Target-Induced and Equipment-Free DNA Amplification with a Simple Paper Device
We report on a paper device capable of carrying out target‐induced rolling circle amplification (RCA) to produce massive DNA amplicons that can be easily visualized. Interestingly, we observed that RCA was more proficient on paper than in solution, which we attribute to a significantly higher locali...
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| Veröffentlicht in: | Angewandte Chemie International Edition 2016-02, Vol.55 (8), p.2709-2713 |
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| container_title | Angewandte Chemie International Edition |
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| creator | Liu, Meng Hui, Christy Y. Zhang, Qiang Gu, Jimmy Kannan, Balamurali Jahanshahi-Anbuhi, Sana Filipe, Carlos D. M. Brennan, John D. Li, Yingfu |
| description | We report on a paper device capable of carrying out target‐induced rolling circle amplification (RCA) to produce massive DNA amplicons that can be easily visualized. Interestingly, we observed that RCA was more proficient on paper than in solution, which we attribute to a significantly higher localized concentration of immobilized DNA. Furthermore, we have successfully engineered a fully functional paper device for sensitive DNA or microRNA detection via printing of all RCA‐enabling molecules within a polymeric sugar film formed from pullulan, which was integrated with the paper device. This encapsulation not only stabilizes the entrapped reagents at room temperature but also enables colorimetric bioassays with minimal steps.
DNA amplification is better on paper: A highly functional, “all‐in‐one” DNA amplification based paper device allows for visual detection of DNA and microRNA with minimal human intervention, making this method particularly suitable for bioassays used in resource‐poor settings. |
| doi_str_mv | 10.1002/anie.201509389 |
| format | Article |
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DNA amplification is better on paper: A highly functional, “all‐in‐one” DNA amplification based paper device allows for visual detection of DNA and microRNA with minimal human intervention, making this method particularly suitable for bioassays used in resource‐poor settings.</description><edition>International ed. in English</edition><identifier>ISSN: 1433-7851</identifier><identifier>EISSN: 1521-3773</identifier><identifier>DOI: 10.1002/anie.201509389</identifier><identifier>PMID: 26748431</identifier><identifier>CODEN: ACIEAY</identifier><language>eng</language><publisher>Germany: Blackwell Publishing Ltd</publisher><subject>Amplification ; Bioassays ; biosensor ; Colorimetry ; Deoxyribonucleic acid ; DNA ; DNA - chemistry ; Encapsulation ; Fluorescence ; miRNA ; point of care diagnostics ; Polymer films ; Printing ; Pullulan ; Reagents ; Ribonucleic acid ; RNA ; rolling circle amplification ; Sugar ; Temperature effects</subject><ispartof>Angewandte Chemie International Edition, 2016-02, Vol.55 (8), p.2709-2713</ispartof><rights>2016 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><rights>2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6499-9e1410ede13125e49fa48617277f0e59ade868d0695d6e13e812c07643524c8e3</citedby><cites>FETCH-LOGICAL-c6499-9e1410ede13125e49fa48617277f0e59ade868d0695d6e13e812c07643524c8e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fanie.201509389$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fanie.201509389$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26748431$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Meng</creatorcontrib><creatorcontrib>Hui, Christy Y.</creatorcontrib><creatorcontrib>Zhang, Qiang</creatorcontrib><creatorcontrib>Gu, Jimmy</creatorcontrib><creatorcontrib>Kannan, Balamurali</creatorcontrib><creatorcontrib>Jahanshahi-Anbuhi, Sana</creatorcontrib><creatorcontrib>Filipe, Carlos D. M.</creatorcontrib><creatorcontrib>Brennan, John D.</creatorcontrib><creatorcontrib>Li, Yingfu</creatorcontrib><title>Target-Induced and Equipment-Free DNA Amplification with a Simple Paper Device</title><title>Angewandte Chemie International Edition</title><addtitle>Angew. Chem. Int. Ed</addtitle><description>We report on a paper device capable of carrying out target‐induced rolling circle amplification (RCA) to produce massive DNA amplicons that can be easily visualized. Interestingly, we observed that RCA was more proficient on paper than in solution, which we attribute to a significantly higher localized concentration of immobilized DNA. Furthermore, we have successfully engineered a fully functional paper device for sensitive DNA or microRNA detection via printing of all RCA‐enabling molecules within a polymeric sugar film formed from pullulan, which was integrated with the paper device. This encapsulation not only stabilizes the entrapped reagents at room temperature but also enables colorimetric bioassays with minimal steps.
