The immunochemical detection of stress proteins in activated sludge exposed to toxic chemicals
The heat shock protein, GroEL, was found to be induced in activated sludge cultures exposed to perturbations of chemicals (cadmium, pentachlorophenol, and acetone) or heat stress. In laboratory activated sludge reactors, GroEL was rapidly induced (within minutes) in the presence of 5 mg/l or greater...
Gespeichert in:
| Veröffentlicht in: | Water research (Oxford) 2001, Vol.35 (1), p.91-100 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 100 |
|---|---|
| container_issue | 1 |
| container_start_page | 91 |
| container_title | Water research (Oxford) |
| container_volume | 35 |
| creator | Bott, Charles B Love, Nancy G |
| description | The heat shock protein, GroEL, was found to be induced in activated sludge cultures exposed to perturbations of chemicals (cadmium, pentachlorophenol, and acetone) or heat stress. In laboratory activated sludge reactors, GroEL was rapidly induced (within minutes) in the presence of 5 mg/l or greater total cadmium. At 5 mg/l cadmium, however, moderate to insignificant changes in activated sludge process performance indicators [effluent suspended solids concentration, chemical oxygen demand (COD) removal, and specific oxygen uptake rate] were observed. As total cadmium concentrations increased above 5 mg/l, there was a significant and consistent increase in effluent volatile suspended solids concentrations from activated sludge sequencing batch reactors relative to unstressed controls. These results indicate that stress proteins may serve as sensitive and rapid indicators of mixed liquor toxicity which can adversely impact treatment process performance, but that GroEL may not be a good candidate protein for this purpose. |
| doi_str_mv | 10.1016/S0043-1354(00)00245-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_17784758</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0043135400002451</els_id><sourcerecordid>14563305</sourcerecordid><originalsourceid>FETCH-LOGICAL-c452t-f856407ab854bf735397fc0006c95224638fde9ce0944e2538d3580310f877603</originalsourceid><addsrcrecordid>eNqFkV1rFTEQhoMo9nj0Jyi5ENGL1cnXJntVpPhRKHhhvTXsyU5s5OzmmNkt9d837Tna3hUGhiHPTF7el7GXAt4LEO2H7wBaNUIZ_RbgHYDUphGP2Eo42zVSa_eYrf4jR-wZ0W-olFTdU3YkhDTWdXbFfp5fIE_juEw5XOCYQr_lA84Y5pQnniOnuSAR35U8Y5qIp4n39fGyn3HgtF2GX8jxapepjnOudZUC_3eKnrMnsTZ8cehr9uPzp_OTr83Zty-nJx_PmqCNnJvoTKvB9htn9CZaZVRnY6h629AZKXWrXBywCwid1iiNcoMyDpSA6KxtQa3Zm_3dqvPPgjT7MVHA7bafMC_khbVOW-MeBrVplQJTQbMHQ8lEBaPflTT25a8X4G8S8LcJ-Bt7PYC_TaBOa_bq8MGyGXG42zpYXoHXB6CnalEs_RQS3eNAuhrTmh3vMay2XSYsnkLCKeCQSk3HDzk9oOQaJ8uhZg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>14563305</pqid></control><display><type>article</type><title>The immunochemical detection of stress proteins in activated sludge exposed to toxic chemicals</title><source>MEDLINE</source><source>ScienceDirect Freedom Collection (Elsevier)</source><creator>Bott, Charles B ; Love, Nancy G</creator><creatorcontrib>Bott, Charles B ; Love, Nancy G</creatorcontrib><description>The heat shock protein, GroEL, was found to be induced in activated sludge cultures exposed to perturbations of chemicals (cadmium, pentachlorophenol, and acetone) or heat stress. In laboratory activated sludge reactors, GroEL was rapidly induced (within minutes) in the presence of 5 mg/l or greater total cadmium. At 5 mg/l cadmium, however, moderate to insignificant changes in activated sludge process performance indicators [effluent suspended solids concentration, chemical oxygen demand (COD) removal, and specific oxygen uptake rate] were observed. As total cadmium concentrations increased above 5 mg/l, there was a significant and consistent increase in effluent volatile suspended solids concentrations from activated sludge sequencing batch reactors relative to unstressed controls. These results indicate that stress proteins may serve as sensitive and rapid indicators of mixed liquor toxicity which can adversely impact treatment process performance, but that GroEL may not be a good candidate protein for this purpose.