Antibiotic resistance marker genes as environmental pollutants in GMO-pristine agricultural soils in Austria

Antibiotic resistance genes may be considered as environmental pollutants if anthropogenic emission and manipulations increase their prevalence above usually occurring background levels. The prevalence of aph(3′)-IIa/nptII and aph(3′)-IIIa/nptIII – frequent marker genes in plant biotechnology conferring resistance to certain aminoglycosides – was determined in Austrian soils from 100 maize and potato fields not yet exposed to but eligible for GMO crop cultivation. Total soil DNA extracts were analysed by nptII/nptIII-specific TaqMan real time PCR. Of all fields 6% were positive for nptII (median: 150 copies/g soil; range: 31–856) and 85% for nptIII (1190 copies/g soil; 13–61600). The copy-number deduced prevalence of nptIII carriers was 14-fold higher compared to nptII. Of the cultivable kanamycin-resistant soil bacteria 1.8% (95% confidence interval: 0–3.3%) were positive for nptIII, none for nptII (0–0.8%). The nptII-load of the studied soils was low rendering nptII a typical candidate as environmental pollutant upon anthropogenic release into these ecosystems. •ARM genes may act as environmental pollutants under certain conditions.•Vital criteria for rating are low endemic presence and anthropogenic ARG immission.•Agricultural soils were rarely positive for nptII with few gene copy numbers.•Most fields were nptIII positive with variable but also increased allele frequency.•NptII/III qualify as pollutants in the tested settings with low endemic abundances. ARM genes may be considered as environmental pollutants if anthropogenic activities raise their abundance above naturally occurring background levels in exposed ecosystems.