The effect of disulfide bond introduction and related Cys/Ser mutations on the stability of a cyclohexanone monooxygenase

•Application of an efficient and easy method for the introduction of additional disulfide bonds without the need of a crystal structure.•CHMO variants created with higher thermal as well as oxidative stability without any loss of catalytic activity.•Additional creation and analysis of single Cys/Ser...

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Veröffentlicht in:	Journal of biotechnology 2015-11, Vol.214, p.199-211
Hauptverfasser:	Schmidt, Sandy, Genz, Maika, Balke, Kathleen, Bornscheuer, Uwe T.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acinetobacter Acinetobacter - enzymology Acinetobacter - genetics Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism Bonding Computational design Cyclohexanone Cyclohexanone monooxygenase Cysteine - chemistry Cysteine - genetics Disulfide bonds Disulfides Disulfides - chemistry Disulfides - metabolism Enzyme Stability Enzymes Escherichia coli Esters Long-term stability Mutation Mutations Oxidation Oxidative stability Oxygenases - chemistry Oxygenases - genetics Oxygenases - metabolism Protein Engineering Recombinant Proteins Serine - chemistry Serine - genetics Stability Thermostability
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