Enhanced H2 Production and Redirected Metabolic Flux via Overexpression of fhlA and pncB in Klebsiella HQ-3 Strain

Genetic modifications are considered as one of the most important technologies for improving fermentative hydrogen yield. Herein, we overexpress fhlA and pncB genes from Klebsiella HQ-3 independently to enhance hydrogen molar yield. HQ-3- fhlA/pncB strain is developed by manipulation of pET28-Pkan/...

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Veröffentlicht in:	Applied biochemistry and biotechnology 2016-03, Vol.178 (6), p.1113-1128
Hauptverfasser:	Jawed, Muhammad, Pi, Jian, Xu, Li, Zhang, Houjin, Hakeem, Abdul, Yan, Yunjun
Format:	Artikel
Sprache:	eng
Schlagworte:	Anaerobiosis Bacteria Bacterial Proteins - genetics Bacterial Proteins - metabolism Biochemistry Biohydrogen Biotechnology Chemistry Chemistry and Materials Science Electrophoresis, Polyacrylamide Gel Enzymes Ethanol Fermentation Gene expression Hydrogen - metabolism Hydrogen-Ion Concentration Klebsiella - genetics Klebsiella - metabolism Metabolites Temperature
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