Metal-Dependent DNA Cleavage Mechanism of the I-CreI LAGLIDADG Homing Endonuclease

The LAGLIDADG homing endonucleases include free-standing homodimers, pseudosymmetric monomers, and related enzyme domains embedded within inteins. DNA-bound structures of homodimeric I-CreI and monomeric I-SceI indicate that three catalytic divalent metal ions are distributed across a pair of overla...

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Veröffentlicht in:	Biochemistry (Easton) 2004-11, Vol.43 (44), p.14015-14026
Hauptverfasser:	Chevalier, Brett, Sussman, Django, Otis, Christian, Noël, Ann-Josée, Turmel, Monique, Lemieux, Claude, Stephens, Kathy, Monnat, Raymond J, Stoddard, Barry L
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Motifs - genetics Animals Binding Sites - genetics Catalysis Cations, Divalent - chemistry Chlamydomonas reinhardtii - enzymology Chlamydomonas reinhardtii - genetics Crystallography, X-Ray DNA Restriction Enzymes - chemistry DNA Restriction Enzymes - genetics DNA Restriction Enzymes - metabolism DNA, Algal - chemistry DNA, Algal - metabolism DNA, Chloroplast - chemistry DNA, Chloroplast - metabolism DNA-Binding Proteins - chemistry DNA-Binding Proteins - metabolism Hydrolysis Kinetics Lysine - genetics Lysine - metabolism Metals - chemistry Point Mutation Protein Binding - genetics Substrate Specificity - genetics
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