Effect of bioemulsificant exopolysaccharide (EPS2003) on microbial community dynamics during assays of oil spill bioremediation: A microcosm study
► EPS2003 can be applied as an oil spill remediation agent in marine environments. ► Presence of EPS2003 influence the quali-, quantitative composition of marine bacteria. ► EPS2003 favors the increment of hydrocarbonoclastic bacteria (e.g. Alcanivorax sp.). Microcosms experiments were carried out t...
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| Veröffentlicht in: | Marine pollution bulletin 2012-12, Vol.64 (12), p.2820-2828 |
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| creator | Cappello, Simone Genovese, Maria Della Torre, Camilla Crisari, Antonella Hassanshahian, Mehdi Santisi, Santina Calogero, Rosario Yakimov, Michail M. |
| description | ► EPS2003 can be applied as an oil spill remediation agent in marine environments. ► Presence of EPS2003 influence the quali-, quantitative composition of marine bacteria. ► EPS2003 favors the increment of hydrocarbonoclastic bacteria (e.g. Alcanivorax sp.).
Microcosms experiments were carried out to evaluate the effect of bioemulsificant exopolysaccharide (EPS2003) on microbial community dynamics. An experimental seawater microcosm, supplemented with crude oil and EPS2003 (SW+OIL+EPS2003), was monitored for 15days and compared to control microcosm (only oil-polluted seawater, SW+OIL). Determination of bacterial abundance, heterotrophic cultivable and hydrocarbon-degrading bacteria were carried out during all experimentation period. The microbial community dynamic was monitored by isolation of total RNA, RT-PCR amplification of 16S rRNA, cloning and sequencing. Oil degradation was monitored by GC–MS analysis. Bioemulsificant addition stimulated an increase of the total bacterial abundance, change in the community structure and activity. The bioemulsificant also increased of 5 times the oil biodegradation rate.
The data obtained from microcosm experiment indicated that EPS2003 could be used for the dispersion of oil slicks and could stimulate the selection of marine hydrocarbon degraders thus increasing bioremediation process. |
| doi_str_mv | 10.1016/j.marpolbul.2012.07.046 |
| format | Article |
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Microcosms experiments were carried out to evaluate the effect of bioemulsificant exopolysaccharide (EPS2003) on microbial community dynamics. An experimental seawater microcosm, supplemented with crude oil and EPS2003 (SW+OIL+EPS2003), was monitored for 15days and compared to control microcosm (only oil-polluted seawater, SW+OIL). Determination of bacterial abundance, heterotrophic cultivable and hydrocarbon-degrading bacteria were carried out during all experimentation period. The microbial community dynamic was monitored by isolation of total RNA, RT-PCR amplification of 16S rRNA, cloning and sequencing. Oil degradation was monitored by GC–MS analysis. Bioemulsificant addition stimulated an increase of the total bacterial abundance, change in the community structure and activity. The bioemulsificant also increased of 5 times the oil biodegradation rate.
