Platelet Adhesion and Thrombus Formation in Whole Blood at Arterial Shear Rate at the End of Pregnancy
Problem Platelet reactivity has not been evaluated in integrated functional testing during normal pregnancy. Here, we analysed platelet functions under arterial shear rate in comparison with static conditions. Method of study Thirty pregnant women with uncomplicated pregnancies and 30 healthy non‐pr...
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| Veröffentlicht in: | American journal of reproductive immunology (1989) 2015-12, Vol.74 (6), p.533-541 |
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| container_issue | 6 |
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| container_title | American journal of reproductive immunology (1989) |
| container_volume | 74 |
| creator | Valéra, Marie-Cécile Parant, Olivier Cenac, Claire Arnaud, Catherine Gallini, Adeline Hamdi, Safouane Sié, Pierre Arnal, Jean-François Payrastre, Bernard |
| description | Problem
Platelet reactivity has not been evaluated in integrated functional testing during normal pregnancy. Here, we analysed platelet functions under arterial shear rate in comparison with static conditions.
Method of study
Thirty pregnant women with uncomplicated pregnancies and 30 healthy non‐pregnant women were enrolled in this study. Platelet adhesion to collagen and fibrinogen and subsequent thrombus formation were measured at arterial shear rate in whole blood using a microfluidic and imaging system. Standard light transmission aggregometry, flow cytometry of activation markers in washed platelets and impedance aggregometry in whole blood were also used to assess platelet responsiveness in static conditions.
Results
Compared to non‐pregnant controls, thrombus formation on collagen fibres and firm platelet adhesion on fibrinogen under arterial shear rate were significantly reduced in pregnant women. Platelet aggregometry assays in suspension showed a slight increase in platelet reactivity in pregnant women.
Conclusion
While platelet aggregometry and platelet activation markers in static conditions show little changes in platelet reactivity, monitoring of platelet adhesion and thrombus growth on collagen or fibrinogen under flow condition in whole blood indicates a significant decrease in pregnant women compared to controls. This decrease might contribute to counteract a hypercoagulable state and to reduce the risk of arterial thrombosis. |
| doi_str_mv | 10.1111/aji.12433 |
| format | Article |
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Platelet reactivity has not been evaluated in integrated functional testing during normal pregnancy. Here, we analysed platelet functions under arterial shear rate in comparison with static conditions.
Method of study
Thirty pregnant women with uncomplicated pregnancies and 30 healthy non‐pregnant women were enrolled in this study. Platelet adhesion to collagen and fibrinogen and subsequent thrombus formation were measured at arterial shear rate in whole blood using a microfluidic and imaging system. Standard light transmission aggregometry, flow cytometry of activation markers in washed platelets and impedance aggregometry in whole blood were also used to assess platelet responsiveness in static conditions.
Results
Compared to non‐pregnant controls, thrombus formation on collagen fibres and firm platelet adhesion on fibrinogen under arterial shear rate were significantly reduced in pregnant women. Platelet aggregometry assays in suspension showed a slight increase in platelet reactivity in pregnant women.
Conclusion
While platelet aggregometry and platelet activation markers in static conditions show little changes in platelet reactivity, monitoring of platelet adhesion and thrombus growth on collagen or fibrinogen under flow condition in whole blood indicates a significant decrease in pregnant women compared to controls. This decrease might contribute to counteract a hypercoagulable state and to reduce the risk of arterial thrombosis.</description><identifier>ISSN: 1046-7408</identifier><identifier>EISSN: 1600-0897</identifier><identifier>DOI: 10.1111/aji.12433</identifier><identifier>PMID: 26435170</identifier><language>eng</language><publisher>Denmark: Blackwell Publishing Ltd</publisher><subject>Adhesion ; Adult ; Arteries - pathology ; Blood clots ; Blood Coagulation ; Blood platelets ; Blood Platelets - physiology ; Cell Adhesion ; Collagen ; Collagen - metabolism ; Female ; Fibrinogen - metabolism ; Humans ; Microfluidics ; Platelet Activation ; platelets ; pregnancy ; Pregnancy - physiology ; Pregnancy Trimesters ; shear rate ; thrombus growth ; Whole Blood Coagulation Time</subject><ispartof>American journal of reproductive immunology (1989), 2015-12, Vol.74 (6), p.533-541</ispartof><rights>2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><rights>2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5643-8f575313572f800f3563999aecf38ccb7b1cf6241e74ad372cfa4c9a2f7a678c3</citedby><cites>FETCH-LOGICAL-c5643-8f575313572f800f3563999aecf38ccb7b1cf6241e74ad372cfa4c9a2f7a678c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Faji.12433$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Faji.12433$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26435170$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Valéra, Marie-Cécile</creatorcontrib><creatorcontrib>Parant, Olivier</creatorcontrib><creatorcontrib>Cenac, Claire</creatorcontrib><creatorcontrib>Arnaud, Catherine</creatorcontrib><creatorcontrib>Gallini, Adeline</creatorcontrib><creatorcontrib>Hamdi, Safouane</creatorcontrib><creatorcontrib>Sié, Pierre</creatorcontrib><creatorcontrib>Arnal, Jean-François</creatorcontrib><creatorcontrib>Payrastre, Bernard</creatorcontrib><title>Platelet Adhesion and Thrombus Formation in Whole Blood at Arterial Shear Rate at the End of Pregnancy</title><title>American journal of reproductive immunology (1989)</title><addtitle>Am J Reprod Immunol</addtitle><description>Problem
Platelet reactivity has not been evaluated in integrated functional testing during normal pregnancy. Here, we analysed platelet functions under arterial shear rate in comparison with static conditions.
