Antimicrobial Activity of Saturated Fatty Acids and Fatty Amines against Methicillin-Resistant Staphylococcus aureus
The objective of this study was to investigate the antimicrobial activities of saturated fatty acids and fatty amines against methicillin-resistant Staphylococcus aureus (MRSA). The antimicrobial activity of saturated fatty acids and fatty amines was determined by oxygen meters with multi-channels a...
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| Veröffentlicht in: | Biological & Pharmaceutical Bulletin 2004, Vol.27(9), pp.1321-1326 |
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| creator | Kitahara, Takashi Koyama, Nao Matsuda, Junichi Aoyama, Yuko Hirakata, Yoichi Kamihira, Shimeru Kohno, Shigeru Nakashima, Mikiro Sasaki, Hitoshi |
| description | The objective of this study was to investigate the antimicrobial activities of saturated fatty acids and fatty amines against methicillin-resistant Staphylococcus aureus (MRSA). The antimicrobial activity of saturated fatty acids and fatty amines was determined by oxygen meters with multi-channels and disposable oxygen electrode sensors (DOX-96). Lauric acid, the most effective among the saturated fatty acids, showed antimicrobial activity at 400 μg/ml against methicillin-susceptible Staphylococcus aureus (MSSA) and MRSA. The minimal inhibitory concentration (MIC) of fatty amines depended on each hydrophobic chain length. The MIC of myristylamine was 1.56 μg/ml; most effective of the fatty amines. In time-kill curves, lauric acid and myristylamine produced a bactericidal effect and a bacteriostatic effect at 4-fold the MIC, respectively. The antimicrobial activities of lauric acid and myristylamine were decreased by human plasma. Cytotoxicity of 3 saturated fatty acids and 3 fatty amines was examined in cultured endothelial cells. Although cytotoxicity of fatty amines was severer than that of saturated fatty acids, myristylamine showed the highest value of apparent therapeutic index among them. DOX-96 was useful for screening antimicrobial substances, especially in the case of insoluble substances. We found that myristylamine showed anti-MRSA activity comparable to that of vancomycin and teicoplanin. |
| doi_str_mv | 10.1248/bpb.27.1321 |
| format | Article |
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The antimicrobial activity of saturated fatty acids and fatty amines was determined by oxygen meters with multi-channels and disposable oxygen electrode sensors (DOX-96). Lauric acid, the most effective among the saturated fatty acids, showed antimicrobial activity at 400 μg/ml against methicillin-susceptible Staphylococcus aureus (MSSA) and MRSA. The minimal inhibitory concentration (MIC) of fatty amines depended on each hydrophobic chain length. The MIC of myristylamine was 1.56 μg/ml; most effective of the fatty amines. In time-kill curves, lauric acid and myristylamine produced a bactericidal effect and a bacteriostatic effect at 4-fold the MIC, respectively. The antimicrobial activities of lauric acid and myristylamine were decreased by human plasma. Cytotoxicity of 3 saturated fatty acids and 3 fatty amines was examined in cultured endothelial cells. Although cytotoxicity of fatty amines was severer than that of saturated fatty acids, myristylamine showed the highest value of apparent therapeutic index among them. DOX-96 was useful for screening antimicrobial substances, especially in the case of insoluble substances. We found that myristylamine showed anti-MRSA activity comparable to that of vancomycin and teicoplanin.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.27.1321</identifier><identifier>PMID: 15340213</identifier><language>eng</language><publisher>Japan: The Pharmaceutical Society of Japan</publisher><subject>Amines - pharmacology ; Amines - toxicity ; Anti-Bacterial Agents - pharmacology ; Anti-Bacterial Agents - toxicity ; antimicrobial activity ; Cell Line ; Cell Survival - drug effects ; fatty acid ; Fatty Acids - pharmacology ; Fatty Acids - toxicity ; fatty amine ; Humans ; Methicillin Resistance ; methicillin-resistant Staphylococcus aureus (MRSA) ; Microbial Sensitivity Tests ; oxygen meter ; Staphylococcus aureus ; Staphylococcus aureus - drug effects</subject><ispartof>Biological and Pharmaceutical Bulletin, 2004, Vol.27(9), pp.1321-1326</ispartof><rights>2004 The Pharmaceutical Society of Japan</rights><rights>Copyright Japan Science and Technology Agency 