Cryopreservation of sperm of the Pacific oyster ( Crassostrea gigas): development of a practical method for commercial spat production

This paper describes a simple method for cryopreserving sperm of the Pacific oyster ( Crassostrea gigas Thunberg) in quantities suitable for commercial spat production. Experiments to refine the cryoprotectant mixtures demonstrated the key role of trehalose. Trehalose alone (at 0.45 M final concentr...

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Veröffentlicht in:	Aquaculture 2004-12, Vol.242 (1), p.271-282
Hauptverfasser:	Adams, Serean L., Smith, John F., Roberts, Rodney D., Janke, Achim R., Kaspar, Heinrich F., Robin Tervit, H., Anne Pugh, P., Webb, Steven C., King, Nick G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal aquaculture animal fertility Animal productions Aquaculture Biological and medical sciences Commercial application Crassostrea gigas Cryopreservation cryoprotectants Fundamental and applied biological sciences. Psychology General aspects Hatchery mariculture Marine oyster culture Oysters Pacific oyster Reproductive technologies Sperm spermatozoa trehalose
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