Advances in affinity ligand-functionalized nanomaterials for biomagnetic separation
ABSTRACT The downstream processing of proteins remains the most significant cost in protein production, and is largely attributed to rigorous chromatographic purification protocols, where the stringency of purity for biopharmaceutical products sometimes exceeds 99%. With an ever burgeoning biotechno...
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| Veröffentlicht in: | Biotechnology and bioengineering 2016-01, Vol.113 (1), p.11-25 |
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| creator | Fields, Conor Li, Peng O'Mahony, James J. Lee, Gil U. |
| description | ABSTRACT
The downstream processing of proteins remains the most significant cost in protein production, and is largely attributed to rigorous chromatographic purification protocols, where the stringency of purity for biopharmaceutical products sometimes exceeds 99%. With an ever burgeoning biotechnology market, there is a constant demand for alternative purification methodologies, to ameliorate the dependence on chromatography, while still adhering to regulatory concerns over product purity and safety. In this article, we present an up‐to‐date view of bioseparation, with emphasis on magnetic separation and its potential application in the field. Additionally, we discuss the economic and performance benefits of synthetic ligands, in the form of peptides and miniaturized antibody fragments, compared to full‐length antibodies. We propose that adoption of synthetic affinity ligands coupled with magnetic adsorbents, will play an important role in enabling sustainable bioprocessing in the future. Biotechnol. Bioeng. 2016;113: 11–25. © 2015 Wiley Periodicals, Inc.
Magnetic separation, in combination with adoption of synthetic affinity ligands, offers an alternative approach to protein purification and has the potential to alleviate bottleneck issues associated with industrial‐scale downstream chromatographic purification. This review article focuses on magnetic bioseparation, with emphasis on the economic and performance benefits of peptides and antibody fragments, as alternative, synthetic affinity ligand reagents to full‐length antibodies. |
| doi_str_mv | 10.1002/bit.25665 |
| format | Article |
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The downstream processing of proteins remains the most significant cost in protein production, and is largely attributed to rigorous chromatographic purification protocols, where the stringency of purity for biopharmaceutical products sometimes exceeds 99%. With an ever burgeoning biotechnology market, there is a constant demand for alternative purification methodologies, to ameliorate the dependence on chromatography, while still adhering to regulatory concerns over product purity and safety. In this article, we present an up‐to‐date view of bioseparation, with emphasis on magnetic separation and its potential application in the field. Additionally, we discuss the economic and performance benefits of synthetic ligands, in the form of peptides and miniaturized antibody fragments, compared to full‐length antibodies. We propose that adoption of synthetic affinity ligands coupled with magnetic adsorbents, will play an important role in enabling sustainable bioprocessing in the future. Biotechnol. Bioeng. 2016;113: 11–25. © 2015 Wiley Periodicals, Inc.
