Bothropoides pauloensis venom effects on isolated perfused kidney and cultured renal tubular epithelial cells
Snake envenomation (Bothrops genus) is common in tropical countries and acute kidney injury is one of the complications observed in Bothrops snakebite with relevant morbidity and mortality. Here, we showed that Bothropoides pauloensis venom (BpV) decreased cell viability (IC50 of 7.5 μg/mL). Flow cy...
Gespeichert in:
| Veröffentlicht in: | Toxicon (Oxford) 2015-12, Vol.108, p.126-133 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 133 |
|---|---|
| container_issue | |
| container_start_page | 126 |
| container_title | Toxicon (Oxford) |
| container_volume | 108 |
| creator | Marinho, Aline D. Morais, Isabel C.O. Lima, Dânya B. Jorge, Antônio R.C. Jorge, Roberta J.B. Menezes, Ramon R.P.P.B. Mello, Clarissa P. Pereira, Gustavo J.S. Silveira, João A.M. Toyama, Marcos H. Orzáez, Mar Martins, Alice M.C. Monteiro, Helena S.A. |
| description | Snake envenomation (Bothrops genus) is common in tropical countries and acute kidney injury is one of the complications observed in Bothrops snakebite with relevant morbidity and mortality. Here, we showed that Bothropoides pauloensis venom (BpV) decreased cell viability (IC50 of 7.5 μg/mL). Flow cytometry with annexin V and propidium iodide showed that cell death occurred predominantly by apoptosis and late apoptosis, through caspases 3 and 7 activation, mitochondrial membrane potential collapse and ROS overproduction. BpV reduced perfusion pressure, renal vascular resistance, urinary flow, glomerular filtration rate, percentage of sodium, chloride or potassium tubular transportation. These findings demonstrated that BpV cytotoxicity on renal epithelial cells might be responsible for the nephrotoxicity observed in isolated kidney.
•Bothropoides pauloensis venom induced apoptosis and necrosis in MDCK cells.•The venom induced caspases 3 and 7 activation, mitochondrial depolarization and ROS overproduction in MDCK cells.•B. pauloensis venom induced alterations in rat isolated kidney. |
| doi_str_mv | 10.1016/j.toxicon.2015.09.031 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1778015172</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S004101011530091X</els_id><sourcerecordid>1751210132</sourcerecordid><originalsourceid>FETCH-LOGICAL-c431t-d6d7bfc55e2371b9d844f35c8c3d438854d95d23a1c650f7642417212e3967733</originalsourceid><addsrcrecordid>eNqNkU9vFSEUxYmxsc_qR9CwdDMjDDDMrIw2rZo06UbXhAd3Up4MjPxp7LeXl_d0q6tLTn7nXnIOQm8o6Smh4_tDX-IvZ2LoB0JFT-aeMPoM7egk545RQZ6jHSGcdqThl-hlzgdCCJvm8QW6HEbeVEp3aP0Uy0OKW3QWMt509RFCdhk_QogrhmUBUzKOAbscvS5g8QZpqbk9fjgb4AnrYLGpvtTUtARBe1zqvnqdMGyuPIB3TTLgfX6FLhbtM7w-zyv0_fbm2_WX7u7-89frj3ed4YyWzo5W7hcjBAxM0v1sJ84XJsxkmOVsmgS3s7AD09SMgixy5AOncqADsHmUkrEr9O60d0vxZ4Vc1Ory8Qc6QKxZUSmnllrz_Acq6NDCYkdUnFCTYs4JFrUlt-r0pChRx1LUQZ1LUcdSFJlVK6X53p5P1P0K9q_rTwsN-HACoGXy6CCpbBwEA9alFr-y0f3jxG97IqEf</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1751210132</pqid></control><display><type>article</type><title>Bothropoides pauloensis venom effects on isolated perfused kidney and cultured renal tubular epithelial cells</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Marinho, Aline D. ; Morais, Isabel C.O. ; Lima, Dânya B. ; Jorge, Antônio R.C. ; Jorge, Roberta J.B. ; Menezes, Ramon R.P.P.B. ; Mello, Clarissa P. ; Pereira, Gustavo J.S. ; Silveira, João A.M. ; Toyama, Marcos H. ; Orzáez, Mar ; Martins, Alice M.C. ; Monteiro, Helena S.A.</creator><creatorcontrib>Marinho, Aline D. ; Morais, Isabel C.O. ; Lima, Dânya B. ; Jorge, Antônio R.C. ; Jorge, Roberta J.B. ; Menezes, Ramon R.P.P.B. ; Mello, Clarissa P. ; Pereira, Gustavo J.S. ; Silveira, João A.M. ; Toyama, Marcos H. ; Orzáez, Mar ; Martins, Alice M.C. ; Monteiro, Helena S.A.</creatorcontrib><description>Snake envenomation (Bothrops genus) is common in tropical countries and acute kidney injury is one of the complications observed in Bothrops snakebite with relevant morbidity and mortality. Here, we showed that Bothropoides pauloensis venom (BpV) decreased cell viability (IC50 of 7.5 μg/mL). Flow cytometry with annexin V and propidium iodide showed that cell death occurred predominantly by apoptosis and late apoptosis, through caspases 3 and 7 activation, mitochondrial membrane potential collapse and ROS overproduction. BpV reduced perfusion pressure, renal vascular resistance, urinary flow, glomerular filtration rate, percentage of sodium, chloride or potassium tubular transportation. These findings demonstrated that BpV cytotoxicity on renal epithelial cells might be responsible for the nephrotoxicity observed in isolated kidney.