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c764t-eb812bdc1045b9cef70cb8d4d2c1b938357cec64fbd608b60eae532ced1ce8f3</citedby><cites>FETCH-LOGICAL-c764t-eb812bdc1045b9cef70cb8d4d2c1b938357cec64fbd608b60eae532ced1ce8f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1881,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15340213$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kitahara, Takashi</creatorcontrib><creatorcontrib>Koyama, Nao</creatorcontrib><creatorcontrib>Matsuda, Junichi</creatorcontrib><creatorcontrib>Aoyama, Yuko</creatorcontrib><creatorcontrib>Hirakata, Yoichi</creatorcontrib><creatorcontrib>Kamihira, Shimeru</creatorcontrib><creatorcontrib>Kohno, Shigeru</creatorcontrib><creatorcontrib>Nakashima, Mikiro</creatorcontrib><creatorcontrib>Sasaki, Hitoshi</creatorcontrib><creatorcontrib>Nagasaki University Hospital of Medicine and Dentistry</creatorcontrib><creatorcontrib>Department of Hospital Pharmacy</creatorcontrib><creatorcontrib>Second Department of Internal Medicine</creatorcontrib><creatorcontrib>Department of Laboratory Medicine</creatorcontrib><title>Antimicrobial Activity of Saturated Fatty Acids and Fatty Amines against Methicillin-Resistant Staphylococcus aureus</title><title>Biological & Pharmaceutical Bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>The objective of this study was to investigate the antimicrobial activities of saturated fatty acids and fatty amines against methicillin-resistant Staphylococcus aureus (MRSA). The antimicrobial activity of saturated fatty acids and fatty amines was determined by oxygen meters with multi-channels and disposable oxygen electrode sensors (DOX-96). Lauric acid, the most effective among the saturated fatty acids, showed antimicrobial activity at 400 μg/ml against methicillin-susceptible Staphylococcus aureus (MSSA) and MRSA. The minimal inhibitory concentration (MIC) of fatty amines depended on each hydrophobic chain length. The MIC of myristylamine was 1.56 μg/ml; most effective of the fatty amines. In time-kill curves, lauric acid and myristylamine produced a bactericidal effect and a bacteriostatic effect at 4-fold the MIC, respectively. The antimicrobial activities of lauric acid and myristylamine were decreased by human plasma. Cytotoxicity of 3 saturated fatty acids and 3 fatty amines was examined in cultured endothelial cells. Although cytotoxicity of fatty amines was severer than that of saturated fatty acids, myristylamine showed the highest value of apparent therapeutic index among them. DOX-96 was useful for screening antimicrobial substances, especially in the case of insoluble substances. We found that myristylamine showed anti-MRSA activity comparable to that of vancomycin and teicoplanin.</description><subject>Amines - pharmacology</subject><subject>Amines - toxicity</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Anti-Bacterial Agents - toxicity</subject><subject>antimicrobial activity</subject><subject>Cell Line</subject><subject>Cell Survival - drug effects</subject><subject>fatty acid</subject><subject>Fatty Acids - pharmacology</subject><subject>Fatty Acids - toxicity</subject><subject>fatty amine</subject><subject>Humans</subject><subject>Methicillin Resistance</subject><subject>methicillin-resistant Staphylococcus aureus (MRSA)</subject><subject>Microbial Sensitivity Tests</subject><subject>oxygen meter</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - drug effects</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkU1r3DAQhkVpaTZpT70XQ6GX4K2-bMm3LqFJCymFJnchjcdZLba1teTC_vtq400CvcygmUfvDO8Q8oHRNeNSf3F7t-ZqzQRnr8iKCanKirPqNVnRhumyZpU-I-cx7iilinLxlpyxSkjKmViRtBmTHzxMwXnbFxtI_q9PhyJ0xZ1N82QTtsW1Tbm0Ad_Gwo7P78GPmAsP1o8xFT8xbT34vvdj-Rujj8mOqbhLdr899AECwJzhecI5viNvOttHfH_KF-T--tv91ffy9tfNj6vNbQmqlqlEpxl3LTAqK9cAdoqC061sOTDXCC0qBQi17FxbU-1qihYrwQFbBqg7cUE-L7L7KfyZMSYz-AjY93bEMEfDlKoZ1TqDn_4Dd2GexryaYVI2QtRSyUxdLlQ2K8YJO7Of_GCng2HUHC9h8iUMV-Z4iUx_PGnObsD2hT1Zn4GbBchdD7YPY3YOXyZDVM6HPhhOqTSUckWbnNij_DHk3dWxmpW-Lkq77PkDPo-yU_LQ49NazRIevz-1YGsng6P4B6xVs7g</recordid><startdate>20040901</startdate><enddate>20040901</enddate><creator>Kitahara, Takashi</creator><creator>Koyama, Nao</creator><creator>Matsuda, Junichi</creator><creator>Aoyama, Yuko</creator><creator>Hirakata, Yoichi</creator><creator>Kamihira, Shimeru</creator><creator>Kohno, Shigeru</creator><creator>Nakashima, Mikiro</creator><creator>Sasaki, Hitoshi</creator><general>The Pharmaceutical Society of Japan</general><general>Pharmaceutical Society of Japan</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7T7</scope><scope>C1K</scope></search><sort><creationdate>20040901</creationdate><title>Antimicrobial Activity of Saturated Fatty Acids and Fatty Amines against Methicillin-Resistant Staphylococcus aureus</title><author>Kitahara, Takashi ; Koyama, Nao ; Matsuda, Junichi ; Aoyama, Yuko ; Hirakata, Yoichi ; Kamihira, Shimeru ; Kohno, Shigeru ; Nakashima, Mikiro ; Sasaki, Hitoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c764t-eb812bdc1045b9cef70cb8d4d2c1b938357cec64fbd608b60eae532ced1ce8f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Amines - pharmacology</topic><topic>Amines - toxicity</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Anti-Bacterial Agents - toxicity</topic><topic>antimicrobial activity</topic><topic>Cell Line</topic><topic>Cell Survival - drug effects</topic><topic>fatty acid</topic><topic>Fatty Acids - pharmacology</topic><topic>Fatty Acids - toxicity</topic><topic>fatty amine</topic><topic>Humans</topic><topic>Methicillin Resistance</topic><topic>methicillin-resistant Staphylococcus aureus (MRSA)</topic><topic>Microbial Sensitivity Tests</topic><topic>oxygen meter</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kitahara, Takashi</creatorcontrib><creatorcontrib>Koyama, Nao</creatorcontrib><creatorcontrib>Matsuda, Junichi</creatorcontrib><creatorcontrib>Aoyama, Yuko</creatorcontrib><creatorcontrib>Hirakata, Yoichi</creatorcontrib><creatorcontrib>Kamihira, Shimeru</creatorcontrib><creatorcontrib>Kohno, Shigeru</creatorcontrib><creatorcontrib>Nakashima, Mikiro</creatorcontrib><creatorcontrib>Sasaki, Hitoshi</creatorcontrib><creatorcontrib>Nagasaki University Hospital of Medicine and Dentistry</creatorcontrib><creatorcontrib>Department of Hospital Pharmacy</creatorcontrib><creatorcontrib>Second Department of Internal Medicine</creatorcontrib><creatorcontrib>Department of Laboratory Medicine</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Biological & Pharmaceutical Bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kitahara, Takashi</au><au>Koyama, Nao</au><au>Matsuda, Junichi</au><au>Aoyama, Yuko</au><au>Hirakata, Yoichi</au><au>Kamihira, Shimeru</au><au>Kohno, Shigeru</au><au>Nakashima, Mikiro</au><au>Sasaki, Hitoshi</au><aucorp>Nagasaki University Hospital of Medicine and Dentistry</aucorp><aucorp>Department of Hospital Pharmacy</aucorp><aucorp>Second Department of Internal Medicine</aucorp><aucorp>Department of Laboratory Medicine</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antimicrobial Activity of Saturated Fatty Acids and Fatty Amines against Methicillin-Resistant Staphylococcus aureus</atitle><jtitle>Biological & Pharmaceutical Bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>2004-09-01</date><risdate>2004</risdate><volume>27</volume><issue>9</issue><spage>1321</spage><epage>1326</epage><pages>1321-1326</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>The objective of this study was to investigate the antimicrobial activities of saturated fatty acids and fatty amines against methicillin-resistant Staphylococcus aureus (MRSA). The antimicrobial activity of saturated fatty acids and fatty amines was determined by oxygen meters with multi-channels and disposable oxygen electrode sensors (DOX-96). Lauric acid, the most effective among the saturated fatty acids, showed antimicrobial activity at 400 μg/ml against methicillin-susceptible Staphylococcus aureus (MSSA) and MRSA. The minimal inhibitory concentration (MIC) of fatty amines depended on each hydrophobic chain length. The MIC of myristylamine was 1.56 μg/ml; most effective of the fatty amines. In time-kill curves, lauric acid and myristylamine produced a bactericidal effect and a bacteriostatic effect at 4-fold the MIC, respectively. The antimicrobial activities of lauric acid and myristylamine were decreased by human plasma. Cytotoxicity of 3 saturated fatty acids and 3 fatty amines was examined in cultured endothelial cells. Although cytotoxicity of fatty amines was severer than that of saturated fatty acids, myristylamine showed the highest value of apparent therapeutic index among them. DOX-96 was useful for screening antimicrobial substances, especially in the case of insoluble substances. We found that myristylamine showed anti-MRSA activity comparable to that of vancomycin and teicoplanin.</abstract><cop>Japan</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>15340213</pmid><doi>10.1248/bpb.27.1321</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amines - pharmacology Amines - toxicity Anti-Bacterial Agents - pharmacology Anti-Bacterial Agents - toxicity antimicrobial activity Cell Line Cell Survival - drug effects fatty acid Fatty Acids - pharmacology Fatty Acids - toxicity fatty amine Humans Methicillin Resistance methicillin-resistant Staphylococcus aureus (MRSA) Microbial Sensitivity Tests oxygen meter Staphylococcus aureus Staphylococcus aureus - drug effects |
| title | Antimicrobial Activity of Saturated Fatty Acids and Fatty Amines against Methicillin-Resistant Staphylococcus aureus |
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