Magnetic separation, in combination with adoption of synthetic affinity ligands, offers an alternative approach to protein purification and has the potential to alleviate bottleneck issues associated with industrial‐scale downstream chromatographic purification. This review article focuses on magnetic bioseparation, with emphasis on the economic and performance benefits of peptides and antibody fragments, as alternative, synthetic affinity ligand reagents to full‐length antibodies.</description><identifier>ISSN: 0006-3592</identifier><identifier>EISSN: 1097-0290</identifier><identifier>DOI: 10.1002/bit.25665</identifier><identifier>PMID: 26032605</identifier><identifier>CODEN: BIBIAU</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Affinity ; Antibodies ; antibody fragments ; bioseparation ; Biotechnology ; Biotechnology - methods ; Chromatography ; Economics ; Fragments ; Ligands ; Membrane separation ; micro-particles ; Molecules ; Nanomaterials ; nanoparticles ; Nanostructures ; Peptides ; Proteins ; Purification ; Recombinant Proteins - isolation & purification ; superparamagnetic ; Technology, Pharmaceutical - methods</subject><ispartof>Biotechnology and bioengineering, 2016-01, Vol.113 (1), p.11-25</ispartof><rights>2015 Wiley Periodicals, Inc.</rights><rights>Copyright Wiley Subscription Services, Inc. Jan 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5295-916629f34526e721d68b2dfc0ec5d81506c1945b0b474023c6b44d991614cc133</citedby><cites>FETCH-LOGICAL-c5295-916629f34526e721d68b2dfc0ec5d81506c1945b0b474023c6b44d991614cc133</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbit.25665$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbit.25665$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26032605$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fields, Conor</creatorcontrib><creatorcontrib>Li, Peng</creatorcontrib><creatorcontrib>O'Mahony, James J.</creatorcontrib><creatorcontrib>Lee, Gil U.</creatorcontrib><title>Advances in affinity ligand-functionalized nanomaterials for biomagnetic separation</title><title>Biotechnology and bioengineering</title><addtitle>Biotechnol. Bioeng</addtitle><description>ABSTRACT
The downstream processing of proteins remains the most significant cost in protein production, and is largely attributed to rigorous chromatographic purification protocols, where the stringency of purity for biopharmaceutical products sometimes exceeds 99%. With an ever burgeoning biotechnology market, there is a constant demand for alternative purification methodologies, to ameliorate the dependence on chromatography, while still adhering to regulatory concerns over product purity and safety. In this article, we present an up‐to‐date view of bioseparation, with emphasis on magnetic separation and its potential application in the field. Additionally, we discuss the economic and performance benefits of synthetic ligands, in the form of peptides and miniaturized antibody fragments, compared to full‐length antibodies. We propose that adoption of synthetic affinity ligands coupled with magnetic adsorbents, will play an important role in enabling sustainable bioprocessing in the future. Biotechnol. Bioeng. 2016;113: 11–25. © 2015 Wiley Periodicals, Inc.
Magnetic separation, in combination with adoption of synthetic affinity ligands, offers an alternative approach to protein purification and has the potential to alleviate bottleneck issues associated with industrial‐scale downstream chromatographic purification. This review article focuses on magnetic bioseparation, with emphasis on the economic and performance benefits of peptides and antibody fragments, as alternative, synthetic affinity ligand reagents to full‐length antibodies.</description><subject>Affinity</subject><subject>Antibodies</subject><subject>antibody fragments</subject><subject>bioseparation</subject><subject>Biotechnology</subject><subject>Biotechnology - methods</subject><subject>Chromatography</subject><subject>Economics</subject><subject>Fragments</subject><subject>Ligands</subject><subject>Membrane separation</subject><subject>micro-particles</subject><subject>Molecules</subject><subject>Nanomaterials</subject><subject>nanoparticles</subject><subject>Nanostructures</subject><subject>Peptides</subject><subject>Proteins</subject><subject>Purification</subject><subject>Recombinant Proteins - isolation & purification</subject><subject>superparamagnetic</subject><subject>Technology, Pharmaceutical - methods</subject><issn>0006-3592</issn><issn>1097-0290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0U1LHDEYB_BQKnVre_ALyEAv9TD65H1yVPFdlOJajyGTyUjsbGabzNhuP71ZVz0IBQ8hJPyePyR_hDYx7GAAslv7YYdwIfgHNMGgZAlEwUc0AQBRUq7IOvqc0n0-ykqIT2idCKB58Qm63mseTLAuFT4Upm198MOi6PydCU3ZjsEOvg-m8_9cUwQT-pkZXPSmS0Xbx6L2-eIuuMHbIrm5iWbJv6C1Ngv39XnfQDdHh9ODk_Li6vj0YO-itJwoXiosBFEtZZwIJwluRFWTprXgLG8qzEFYrBivoWaSAaFW1Iw1Ko9hZi2mdAN9X-XOY_97dGnQM5-s6zoTXD8mjaWsgAhO5TsoxwS4xOQdlEpWAacs029v6H0_xvxdS8WqikvKVFbbK2Vjn1J0rZ5HPzNxoTHoZX8696ef-st26zlxrGeueZUvhWWwuwJ_fOcW_0_S-6fTl8hyNeHT4P6-Tpj4S4v8FK5vL4_12TmjWE5_6h_0EU0lsRE</recordid><startdate>201601</startdate><enddate>201601</enddate><creator>Fields, Conor</creator><creator>Li, Peng</creator><creator>O'Mahony, James J.