Three-dimensional culture of mouse pancreatic islet on a liver-derived perfusion-decellularized bioscaffold for potential clinical application

The cutting-edge technology of three-dimensional liver decellularized bioscaffold has a potential to provide a microenvironment that is suitable for the resident cells and even develop a new functional organ. Liver decellularized bioscaffold preserved the native extracellular matrix and three-dimens...

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Veröffentlicht in:Journal of biomaterials applications 2015-10, Vol.30 (4), p.379-387
Hauptverfasser: Xu, Tianxin, Zhu, Mingyan, Guo, Yibing, Wu, Di, Huang, Yan, Fan, Xiangjun, Zhu, Shajun, Lin, Changchun, Li, Xiaohong, Lu, Jingjing, Zhu, Hui, Zhou, Pengcheng, Lu, Yuhua, Wang, Zhiwei
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container_issue 4
container_start_page 379
container_title Journal of biomaterials applications
container_volume 30
creator Xu, Tianxin
Zhu, Mingyan
Guo, Yibing
Wu, Di
Huang, Yan
Fan, Xiangjun
Zhu, Shajun
Lin, Changchun
Li, Xiaohong
Lu, Jingjing
Zhu, Hui
Zhou, Pengcheng
Lu, Yuhua
Wang, Zhiwei
description The cutting-edge technology of three-dimensional liver decellularized bioscaffold has a potential to provide a microenvironment that is suitable for the resident cells and even develop a new functional organ. Liver decellularized bioscaffold preserved the native extracellular matrix and three-dimensional architecture in support of the cell culture. The goal of this study was to discover if three-dimensional extracellular matrix derived from mouse liver could facilitate the growth and maintenance of physiological functions of mouse isolated islets. We generated a whole organ liver decellularized bioscaffold which could successfully preserve extracellular matrix proteins and the native vascular channels using 1% Triton X-100/0.1% ammonium protocol. To evaluate the potential of decellularized liver as a scaffold for islets transplantation, the liver decellularized bioscaffold was infused with mouse primary pancreatic islets which were obtained through Collagenase P digestion protocol. Its yield, morphology, and quality were estimated by microscopic analysis, dithizone staining, insulin immunofluorescence and glucose stimulation experiments. Comparing the three-dimensional culture in liver decellularized bioscaffold with the orthodoxy two-dimensional plate culture, hematoxylin-eosin staining, immunohistochemistry, and insulin gene expression were tested. Our results demonstrated that the liver decellularized bioscaffold could support cellular culture and maintenance of cell functions. In contrast with the conventional two-dimensional culture, three-dimensional culture system could give rise to an up-regulated insulin gene expression. These findings demonstrated that the liver bioscaffold by a perfusion-decellularized technique could serve as a platform to support the survival and function of the pancreatic islets in vitro. Meanwhile three-dimensional culture system had a superior role in contrast with the two-dimensional culture. This study advanced the field of regenerative medicine towards the development of a liver decellularized bioscaffold capable of forming a neo-organ and could be used as potential clinical application.
doi_str_mv 10.1177/0885328215587610
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subjects Animals
Cells, Cultured
Culture
Extracellular Matrix - chemistry
Gene expression
Insulin
Islets of Langerhans - cytology
Islets of Langerhans Transplantation
Liver
Liver - chemistry
Maintenance
Male
Mice
Mice, Inbred C57BL
Primary Cell Culture - methods
Staining
Three dimensional
Tissue Engineering - methods
Tissue Scaffolds - chemistry
Two dimensional
title Three-dimensional culture of mouse pancreatic islet on a liver-derived perfusion-decellularized bioscaffold for potential clinical application
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