The data obtained from microcosm experiment indicated that EPS2003 could be used for the dispersion of oil slicks and could stimulate the selection of marine hydrocarbon degraders thus increasing bioremediation process.</description><identifier>ISSN: 0025-326X</identifier><identifier>EISSN: 1879-3363</identifier><identifier>DOI: 10.1016/j.marpolbul.2012.07.046</identifier><identifier>PMID: 23067540</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Alcanivorax ; assays ; Bacteria ; Bacteria - classification ; Bacteria - drug effects ; Bacteria - metabolism ; biodegradation ; Biodegradation, Environmental ; Bioemulsificant ; Bioremediation ; Colony Count, Microbial ; Communities ; community structure ; Degradation ; Dynamic tests ; Dynamics ; Ecosystem ; Emulsifying Agents - toxicity ; exopolysaccharides ; gas chromatography-mass spectrometry ; Heterotrophic Processes ; Hydrocarbons - analysis ; Hydrocarbons - metabolism ; Marine ; microbial communities ; Microbial diversity ; Microcosms ; Microorganisms ; Oil spill ; oil spills ; oils ; petroleum ; Petroleum - analysis ; Petroleum - metabolism ; Petroleum Pollution ; Polysaccharides - toxicity ; reverse transcriptase polymerase chain reaction ; ribosomal RNA ; Sea water ; seawater ; Seawater - chemistry ; Seawater - microbiology ; Water Microbiology ; Water Pollutants, Chemical - toxicity ; water pollution</subject><ispartof>Marine pollution bulletin, 2012-12, Vol.64 (12), p.2820-2828</ispartof><rights>2012 Elsevier Ltd</rights><rights>Copyright © 2012 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c461t-8d848e23a3e73d802fc092bdd9b4201710edb6b23095821ebac6d3e2b2a832f03</citedby><cites>FETCH-LOGICAL-c461t-8d848e23a3e73d802fc092bdd9b4201710edb6b23095821ebac6d3e2b2a832f03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.marpolbul.2012.07.046$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23067540$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cappello, Simone</creatorcontrib><creatorcontrib>Genovese, Maria</creatorcontrib><creatorcontrib>Della Torre, Camilla</creatorcontrib><creatorcontrib>Crisari, Antonella</creatorcontrib><creatorcontrib>Hassanshahian, Mehdi</creatorcontrib><creatorcontrib>Santisi, Santina</creatorcontrib><creatorcontrib>Calogero, Rosario</creatorcontrib><creatorcontrib>Yakimov, Michail M.</creatorcontrib><title>Effect of bioemulsificant exopolysaccharide (EPS2003) on microbial community dynamics during assays of oil spill bioremediation: A microcosm study</title><title>Marine pollution bulletin</title><addtitle>Mar Pollut Bull</addtitle><description>► EPS2003 can be applied as an oil spill remediation agent in marine environments. ► Presence of EPS2003 influence the quali-, quantitative composition of marine bacteria. ► EPS2003 favors the increment of hydrocarbonoclastic bacteria (e.g. Alcanivorax sp.).
Microcosms experiments were carried out to evaluate the effect of bioemulsificant exopolysaccharide (EPS2003) on microbial community dynamics. An experimental seawater microcosm, supplemented with crude oil and EPS2003 (SW+OIL+EPS2003), was monitored for 15days and compared to control microcosm (only oil-polluted seawater, SW+OIL). Determination of bacterial abundance, heterotrophic cultivable and hydrocarbon-degrading bacteria were carried out during all experimentation period. The microbial community dynamic was monitored by isolation of total RNA, RT-PCR amplification of 16S rRNA, cloning and sequencing. Oil degradation was monitored by GC–MS analysis. Bioemulsificant addition stimulated an increase of the total bacterial abundance, change in the community structure and activity. The bioemulsificant also increased of 5 times the oil biodegradation rate.
