Characterization of recombinant forms of the yeast Gas1 protein and identification of residues essential for glucanosyltransferase activity and folding

Gas1p is a glycosylphosphatidylinositol‐anchored plasma membrane glycoprotein of Saccharomyces cerevisiae and is a representative of Family GH72 of glycosidases/transglycosidases, which also includes proteins from human fungal pathogens. Gas1p, Phr1‐2p from Candida albicans and Gel1p from Aspergillu...

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Veröffentlicht in:	European journal of biochemistry 2004-09, Vol.271 (18), p.3635-3645
Hauptverfasser:	Carotti, Cristina, Ragni, Enrico, Palomares, Oscar, Fontaine, Thierry, Tedeschi, Gabriella, Rodríguez, Rosalía, Latgé, Jean Paul, Vai, Marina, Popolo, Laura
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Amino Acid Substitution Aspergillus fumigatus Candida albicans Catalytic Domain Cysteine - metabolism Disulfides - analysis Disulfides - chemistry Fungal Proteins - metabolism Gas1 protein Gene Expression Genes, Fungal Glutamine - metabolism Glycosylphosphatidylinositols - chemistry Membrane Glycoproteins - chemistry Membrane Glycoproteins - metabolism Molecular Weight Mutagenesis, Site-Directed Pichia - genetics Pichia pastoris Protein Conformation Protein Folding Protein Structure, Tertiary Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - chemistry Saccharomyces cerevisiae Proteins - metabolism Solubility yeast cell wall β(1,3)‐glucanosyltransferase
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