•Bothropoides pauloensis venom induced apoptosis and necrosis in MDCK cells.•The venom induced caspases 3 and 7 activation, mitochondrial depolarization and ROS overproduction in MDCK cells.•B. pauloensis venom induced alterations in rat isolated kidney.</description><identifier>ISSN: 0041-0101</identifier><identifier>EISSN: 1879-3150</identifier><identifier>DOI: 10.1016/j.toxicon.2015.09.031</identifier><identifier>PMID: 26410111</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Apoptosis ; Apoptosis - drug effects ; Bothropoides pauloensis ; Bothrops ; Caspase 3 - metabolism ; Caspase 7 - metabolism ; Cell death ; Cell Survival - drug effects ; Crotalid Venoms - toxicity ; Dogs ; Epithelial Cells - drug effects ; Flow Cytometry ; Glomerular Filtration Rate - drug effects ; In Vitro Techniques ; Iodides ; Kidney - drug effects ; Kidney Tubules - drug effects ; Kidneys ; Madin Darby Canine Kidney Cells ; Male ; Membrane Potential, Mitochondrial - drug effects ; Mortality ; Rats, Wistar ; Reactive Oxygen Species - metabolism ; Renal effects ; Sodium ; Toxicity Tests ; Transportation ; Vascular Resistance - drug effects</subject><ispartof>Toxicon (Oxford), 2015-12, Vol.108, p.126-133</ispartof><rights>2015</rights><rights>Copyright © 2015. Published by Elsevier Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c431t-d6d7bfc55e2371b9d844f35c8c3d438854d95d23a1c650f7642417212e3967733</citedby><cites>FETCH-LOGICAL-c431t-d6d7bfc55e2371b9d844f35c8c3d438854d95d23a1c650f7642417212e3967733</cites><orcidid>0000-0003-4027-0390</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S004101011530091X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26410111$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Marinho, Aline D.</creatorcontrib><creatorcontrib>Morais, Isabel C.O.</creatorcontrib><creatorcontrib>Lima, Dânya B.</creatorcontrib><creatorcontrib>Jorge, Antônio R.C.</creatorcontrib><creatorcontrib>Jorge, Roberta J.B.</creatorcontrib><creatorcontrib>Menezes, Ramon R.P.P.B.</creatorcontrib><creatorcontrib>Mello, Clarissa P.</creatorcontrib><creatorcontrib>Pereira, Gustavo J.S.</creatorcontrib><creatorcontrib>Silveira, João A.M.</creatorcontrib><creatorcontrib>Toyama, Marcos H.</creatorcontrib><creatorcontrib>Orzáez, Mar</creatorcontrib><creatorcontrib>Martins, Alice M.C.</creatorcontrib><creatorcontrib>Monteiro, Helena S.A.</creatorcontrib><title>Bothropoides pauloensis venom effects on isolated perfused kidney and cultured renal tubular epithelial cells</title><title>Toxicon (Oxford)</title><addtitle>Toxicon</addtitle><description>Snake envenomation (Bothrops genus) is common in tropical countries and acute kidney injury is one of the complications observed in Bothrops snakebite with relevant morbidity and mortality. Here, we showed that Bothropoides pauloensis venom (BpV) decreased cell viability (IC50 of 7.5 μg/mL). Flow cytometry with annexin V and propidium iodide showed that cell death occurred predominantly by apoptosis and late apoptosis, through caspases 3 and 7 activation, mitochondrial membrane potential collapse and ROS overproduction. BpV reduced perfusion pressure, renal vascular resistance, urinary flow, glomerular filtration rate, percentage of sodium, chloride or potassium tubular transportation. These findings demonstrated that BpV cytotoxicity on renal epithelial cells might be responsible for the nephrotoxicity observed in isolated kidney.
