Characterisation of the genes involved in the biosynthesis and attachment of the aminodeoxysugar D-forosamine in the auricin gene cluster of Streptomyces aureofaciens CCM3239
We previously identified the aur1 gene cluster which produces the angucycline antibiotic auricin. Preliminary characterisation of auricin revealed that it is modified by a single aminodeoxysugar, D-forosamine. Here we characterise the D-forosamine-specific genes. The four close tandem genes, aur1TQS...
Gespeichert in:
| Veröffentlicht in: | Applied microbiology and biotechnology 2016-04, Vol.100 (7), p.3177-3195 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 3195 |
|---|---|
| container_issue | 7 |
| container_start_page | 3177 |
| container_title | Applied microbiology and biotechnology |
| container_volume | 100 |
| creator | Bekeova, Carmen Rehakova, Alena Feckova, Lubomira Vlckova, Silvia Novakova, Renata Mingyar, Erik Kormanec, Jan |
| description | We previously identified the aur1 gene cluster which produces the angucycline antibiotic auricin. Preliminary characterisation of auricin revealed that it is modified by a single aminodeoxysugar, D-forosamine. Here we characterise the D-forosamine-specific genes. The four close tandem genes, aur1TQSV, encoding enzymes involved in the initial steps of the deoxysugar biosynthesis, were located on a large operon with other core auricin biosynthetic genes. Deleting these genes resulted in the absence of auricin and the production of deglycosylated auricin intermediates. The two final D-forosamine biosynthetic genes, sa59, an NDP-hexose aminotransferase, and sa52, an NDP-aminohexose N-dimethyltransferase, are located in a region rather distant from the core auricin genes. A deletion analysis of these genes confirmed their role in D-forosamine biosynthesis. The Δsa59 mutant had a phenotype similar to that of the cluster deletion mutant, while the Δsa52 mutant produced an auricin with a demethylated D-forosamine. Although auricin contains a single deoxyhexose, two glycosyltransferase genes were found to participate in the attachment of D-forosamine to the auricin aglycon. An analysis of the expression of the D-forosamine biosynthesis genes revealed that the initial D-forosamine biosynthetic genes aur1TQSV are regulated together with the other auricin core genes by the aur1Ap promoter under the control of the auricin-specific activator Aur1P. The expression of the other D-forosamine genes, however, is governed by promoters differentially dependent upon the two SARP family auricin-specific activators Aur1PR3 and Aur1PR4. These promoters contain direct repeats similar to the SARP consensus sequence and are involved in the interaction with both regulators. |
| doi_str_mv | 10.1007/s00253-015-7214-9 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_1776656887</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A445911129</galeid><sourcerecordid>A445911129</sourcerecordid><originalsourceid>FETCH-LOGICAL-c637t-135d5a0ae15b71e9400d17c676d390e36cc966394a36f964c134c90f902b70413</originalsourceid><addsrcrecordid>eNqNkk1v1DAQhiMEoqXwA7hApF7gkOKP2I6P1fJVqQiJpWfL60yyrhJ7sZ2q-6f4jTjdLrAIIeSDRzPPO6PRvEXxHKMzjJB4ExEijFYIs0oQXFfyQXGMa0oqxHH9sDhGWOQKk81R8STGa4QwaTh_XBwRzhvGBTsuvi_WOmiTINiok_Wu9F2Z1lD24CCW1t344QbaHNxlV9bHrctRtLHUri11StqsR3BpL9Sjdb4Ff7uNU69D-bbqfPBxTsO-jZ6CNTmeh5RmmGKeP-uXKcAm-XFr8uwMge-0seBiuVh8ooTKp8WjTg8Rnt3_J8XV-3dfFx-ry88fLhbnl5XhVKQKU9YyjTRgthIYZI1Qi4XhgrdUIqDcGMk5lbWmvJO8NpjWRqJOIrISqMb0pHi167sJ_tsEManRRgPDoB34KSosBOeMN434H5Q0VDSSZ_T0D_TaT8HlRe4oVLOa4V9UrwdQ1nU-5QvNTdV5XTOJMSYyU2d_ofJrYbTGO-hszh8IXh8IMpPgNvV6ilFdLL8csnjHmny5GKBTm2BHHbYKIzVbT-2sp7L11Gw9NWte3C83rUZofyr2XssA2QExl1wP4bft_9H15U7Uaa90n12qrpYEYZ7NjBpKCP0ByiDrSg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1772045451</pqid></control><display><type>article</type><title>Characterisation of the genes involved in the biosynthesis and attachment of the aminodeoxysugar D-forosamine in the auricin gene cluster of Streptomyces aureofaciens