</description><identifier>ISSN: 0043-1354</identifier><identifier>EISSN: 1879-2448</identifier><identifier>DOI: 10.1016/S0043-1354(00)00245-1</identifier><identifier>PMID: 11257897</identifier><identifier>CODEN: WATRAG</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>acetone ; Applied sciences ; Biological and medical sciences ; Biological treatment of waters ; Biotechnology ; Blotting, Western - methods ; Cadmium - analysis ; Cadmium - isolation & purification ; Chaperonin 60 - analysis ; Environment and pollution ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; General purification processes ; GroEL ; Heat-Shock Proteins - analysis ; Hsp60 ; Industrial applications and implications. Economical aspects ; pentachlorophenol ; Pollution ; protein ; Sensitivity and Specificity ; Sewage - analysis ; shock ; stress ; Wastewaters ; Water Pollutants, Chemical - analysis ; Water Pollution, Chemical ; Water treatment and pollution ; xenobiotic</subject><ispartof>Water research (Oxford), 2001, Vol.35 (1), p.91-100</ispartof><rights>2000 Elsevier Science Ltd</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-f856407ab854bf735397fc0006c95224638fde9ce0944e2538d3580310f877603</citedby><cites>FETCH-LOGICAL-c452t-f856407ab854bf735397fc0006c95224638fde9ce0944e2538d3580310f877603</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0043-1354(00)00245-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1102822$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11257897$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bott, Charles B</creatorcontrib><creatorcontrib>Love, Nancy G</creatorcontrib><title>The immunochemical detection of stress proteins in activated sludge exposed to toxic chemicals</title><title>Water research (Oxford)</title><addtitle>Water Res</addtitle><description>The heat shock protein, GroEL, was found to be induced in activated sludge cultures exposed to perturbations of chemicals (cadmium, pentachlorophenol, and acetone) or heat stress. In laboratory activated sludge reactors, GroEL was rapidly induced (within minutes) in the presence of 5 mg/l or greater total cadmium. At 5 mg/l cadmium, however, moderate to insignificant changes in activated sludge process performance indicators [effluent suspended solids concentration, chemical oxygen demand (COD) removal, and specific oxygen uptake rate] were observed. As total cadmium concentrations increased above 5 mg/l, there was a significant and consistent increase in effluent volatile suspended solids concentrations from activated sludge sequencing batch reactors relative to unstressed controls. These results indicate that stress proteins may serve as sensitive and rapid indicators of mixed liquor toxicity which can adversely impact treatment process performance, but that GroEL may not be a good candidate protein for this purpose.</description><subject>acetone</subject><subject>Applied sciences</subject><subject>Biological and medical sciences</subject><subject>Biological treatment of waters</subject><subject>Biotechnology</subject><subject>Blotting, Western - methods</subject><subject>Cadmium - analysis</subject><subject>Cadmium - isolation & purification</subject><subject>Chaperonin 60 - analysis</subject><subject>Environment and pollution</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General purification processes</subject><subject>GroEL</subject><subject>Heat-Shock Proteins - analysis</subject><subject>Hsp60</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>pentachlorophenol</subject><subject>Pollution</subject><subject>protein</subject><subject>Sensitivity and Specificity</subject><subject>Sewage - analysis</subject><subject>shock</subject><subject>stress</subject><subject>Wastewaters</subject><subject>Water Pollutants, Chemical - analysis</subject><subject>Water Pollution, Chemical</subject><subject>Water treatment and pollution</subject><subject>xenobiotic</subject><issn>0043-1354</issn><issn>1879-2448</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV1rFTEQhoMo9nj0Jyi5ENGL1cnXJntVpPhRKHhhvTXsyU5s5OzmmNkt9d837Tna3hUGhiHPTF7el7GXAt4LEO2H7wBaNUIZ_RbgHYDUphGP2Eo42zVSa_eYrf4jR-wZ0W-olFTdU3YkhDTWdXbFfp5fIE_juEw5XOCYQr_lA84Y5pQnniOnuSAR35U8Y5qIp4n39fGyn3HgtF2GX8jxapepjnOudZUC_3eKnrMnsTZ8cehr9uPzp_OTr83Zty-nJx_PmqCNnJvoTKvB9htn9CZaZVRnY6h629AZKXWrXBywCwid1iiNcoMyDpSA6KxtQa3Zm_3dqvPPgjT7MVHA7bafMC_khbVOW-MeBrVplQJTQbMHQ8lEBaPflTT25a8X4G8S8LcJ-Bt7PYC_TaBOa_bq8MGyGXG42zpYXoHXB6CnalEs_RQS3eNAuhrTmh3vMay2XSYsnkLCKeCQSk3HDzk9oOQaJ8uhZg</recordid><startdate>2001</startdate><enddate>2001</enddate><creator>Bott, Charles B</creator><creator>Love, Nancy G</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>C1K</scope><scope>SOI</scope><scope>7QH</scope><scope>7TV</scope><scope>7U7</scope><scope>7UA</scope></search><sort><creationdate>2001</creationdate><title>The immunochemical detection of stress proteins in activated sludge exposed to toxic chemicals</title><author>Bott, Charles B ; Love, Nancy G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-f856407ab854bf735397fc0006c95224638fde9ce0944e2538d3580310f877603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>acetone</topic><topic>Applied