•Bothropoides pauloensis venom induced apoptosis and necrosis in MDCK cells.•The venom induced caspases 3 and 7 activation, mitochondrial depolarization and ROS overproduction in MDCK cells.•B. pauloensis venom induced alterations in rat isolated kidney.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Bothropoides pauloensis</subject><subject>Bothrops</subject><subject>Caspase 3 - metabolism</subject><subject>Caspase 7 - metabolism</subject><subject>Cell death</subject><subject>Cell Survival - drug effects</subject><subject>Crotalid Venoms - toxicity</subject><subject>Dogs</subject><subject>Epithelial Cells - drug effects</subject><subject>Flow Cytometry</subject><subject>Glomerular Filtration Rate - drug effects</subject><subject>In Vitro Techniques</subject><subject>Iodides</subject><subject>Kidney - drug effects</subject><subject>Kidney Tubules - drug effects</subject><subject>Kidneys</subject><subject>Madin Darby Canine Kidney Cells</subject><subject>Male</subject><subject>Membrane Potential, Mitochondrial - drug effects</subject><subject>Mortality</subject><subject>Rats, Wistar</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Renal effects</subject><subject>Sodium</subject><subject>Toxicity Tests</subject><subject>Transportation</subject><subject>Vascular Resistance - drug effects</subject><issn>0041-0101</issn><issn>1879-3150</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU9vFSEUxYmxsc_qR9CwdDMjDDDMrIw2rZo06UbXhAd3Up4MjPxp7LeXl_d0q6tLTn7nXnIOQm8o6Smh4_tDX-IvZ2LoB0JFT-aeMPoM7egk545RQZ6jHSGcdqThl-hlzgdCCJvm8QW6HEbeVEp3aP0Uy0OKW3QWMt509RFCdhk_QogrhmUBUzKOAbscvS5g8QZpqbk9fjgb4AnrYLGpvtTUtARBe1zqvnqdMGyuPIB3TTLgfX6FLhbtM7w-zyv0_fbm2_WX7u7-89frj3ed4YyWzo5W7hcjBAxM0v1sJ84XJsxkmOVsmgS3s7AD09SMgixy5AOncqADsHmUkrEr9O60d0vxZ4Vc1Ory8Qc6QKxZUSmnllrz_Acq6NDCYkdUnFCTYs4JFrUlt-r0pChRx1LUQZ1LUcdSFJlVK6X53p5P1P0K9q_rTwsN-HACoGXy6CCpbBwEA9alFr-y0f3jxG97IqEf</recordid><startdate>20151215</startdate><enddate>20151215</enddate><creator>Marinho, Aline D.</creator><creator>Morais, Isabel C.O.</creator><creator>Lima, Dânya B.</creator><creator>Jorge, Antônio R.C.</creator><creator>Jorge, Roberta J.B.</creator><creator>Menezes, Ramon R.P.P.B.</creator><creator>Mello, Clarissa P.</creator><creator>Pereira, Gustavo J.S.</creator><creator>Silveira, João A.M.</creator><creator>Toyama, Marcos H.</creator><creator>Orzáez, Mar</creator><creator>Martins, Alice M.C.</creator><creator>Monteiro, Helena S.A.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope><scope>8FD</scope><scope>FR3</scope><scope>KR7</scope><orcidid>https://orcid.org/0000-0003-4027-0390</orcidid></search><sort><creationdate>20151215</creationdate><title>Bothropoides pauloensis venom effects on isolated perfused kidney and cultured renal tubular epithelial cells</title><author>Marinho, Aline D. ; Morais, Isabel C.O. ; Lima, Dânya B. ; Jorge, Antônio R.C. ; Jorge, Roberta J.B. ; Menezes, Ramon R.P.P.B. ; Mello, Clarissa P. ; Pereira, Gustavo J.S. ; Silveira, João A.M. ; Toyama, Marcos H. ; Orzáez, Mar ; Martins, Alice M.C. ; Monteiro, Helena S.A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-d6d7bfc55e2371b9d844f35c8c3d438854d95d23a1c650f7642417212e3967733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Bothropoides pauloensis</topic><topic>Bothrops</topic><topic>Caspase 3 - metabolism</topic><topic>Caspase 7 - metabolism</topic><topic>Cell death</topic><topic>Cell Survival - drug effects</topic><topic>Crotalid Venoms - toxicity</topic><topic>Dogs</topic><topic>Epithelial Cells - drug effects</topic><topic>Flow Cytometry</topic><topic>Glomerular Filtration Rate - drug effects</topic><topic>In Vitro Techniques</topic><topic>Iodides</topic><topic>Kidney - drug effects</topic><topic>Kidney Tubules - drug effects</topic><topic>Kidneys</topic><topic>Madin Darby Canine Kidney Cells</topic><topic>Male</topic><topic>Membrane Potential, Mitochondrial - drug effects</topic><topic>Mortality</topic><topic>Rats, Wistar</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Renal effects</topic><topic>Sodium</topic><topic>Toxicity Tests</topic><topic>Transportation</topic><topic>Vascular Resistance - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Marinho, Aline D.</creatorcontrib><creatorcontrib>Morais, Isabel C.O.</creatorcontrib><creatorcontrib>Lima, Dânya B.