CCM3239</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Bekeova, Carmen ; Rehakova, Alena ; Feckova, Lubomira ; Vlckova, Silvia ; Novakova, Renata ; Mingyar, Erik ; Kormanec, Jan</creator><creatorcontrib>Bekeova, Carmen ; Rehakova, Alena ; Feckova, Lubomira ; Vlckova, Silvia ; Novakova, Renata ; Mingyar, Erik ; Kormanec, Jan</creatorcontrib><description>We previously identified the aur1 gene cluster which produces the angucycline antibiotic auricin. Preliminary characterisation of auricin revealed that it is modified by a single aminodeoxysugar, D-forosamine. Here we characterise the D-forosamine-specific genes. The four close tandem genes, aur1TQSV, encoding enzymes involved in the initial steps of the deoxysugar biosynthesis, were located on a large operon with other core auricin biosynthetic genes. Deleting these genes resulted in the absence of auricin and the production of deglycosylated auricin intermediates. The two final D-forosamine biosynthetic genes, sa59, an NDP-hexose aminotransferase, and sa52, an NDP-aminohexose N-dimethyltransferase, are located in a region rather distant from the core auricin genes. A deletion analysis of these genes confirmed their role in D-forosamine biosynthesis. The Δsa59 mutant had a phenotype similar to that of the cluster deletion mutant, while the Δsa52 mutant produced an auricin with a demethylated D-forosamine. Although auricin contains a single deoxyhexose, two glycosyltransferase genes were found to participate in the attachment of D-forosamine to the auricin aglycon. An analysis of the expression of the D-forosamine biosynthesis genes revealed that the initial D-forosamine biosynthetic genes aur1TQSV are regulated together with the other auricin core genes by the aur1Ap promoter under the control of the auricin-specific activator Aur1P. The expression of the other D-forosamine genes, however, is governed by promoters differentially dependent upon the two SARP family auricin-specific activators Aur1PR3 and Aur1PR4. These promoters contain direct repeats similar to the SARP consensus sequence and are involved in the interaction with both regulators.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-015-7214-9</identifier><identifier>PMID: 26685675</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Amines ; Anti-Bacterial Agents - biosynthesis ; Antibiotics ; Applied Genetics and Molecular Biotechnology ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Base Sequence ; Biomedical and Life Sciences ; Biosynthesis ; Biotechnology ; consensus sequence ; E coli ; Enzymes ; Gene Deletion ; Gene expression ; Gene Expression Regulation, Bacterial ; Genes ; Genetic aspects ; Hexosamines - biosynthesis ; Life Sciences ; Macrolides - metabolism ; Metabolites ; Microbial Genetics and Genomics ; Microbiology ; Multigene Family ; mutants ; Natural products ; Observations ; Operon ; phenotype ; Plasmids ; Promoter Regions, Genetic ; Secondary Metabolism - genetics ; Sequence Alignment ; Streptomyces ; Streptomyces aureofaciens ; Streptomyces aureofaciens - genetics ; Streptomyces aureofaciens - metabolism ; Studies ; Transaminases - genetics ; Transaminases - metabolism</subject><ispartof>Applied microbiology and biotechnology, 2016-04, Vol.100 (7), p.3177-3195</ispartof><rights>Springer-Verlag Berlin Heidelberg 2015</rights><rights>COPYRIGHT 2016 Springer</rights><rights>Springer-Verlag Berlin Heidelberg 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c637t-135d5a0ae15b71e9400d17c676d390e36cc966394a36f964c134c90f902b70413</citedby><cites>FETCH-LOGICAL-c637t-135d5a0ae15b71e9400d17c676d390e36cc966394a36f964c134c90f902b70413</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00253-015-7214-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00253-015-7214-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26685675$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bekeova, Carmen</creatorcontrib><creatorcontrib>Rehakova, Alena</creatorcontrib><creatorcontrib>Feckova, Lubomira</creatorcontrib><creatorcontrib>Vlckova, Silvia</creatorcontrib><creatorcontrib>Novakova, Renata</creatorcontrib><creatorcontrib>Mingyar, Erik</creatorcontrib><creatorcontrib>Kormanec, Jan</creatorcontrib><title>Characterisation