Epigallocatechin gallate attenuates cardiopulmonary bypass–associated lung injury
Abstract Background Lung dysfunction constitutes a severe complication after major cardiac surgery with cardiopulmonary bypass (CPB), substantially contributing to postoperative morbidity and mortality. The current possibilities of preventive and therapeutic interventions, however, remain insufficie...
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| Veröffentlicht in: | The Journal of surgical research 2016-04, Vol.201 (2), p.313-325 |
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| creator | Kasper, Bernhard Salameh, Aida, MD, PhD Krausch, Miriam, DVM Kiefer, Philipp, MD Kostelka, Martin, MD Mohr, Friedrich Wilhelm, MD, PhD Dhein, Stefan, MD, PhD |
| description | Abstract Background Lung dysfunction constitutes a severe complication after major cardiac surgery with cardiopulmonary bypass (CPB), substantially contributing to postoperative morbidity and mortality. The current possibilities of preventive and therapeutic interventions, however, remain insufficient. We, therefore, investigated the effects of intraoperative application of the antioxidant and anti-inflammatory green tea polyphenol (–)–epigallocatechin-3-gallate (EGCG) on CPB-associated lung injury. Materials and methods Thirty piglets (8–15 kg) were divided into four groups: sham-operated and saline-treated control group ( n = 7); sham-operated and EGCG-treated control group (EGCG-control group; n = 7); CPB group ( n = 10); and CPB + EGCG group ( n = 6). The CPB groups underwent 120 min of CPB followed by 90 min of recovery time. In the CPB + EGCG group, EGCG (10 mg/kg body weight) was administered intravenously before and after CPB. Hemodynamic monitoring, blood gas analysis, hematoxylin-eosin staining, and immunohistochemistry of lung tissue were performed. Results Histologic examination revealed thickening of the alveolar wall and enhanced alveolar neutrophil infiltration in the CPB group ( P |
| doi_str_mv | 10.1016/j.jss.2015.11.007 |
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The current possibilities of preventive and therapeutic interventions, however, remain insufficient. We, therefore, investigated the effects of intraoperative application of the antioxidant and anti-inflammatory green tea polyphenol (–)–epigallocatechin-3-gallate (EGCG) on CPB-associated lung injury. Materials and methods Thirty piglets (8–15 kg) were divided into four groups: sham-operated and saline-treated control group ( n = 7); sham-operated and EGCG-treated control group (EGCG-control group; n = 7); CPB group ( n = 10); and CPB + EGCG group ( n = 6). The CPB groups underwent 120 min of CPB followed by 90 min of recovery time. In the CPB + EGCG group, EGCG (10 mg/kg body weight) was administered intravenously before and after CPB. Hemodynamic monitoring, blood gas analysis, hematoxylin-eosin staining, and immunohistochemistry of lung tissue were performed. Results Histologic examination revealed thickening of the alveolar wall and enhanced alveolar neutrophil infiltration in the CPB group ( P < 0.05) compared with those in the control group, which was prevented by EGCG ( P < 0.05). In the CPB group, higher formation of poly(ADP-ribose) and nuclear translocation of apoptosis-inducing factor was detected in comparison with those in the control group ( P < 0.001), which were both reduced in the CPB + EGCG group ( P < 0.001). Compared with the control group, the EGCG-control group showed thickening of the alveolar wall and increased neutrophil infiltration ( P < 0.05). Conclusions CPB leads to lung edema, pulmonary neutrophil infiltration, and presumably initiation of poly(ADP-ribose) polymerase–dependent cell death signaling in the lung. EGCG appears to attenuate CPB-associated lung injury, suggesting that this may provide a novel pharmacologic approach.