</creatorcontrib><creatorcontrib>Jorge, Antônio R.C.</creatorcontrib><creatorcontrib>Jorge, Roberta J.B.</creatorcontrib><creatorcontrib>Menezes, Ramon R.P.P.B.</creatorcontrib><creatorcontrib>Mello, Clarissa P.</creatorcontrib><creatorcontrib>Pereira, Gustavo J.S.</creatorcontrib><creatorcontrib>Silveira, João A.M.</creatorcontrib><creatorcontrib>Toyama, Marcos H.</creatorcontrib><creatorcontrib>Orzáez, Mar</creatorcontrib><creatorcontrib>Martins, Alice M.C.</creatorcontrib><creatorcontrib>Monteiro, Helena S.A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Civil Engineering Abstracts</collection><jtitle>Toxicon (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Marinho, Aline D.</au><au>Morais, Isabel C.O.</au><au>Lima, Dânya B.</au><au>Jorge, Antônio R.C.</au><au>Jorge, Roberta J.B.</au><au>Menezes, Ramon R.P.P.B.</au><au>Mello, Clarissa P.</au><au>Pereira, Gustavo J.S.</au><au>Silveira, João A.M.</au><au>Toyama, Marcos H.</au><au>Orzáez, Mar</au><au>Martins, Alice M.C.</au><au>Monteiro, Helena S.A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bothropoides pauloensis venom effects on isolated perfused kidney and cultured renal tubular epithelial cells</atitle><jtitle>Toxicon (Oxford)</jtitle><addtitle>Toxicon</addtitle><date>2015-12-15</date><risdate>2015</risdate><volume>108</volume><spage>126</spage><epage>133</epage><pages>126-133</pages><issn>0041-0101</issn><eissn>1879-3150</eissn><abstract>Snake envenomation (Bothrops genus) is common in tropical countries and acute kidney injury is one of the complications observed in Bothrops snakebite with relevant morbidity and mortality. Here, we showed that Bothropoides pauloensis venom (BpV) decreased cell viability (IC50 of 7.5 μg/mL). Flow cytometry with annexin V and propidium iodide showed that cell death occurred predominantly by apoptosis and late apoptosis, through caspases 3 and 7 activation, mitochondrial membrane potential collapse and ROS overproduction. BpV reduced perfusion pressure, renal vascular resistance, urinary flow, glomerular filtration rate, percentage of sodium, chloride or potassium tubular transportation. These findings demonstrated that BpV cytotoxicity on renal epithelial cells might be responsible for the nephrotoxicity observed in isolated kidney.
•Bothropoides pauloensis venom induced apoptosis and necrosis in MDCK cells.•The venom induced caspases 3 and 7 activation, mitochondrial depolarization and ROS overproduction in MDCK cells.•B. pauloensis venom induced alterations in rat isolated kidney.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>26410111</pmid><doi>10.1016/j.toxicon.2015.09.031</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-4027-0390</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0041-0101 |
| ispartof | Toxicon (Oxford), 2015-12, Vol.108, p.126-133 |
| issn | 0041-0101 1879-3150 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1778015172 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Apoptosis Apoptosis - drug effects Bothropoides pauloensis Bothrops Caspase 3 - metabolism Caspase 7 - metabolism Cell death Cell Survival - drug effects Crotalid Venoms - toxicity Dogs Epithelial Cells - drug effects Flow Cytometry Glomerular Filtration Rate - drug effects In Vitro Techniques Iodides Kidney - drug effects Kidney Tubules - drug effects Kidneys Madin Darby Canine Kidney Cells Male Membrane Potential, Mitochondrial - drug effects Mortality Rats, Wistar Reactive Oxygen Species - metabolism Renal effects Sodium Toxicity Tests Transportation Vascular Resistance - drug effects |
| title | Bothropoides pauloensis venom effects on isolated perfused kidney and cultured renal tubular epithelial cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-04T12%3A05%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Bothropoides%20pauloensis%20venom%20effects%20on%20isolated%20perfused%20kidney%20and%20cultured%20renal%20tubular%20epithelial%20cells&rft.jtitle=Toxicon%20(Oxford)&rft.au=Marinho,%20Aline%20D.&rft.date=2015-12-15&rft.volume=108&rft.spage=126&rft.epage=133&rft.pages=126-133&rft.issn=0041-0101&rft.eissn=1879-3150&rft_id=info:doi/10.1016/j.toxicon.2015.09.031&rft_dat=%3Cproquest_cross%3E1751210132%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1751210132&rft_id=info:pmid/26410111&rft_els_id=S004101011530091X&rfr_iscdi=true |