Association between MHC II beta chain gene polymorphisms and resistance to infectious haematopoietic necrosis virus in rainbow trout (Oncorhynchus mykiss, Walbaum, 1792)
Infectious haematopoietic necrosis virus (IHNV) is a serious pathogenic threat to rainbow trout (Oncorhynchus mykiss) and other salmonids. Major histocompatibility complex (MHC) polymorphisms have been closely linked to disease susceptibility. In this study, polymerase chain reaction‐single‐strand c...
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| Veröffentlicht in: | Aquaculture research 2016-02, Vol.47 (2), p.570-578 |
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| creator | Yang, Juan Liu, Zhe Shi, Hai‐Na Zhang, Jiu‐Pan Wang, Jian‐Fu Huang, Jin‐Qiang Kang, Yu‐Jun |
| description | Infectious haematopoietic necrosis virus (IHNV) is a serious pathogenic threat to rainbow trout (Oncorhynchus mykiss) and other salmonids. Major histocompatibility complex (MHC) polymorphisms have been closely linked to disease susceptibility. In this study, polymerase chain reaction‐single‐strand conformation polymorphism, cloning and sequencing were used to examine MHC class II β chain gene (DAB) polymorphisms and analyse their association with IHNV in rainbow trout. The DAB exon 2 was amplified from 104 resistant and 91 susceptible fish. Forty‐five different alleles were identified, 30 of which were novel. Individuals possessed between one and four alleles, indicating that the genome harbours at least two closely related DAB exon 2 loci. The percentages of amino acid mutations in the peptide binding region (PBR) and non‐PBR were 66.67% and 45.28% respectively. The rate of non‐synonymous substitutions occurred at a significantly higher frequency than synonymous substitutions, suggesting the existence of balancing selection for maintenance of polymorphisms at the DAB exon 2 locus. The alleles Onmy‐DAB*0201 (P |
| doi_str_mv | 10.1111/are.12516 |
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Major histocompatibility complex (MHC) polymorphisms have been closely linked to disease susceptibility. In this study, polymerase chain reaction‐single‐strand conformation polymorphism, cloning and sequencing were used to examine MHC class II β chain gene (DAB) polymorphisms and analyse their association with IHNV in rainbow trout. The DAB exon 2 was amplified from 104 resistant and 91 susceptible fish. Forty‐five different alleles were identified, 30 of which were novel. Individuals possessed between one and four alleles, indicating that the genome harbours at least two closely related DAB exon 2 loci. The percentages of amino acid mutations in the peptide binding region (PBR) and non‐PBR were 66.67% and 45.28% respectively. The rate of non‐synonymous substitutions occurred at a significantly higher frequency than synonymous substitutions, suggesting the existence of balancing selection for maintenance of polymorphisms at the DAB exon 2 locus. The alleles Onmy‐DAB*0201 (P < 0.001), Onmy‐DAB*0904 (P < 0.001) and Onmy‐DAB*1102 (P < 0.01) were associated with susceptibility to IHNV, while Onmy‐DAB*0103, Onmy‐DAB*0601 and Onmy‐DAB*1402 were associated with resistance (P < 0.001). These alleles could be used as molecular markers for IHNV resistance breeding in rainbow trout.</description><identifier>ISSN: 1355-557X</identifier><identifier>EISSN: 1365-2109</identifier><identifier>DOI: 10.1111/are.12516</identifier><language>eng</language><publisher>Oxford: Blackwell Science</publisher><subject>alleles ; amino acids ; breeding ; disease resistance ; exons ; fish ; genetic markers ; genetic polymorphism ; Infectious haematopoietic necrosis virus ; Infectious hematopoietic necrosis virus ; loci ; major histocompatibility complex ; MHC class II β chian genes ; Oncorhynchus mykiss ; polymorphism ; resistance</subject><ispartof>Aquaculture research, 2016-02, Vol.47 (2), p.570-578</ispartof><rights>2014 John Wiley & Sons Ltd</rights><rights>Copyright © 2016 