The data obtained from microcosm experiment indicated that EPS2003 could be used for the dispersion of oil slicks and could stimulate the selection of marine hydrocarbon degraders thus increasing bioremediation process.</description><subject>Alcanivorax</subject><subject>assays</subject><subject>Bacteria</subject><subject>Bacteria - classification</subject><subject>Bacteria - drug effects</subject><subject>Bacteria - metabolism</subject><subject>biodegradation</subject><subject>Biodegradation, Environmental</subject><subject>Bioemulsificant</subject><subject>Bioremediation</subject><subject>Colony Count, Microbial</subject><subject>Communities</subject><subject>community structure</subject><subject>Degradation</subject><subject>Dynamic tests</subject><subject>Dynamics</subject><subject>Ecosystem</subject><subject>Emulsifying Agents - toxicity</subject><subject>exopolysaccharides</subject><subject>gas chromatography-mass spectrometry</subject><subject>Heterotrophic Processes</subject><subject>Hydrocarbons - analysis</subject><subject>Hydrocarbons - metabolism</subject><subject>Marine</subject><subject>microbial communities</subject><subject>Microbial diversity</subject><subject>Microcosms</subject><subject>Microorganisms</subject><subject>Oil spill</subject><subject>oil spills</subject><subject>oils</subject><subject>petroleum</subject><subject>Petroleum - analysis</subject><subject>Petroleum - metabolism</subject><subject>Petroleum Pollution</subject><subject>Polysaccharides - toxicity</subject><subject>reverse transcriptase polymerase chain reaction</subject><subject>ribosomal RNA</subject><subject>Sea water</subject><subject>seawater</subject><subject>Seawater - chemistry</subject><subject>Seawater - microbiology</subject><subject>Water Microbiology</subject><subject>Water Pollutants, Chemical - toxicity</subject><subject>water pollution</subject><issn>0025-326X</issn><issn>1879-3363</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkstu1DAUQC0EokPhF6iXZZFwbWfshN2oGgpSJZCgEjvLrxSPnHiwE0R-gy-uoyndlo0tWec-jxG6IFATIPz9oR5UOsag51BTILQGUUPDn6ENaUVXMcbZc7QBoNuKUf7jDL3K-QAAggryEp1RBlxsG9igv_u-d2bCscfaRzfMIfveGzVO2P2JpcKSlTE_VfLW4cv9128UgL3DccSDNylqrwI2cRjm0U8LtsuoynvGdk5-vMMqZ7XkNXn0AeejD2Etk9zgrFeTj-MHvDtlMjEPOE-zXV6jF70K2b15uM_R7cf996tP1c2X689Xu5vKNJxMVWvbpnWUKeYEsy3Q3kBHtbWdbspKBAFnNddl1G7bUuK0MtwyRzVVLaM9sHN0ecp7TPHX7PIkB5-NC0GNLs5ZEiFaYOVsnkZpRzhAQ9h_oBQY33aNKKg4oWX8nJPr5TH54nWRBOSqWR7ko2a5apYgZNFcIt8-FJl1WeVj3D-vBbg4Ab2KUt0ln-VtUbc2CYSUNguxOxGurPi3d0lm491oiphUPoS00T_Zxj1EbsfX</recordid><startdate>20121201</startdate><enddate>20121201</enddate><creator>Cappello, Simone</creator><creator>Genovese, Maria</creator><creator>Della Torre, Camilla</creator><creator>Crisari, Antonella</creator><creator>Hassanshahian, Mehdi</creator><creator>Santisi, Santina</creator><creator>Calogero, Rosario</creator><creator>Yakimov, Michail M.</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>7ST</scope><scope>7T7</scope><scope>7TN</scope><scope>7TV</scope><scope>7UA</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H97</scope><scope>L.G</scope><scope>P64</scope><scope>SOI</scope><scope>KR7</scope></search><sort><creationdate>20121201</creationdate><title>Effect of bioemulsificant exopolysaccharide (EPS2003) on microbial community dynamics during assays of oil spill bioremediation: A microcosm study</title><author>Cappello, Simone ; Genovese, Maria ; Della Torre, Camilla ; Crisari, Antonella ; Hassanshahian, Mehdi ; Santisi, Santina ; Calogero, Rosario ; Yakimov, Michail M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c461t-8d848e23a3e73d802fc092bdd9b4201710edb6b23095821ebac6d3e2b2a832f03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Alcanivorax</topic><topic>assays</topic><topic>Bacteria</topic><topic>Bacteria - classification</topic><topic>Bacteria - drug effects</topic><topic>Bacteria - metabolism</topic><topic>biodegradation</topic><topic>Biodegradation, Environmental</topic><topic>Bioemulsificant</topic><topic>Bioremediation</topic><topic>Colony Count, Microbial</topic><topic>Communities</topic><topic>community structure</topic><topic>Degradation</topic><topic>Dynamic tests</topic><topic>Dynamics</topic><topic>Ecosystem</topic><topic>Emulsifying Agents - toxicity</topic><topic>exopolysaccharides</topic><topic>gas chromatography-mass spectrometry</topic><topic>Heterotrophic Processes</topic><topic>Hydrocarbons - analysis</topic><topic>Hydrocarbons - metabolism</topic><topic>Marine</topic><topic>microbial communities</topic><topic>Microbial diversity</topic><topic>Microcosms</topic><topic>Microorganisms</topic><topic>Oil spill</topic><topic>oil spills</topic><topic>oils</topic><topic>petroleum</topic><topic>Petroleum - analysis</topic><topic>Petroleum - metabolism</topic><topic>Petroleum Pollution</topic><topic>Polysaccharides - toxicity</topic><topic>reverse transcriptase