that the incubation period during dilution of the cryoprotectant is of critical importance for developmental competence of vitrified–liquefied cat oocytes.</description><subject>Animals</subject><subject>Blastocyst - cytology</subject><subject>Blastocyst - ultrastructure</subject><subject>Cats</subject><subject>Cell Count</subject><subject>Cell Survival - drug effects</subject><subject>Cryopreservation</subject><subject>Cryoprotective Agents - pharmacology</subject><subject>Embryo development</subject><subject>Ethylene Glycol - pharmacology</subject><subject>Feline oocyte</subject><subject>Fertilization in Vitro</subject><subject>In vitro fertilization</subject><subject>Male</subject><subject>Oocytes</subject><subject>Solutions</subject><subject>Spermatozoa</subject><subject>Sucrose - pharmacology</subject><subject>Time Factors</subject><subject>Vitrification</subject><issn>0011-2240</issn><issn>1090-2392</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtv1DAQgC0EotvHX6h84pZ0xonzuAEVL6kSl3K2HHssvMrGxXYWLb--3u4ijpxGo_nm9TF2i1AjYHe3rU08hMmHuRYAbQ2iBhSv2AZhhEo0o3jNNgCIlRAtXLDLlLYA0PVN-5ZdoETZ94PYMPdx1ikHc0g5cUvR78lyF8OO-4XvfY6hchSzn_2fUjA681DgTIlrlym-IN75UvBh4Xqx3Pp5fUl--_yTp9XEkOiavXF6TnRzjlfsx-dPj_dfq4fvX77df3ioTIOQK2qcbEecjBZ6aK3uGhT9SD0OUqDTVoymEBYHjZMbhdatMK6TrhtgkmJomiv27jT3KYZfK6Wsdj4Zmme9UFiTwr4fpRxkAbsTeDwvRXLqKfqdjgeFoI6G1Vb9NayOhhUIVQyXxtvzhnXakf3XdlZagPcngMqfe09RJeNpMWR9JJOVDf5_O54Bl-GSqg</recordid><startdate>200406</startdate><enddate>200406</enddate><creator>Murakami, Mk</creator><creator>Otoi, T</creator><creator>Karja, N.W.K</creator><creator>Wongsrikeao, P</creator><creator>Agung, B</creator><creator>Suzuki, T</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>200406</creationdate><title>Blastocysts derived from in vitro-fertilized cat oocytes after vitrification and dilution with sucrose</title><author>Murakami, Mk ; Otoi, T ; Karja, N.W.K ; Wongsrikeao, P ; Agung, B ; Suzuki, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c310t-e3f5491bca2a84da631279e718521fad29c3f5d18a1bf92aa42cf65f680b52833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Blastocyst - cytology</topic><topic>Blastocyst - ultrastructure</topic><topic>Cats</topic><topic>Cell Count</topic><topic>Cell Survival - drug effects</topic><topic>Cryopreservation</topic><topic>Cryoprotective Agents - pharmacology</topic><topic>Embryo development</topic><topic>Ethylene Glycol - pharmacology</topic><topic>Feline oocyte</topic><topic>Fertilization in Vitro</topic><topic>In vitro fertilization</topic><topic>Male</topic><topic>Oocytes</topic><topic>Solutions</topic><topic>Spermatozoa</topic><topic>Sucrose - pharmacology</topic><topic>Time Factors</topic><topic>Vitrification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Murakami, Mk</creatorcontrib><creatorcontrib>Otoi, T</creatorcontrib><creatorcontrib>Karja, N.W.K</creatorcontrib><creatorcontrib>Wongsrikeao, P</creatorcontrib><creatorcontrib>Agung, B</creatorcontrib><creatorcontrib>Suzuki, T</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Cryobiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Murakami, Mk</au><au>Otoi, T</au><au>Karja, N.W.K</au><au>Wongsrikeao, P</au><au>Agung, B</au><au>Suzuki, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Blastocysts derived from in vitro-fertilized cat oocytes after vitrification and dilution with sucrose</atitle><jtitle>Cryobiology</jtitle><addtitle>Cryobiology</addtitle><date>2004-06</date><risdate>2004</risdate><volume>48</volume><issue>3</issue><spage>341</spage><epage>348</epage><pages>341-348</pages><issn>0011-2240</issn><eissn>1090-2392</eissn><abstract>Experiments were conducted to find an optimal incubation period in a sucrose solution during dilution of cryoprotectants for obtaining a higher level of survival and development of cat oocytes cryopreserved by vitrification method. In the first experiment, in vitro-matured fresh oocytes were exposed to 0.5
M sucrose solution for 1 or 5
min before in vitro fertilization (IVF). The percentage of development to the blastocyst stage significantly decreased in oocytes exposed for 5
min, compared with oocytes exposed for 1
min and control oocytes without exposure to sucrose (
P<0.05). In the second experiment, oocytes that had been vitrified in 40% ethylene glycol and 0.3
M sucrose were liquefied and then incubated in 0.5
M sucrose for 0.5, 1 or 5
min to dilute the cryoprotectant. The percentage of cleavage (⩾2-cell stage) of vitrified–liquefied oocytes incubated for 0.5
min was significantly higher (
P<0.05) than that of other groups. Development of vitrified–liquefied oocytes to the morula and blastocyst stages after IVF was observed only in oocytes incubated in sucrose for 0.5
min. The present study indicates that the oocytes have sensitivity to the toxic effect of sucrose and that the incubation period during dilution of the cryoprotectant is of critical importance for developmental competence of vitrified–liquefied cat oocytes.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>15157782</pmid><doi>10.1016/j.cryobiol.2004.02.012</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Blastocyst - cytology Blastocyst - ultrastructure Cats Cell Count Cell Survival - drug effects Cryopreservation Cryoprotective Agents - pharmacology Embryo development Ethylene Glycol - pharmacology Feline oocyte Fertilization in Vitro In vitro fertilization Male Oocytes Solutions Spermatozoa Sucrose - pharmacology Time Factors Vitrification |
| title | Blastocysts derived from in vitro-fertilized cat oocytes after vitrification and dilution with sucrose |
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