Fluid-induced low shear stress improves cartilage like tissue fabrication by encapsulating chondrocytes
In the recent years, there has been considerable development in the regenerative medicine, which aims to repair, regenerate, and improve injured articular cartilage. The aim of the present study was to investigate the effect of flow-induced shear stress in perfusion bioreactor on alginate encapsulat...
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| creator | Gharravi, Anneh Mohammad Orazizadeh, Mahmoud Hashemitabar, Mahmoud |
| description | In the recent years, there has been considerable development in the regenerative medicine, which aims to repair, regenerate, and improve injured articular cartilage. The aim of the present study was to investigate the effect of flow-induced shear stress in perfusion bioreactor on alginate encapsulating chondrocytes. The shear stress imposed on the cells in the culture chamber of bioreactor was predicted with computational fluid dynamic. Bovine nasal chondrocytes were isolated and expanded to obtain a pellet. The cell pellet was resuspends in alginate solution, transferred to the culture chamber, and dynamically cultured under direct perfusion. At the end of culture, tissue constructs were examined histologically and by immunohistochemistry. The results of computational fluid dynamic modeling revealed that maximum wall shear stress was 4.820 × 10
−3
Pascal. Macroscopic views of the alginate/chondrocyte beads suggested that it possessed constant shape but were flexible. Under inverted microscope, round shape of chondrocyte observed. Cell distribution was homogeneous throughout the scaffold. Tissue construct subjected to shear showed morphological features, which are characteristic for natural cartilage. Immunohistochemistry results revealed immunopositivity for type II collagens in tissue constructs samples. Flow induced shear stress in the perfusion bioreactor and chnondrocyte encapsulation provide environment to support cell growth, and tissue regeneration and improve cartilage like tissue fabrication. |
| doi_str_mv | 10.1007/s10561-015-9529-2 |
| format | Article |
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−3
Pascal. Macroscopic views of the alginate/chondrocyte beads suggested that it possessed constant shape but were flexible. Under inverted microscope, round shape of chondrocyte observed. Cell distribution was homogeneous throughout the scaffold. Tissue construct subjected to shear showed morphological features, which are characteristic for natural cartilage. Immunohistochemistry results revealed immunopositivity for type II collagens in tissue constructs samples. Flow induced shear stress in the perfusion bioreactor and chnondrocyte encapsulation provide environment to support cell growth, and tissue regeneration and improve cartilage like tissue fabrication.</description><identifier>ISSN: 1389-9333</identifier><identifier>EISSN: 1573-6814</identifier><identifier>DOI: 10.1007/s10561-015-9529-2</identifier><identifier>PMID: 26254592</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Alginates - pharmacology ; Animals ; Biomedical and Life Sciences ; Biomedicine ; Cartilage, Articular - drug effects ; Cartilage, Articular - physiology ; Cattle ; Cell Biology ; Cells, Cultured ; Cells, Immobilized - cytology ; Chondrocytes - cytology ; Chondrocytes - drug effects ; Chondrocytes - metabolism ; Fluid dynamics ; Glucuronic Acid - pharmacology ; Hexuronic Acids - pharmacology ; Hydrodynamics ; Immunohistochemistry ; Life Sciences ; Membrane reactors ; Microspheres ; Original Paper ; Rheology ; Shear stress ; Stress, Mechanical ; Tissue engineering ; Tissue Engineering - methods ; Tissue Scaffolds - chemistry ; Transplant Surgery</subject><ispartof>Cell and tissue banking, 2016-03, Vol.17 (1), p.117-122</ispartof><rights>Springer Science+Business Media Dordrecht 2015</rights><rights>Springer Science+Business Media Dordrecht 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-243a85d9362d90a52ccf1f17d291c80be042a56317d85497967903f84b6541473</citedby><cites>FETCH-LOGICAL-c475t-243a85d9362d90a52ccf1f17d291c80be042a56317d85497967903f84b6541473</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10561-015-9529-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10561-015-9529-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26254592$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gharravi, Anneh