Method of study
Thirty pregnant women with uncomplicated pregnancies and 30 healthy non‐pregnant women were enrolled in this study. Platelet adhesion to collagen and fibrinogen and subsequent thrombus formation were measured at arterial shear rate in whole blood using a microfluidic and imaging system. Standard light transmission aggregometry, flow cytometry of activation markers in washed platelets and impedance aggregometry in whole blood were also used to assess platelet responsiveness in static conditions.
Results
Compared to non‐pregnant controls, thrombus formation on collagen fibres and firm platelet adhesion on fibrinogen under arterial shear rate were significantly reduced in pregnant women. Platelet aggregometry assays in suspension showed a slight increase in platelet reactivity in pregnant women.
Conclusion
While platelet aggregometry and platelet activation markers in static conditions show little changes in platelet reactivity, monitoring of platelet adhesion and thrombus growth on collagen or fibrinogen under flow condition in whole blood indicates a significant decrease in pregnant women compared to controls. This decrease might contribute to counteract a hypercoagulable state and to reduce the risk of arterial thrombosis.</description><subject>Adhesion</subject><subject>Adult</subject><subject>Arteries - pathology</subject><subject>Blood clots</subject><subject>Blood Coagulation</subject><subject>Blood platelets</subject><subject>Blood Platelets - physiology</subject><subject>Cell Adhesion</subject><subject>Collagen</subject><subject>Collagen - metabolism</subject><subject>Female</subject><subject>Fibrinogen - metabolism</subject><subject>Humans</subject><subject>Microfluidics</subject><subject>Platelet Activation</subject><subject>platelets</subject><subject>pregnancy</subject><subject>Pregnancy - physiology</subject><subject>Pregnancy Trimesters</subject><subject>shear rate</subject><subject>thrombus growth</subject><subject>Whole Blood Coagulation Time</subject><issn>1046-7408</issn><issn>1600-0897</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUhS0EomVgwQsgS2zKIq0d_yXLadVpiyoYYKBLy-NckwxOXOxEdN4eD9N2gYSEN7aOvnN0rw9Cryk5pvmcmE13TEvO2BN0SCUhBalq9TS_CZeF4qQ6QC9S2hCSdaaeo4NSciaoIofILb0ZwcOI500LqQsDNkODV20M_XpKeBFib8ad3A34pg0e8KkPocEmO-IIsTMef2nBRPw5B-3ksQV8njOCw8sI3wcz2O1L9MwZn-DV_T1DXxfnq7PL4vrjxdXZ_LqwIo9UVE4owSgTqnQVIY4Jyeq6NmAdq6xdqzW1TpacguKmYaq0znBbm9IpI1Vl2Qwd7XNvY_g5QRp13yUL3psBwpQ0VUpKWQtW_Qcq83cJngeaobd_oZswxSEvkimhSCU5p5l6t6dsDClFcPo2dr2JW02J3vWkc0_6T0-ZfXOfOK17aB7Jh2IycLIHfnUetv9O0vP3Vw-Rxd7RpRHuHh0m_tBSMSX0zYcLXX5alYtvy1NN2W_onqjt</recordid><startdate>201512</startdate><enddate>201512</enddate><creator>Valéra, Marie-Cécile</creator><creator>Parant, Olivier</creator><creator>Cenac, Claire</creator><creator>Arnaud, Catherine</creator><creator>Gallini, Adeline</creator><creator>Hamdi, Safouane</creator><creator>Sié, Pierre</creator><creator>Arnal, Jean-François</creator><creator>Payrastre, Bernard</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>201512</creationdate><title>Platelet Adhesion and Thrombus Formation in Whole Blood at Arterial Shear Rate at the End of Pregnancy</title><author>Valéra, Marie-Cécile ; Parant, Olivier ; Cenac, Claire ; Arnaud, Catherine ; Gallini, Adeline ; Hamdi, Safouane ; Sié, Pierre ; Arnal, Jean-François ; Payrastre, Bernard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5643-8f575313572f800f3563999aecf38ccb7b1cf6241e74ad372cfa4c9a2f7a678c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adhesion</topic><topic>Adult</topic><topic>Arteries - pathology</topic><topic>Blood clots</topic><topic>Blood Coagulation</topic><topic>Blood platelets</topic><topic>Blood Platelets - physiology</topic><topic>Cell Adhesion</topic><topic>Collagen</topic><topic>Collagen - metabolism</topic><topic>Female</topic><topic>Fibrinogen - metabolism</topic><topic>Humans</topic><topic>Microfluidics</topic><topic>Platelet