DNA amplification is better on paper: A highly functional, “all‐in‐one” DNA amplification based paper device allows for visual detection of DNA and microRNA with minimal human intervention, making this method particularly suitable for bioassays used in resource‐poor settings.</description><subject>Amplification</subject><subject>Bioassays</subject><subject>biosensor</subject><subject>Colorimetry</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA - chemistry</subject><subject>Encapsulation</subject><subject>Fluorescence</subject><subject>miRNA</subject><subject>point of care diagnostics</subject><subject>Polymer films</subject><subject>Printing</subject><subject>Pullulan</subject><subject>Reagents</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>rolling circle amplification</subject><subject>Sugar</subject><subject>Temperature effects</subject><issn>1433-7851</issn><issn>1521-3773</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUtvEzEURi0Eog_YskSW2LCZ4PdjGdokjVSlSC3q0jIzd8BlXrVnaPvv6yglQiwoK19Z5ztXVx9C7yiZUULYJ98FmDFCJbHc2BfokEpGC641f5lnwXmhjaQH6Cilm8wbQ9RrdMCUFkZweog2Vz5-h7FYd9VUQoV9V-HF7RSGFrqxWEYAfLqZ43k7NKEOpR9D3-G7MP7AHl-G_Av4ix8g4lP4FUp4g17Vvknw9uk9Rl-Xi6uTs-L8YrU-mZ8XpRLWFhaooAQqoJwyCcLWXhhFNdO6JiCtr8AoUxFlZaUyBIaykmgluGSiNMCP0cedd4j97QRpdG1IJTSN76CfkqNaKyVk3vIfqJKW5d1b9MNf6E0_xS4f4qglhilCLPsnlV0sXyFVpmY7qox9ShFqN8TQ-vjgKHHb6ty2OrevLgfeP2mnby1Ue_x3VxmwO-AuNPDwjM7NN-vFn_Jilw1phPt91sefTmmupbverNzl56VYLfm1W_FHPDyv4A</recordid><startdate>20160218</startdate><enddate>20160218</enddate><creator>Liu, Meng</creator><creator>Hui, Christy Y.</creator><creator>Zhang, Qiang</creator><creator>Gu, Jimmy</creator><creator>Kannan, Balamurali</creator><creator>Jahanshahi-Anbuhi, Sana</creator><creator>Filipe, Carlos D. M.</creator><creator>Brennan, John D.</creator><creator>Li, Yingfu</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>20160218</creationdate><title>Target-Induced and Equipment-Free DNA Amplification with a Simple Paper Device</title><author>Liu, Meng ; Hui, Christy Y. ; Zhang, Qiang ; Gu, Jimmy ; Kannan, Balamurali ; Jahanshahi-Anbuhi, Sana ; Filipe, Carlos D. M. ; Brennan, John D. ; Li, Yingfu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6499-9e1410ede13125e49fa48617277f0e59ade868d0695d6e13e812c07643524c8e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Amplification</topic><topic>Bioassays</topic><topic>biosensor</topic><topic>Colorimetry</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA - chemistry</topic><topic>Encapsulation</topic><topic>Fluorescence</topic><topic>miRNA</topic><topic>point of care diagnostics</topic><topic>Polymer films</topic><topic>Printing</topic><topic>Pullulan</topic><topic>Reagents</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>rolling circle amplification</topic><topic>Sugar</topic><topic>Temperature effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Meng</creatorcontrib><creatorcontrib>Hui, Christy Y.</creatorcontrib><creatorcontrib>Zhang, Qiang</creatorcontrib><creatorcontrib>Gu, Jimmy</creatorcontrib><creatorcontrib>Kannan, Balamurali</creatorcontrib><creatorcontrib>Jahanshahi-Anbuhi, Sana</creatorcontrib><creatorcontrib>Filipe, Carlos D. M.</creatorcontrib><creatorcontrib>Brennan, John D.</creatorcontrib><creatorcontrib>Li, Yingfu</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Angewandte Chemie International Edition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Meng</au><au>Hui, Christy Y.</au><au>Zhang, Qiang</au><au>Gu, Jimmy</au><au>Kannan, Balamurali</au><au>Jahanshahi-Anbuhi, Sana</au><au>Filipe, Carlos D. M.</au><au>Brennan, John D.</au><au>Li, Yingfu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Target-Induced and Equipment-Free DNA Amplification with a Simple Paper Device</atitle><jtitle>Angewandte Chemie International Edition</jtitle><addtitle>Angew. Chem. Int. Ed</addtitle><date>2016-02-18</date><risdate>2016</risdate><volume>55</volume><issue>8</issue><spage>2709</spage><epage>2713</epage><pages>2709-2713</pages><issn>1433-7851</issn><eissn>1521-3773</eissn><coden>ACIEAY</coden><abstract>We report on a paper device capable of carrying out target‐induced rolling circle amplification (RCA) to produce massive DNA amplicons that can be easily visualized. Interestingly, we observed that RCA was more proficient on paper than in solution, which we attribute to a significantly higher localized concentration of immobilized DNA. Furthermore, we have successfully engineered a fully functional paper device for sensitive DNA or microRNA detection via printing of all RCA‐enabling molecules within a polymeric sugar film formed from pullulan, which was integrated with the paper device. This encapsulation not only stabilizes the entrapped reagents at room temperature but also enables colorimetric bioassays with minimal steps.
DNA amplification is better on paper: A highly functional, “all‐in‐one” DNA amplification based paper device allows for visual detection of DNA and microRNA with minimal human intervention, making this method particularly suitable for bioassays used in resource‐poor settings.</abstract><cop>Germany</cop><pub>Blackwell Publishing Ltd</pub><pmid>26748431</pmid><doi>10.1002/anie.201509389</doi><tpages>5</tpages><edition>International ed. in English</edition></addata></record> |
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| subjects | Amplification Bioassays biosensor Colorimetry Deoxyribonucleic acid DNA DNA - chemistry Encapsulation Fluorescence miRNA point of care diagnostics Polymer films Printing Pullulan Reagents Ribonucleic acid RNA rolling circle amplification Sugar Temperature effects |
| title | Target-Induced and Equipment-Free DNA Amplification with a Simple Paper Device |
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