sciences</topic><topic>Biological and medical sciences</topic><topic>Biological treatment of waters</topic><topic>Biotechnology</topic><topic>Blotting, Western - methods</topic><topic>Cadmium - analysis</topic><topic>Cadmium - isolation & purification</topic><topic>Chaperonin 60 - analysis</topic><topic>Environment and pollution</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General purification processes</topic><topic>GroEL</topic><topic>Heat-Shock Proteins - analysis</topic><topic>Hsp60</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>pentachlorophenol</topic><topic>Pollution</topic><topic>protein</topic><topic>Sensitivity and Specificity</topic><topic>Sewage - analysis</topic><topic>shock</topic><topic>stress</topic><topic>Wastewaters</topic><topic>Water Pollutants, Chemical - analysis</topic><topic>Water Pollution, Chemical</topic><topic>Water treatment and pollution</topic><topic>xenobiotic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bott, Charles B</creatorcontrib><creatorcontrib>Love, Nancy G</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><collection>Aqualine</collection><collection>Pollution Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><jtitle>Water research (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bott, Charles B</au><au>Love, Nancy G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The immunochemical detection of stress proteins in activated sludge exposed to toxic chemicals</atitle><jtitle>Water research (Oxford)</jtitle><addtitle>Water Res</addtitle><date>2001</date><risdate>2001</risdate><volume>35</volume><issue>1</issue><spage>91</spage><epage>100</epage><pages>91-100</pages><issn>0043-1354</issn><eissn>1879-2448</eissn><coden>WATRAG</coden><abstract>The heat shock protein, GroEL, was found to be induced in activated sludge cultures exposed to perturbations of chemicals (cadmium, pentachlorophenol, and acetone) or heat stress. In laboratory activated sludge reactors, GroEL was rapidly induced (within minutes) in the presence of 5 mg/l or greater total cadmium. At 5 mg/l cadmium, however, moderate to insignificant changes in activated sludge process performance indicators [effluent suspended solids concentration, chemical oxygen demand (COD) removal, and specific oxygen uptake rate] were observed. As total cadmium concentrations increased above 5 mg/l, there was a significant and consistent increase in effluent volatile suspended solids concentrations from activated sludge sequencing batch reactors relative to unstressed controls. These results indicate that stress proteins may serve as sensitive and rapid indicators of mixed liquor toxicity which can adversely impact treatment process performance, but that GroEL may not be a good candidate protein for this purpose.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>11257897</pmid><doi>10.1016/S0043-1354(00)00245-1</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0043-1354 |
| ispartof | Water research (Oxford), 2001, Vol.35 (1), p.91-100 |
| issn | 0043-1354 1879-2448 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17784758 |
| source | MEDLINE; ScienceDirect Freedom Collection (Elsevier) |
| subjects | acetone Applied sciences Biological and medical sciences Biological treatment of waters Biotechnology Blotting, Western - methods Cadmium - analysis Cadmium - isolation & purification Chaperonin 60 - analysis Environment and pollution Exact sciences and technology Fundamental and applied biological sciences. Psychology General purification processes GroEL Heat-Shock Proteins - analysis Hsp60 Industrial applications and implications. Economical aspects pentachlorophenol Pollution protein Sensitivity and Specificity Sewage - analysis shock stress Wastewaters Water Pollutants, Chemical - analysis Water Pollution, Chemical Water treatment and pollution xenobiotic |
| title | The immunochemical detection of stress proteins in activated sludge exposed to toxic chemicals |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T07%3A26%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20immunochemical%20detection%20of%20stress%20proteins%20in%20activated%20sludge%20exposed%20to%20toxic%20chemicals&rft.jtitle=Water%20research%20(Oxford)&rft.au=Bott,%20Charles%20B&rft.date=2001&rft.volume=35&rft.issue=1&rft.spage=91&rft.epage=100&rft.pages=91-100&rft.issn=0043-1354&rft.eissn=1879-2448&rft.coden=WATRAG&rft_id=info:doi/10.1016/S0043-1354(00)00245-1&rft_dat=%3Cproquest_cross%3E14563305%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=14563305&rft_id=info:pmid/11257897&rft_els_id=S0043135400002451&rfr_iscdi=true |