John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4976-db9ed99d239ad1ed9431b4aaab7392c83c0a99652465d77a2ad8882bc2cad90b3</citedby><cites>FETCH-LOGICAL-c4976-db9ed99d239ad1ed9431b4aaab7392c83c0a99652465d77a2ad8882bc2cad90b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fare.12516$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fare.12516$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids></links><search><creatorcontrib>Yang, Juan</creatorcontrib><creatorcontrib>Liu, Zhe</creatorcontrib><creatorcontrib>Shi, Hai‐Na</creatorcontrib><creatorcontrib>Zhang, Jiu‐Pan</creatorcontrib><creatorcontrib>Wang, Jian‐Fu</creatorcontrib><creatorcontrib>Huang, Jin‐Qiang</creatorcontrib><creatorcontrib>Kang, Yu‐Jun</creatorcontrib><title>Association between MHC II beta chain gene polymorphisms and resistance to infectious haematopoietic necrosis virus in rainbow trout (Oncorhynchus mykiss, Walbaum, 1792)</title><title>Aquaculture research</title><addtitle>Aquac Res</addtitle><description>Infectious haematopoietic necrosis virus (IHNV) is a serious pathogenic threat to rainbow trout (Oncorhynchus mykiss) and other salmonids. Major histocompatibility complex (MHC) polymorphisms have been closely linked to disease susceptibility. In this study, polymerase chain reaction‐single‐strand conformation polymorphism, cloning and sequencing were used to examine MHC class II β chain gene (DAB) polymorphisms and analyse their association with IHNV in rainbow trout. The DAB exon 2 was amplified from 104 resistant and 91 susceptible fish. Forty‐five different alleles were identified, 30 of which were novel. Individuals possessed between one and four alleles, indicating that the genome harbours at least two closely related DAB exon 2 loci. The percentages of amino acid mutations in the peptide binding region (PBR) and non‐PBR were 66.67% and 45.28% respectively. The rate of non‐synonymous substitutions occurred at a significantly higher frequency than synonymous substitutions, suggesting the existence of balancing selection for maintenance of polymorphisms at the DAB exon 2 locus. The alleles Onmy‐DAB*0201 (P < 0.001), Onmy‐DAB*0904 (P < 0.001) and Onmy‐DAB*1102 (P < 0.01) were associated with susceptibility to IHNV, while Onmy‐DAB*0103, Onmy‐DAB*0601 and Onmy‐DAB*1402 were associated with resistance (P < 0.001). These alleles could be used as molecular markers for IHNV resistance breeding in rainbow trout.</description><subject>alleles</subject><subject>amino acids</subject><subject>breeding</subject><subject>disease resistance</subject><subject>exons</subject><subject>fish</subject><subject>genetic markers</subject><subject>genetic polymorphism</subject><subject>Infectious haematopoietic necrosis virus</subject><subject>Infectious hematopoietic necrosis virus</subject><subject>loci</subject><subject>major histocompatibility complex</subject><subject>MHC class II β chian genes</subject><subject>Oncorhynchus mykiss</subject><subject>polymorphism</subject><subject>resistance</subject><issn>1355-557X</issn><issn>1365-2109</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNp1kdFu0zAUhiMEEqNwwRNgiZtNWjbbieP6sqtGV6kwtDGNO-vEcRdviZ3ZCV0eaW-JS4ALJCxZPtb5_l_H_pPkPcEnJK5T8PqEUEaKF8kByQqWUoLFy33NWMoY__46eRPCPcYkxxk5SJ4XIThloDfOolL3O60t-nyxROv1_gpI1WAsutNWo841Y-t8V5vQBgS2Ql4HE3qwSqPeIWO3WkWjIaAadAu965zRvVHIauVdRNEP42M3GvroWrod6r0benR4aZXz9WhVHdvt-GBCOEa30JQwtMeIcEGP3iavttAE_e73OUtuPp1_W16km8vVernYpCoXvEirUuhKiIpmAioSyzwjZQ4AJc8EVfNMYRCiYDQvWMU5UKjm8zktFVVQCVxms-Rw8u28exx06GVrgtJNA1bHp0nCeVEwwimJ6Md_0Hs3eBunixTLaJwh7llyNFH7Pwheb2XnTQt-lATLfWgyhiZ_hRbZ04ndmUaP_wfl4ur8jyKdFDEI_fRXAf5BFjzjTN5-Wcmv5Oxqc7a6liLyHyZ-C07CnTdB3lxTTAqMsaCcFdlP_1izRA</recordid><startdate>201602</startdate><enddate>201602</enddate><creator>Yang, Juan</creator><creator>Liu, Zhe</creator><creator>Shi, Hai‐Na</creator><creator>Zhang, Jiu‐Pan</creator><creator>Wang, Jian‐Fu</creator><creator>Huang, Jin‐Qiang</creator><creator>Kang, Yu‐Jun</creator><general>Blackwell Science</general><general>Blackwell Publishing Ltd</general><general>Hindawi Limited</general><scope>FBQ</scope><scope>BSCLL</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TN</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>201602</creationdate><title>Association between MHC II beta chain gene polymorphisms