polymerase chain reaction</topic><topic>ribosomal RNA</topic><topic>Sea water</topic><topic>seawater</topic><topic>Seawater - chemistry</topic><topic>Seawater - microbiology</topic><topic>Water Microbiology</topic><topic>Water Pollutants, Chemical - toxicity</topic><topic>water pollution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cappello, Simone</creatorcontrib><creatorcontrib>Genovese, Maria</creatorcontrib><creatorcontrib>Della Torre, Camilla</creatorcontrib><creatorcontrib>Crisari, Antonella</creatorcontrib><creatorcontrib>Hassanshahian, Mehdi</creatorcontrib><creatorcontrib>Santisi, Santina</creatorcontrib><creatorcontrib>Calogero, Rosario</creatorcontrib><creatorcontrib>Yakimov, Michail M.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Oceanic Abstracts</collection><collection>Pollution Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>Civil Engineering Abstracts</collection><jtitle>Marine pollution bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cappello, Simone</au><au>Genovese, Maria</au><au>Della Torre, Camilla</au><au>Crisari, Antonella</au><au>Hassanshahian, Mehdi</au><au>Santisi, Santina</au><au>Calogero, Rosario</au><au>Yakimov, Michail M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of bioemulsificant exopolysaccharide (EPS2003) on microbial community dynamics during assays of oil spill bioremediation: A microcosm study</atitle><jtitle>Marine pollution bulletin</jtitle><addtitle>Mar Pollut Bull</addtitle><date>2012-12-01</date><risdate>2012</risdate><volume>64</volume><issue>12</issue><spage>2820</spage><epage>2828</epage><pages>2820-2828</pages><issn>0025-326X</issn><eissn>1879-3363</eissn><abstract>► EPS2003 can be applied as an oil spill remediation agent in marine environments. ► Presence of EPS2003 influence the quali-, quantitative composition of marine bacteria. ► EPS2003 favors the increment of hydrocarbonoclastic bacteria (e.g. Alcanivorax sp.).
Microcosms experiments were carried out to evaluate the effect of bioemulsificant exopolysaccharide (EPS2003) on microbial community dynamics. An experimental seawater microcosm, supplemented with crude oil and EPS2003 (SW+OIL+EPS2003), was monitored for 15days and compared to control microcosm (only oil-polluted seawater, SW+OIL). Determination of bacterial abundance, heterotrophic cultivable and hydrocarbon-degrading bacteria were carried out during all experimentation period. The microbial community dynamic was monitored by isolation of total RNA, RT-PCR amplification of 16S rRNA, cloning and sequencing. Oil degradation was monitored by GC–MS analysis. Bioemulsificant addition stimulated an increase of the total bacterial abundance, change in the community structure and activity. The bioemulsificant also increased of 5 times the oil biodegradation rate.
The data obtained from microcosm experiment indicated that EPS2003 could be used for the dispersion of oil slicks and could stimulate the selection of marine hydrocarbon degraders thus increasing bioremediation process.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>23067540</pmid><doi>10.1016/j.marpolbul.2012.07.046</doi><tpages>9</tpages></addata></record> |
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| subjects | Alcanivorax assays Bacteria Bacteria - classification Bacteria - drug effects Bacteria - metabolism biodegradation Biodegradation, Environmental Bioemulsificant Bioremediation Colony Count, Microbial Communities community structure Degradation Dynamic tests Dynamics Ecosystem Emulsifying Agents - toxicity exopolysaccharides gas chromatography-mass spectrometry Heterotrophic Processes Hydrocarbons - analysis Hydrocarbons - metabolism Marine microbial communities Microbial diversity Microcosms Microorganisms Oil spill oil spills oils petroleum Petroleum - analysis Petroleum - metabolism Petroleum Pollution Polysaccharides - toxicity reverse transcriptase polymerase chain reaction ribosomal RNA Sea water seawater Seawater - chemistry Seawater - microbiology Water Microbiology Water Pollutants, Chemical - toxicity water pollution |
| title | Effect of bioemulsificant exopolysaccharide (EPS2003) on microbial community dynamics during assays of oil spill bioremediation: A microcosm study |
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