Influence of high voltage atmospheric cold plasma process parameters and role of relative humidity on inactivation of Bacillus atrophaeus spores inside a sealed package
Summary Background Non-thermal plasma has received much attention for elimination of microbial contamination from a range of surfaces. Aim This study aimed to determine the effect of a range of dielectric barrier discharge high voltage atmospheric cold plasma (HVACP) parameters for inactivation of B...
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description | Summary Background Non-thermal plasma has received much attention for elimination of microbial contamination from a range of surfaces. Aim This study aimed to determine the effect of a range of dielectric barrier discharge high voltage atmospheric cold plasma (HVACP) parameters for inactivation of Bacillus atrophaeus spores inside a sealed package. Methods A sterile polystyrene Petri dish containing B. atrophaeus spore strip (spore population 2.3 × 106 /strip i.e. 6.36 log10 /strip) was placed in a sealed polypropylene container and was subjected to HVACP treatment. The HVACP discharge was generated between two aluminium plate electrodes using a high voltage of 70 kVRMS . The effects of process parameters, including treatment time, mode of exposure (direct/indirect), and working gas types, were evaluated. The influence of relative humidity on HVACP inactivation efficacy was also assessed. The inactivation efficacy was evaluated using colony counts. Optical absorption spectroscopy (OAS) was used to assess gas composition following HVACP exposure. Findings A strong effect of process parameters on inactivation was observed. Direct plasma exposure for 60 s resulted in ≥6 log10 cycle reduction of spores in all gas types tested. However, indirect exposure for 60 s resulted in either 2.1 or 6.3 log10 cycle reduction of spores depending on gas types used for HVACP generation. The relative humidity (RH) was a critical factor in bacterial spore inactivation by HVACP, where a major role of plasma-generated species other than ozone was noted. Direct and indirect HVACP exposure for 60 s at 70% RH recorded 6.3 and 5.7 log10 cycle reduction of spores, respectively. Conclusion In summary, a strong influence of process parameters on spore inactivation was noted. Rapid in-package HVACP inactivation of bacterial spores within 30–60 s demonstrates the promising potential application for reduction of spores on medical devices and heat-sensitive materials. |
doi_str_mv | 10.1016/j.jhin.2014.08.009 |
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Aim This study aimed to determine the effect of a range of dielectric barrier discharge high voltage atmospheric cold plasma (HVACP) parameters for inactivation of Bacillus atrophaeus spores inside a sealed package. Methods A sterile polystyrene Petri dish containing B. atrophaeus spore strip (spore population 2.3 × 106 /strip i.e. 6.36 log10 /strip) was placed in a sealed polypropylene container and was subjected to HVACP treatment. The HVACP discharge was generated between two aluminium plate electrodes using a high voltage of 70 kVRMS . The effects of process parameters, including treatment time, mode of exposure (direct/indirect), and working gas types, were evaluated. The influence of relative humidity on HVACP inactivation efficacy was also assessed. The inactivation efficacy was evaluated using colony counts. Optical absorption spectroscopy (OAS) was used to assess gas composition following HVACP exposure. Findings A strong effect of process parameters on inactivation was observed. Direct plasma exposure for 60 s resulted in ≥6 log10 cycle reduction of spores in all gas types tested. However, indirect exposure for 60 s resulted in either 2.1 or 6.3 log10 cycle reduction of spores depending on gas types used for HVACP generation. The relative humidity (RH) was a critical factor in bacterial spore inactivation by HVACP, where a major role of plasma-generated species other than ozone was noted. Direct and indirect HVACP exposure for 60 s at 70% RH recorded 6.3 and 5.7 log10 cycle reduction of spores, respectively. Conclusion In summary, a strong influence of process parameters on spore inactivation was noted. Rapid in-package HVACP inactivation of bacterial spores within 30–60 s demonstrates the promising potential application for reduction of spores on medical devices and heat-sensitive materials.</description><identifier>ISSN: 0195-6701</identifier><identifier>EISSN: 1532-2939</identifier><identifier>DOI: 10.1016/j.jhin.2014.08.009</identifier><identifier>PMID: 25308932</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Bacillus ; Bacillus - growth & development ; Bacillus - physiology ; Bacillus atrophaeus ; Biological and medical sciences ; Cold Temperature ; Gas types ; High voltage atmospheric cold plasma ; Human viral diseases ; Humidity ; Inactivation ; Infectious Disease ; Infectious diseases ; Medical sciences ; Microbial Viability ; Optical absorption spectroscopy ; Plasma Gases - adverse effects ; Relative humidity ; Spores, Bacterial ; Viral diseases ; Viral diseases of the respiratory system and ent viral diseases</subject><ispartof>The Journal of hospital infection, 2014-11, Vol.88 (3), p.162-169</ispartof><rights>The Healthcare Infection Society</rights><rights>2014 The Healthcare Infection Society</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2014 The Healthcare Infection Society. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c540t-51af298403fa56e9e020abb02f6e534b859c0bbf0a91909b09ea79bdb20ac1263</citedby><cites>FETCH-LOGICAL-c540t-51af298403fa56e9e020abb02f6e534b859c0bbf0a91909b09ea79bdb20ac1263</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jhin.2014.08.009$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28938072$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25308932$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Patil, S</creatorcontrib><creatorcontrib>Moiseev, T</creatorcontrib><creatorcontrib>Misra, N.N</creatorcontrib><creatorcontrib>Cullen, P.J</creatorcontrib><creatorcontrib>Mosnier, J.P</creatorcontrib><creatorcontrib>Keener, K.M</creatorcontrib><creatorcontrib>Bourke, P</creatorcontrib><title>Influence of high voltage atmospheric cold plasma process parameters and role of relative humidity on inactivation of Bacillus atrophaeus spores inside a sealed package</title><title>The Journal of hospital infection</title><addtitle>J Hosp Infect</addtitle><description>Summary Background Non-thermal plasma has received much attention for elimination of microbial contamination from a range of surfaces. Aim This study aimed to determine the effect of a range of dielectric barrier discharge high voltage atmospheric cold plasma (HVACP) parameters for inactivation of Bacillus atrophaeus spores inside a sealed package. Methods A sterile polystyrene Petri dish containing B. atrophaeus spore strip (spore population 2.3 × 106 /strip i.e. 6.36 log10 /strip) was placed in a sealed polypropylene container and was subjected to HVACP treatment. The HVACP discharge was generated between two aluminium plate electrodes using a high voltage of 70 kVRMS . The effects of process parameters, including treatment time, mode of exposure (direct/indirect), and working gas types, were evaluated. The influence of relative humidity on HVACP inactivation efficacy was also assessed. The inactivation efficacy was evaluated using colony counts. Optical absorption spectroscopy (OAS) was used to assess gas composition following HVACP exposure. Findings A strong effect of process parameters on inactivation was observed. Direct plasma exposure for 60 s resulted in ≥6 log10 cycle reduction of spores in all gas types tested. However, indirect exposure for 60 s resulted in either 2.1 or 6.3 log10 cycle reduction of spores depending on gas types used for HVACP generation. The relative humidity (RH) was a critical factor in bacterial spore inactivation by HVACP, where a major role of plasma-generated species other than ozone was noted. Direct and indirect HVACP exposure for 60 s at 70% RH recorded 6.3 and 5.7 log10 cycle reduction of spores, respectively. Conclusion In summary, a strong influence of process parameters on spore inactivation was noted. Rapid in-package HVACP inactivation of bacterial spores within 30–60 s demonstrates the promising potential application for reduction of spores on medical devices and heat-sensitive materials.</description><subject>Bacillus</subject><subject>Bacillus - growth & development</subject><subject>Bacillus - physiology</subject><subject>Bacillus atrophaeus</subject><subject>Biological and medical sciences</subject><subject>Cold Temperature</subject><subject>Gas types</subject><subject>High voltage atmospheric cold plasma</subject><subject>Human viral diseases</subject><subject>Humidity</subject><subject>Inactivation</subject><subject>Infectious Disease</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Microbial Viability</subject><subject>Optical absorption spectroscopy</subject><subject>Plasma Gases - adverse effects</subject><subject>Relative humidity</subject><subject>Spores, Bacterial</subject><subject>Viral diseases</subject><subject>Viral diseases of the respiratory system and ent viral diseases</subject><issn>0195-6701</issn><issn>1532-2939</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks-O1DAMxisEYpeBF-CAckHiMoOTNm0jISRY8WellTgA58hN3W1m06Yk7UjzRjwmKTOAxAFOSZyfPzv5nGVPOew48PLlfrfv7bgTwIsd1DsAdS-75DIXW6FydT-7BK7ktqyAX2SPYtxDInIlH2YXQuZQq1xcZt-vx84tNBpivmO9ve3ZwbsZb4nhPPg49RSsYca7lk0O44BsCt5QjGzCgAPNFCLDsWXBu58agRzO9kCsXwbb2vnI_MjsiCYF00U6JOgtGuvckjLn4KceKW3j5APFhEbbpuosEjpKVdHcpXYeZw86dJGenNdN9vX9uy9XH7c3nz5cX7252RpZwLyVHDuh6gLyDmVJikAANg2IriSZF00tlYGm6QAVV6AaUISVatomYYaLMt9kL0666ZnfFoqzHmw05ByO5JeoeVWVZVEn2f-jpVCqkqBkQsUJNcHHGKjTU7ADhqPmoFcz9V6vZurVTA21Xq3aZM_O-kszUPs75Zd7CXh-BjAadF3A0dj4h0tQDdXKvTpxlD7uYCnoaOzqeWsDmVm33v67j9d_pRtnR5sq3tGR4t4vYUyWaK6j0KA_r2O3Th0vAETFIf8BHWPVfg</recordid><startdate>20141101</startdate><enddate>20141101</enddate><creator>Patil, S</creator><creator>Moiseev, T</creator><creator>Misra, N.N</creator><creator>Cullen, P.J</creator><creator>Mosnier, J.P</creator><creator>Keener, K.M</creator><creator>Bourke, P</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>20141101</creationdate><title>Influence