of the genes involved in the biosynthesis and attachment of the aminodeoxysugar D-forosamine in the auricin gene cluster of Streptomyces aureofaciens CCM3239</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><addtitle>Appl Microbiol Biotechnol</addtitle><description>We previously identified the aur1 gene cluster which produces the angucycline antibiotic auricin. Preliminary characterisation of auricin revealed that it is modified by a single aminodeoxysugar, D-forosamine. Here we characterise the D-forosamine-specific genes. The four close tandem genes, aur1TQSV, encoding enzymes involved in the initial steps of the deoxysugar biosynthesis, were located on a large operon with other core auricin biosynthetic genes. Deleting these genes resulted in the absence of auricin and the production of deglycosylated auricin intermediates. The two final D-forosamine biosynthetic genes, sa59, an NDP-hexose aminotransferase, and sa52, an NDP-aminohexose N-dimethyltransferase, are located in a region rather distant from the core auricin genes. A deletion analysis of these genes confirmed their role in D-forosamine biosynthesis. The Δsa59 mutant had a phenotype similar to that of the cluster deletion mutant, while the Δsa52 mutant produced an auricin with a demethylated D-forosamine. Although auricin contains a single deoxyhexose, two glycosyltransferase genes were found to participate in the attachment of D-forosamine to the auricin aglycon. An analysis of the expression of the D-forosamine biosynthesis genes revealed that the initial D-forosamine biosynthetic genes aur1TQSV are regulated together with the other auricin core genes by the aur1Ap promoter under the control of the auricin-specific activator Aur1P. The expression of the other D-forosamine genes, however, is governed by promoters differentially dependent upon the two SARP family auricin-specific activators Aur1PR3 and Aur1PR4. These promoters contain direct repeats similar to the SARP consensus sequence and are involved in the interaction with both regulators.</description><subject>Amines</subject><subject>Anti-Bacterial Agents - biosynthesis</subject><subject>Antibiotics</subject><subject>Applied Genetics and Molecular Biotechnology</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Base Sequence</subject><subject>Biomedical and Life Sciences</subject><subject>Biosynthesis</subject><subject>Biotechnology</subject><subject>consensus sequence</subject><subject>E coli</subject><subject>Enzymes</subject><subject>Gene Deletion</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Hexosamines - biosynthesis</subject><subject>Life Sciences</subject><subject>Macrolides - metabolism</subject><subject>Metabolites</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Multigene Family</subject><subject>mutants</subject><subject>Natural products</subject><subject>Observations</subject><subject>Operon</subject><subject>phenotype</subject><subject>Plasmids</subject><subject>Promoter Regions, Genetic</subject><subject>Secondary Metabolism - genetics</subject><subject>Sequence Alignment</subject><subject>Streptomyces</subject><subject>Streptomyces aureofaciens</subject><subject>Streptomyces aureofaciens - genetics</subject><subject>Streptomyces aureofaciens - metabolism</subject><subject>Studies</subject><subject>Transaminases - genetics</subject><subject>Transaminases - metabolism</subject><issn>0175-7598</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkk1v1DAQhiMEoqXwA7hApF7gkOKP2I6P1fJVqQiJpWfL60yyrhJ7sZ2q-6f4jTjdLrAIIeSDRzPPO6PRvEXxHKMzjJB4ExEijFYIs0oQXFfyQXGMa0oqxHH9sDhGWOQKk81R8STGa4QwaTh_XBwRzhvGBTsuvi_WOmiTINiok_Wu9F2Z1lD24CCW1t344QbaHNxlV9bHrctRtLHUri11StqsR3BpL9Sjdb4Ff7uNU69D-bbqfPBxTsO-jZ6CNTmeh5RmmGKeP-uXKcAm-XFr8uwMge-0seBiuVh8ooTKp8WjTg8Rnt3_J8XV-3dfFx-ry88fLhbnl5XhVKQKU9YyjTRgthIYZI1Qi4XhgrdUIqDcGMk5lbWmvJO8NpjWRqJOIrISqMb0pHi167sJ_tsEManRRgPDoB34KSosBOeMN434H5Q0VDSSZ_T0D_TaT8HlRe4oVLOa4V9UrwdQ1nU-5QvNTdV5XTOJMSYyU2d_ofJrYbTGO-hszh8IXh8IMpPgNvV6ilFdLL8csnjHmny5GKBTm2BHHbYKIzVbT-2sp7L11Gw9NWte3C83rUZofyr2XssA2QExl1wP4bft_9H15U7Uaa90n12qrpYEYZ7NjBpKCP0ByiDrSg</recordid><startdate>20160401</startdate><enddate>20160401</enddate><creator>Bekeova, Carmen</creator><creator>Rehakova, Alena</creator><creator>Feckova, Lubomira</creator><creator>Vlckova, Silvia</creator><creator>Novakova, Renata</creator><creator>Mingyar, Erik</creator><creator>Kormanec, Jan</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>LK8</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>7QO</scope></search><sort><creationdate>20160401</creationdate><title>Characterisation of the genes involved in the biosynthesis and attachment of the aminodeoxysugar D-forosamine in the auricin gene cluster of Streptomyces aureofaciens CCM3239</title><author>Bekeova, Carmen ; Rehakova, Alena ; Feckova, Lubomira ; Vlckova, Silvia ; Novakova, Renata ; Mingyar, Erik ; Kormanec, Jan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c637t-135d5a0ae15b71e9400d17c676d390e36cc966394a36f964c134c90f902b70413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Amines</topic><topic>Anti-Bacterial Agents - biosynthesis</topic><topic>Antibiotics</topic><topic>Applied Genetics and Molecular Biotechnology</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Base Sequence</topic><topic>Biomedical and Life Sciences</topic><topic>Biosynthesis</topic><topic>Biotechnology</topic><topic>consensus sequence</topic><topic>E coli</topic><topic>Enzymes</topic><topic>Gene Deletion</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Hexosamines - biosynthesis</topic><topic>Life Sciences</topic><topic>Macrolides - metabolism</topic><topic>Metabolites</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Multigene Family</topic><topic>mutants</topic><topic>Natural products</topic><topic>Observations</topic><topic>Operon</topic><topic>phenotype</topic><topic>Plasmids</topic><topic>Promoter Regions, Genetic</topic><topic>Secondary Metabolism - genetics</topic><topic>Sequence Alignment</topic><topic>Streptomyces</topic><topic>Streptomyces aureofaciens</topic><topic>Streptomyces aureofaciens - genetics</topic><topic>Streptomyces aureofaciens - metabolism</topic><topic>Studies</topic><topic>Transaminases - genetics</topic><topic>Transaminases - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bekeova, Carmen</creatorcontrib><creatorcontrib>Rehakova, Alena</creatorcontrib><creatorcontrib>Feckova, Lubomira</creatorcontrib><creatorcontrib>Vlckova, Silvia</creatorcontrib><creatorcontrib>Novakova, Renata</creatorcontrib><creatorcontrib>Mingyar, Erik</creatorcontrib><creatorcontrib>Kormanec, Jan</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>ABI/INFORM Collection</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Global (Alumni Edition)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>ProQuest Biological Science Collection</collection><collection>ABI/INFORM Global</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Business</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><jtitle>Applied microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bekeova, Carmen</au><au>Rehakova, Alena</au><au>Feckova, Lubomira</au><au>Vlckova, Silvia</au><au>Novakova, Renata</au><au>Mingyar, Erik</au><au>Kormanec, Jan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterisation of the genes involved in the biosynthesis and attachment of the aminodeoxysugar D-forosamine in the auricin gene cluster of Streptomyces aureofaciens CCM3239</atitle><jtitle>Applied microbiology and biotechnology</jtitle><stitle>Appl Microbiol Biotechnol</stitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2016-04-01</date><risdate>2016</risdate><volume>100</volume><issue>7</issue><spage>3177</spage><epage>3195</epage><pages>3177-3195</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><abstract>We