Distribution of Garlic virus A in different garlic production regions of Argentina [Allium sativum L.]
Comparative tests using inmunosorbent electron microscopy plus decoration and ELISA with antibodies against Garlic virus A (GarV-A) and Garlic virus C (GarV-C) coat proteins showed that they specifically recognize the respective viruses. On this basis, the GarV-A antiserum was used in DAS-ELISA test...
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| Veröffentlicht in: | Journal of plant pathology 2000-03, Vol.82 (1), p.17-21 |
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| creator | Lunello, P Helguera, M Nome, S.F Concil, V.C. (Instituto Nacional de Tecnologia Agropecuaria (INTA), Cordoba (Argentina). Instituto de Fitopatologia y Fisiologia Vegetal) Bravo-Almonacid, F Kobayashi, K Mentaberry, A. (Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Buenos Aires (Argentina)) |
| description | Comparative tests using inmunosorbent electron microscopy plus decoration and ELISA with antibodies against Garlic virus A (GarV-A) and Garlic virus C (GarV-C) coat proteins showed that they specifically recognize the respective viruses. On this basis, the GarV-A antiserum was used in DAS-ELISA tests to study the distribution of GarV-A in different regions of Argentina. A total of 2,655 samples, representing 115 garlic fields, were analyzed. GarV-A was detected in 64%, 51%, 43% and 35% of samples collected in San Juan, Mendoza, Cordoba and Buenos Aires Provinces, respectively. Positive samples were not found in plants from Santa Cruz Province. Distribution among different garlic cultivars showed that this virus was present in 33%, 46%, 53%, 70% and 43% of plants belonging to cvs Colorado, Chino, Violeta Frances, Blanco and Rosado Paraguayo, respectively. Negative results were obtained in samples of cvs Castano-INTA and Violeta Santacruceno
[Test comparativi compiuti utilizzando la microscopia elettronica immuno assorbente piu' decorazione e l'ELISA con anticorpi contro le proteine capsidiche del virus A dell'aglio (GarV-A) e del virus C dell'aglio (GarV-C) hanno dimostrato che questi sono in grado di identificare i rispettivi virus. Su questa base, l'antisiero di GarV-A e' stato utilizzato in test DAS-ELISA per studiare la distribuzione di GarV-A in regioni diverse dell'Argentina. E' stato analizzato un totale di 2.655 campioni, rappresentativi di 115 appezzamenti coltivati con aglio. GarV-A e' stato identificato nel 64%, 51%, 43% e 35% dei campioni raccolti nelle province di San Juan, Mendoza, Cordoba e Buenos Aires, rispettivamente. Non sono stati ritrovati campioni positivi in piante provenienti dalla provincia di Santa Cruz. L'esame della distribuzione entro le diverse cultivar di aglio ha consentito di evidenziare che questo virus era presente nel 33%, 46%, 53%, 70% e 43% delle piante appartenenti alle cv Colorado, Chino, Violeta Frances, Blanco e Rosado Paraguayo, rispettivamente. Sono stati ottenuti risultati negativi nei campioni delle cv Castano-INTA e Violeta Santacruceno] |
| format | Article |
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[Test comparativi compiuti utilizzando la microscopia elettronica immuno assorbente piu' decorazione e l'ELISA con anticorpi contro le proteine capsidiche del virus A dell'aglio (GarV-A) e del virus C dell'aglio (GarV-C) hanno dimostrato che questi sono in grado di identificare i rispettivi virus. Su questa base, l'antisiero di GarV-A e' stato utilizzato in test DAS-ELISA per studiare la distribuzione di GarV-A in regioni diverse dell'Argentina. E' stato analizzato un totale di 2.655 campioni, rappresentativi di 115 appezzamenti coltivati con aglio. GarV-A e' stato identificato nel 64%, 51%, 43% e 35% dei campioni raccolti nelle province di San Juan, Mendoza, Cordoba e Buenos Aires, rispettivamente. Non sono stati ritrovati campioni positivi in piante provenienti dalla provincia di Santa Cruz. L'esame della distribuzione entro le diverse cultivar di aglio ha consentito di evidenziare che questo virus era presente nel 33%, 46%, 53%, 70% e 43% delle piante appartenenti alle cv Colorado, Chino, Violeta Frances, Blanco e Rosado Paraguayo, rispettivamente. Sono stati ottenuti risultati negativi nei campioni delle cv Castano-INTA e Violeta Santacruceno]</description><identifier>ISSN: 1125-4653</identifier><identifier>EISSN: 2239-7264</identifier><language>eng</language><publisher>An International Journal of the