</creator><creator>Lee, Gil U.</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201601</creationdate><title>Advances in affinity ligand-functionalized nanomaterials for biomagnetic separation</title><author>Fields, Conor ; Li, Peng ; O'Mahony, James J. ; Lee, Gil U.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5295-916629f34526e721d68b2dfc0ec5d81506c1945b0b474023c6b44d991614cc133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Affinity</topic><topic>Antibodies</topic><topic>antibody fragments</topic><topic>bioseparation</topic><topic>Biotechnology</topic><topic>Biotechnology - methods</topic><topic>Chromatography</topic><topic>Economics</topic><topic>Fragments</topic><topic>Ligands</topic><topic>Membrane separation</topic><topic>micro-particles</topic><topic>Molecules</topic><topic>Nanomaterials</topic><topic>nanoparticles</topic><topic>Nanostructures</topic><topic>Peptides</topic><topic>Proteins</topic><topic>Purification</topic><topic>Recombinant Proteins - isolation & purification</topic><topic>superparamagnetic</topic><topic>Technology, Pharmaceutical - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fields, Conor</creatorcontrib><creatorcontrib>Li, Peng</creatorcontrib><creatorcontrib>O'Mahony, James J.</creatorcontrib><creatorcontrib>Lee, Gil U.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biotechnology and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fields, Conor</au><au>Li, Peng</au><au>O'Mahony, James J.</au><au>Lee, Gil U.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Advances in affinity ligand-functionalized nanomaterials for biomagnetic separation</atitle><jtitle>Biotechnology and bioengineering</jtitle><addtitle>Biotechnol. 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The downstream processing of proteins remains the most significant cost in protein production, and is largely attributed to rigorous chromatographic purification protocols, where the stringency of purity for biopharmaceutical products sometimes exceeds 99%. With an ever burgeoning biotechnology market, there is a constant demand for alternative purification methodologies, to ameliorate the dependence on chromatography, while still adhering to regulatory concerns over product purity and safety. In this article, we present an up‐to‐date view of bioseparation, with emphasis on magnetic separation and its potential application in the field. Additionally, we discuss the economic and performance benefits of synthetic ligands, in the form of peptides and miniaturized antibody fragments, compared to full‐length antibodies. We propose that adoption of synthetic affinity ligands coupled with magnetic adsorbents, will play an important role in enabling sustainable bioprocessing in the future. Biotechnol. Bioeng. 2016;113: 11–25. © 2015 Wiley Periodicals, Inc.
Magnetic separation, in combination with adoption of synthetic affinity ligands, offers an alternative approach to protein purification and has the potential to alleviate bottleneck issues associated with industrial‐scale downstream chromatographic purification. This review article focuses on magnetic bioseparation, with emphasis on the economic and performance benefits of peptides and antibody fragments, as alternative, synthetic affinity ligand reagents to full‐length antibodies.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>26032605</pmid><doi>10.1002/bit.25665</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Affinity Antibodies antibody fragments bioseparation Biotechnology Biotechnology - methods Chromatography Economics Fragments Ligands Membrane separation micro-particles Molecules Nanomaterials nanoparticles Nanostructures Peptides Proteins Purification Recombinant Proteins - isolation & purification superparamagnetic Technology, Pharmaceutical - methods |
| title | Advances in affinity ligand-functionalized nanomaterials for biomagnetic separation |
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