and resistance to infectious haematopoietic necrosis virus in rainbow trout (Oncorhynchus mykiss, Walbaum, 1792)</title><author>Yang, Juan ; Liu, Zhe ; Shi, Hai‐Na ; Zhang, Jiu‐Pan ; Wang, Jian‐Fu ; Huang, Jin‐Qiang ; Kang, Yu‐Jun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4976-db9ed99d239ad1ed9431b4aaab7392c83c0a99652465d77a2ad8882bc2cad90b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>alleles</topic><topic>amino acids</topic><topic>breeding</topic><topic>disease resistance</topic><topic>exons</topic><topic>fish</topic><topic>genetic markers</topic><topic>genetic polymorphism</topic><topic>Infectious haematopoietic necrosis virus</topic><topic>Infectious hematopoietic necrosis virus</topic><topic>loci</topic><topic>major histocompatibility complex</topic><topic>MHC class II β chian genes</topic><topic>Oncorhynchus mykiss</topic><topic>polymorphism</topic><topic>resistance</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Juan</creatorcontrib><creatorcontrib>Liu, Zhe</creatorcontrib><creatorcontrib>Shi, Hai‐Na</creatorcontrib><creatorcontrib>Zhang, Jiu‐Pan</creatorcontrib><creatorcontrib>Wang, Jian‐Fu</creatorcontrib><creatorcontrib>Huang, Jin‐Qiang</creatorcontrib><creatorcontrib>Kang, Yu‐Jun</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>CrossRef</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Aquaculture research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Juan</au><au>Liu, Zhe</au><au>Shi, Hai‐Na</au><au>Zhang, Jiu‐Pan</au><au>Wang, Jian‐Fu</au><au>Huang, Jin‐Qiang</au><au>Kang, Yu‐Jun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Association between MHC II beta chain gene polymorphisms and resistance to infectious haematopoietic necrosis virus in rainbow trout (Oncorhynchus mykiss, Walbaum, 1792)</atitle><jtitle>Aquaculture research</jtitle><addtitle>Aquac Res</addtitle><date>2016-02</date><risdate>2016</risdate><volume>47</volume><issue>2</issue><spage>570</spage><epage>578</epage><pages>570-578</pages><issn>1355-557X</issn><eissn>1365-2109</eissn><abstract>Infectious haematopoietic necrosis virus (IHNV) is a serious pathogenic threat to rainbow trout (Oncorhynchus mykiss) and other salmonids. Major histocompatibility complex (MHC) polymorphisms have been closely linked to disease susceptibility. In this study, polymerase chain reaction‐single‐strand conformation polymorphism, cloning and sequencing were used to examine MHC class II β chain gene (DAB) polymorphisms and analyse their association with IHNV in rainbow trout. The DAB exon 2 was amplified from 104 resistant and 91 susceptible fish. Forty‐five different alleles were identified, 30 of which were novel. Individuals possessed between one and four alleles, indicating that the genome harbours at least two closely related DAB exon 2 loci. The percentages of amino acid mutations in the peptide binding region (PBR) and non‐PBR were 66.67% and 45.28% respectively. The rate of non‐synonymous substitutions occurred at a significantly higher frequency than synonymous substitutions, suggesting the existence of balancing selection for maintenance of polymorphisms at the DAB exon 2 locus. The alleles Onmy‐DAB*0201 (P < 0.001), Onmy‐DAB*0904 (P < 0.001) and Onmy‐DAB*1102 (P < 0.01) were associated with susceptibility to IHNV, while Onmy‐DAB*0103, Onmy‐DAB*0601 and Onmy‐DAB*1402 were associated with resistance (P < 0.001). These alleles could be used as molecular markers for IHNV resistance breeding in rainbow trout.</abstract><cop>Oxford</cop><pub>Blackwell Science</pub><doi>10.1111/are.12516</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | alleles amino acids breeding disease resistance exons fish genetic markers genetic polymorphism Infectious haematopoietic necrosis virus Infectious hematopoietic necrosis virus loci major histocompatibility complex MHC class II β chian genes Oncorhynchus mykiss polymorphism resistance |
| title | Association between MHC II beta chain gene polymorphisms and resistance to infectious haematopoietic necrosis virus in rainbow trout (Oncorhynchus mykiss, Walbaum, 1792) |
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