</description><identifier>ISSN: 0022-4804</identifier><identifier>EISSN: 1095-8673</identifier><identifier>DOI: 10.1016/j.jss.2015.11.007</identifier><identifier>PMID: 27020813</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Antioxidants - therapeutic use ; Apoptosis Inducing Factor - analysis ; Apoptosis-inducing factor ; Camellia sinensis ; Cardiopulmonary bypass ; Cardiopulmonary Bypass - adverse effects ; Catechin - analogs & derivatives ; Catechin - therapeutic use ; Drug Evaluation, Preclinical ; Epigallocatechin gallate ; Female ; Immunohistochemistry ; Lung - chemistry ; Lung - pathology ; Lung injury ; Lung Injury - etiology ; Lung Injury - pathology ; Lung Injury - prevention & control ; Male ; Phytotherapy ; Plant Extracts - therapeutic use ; Poly Adenosine Diphosphate Ribose - analysis ; Poly(ADP-ribose) polymerase ; Surgery ; Swine ; Tumor Necrosis Factor-alpha - analysis ; Tyrosine - analogs & derivatives ; Tyrosine - analysis</subject><ispartof>The Journal of surgical research, 2016-04, Vol.201 (2), p.313-325</ispartof><rights>Elsevier Inc.</rights><rights>2016 Elsevier Inc.</rights><rights>Copyright © 2016 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-5a2c421861f4ea01d01101fad72870f98413fdd5b62b055aed51d3eb0c240c003</citedby><cites>FETCH-LOGICAL-c408t-5a2c421861f4ea01d01101fad72870f98413fdd5b62b055aed51d3eb0c240c003</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0022480415011233$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27020813$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kasper, Bernhard</creatorcontrib><creatorcontrib>Salameh, Aida, MD, PhD</creatorcontrib><creatorcontrib>Krausch, Miriam, DVM</creatorcontrib><creatorcontrib>Kiefer, Philipp, MD</creatorcontrib><creatorcontrib>Kostelka, Martin, MD</creatorcontrib><creatorcontrib>Mohr, Friedrich Wilhelm, MD, PhD</creatorcontrib><creatorcontrib>Dhein, Stefan, MD, PhD</creatorcontrib><title>Epigallocatechin gallate attenuates cardiopulmonary bypass–associated lung injury</title><title>The Journal of surgical research</title><addtitle>J Surg Res</addtitle><description>Abstract Background Lung dysfunction constitutes a severe complication after major cardiac surgery with cardiopulmonary bypass (CPB), substantially contributing to postoperative morbidity and mortality. The current possibilities of preventive and therapeutic interventions, however, remain insufficient. We, therefore, investigated the effects of intraoperative application of the antioxidant and anti-inflammatory green tea polyphenol (–)–epigallocatechin-3-gallate (EGCG) on CPB-associated lung injury. Materials and methods Thirty piglets (8–15 kg) were divided into four groups: sham-operated and saline-treated control group ( n = 7); sham-operated and EGCG-treated control group (EGCG-control group; n = 7); CPB group ( n = 10); and CPB + EGCG group ( n = 6). The CPB groups underwent 120 min of CPB followed by 90 min of recovery time. In the CPB + EGCG group, EGCG (10 mg/kg body weight) was administered intravenously before and after CPB. Hemodynamic monitoring, blood gas analysis, hematoxylin-eosin staining, and immunohistochemistry of lung tissue were performed. Results Histologic examination revealed thickening of the alveolar wall and enhanced alveolar neutrophil infiltration in the CPB group ( P < 0.05) compared with those in the control group, which was prevented by EGCG ( P < 0.05). In the CPB group, higher formation of poly(ADP-ribose) and nuclear translocation of apoptosis-inducing factor was detected in comparison with those in the control group ( P < 0.001), which were both reduced in the CPB + EGCG group ( P < 0.001). Compared with the control group, the EGCG-control group showed thickening of the alveolar wall and increased neutrophil infiltration ( P < 0.05). Conclusions CPB leads to lung edema, pulmonary neutrophil infiltration, and presumably initiation of poly(ADP-ribose) polymerase–dependent cell death signaling in the lung. EGCG appears to attenuate CPB-associated lung injury, suggesting that this may provide a novel pharmacologic approach.