Mohammad</creatorcontrib><creatorcontrib>Orazizadeh, Mahmoud</creatorcontrib><creatorcontrib>Hashemitabar, Mahmoud</creatorcontrib><title>Fluid-induced low shear stress improves cartilage like tissue fabrication by encapsulating chondrocytes</title><title>Cell and tissue banking</title><addtitle>Cell Tissue Bank</addtitle><addtitle>Cell Tissue Bank</addtitle><description>In the recent years, there has been considerable development in the regenerative medicine, which aims to repair, regenerate, and improve injured articular cartilage. The aim of the present study was to investigate the effect of flow-induced shear stress in perfusion bioreactor on alginate encapsulating chondrocytes. The shear stress imposed on the cells in the culture chamber of bioreactor was predicted with computational fluid dynamic. Bovine nasal chondrocytes were isolated and expanded to obtain a pellet. The cell pellet was resuspends in alginate solution, transferred to the culture chamber, and dynamically cultured under direct perfusion. At the end of culture, tissue constructs were examined histologically and by immunohistochemistry. The results of computational fluid dynamic modeling revealed that maximum wall shear stress was 4.820 × 10
−3
Pascal. Macroscopic views of the alginate/chondrocyte beads suggested that it possessed constant shape but were flexible. Under inverted microscope, round shape of chondrocyte observed. Cell distribution was homogeneous throughout the scaffold. Tissue construct subjected to shear showed morphological features, which are characteristic for natural cartilage. Immunohistochemistry results revealed immunopositivity for type II collagens in tissue constructs samples. Flow induced shear stress in the perfusion bioreactor and chnondrocyte encapsulation provide environment to support cell growth, and tissue regeneration and improve cartilage like tissue fabrication.</description><subject>Alginates - pharmacology</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cartilage, Articular - drug effects</subject><subject>Cartilage, Articular - physiology</subject><subject>Cattle</subject><subject>Cell Biology</subject><subject>Cells, Cultured</subject><subject>Cells, Immobilized - cytology</subject><subject>Chondrocytes - cytology</subject><subject>Chondrocytes - drug effects</subject><subject>Chondrocytes - metabolism</subject><subject>Fluid dynamics</subject><subject>Glucuronic Acid - pharmacology</subject><subject>Hexuronic Acids - pharmacology</subject><subject>Hydrodynamics</subject><subject>Immunohistochemistry</subject><subject>Life Sciences</subject><subject>Membrane reactors</subject><subject>Microspheres</subject><subject>Original Paper</subject><subject>Rheology</subject><subject>Shear stress</subject><subject>Stress, Mechanical</subject><subject>Tissue engineering</subject><subject>Tissue Engineering - methods</subject><subject>Tissue Scaffolds - chemistry</subject><subject>Transplant Surgery</subject><issn>1389-9333</issn><issn>1573-6814</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkU1r3DAQhkVpaJJtf0AvRdBLLmr1LesYQtIWAr00ZyHL440Sr73R2An772N3NyUUCj1JjJ55R9JDyEfBvwjO3VcU3FjBuDDMG-mZfENOhHGK2Urot_NeVZ55pdQxOUW841xyJ9U7ciytNNp4eULWV92UG5b7ZkrQ0G54ongLsVAcCyDSvNmW4RGQpljG3MU10C7fAx0z4gS0jXXJKY556Gm9o9CnuMWpmwv9mqbboW_KkHYj4Hty1MYO4cNhXZGbq8tfF9_Z9c9vPy7Or1nSzoxMahUr03hlZeN5NDKlVrTCNdKLVPEauJbRWDVXKqO989Z5rtpK19ZooZ1akbN97nzthwlwDJuMCbou9jBMGIRz1mprufwfVFZKLv-3Ip__Qu-GqfTzQ35TXDunlkCxp1IZEAu0YVvyJpZdEDwswsJeWJiFhUVYWHo-HZKnegPNn44XQzMg9wDOR_0ayqvR_0x9BnuOn7o</recordid><startdate>20160301</startdate><enddate>20160301</enddate><creator>Gharravi, Anneh Mohammad</creator><creator>Orazizadeh, Mahmoud</creator><creator>Hashemitabar, Mahmoud</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20160301</creationdate><title>Fluid-induced low shear stress improves cartilage like tissue fabrication by encapsulating chondrocytes</title><author>Gharravi, Anneh Mohammad ; Orazizadeh, Mahmoud ; Hashemitabar, Mahmoud</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-243a85d9362d90a52ccf1f17d291c80be042a56317d85497967903f84b6541473</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Alginates - pharmacology</topic><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cartilage, Articular - drug effects</topic><topic>Cartilage, Articular - physiology</topic><topic>Cattle</topic><topic>Cell Biology</topic><topic>Cells, Cultured</topic><topic>Cells, Immobilized - cytology</topic><topic>Chondrocytes - cytology</topic><topic>Chondrocytes - drug effects</topic><topic>Chondrocytes - metabolism</topic><topic>Fluid dynamics</topic><topic>Glucuronic Acid - pharmacology</topic><topic>Hexuronic Acids - pharmacology</topic><topic>Hydrodynamics</topic><topic>Immunohistochemistry</topic><topic>Life Sciences</topic><topic>Membrane reactors</topic><topic>Microspheres</topic><topic>Original Paper</topic><topic>Rheology</topic><topic>Shear stress</topic><topic>Stress, Mechanical</topic><topic>Tissue engineering</topic><topic>Tissue Engineering - methods</topic><topic>Tissue Scaffolds - chemistry</topic><topic>Transplant Surgery</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gharravi, Anneh Mohammad</creatorcontrib><creatorcontrib>Orazizadeh, Mahmoud</creatorcontrib><creatorcontrib>Hashemitabar, Mahmoud</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Virology and AIDS Abstracts</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Cell and tissue banking</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gharravi, Anneh Mohammad</au><au>Orazizadeh, Mahmoud</au><au>Hashemitabar, Mahmoud</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fluid-induced low shear stress improves cartilage like tissue fabrication by encapsulating chondrocytes</atitle><jtitle>Cell and tissue banking</jtitle><stitle>Cell Tissue Bank</stitle><addtitle>Cell Tissue Bank</addtitle><date>2016-03-01</date><risdate>2016</risdate><volume>17</volume><issue>1</issue><spage>117</spage><epage>122</epage><pages>117-122</pages><issn>1389-9333</issn><eissn>1573-6814</eissn><abstract>In the recent years, there has been considerable development in the regenerative medicine, which aims to repair, regenerate, and improve injured articular cartilage. The aim of the present study was to investigate the effect of flow-induced shear stress in perfusion bioreactor on alginate encapsulating chondrocytes. The shear stress imposed on the cells in the culture chamber of bioreactor was predicted with computational fluid dynamic. Bovine nasal chondrocytes were isolated and expanded to obtain a pellet. The cell pellet was resuspends in alginate solution, transferred to the culture chamber, and dynamically cultured under direct perfusion. At the end of culture, tissue constructs were examined histologically and by immunohistochemistry. The results of computational fluid dynamic modeling revealed that maximum wall shear stress was 4.820 × 10
−3
Pascal. Macroscopic views of the alginate/chondrocyte beads suggested that it possessed constant shape but were flexible. Under inverted microscope, round shape of chondrocyte observed. Cell distribution was homogeneous throughout the scaffold. Tissue construct subjected to shear showed morphological features, which are characteristic for natural cartilage. Immunohistochemistry results revealed immunopositivity for type II collagens in tissue constructs samples. Flow induced shear stress in the perfusion bioreactor and chnondrocyte encapsulation provide environment to support cell growth, and tissue regeneration and improve cartilage like tissue fabrication.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>26254592</pmid><doi>10.1007/s10561-015-9529-2</doi><tpages>6</tpages></addata></record> |
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| subjects | Alginates - pharmacology Animals Biomedical and Life Sciences Biomedicine Cartilage, Articular - drug effects Cartilage, Articular - physiology Cattle Cell Biology Cells, Cultured Cells, Immobilized - cytology Chondrocytes - cytology Chondrocytes - drug effects Chondrocytes - metabolism Fluid dynamics Glucuronic Acid - pharmacology Hexuronic Acids - pharmacology Hydrodynamics Immunohistochemistry Life Sciences Membrane reactors Microspheres Original Paper Rheology Shear stress Stress, Mechanical Tissue engineering Tissue Engineering - methods Tissue Scaffolds - chemistry Transplant Surgery |
| title | Fluid-induced low shear stress improves cartilage like tissue fabrication by encapsulating chondrocytes |
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