previously identified the aur1 gene cluster which produces the angucycline antibiotic auricin. Preliminary characterisation of auricin revealed that it is modified by a single aminodeoxysugar, D-forosamine. Here we characterise the D-forosamine-specific genes. The four close tandem genes, aur1TQSV, encoding enzymes involved in the initial steps of the deoxysugar biosynthesis, were located on a large operon with other core auricin biosynthetic genes. Deleting these genes resulted in the absence of auricin and the production of deglycosylated auricin intermediates. The two final D-forosamine biosynthetic genes, sa59, an NDP-hexose aminotransferase, and sa52, an NDP-aminohexose N-dimethyltransferase, are located in a region rather distant from the core auricin genes. A deletion analysis of these genes confirmed their role in D-forosamine biosynthesis. The Δsa59 mutant had a phenotype similar to that of the cluster deletion mutant, while the Δsa52 mutant produced an auricin with a demethylated D-forosamine. Although auricin contains a single deoxyhexose, two glycosyltransferase genes were found to participate in the attachment of D-forosamine to the auricin aglycon. An analysis of the expression of the D-forosamine biosynthesis genes revealed that the initial D-forosamine biosynthetic genes aur1TQSV are regulated together with the other auricin core genes by the aur1Ap promoter under the control of the auricin-specific activator Aur1P. The expression of the other D-forosamine genes, however, is governed by promoters differentially dependent upon the two SARP family auricin-specific activators Aur1PR3 and Aur1PR4. These promoters contain direct repeats similar to the SARP consensus sequence and are involved in the interaction with both regulators.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>26685675</pmid><doi>10.1007/s00253-015-7214-9</doi><tpages>19</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0175-7598 |
| ispartof | Applied microbiology and biotechnology, 2016-04, Vol.100 (7), p.3177-3195 |
| issn | 0175-7598 1432-0614 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1776656887 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Amines Anti-Bacterial Agents - biosynthesis Antibiotics Applied Genetics and Molecular Biotechnology Bacterial Proteins - genetics Bacterial Proteins - metabolism Base Sequence Biomedical and Life Sciences Biosynthesis Biotechnology consensus sequence E coli Enzymes Gene Deletion Gene expression Gene Expression Regulation, Bacterial Genes Genetic aspects Hexosamines - biosynthesis Life Sciences Macrolides - metabolism Metabolites Microbial Genetics and Genomics Microbiology Multigene Family mutants Natural products Observations Operon phenotype Plasmids Promoter Regions, Genetic Secondary Metabolism - genetics Sequence Alignment Streptomyces Streptomyces aureofaciens Streptomyces aureofaciens - genetics Streptomyces aureofaciens - metabolism Studies Transaminases - genetics Transaminases - metabolism |
| title | Characterisation of the genes involved in the biosynthesis and attachment of the aminodeoxysugar D-forosamine in the auricin gene cluster of Streptomyces aureofaciens CCM3239 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T20%3A23%3A26IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characterisation%20of%20the%20genes%20involved%20in%20the%20biosynthesis%20and%20attachment%20of%20the%20aminodeoxysugar%20D-forosamine%20in%20the%20auricin%20gene%20cluster%20of%20Streptomyces%20aureofaciens%20CCM3239&rft.jtitle=Applied%20microbiology%20and%20biotechnology&rft.au=Bekeova,%20Carmen&rft.date=2016-04-01&rft.volume=100&rft.issue=7&rft.spage=3177&rft.epage=3195&rft.pages=3177-3195&rft.issn=0175-7598&rft.eissn=1432-0614&rft_id=info:doi/10.1007/s00253-015-7214-9&rft_dat=%3Cgale_proqu%3EA445911129%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1772045451&rft_id=info:pmid/26685675&rft_galeid=A445911129&rfr_iscdi=true |