</description><subject>Animals</subject><subject>Antioxidants - therapeutic use</subject><subject>Apoptosis Inducing Factor - analysis</subject><subject>Apoptosis-inducing factor</subject><subject>Camellia sinensis</subject><subject>Cardiopulmonary bypass</subject><subject>Cardiopulmonary Bypass - adverse effects</subject><subject>Catechin - analogs & derivatives</subject><subject>Catechin - therapeutic use</subject><subject>Drug Evaluation, Preclinical</subject><subject>Epigallocatechin gallate</subject><subject>Female</subject><subject>Immunohistochemistry</subject><subject>Lung - chemistry</subject><subject>Lung - pathology</subject><subject>Lung injury</subject><subject>Lung Injury - etiology</subject><subject>Lung Injury - pathology</subject><subject>Lung Injury - prevention & control</subject><subject>Male</subject><subject>Phytotherapy</subject><subject>Plant Extracts - therapeutic use</subject><subject>Poly Adenosine Diphosphate Ribose - analysis</subject><subject>Poly(ADP-ribose) polymerase</subject><subject>Surgery</subject><subject>Swine</subject><subject>Tumor Necrosis Factor-alpha - analysis</subject><subject>Tyrosine - analogs & derivatives</subject><subject>Tyrosine - analysis</subject><issn>0022-4804</issn><issn>1095-8673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFu1DAQhi0EotvCA3BBOXJJmLGTOCskJFQVilSJQ-FsOfakOGTtYCeV9sY78IY8CY62cODAxZ6R_n80_zeMvUCoELB9PVZjShUHbCrECkA-YjuEfVN2rRSP2Q6A87LuoD5j5ymNkPu9FE_ZGZfAoUOxY7dXs7vT0xSMXsh8db7YulwXelnIr7lKhdHRujCv0yF4HY9Ff5x1Sr9-_MxvMC5rbDGt_q5wflzj8Rl7Mugp0fOH_4J9eX_1-fK6vPn04ePlu5vS1NAtZaO5qTl2LQ41aUALmFMN2kreSRj2XY1isLbpW95D02iyDVpBPRhegwEQF-zVae4cw_eV0qIOLhnK63sKa1IopQQp232XpXiSmhhSijSoObpDzqIQ1MZSjSqzVBtLhagyy-x5-TB-7Q9k_zr-wMuCNycB5ZD3jqJKxpE3ZF0ksygb3H_Hv_3HbSbnndHTNzpSGsMafaanUCWuQN1ux9xuiU3mxIUQvwF_1Ztj</recordid><startdate>20160401</startdate><enddate>20160401</enddate><creator>Kasper, Bernhard</creator><creator>Salameh, Aida, MD, PhD</creator><creator>Krausch, Miriam, DVM</creator><creator>Kiefer, Philipp, MD</creator><creator>Kostelka, Martin, MD</creator><creator>Mohr, Friedrich Wilhelm, MD, PhD</creator><creator>Dhein, Stefan, MD, PhD</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20160401</creationdate><title>Epigallocatechin gallate attenuates cardiopulmonary bypass–associated lung injury</title><author>Kasper, Bernhard ; Salameh, Aida, MD, PhD ; Krausch, Miriam, DVM ; Kiefer, Philipp, MD ; Kostelka, Martin, MD ; Mohr, Friedrich Wilhelm, MD, PhD ; Dhein, Stefan, MD, PhD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-5a2c421861f4ea01d01101fad72870f98413fdd5b62b055aed51d3eb0c240c003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Antioxidants - therapeutic use</topic><topic>Apoptosis Inducing Factor - analysis</topic><topic>Apoptosis-inducing factor</topic><topic>Camellia sinensis</topic><topic>Cardiopulmonary bypass</topic><topic>Cardiopulmonary Bypass - adverse effects</topic><topic>Catechin - analogs & derivatives</topic><topic>Catechin - therapeutic use</topic><topic>Drug Evaluation, Preclinical</topic><topic>Epigallocatechin gallate</topic><topic>Female</topic><topic>Immunohistochemistry</topic><topic>Lung - chemistry</topic><topic>Lung - pathology</topic><topic>Lung injury</topic><topic>Lung Injury - etiology</topic><topic>Lung Injury - pathology</topic><topic>Lung Injury - prevention & control</topic><topic>Male</topic><topic>Phytotherapy</topic><topic>Plant Extracts - therapeutic use</topic><topic>Poly Adenosine Diphosphate Ribose - analysis</topic><topic>Poly(ADP-ribose) polymerase</topic><topic>Surgery</topic><topic>Swine</topic><topic>Tumor Necrosis Factor-alpha - analysis</topic><topic>Tyrosine - analogs & derivatives</topic><topic>Tyrosine - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kasper, Bernhard</creatorcontrib><creatorcontrib>Salameh, Aida, MD, PhD</creatorcontrib><creatorcontrib>Krausch, Miriam, DVM</creatorcontrib><creatorcontrib>Kiefer, Philipp, MD</creatorcontrib><creatorcontrib>Kostelka, Martin, MD</creatorcontrib><creatorcontrib>Mohr, Friedrich