Activation</topic><topic>platelets</topic><topic>pregnancy</topic><topic>Pregnancy - physiology</topic><topic>Pregnancy Trimesters</topic><topic>shear rate</topic><topic>thrombus growth</topic><topic>Whole Blood Coagulation Time</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Valéra, Marie-Cécile</creatorcontrib><creatorcontrib>Parant, Olivier</creatorcontrib><creatorcontrib>Cenac, Claire</creatorcontrib><creatorcontrib>Arnaud, Catherine</creatorcontrib><creatorcontrib>Gallini, Adeline</creatorcontrib><creatorcontrib>Hamdi, Safouane</creatorcontrib><creatorcontrib>Sié, Pierre</creatorcontrib><creatorcontrib>Arnal, Jean-François</creatorcontrib><creatorcontrib>Payrastre, Bernard</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of reproductive immunology (1989)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Valéra, Marie-Cécile</au><au>Parant, Olivier</au><au>Cenac, Claire</au><au>Arnaud, Catherine</au><au>Gallini, Adeline</au><au>Hamdi, Safouane</au><au>Sié, Pierre</au><au>Arnal, Jean-François</au><au>Payrastre, Bernard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Platelet Adhesion and Thrombus Formation in Whole Blood at Arterial Shear Rate at the End of Pregnancy</atitle><jtitle>American journal of reproductive immunology (1989)</jtitle><addtitle>Am J Reprod Immunol</addtitle><date>2015-12</date><risdate>2015</risdate><volume>74</volume><issue>6</issue><spage>533</spage><epage>541</epage><pages>533-541</pages><issn>1046-7408</issn><eissn>1600-0897</eissn><abstract>Problem
Platelet reactivity has not been evaluated in integrated functional testing during normal pregnancy. Here, we analysed platelet functions under arterial shear rate in comparison with static conditions.
Method of study
Thirty pregnant women with uncomplicated pregnancies and 30 healthy non‐pregnant women were enrolled in this study. Platelet adhesion to collagen and fibrinogen and subsequent thrombus formation were measured at arterial shear rate in whole blood using a microfluidic and imaging system. Standard light transmission aggregometry, flow cytometry of activation markers in washed platelets and impedance aggregometry in whole blood were also used to assess platelet responsiveness in static conditions.
Results
Compared to non‐pregnant controls, thrombus formation on collagen fibres and firm platelet adhesion on fibrinogen under arterial shear rate were significantly reduced in pregnant women. Platelet aggregometry assays in suspension showed a slight increase in platelet reactivity in pregnant women.
Conclusion
While platelet aggregometry and platelet activation markers in static conditions show little changes in platelet reactivity, monitoring of platelet adhesion and thrombus growth on collagen or fibrinogen under flow condition in whole blood indicates a significant decrease in pregnant women compared to controls. This decrease might contribute to counteract a hypercoagulable state and to reduce the risk of arterial thrombosis.</abstract><cop>Denmark</cop><pub>Blackwell Publishing Ltd</pub><pmid>26435170</pmid><doi>10.1111/aji.12433</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1046-7408 |
| ispartof | American journal of reproductive immunology (1989), 2015-12, Vol.74 (6), p.533-541 |
| issn | 1046-7408 1600-0897 |
| language | eng |
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| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Adhesion Adult Arteries - pathology Blood clots Blood Coagulation Blood platelets Blood Platelets - physiology Cell Adhesion Collagen Collagen - metabolism Female Fibrinogen - metabolism Humans Microfluidics Platelet Activation platelets pregnancy Pregnancy - physiology Pregnancy Trimesters shear rate thrombus growth Whole Blood Coagulation Time |
| title | Platelet Adhesion and Thrombus Formation in Whole Blood at Arterial Shear Rate at the End of Pregnancy |
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