A polymer microfluidic chip for quantitative detection of multiple water- and foodborne pathogens using real-time fluorogenic loop-mediated isothermal amplification

Inexpensive, portable, and easy-to-use devices for rapid detection of microbial pathogens are needed to ensure safety of water and food. In this study, a disposable polymer microfluidic chip for quantitative detection of multiple pathogens using isothermal nucleic acid amplification was developed. T...

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Veröffentlicht in:	Biomedical microdevices 2012-08, Vol.14 (4), p.769-778
Hauptverfasser:	Tourlousse, Dieter M., Ahmad, Farhan, Stedtfeld, Robert D., Seyrig, Gregoire, Tiedje, James M., Hashsham, Syed A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amplification Biological and Medical Physics Biomedical engineering Biomedical Engineering and Bioengineering Biophysics Campylobacter jejuni Chips Engineering Engineering Fluid Dynamics Food contamination & poisoning Food Contamination - analysis Food Microbiology - instrumentation Imaging Lamps Limit of Detection Microfluidic Analytical Techniques - economics Microfluidic Analytical Techniques - instrumentation Microfluidics Microorganisms Nanotechnology Pathogens Polymers Real time Salmonella Semiconductors Shigella Spectrometry, Fluorescence - instrumentation Time Factors Vibrio cholerae Water Microbiology Water Pollutants - isolation & purification Water pollution
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