of high voltage atmospheric cold plasma process parameters and role of relative humidity on inactivation of Bacillus atrophaeus spores inside a sealed package</title><author>Patil, S ; Moiseev, T ; Misra, N.N ; Cullen, P.J ; Mosnier, J.P ; Keener, K.M ; Bourke, P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c540t-51af298403fa56e9e020abb02f6e534b859c0bbf0a91909b09ea79bdb20ac1263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Bacillus</topic><topic>Bacillus - growth & development</topic><topic>Bacillus - physiology</topic><topic>Bacillus atrophaeus</topic><topic>Biological and medical sciences</topic><topic>Cold Temperature</topic><topic>Gas types</topic><topic>High voltage atmospheric cold plasma</topic><topic>Human viral diseases</topic><topic>Humidity</topic><topic>Inactivation</topic><topic>Infectious Disease</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Microbial Viability</topic><topic>Optical absorption spectroscopy</topic><topic>Plasma Gases - adverse effects</topic><topic>Relative humidity</topic><topic>Spores, Bacterial</topic><topic>Viral diseases</topic><topic>Viral diseases of the respiratory system and ent viral diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Patil, S</creatorcontrib><creatorcontrib>Moiseev, T</creatorcontrib><creatorcontrib>Misra, N.N</creatorcontrib><creatorcontrib>Cullen, P.J</creatorcontrib><creatorcontrib>Mosnier, J.P</creatorcontrib><creatorcontrib>Keener, K.M</creatorcontrib><creatorcontrib>Bourke, P</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>The Journal of hospital infection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Patil, S</au><au>Moiseev, T</au><au>Misra, N.N</au><au>Cullen, P.J</au><au>Mosnier, J.P</au><au>Keener, K.M</au><au>Bourke, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of high voltage atmospheric cold plasma process parameters and role of relative humidity on inactivation of Bacillus atrophaeus spores inside a sealed package</atitle><jtitle>The Journal of hospital infection</jtitle><addtitle>J Hosp Infect</addtitle><date>2014-11-01</date><risdate>2014</risdate><volume>88</volume><issue>3</issue><spage>162</spage><epage>169</epage><pages>162-169</pages><issn>0195-6701</issn><eissn>1532-2939</eissn><abstract>Summary Background Non-thermal plasma has received much attention for elimination of microbial contamination from a range of surfaces. Aim This study aimed to determine the effect of a range of dielectric barrier discharge high voltage atmospheric cold plasma (HVACP) parameters for inactivation of Bacillus atrophaeus spores inside a sealed package. Methods A sterile polystyrene Petri dish containing B. atrophaeus spore strip (spore population 2.3 × 106 /strip i.e. 6.36 log10 /strip) was placed in a sealed polypropylene container and was subjected to HVACP treatment. The HVACP discharge was generated between two aluminium plate electrodes using a high voltage of 70 kVRMS . The effects of process parameters, including treatment time, mode of exposure (direct/indirect), and working gas types, were evaluated. The influence of relative humidity on HVACP inactivation efficacy was also assessed. The inactivation efficacy was evaluated using colony counts. Optical absorption spectroscopy (OAS) was used to assess gas composition following HVACP exposure. Findings A strong effect of process parameters on inactivation was observed. Direct plasma exposure for 60 s resulted in ≥6 log10 cycle reduction of spores in all gas types tested. However, indirect exposure for 60 s resulted in either 2.1 or 6.3 log10 cycle reduction of spores depending on gas types used for HVACP generation. The relative humidity (RH) was a critical factor in bacterial spore inactivation by HVACP, where a major role of plasma-generated species other than ozone was noted. Direct and indirect HVACP exposure for 60 s at 70% RH recorded 6.3 and 5.7 log10 cycle reduction of spores, respectively. Conclusion In summary, a strong influence of process parameters on spore inactivation was noted. Rapid in-package HVACP inactivation of bacterial spores within 30–60 s demonstrates the promising potential application for reduction of spores on medical devices and heat-sensitive materials.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>25308932</pmid><doi>10.1016/j.jhin.2014.08.009</doi><tpages>8</tpages></addata></record> |
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subjects | Bacillus Bacillus - growth & development Bacillus - physiology Bacillus atrophaeus Biological and medical sciences Cold Temperature Gas types High voltage atmospheric cold plasma Human viral diseases Humidity Inactivation Infectious Disease Infectious diseases Medical sciences Microbial Viability Optical absorption spectroscopy Plasma Gases - adverse effects Relative humidity Spores, Bacterial Viral diseases Viral diseases of the respiratory system and ent viral diseases |
title | Influence of high voltage atmospheric cold plasma process parameters and role of relative humidity on inactivation of Bacillus atrophaeus spores inside a sealed package |
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