Wilhelm, MD, PhD</creatorcontrib><creatorcontrib>Dhein, Stefan, MD, PhD</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of surgical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kasper, Bernhard</au><au>Salameh, Aida, MD, PhD</au><au>Krausch, Miriam, DVM</au><au>Kiefer, Philipp, MD</au><au>Kostelka, Martin, MD</au><au>Mohr, Friedrich Wilhelm, MD, PhD</au><au>Dhein, Stefan, MD, PhD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Epigallocatechin gallate attenuates cardiopulmonary bypass–associated lung injury</atitle><jtitle>The Journal of surgical research</jtitle><addtitle>J Surg Res</addtitle><date>2016-04-01</date><risdate>2016</risdate><volume>201</volume><issue>2</issue><spage>313</spage><epage>325</epage><pages>313-325</pages><issn>0022-4804</issn><eissn>1095-8673</eissn><abstract>Abstract Background Lung dysfunction constitutes a severe complication after major cardiac surgery with cardiopulmonary bypass (CPB), substantially contributing to postoperative morbidity and mortality. The current possibilities of preventive and therapeutic interventions, however, remain insufficient. We, therefore, investigated the effects of intraoperative application of the antioxidant and anti-inflammatory green tea polyphenol (–)–epigallocatechin-3-gallate (EGCG) on CPB-associated lung injury. Materials and methods Thirty piglets (8–15 kg) were divided into four groups: sham-operated and saline-treated control group ( n = 7); sham-operated and EGCG-treated control group (EGCG-control group; n = 7); CPB group ( n = 10); and CPB + EGCG group ( n = 6). The CPB groups underwent 120 min of CPB followed by 90 min of recovery time. In the CPB + EGCG group, EGCG (10 mg/kg body weight) was administered intravenously before and after CPB. Hemodynamic monitoring, blood gas analysis, hematoxylin-eosin staining, and immunohistochemistry of lung tissue were performed. Results Histologic examination revealed thickening of the alveolar wall and enhanced alveolar neutrophil infiltration in the CPB group ( P < 0.05) compared with those in the control group, which was prevented by EGCG ( P < 0.05). In the CPB group, higher formation of poly(ADP-ribose) and nuclear translocation of apoptosis-inducing factor was detected in comparison with those in the control group ( P < 0.001), which were both reduced in the CPB + EGCG group ( P < 0.001). Compared with the control group, the EGCG-control group showed thickening of the alveolar wall and increased neutrophil infiltration ( P < 0.05). Conclusions CPB leads to lung edema, pulmonary neutrophil infiltration, and presumably initiation of poly(ADP-ribose) polymerase–dependent cell death signaling in the lung. EGCG appears to attenuate CPB-associated lung injury, suggesting that this may provide a novel pharmacologic approach.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>27020813</pmid><doi>10.1016/j.jss.2015.11.007</doi><tpages>13</tpages></addata></record> |
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| subjects | Animals Antioxidants - therapeutic use Apoptosis Inducing Factor - analysis Apoptosis-inducing factor Camellia sinensis Cardiopulmonary bypass Cardiopulmonary Bypass - adverse effects Catechin - analogs & derivatives Catechin - therapeutic use Drug Evaluation, Preclinical Epigallocatechin gallate Female Immunohistochemistry Lung - chemistry Lung - pathology Lung injury Lung Injury - etiology Lung Injury - pathology Lung Injury - prevention & control Male Phytotherapy Plant Extracts - therapeutic use Poly Adenosine Diphosphate Ribose - analysis Poly(ADP-ribose) polymerase Surgery Swine Tumor Necrosis Factor-alpha - analysis Tyrosine - analogs & derivatives Tyrosine - analysis |
| title | Epigallocatechin gallate attenuates cardiopulmonary bypass–associated lung injury |
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