Italian Phytopathological Society</publisher><subject>AGLIO ; AIL ; AJO ; ALLIUM SATIVUM ; Antibodies ; Antiserum ; ARGENTINA ; ARGENTINE ; Capsid proteins ; Carlavirus ; DISEASE RESISTANCE ; DISEASE SURVEYS ; ELISA ; ENCUESTAS SANITARIAS ; ENQUETE PATHOLOGIQUE ; Enzyme linked immunosorbent assay ; GARLIC ; Garlic virus a ; INDAGINI SULLE MALATTIE ; MICROSCOPIA ; MICROSCOPIE ; MICROSCOPY ; Phytopathology ; PLANT VIRUSES ; Plants ; RESISTANCE AUX MALADIES ; RESISTENCIA A LA ENFERMEDAD ; RESISTENZA ALLE MALATTIE ; TEST ELISA ; VARIEDADES ; VARIETA ; VARIETE ; VARIETIES ; Virology ; VIRUS DE LAS PLANTAS ; VIRUS DELLE PIANTE ; VIRUS DES VEGETAUX ; Viruses</subject><ispartof>Journal of plant pathology, 2000-03, Vol.82 (1), p.17-21</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/41997975$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/41997975$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,58017,58250</link.rule.ids></links><search><creatorcontrib>Lunello, P</creatorcontrib><creatorcontrib>Helguera, M</creatorcontrib><creatorcontrib>Nome, S.F</creatorcontrib><creatorcontrib>Concil, V.C. (Instituto Nacional de Tecnologia Agropecuaria (INTA), Cordoba (Argentina). Instituto de Fitopatologia y Fisiologia Vegetal)</creatorcontrib><creatorcontrib>Bravo-Almonacid, F</creatorcontrib><creatorcontrib>Kobayashi, K</creatorcontrib><creatorcontrib>Mentaberry, A. (Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Buenos Aires (Argentina))</creatorcontrib><title>Distribution of Garlic virus A in different garlic production regions of Argentina [Allium sativum L.]</title><title>Journal of plant pathology</title><description>Comparative tests using inmunosorbent electron microscopy plus decoration and ELISA with antibodies against Garlic virus A (GarV-A) and Garlic virus C (GarV-C) coat proteins showed that they specifically recognize the respective viruses. On this basis, the GarV-A antiserum was used in DAS-ELISA tests to study the distribution of GarV-A in different regions of Argentina. A total of 2,655 samples, representing 115 garlic fields, were analyzed. GarV-A was detected in 64%, 51%, 43% and 35% of samples collected in San Juan, Mendoza, Cordoba and Buenos Aires Provinces, respectively. Positive samples were not found in plants from Santa Cruz Province. Distribution among different garlic cultivars showed that this virus was present in 33%, 46%, 53%, 70% and 43% of plants belonging to cvs Colorado, Chino, Violeta Frances, Blanco and Rosado Paraguayo, respectively. Negative results were obtained in samples of cvs Castano-INTA and Violeta Santacruceno
[Test comparativi compiuti utilizzando la microscopia elettronica immuno assorbente piu' decorazione e l'ELISA con anticorpi contro le proteine capsidiche del virus A dell'aglio (GarV-A) e del virus C dell'aglio (GarV-C) hanno dimostrato che questi sono in grado di identificare i rispettivi virus. Su questa base, l'antisiero di GarV-A e' stato utilizzato in test DAS-ELISA per studiare la distribuzione di GarV-A in regioni diverse dell'Argentina. E' stato analizzato un totale di 2.655 campioni, rappresentativi di 115 appezzamenti coltivati con aglio. GarV-A e' stato identificato nel 64%, 51%, 43% e 35% dei campioni raccolti nelle province di San Juan, Mendoza, Cordoba e Buenos Aires, rispettivamente. Non sono stati ritrovati campioni positivi in piante provenienti dalla provincia di Santa Cruz. L'esame della distribuzione entro le diverse cultivar di aglio ha consentito di evidenziare che questo virus era presente nel 33%, 46%, 53%, 70% e 43% delle piante appartenenti alle cv Colorado, Chino, Violeta Frances, Blanco e Rosado Paraguayo, rispettivamente. Sono stati ottenuti risultati negativi nei campioni delle cv Castano-INTA e Violeta Santacruceno]</description><subject>AGLIO</subject><subject>AIL</subject><subject>AJO</subject><subject>ALLIUM SATIVUM</subject><subject>Antibodies</subject><subject>Antiserum</subject><subject>ARGENTINA</subject><subject>ARGENTINE</subject><subject>Capsid proteins</subject><subject>Carlavirus</subject><subject>DISEASE RESISTANCE</subject><subject>DISEASE SURVEYS</subject><subject>ELISA</subject><subject>ENCUESTAS SANITARIAS</subject><subject>ENQUETE PATHOLOGIQUE</subject><subject>Enzyme linked immunosorbent assay</subject><subject>GARLIC</subject><subject>Garlic virus a</subject><subject>INDAGINI SULLE MALATTIE</subject><subject>MICROSCOPIA</subject><subject>MICROSCOPIE</subject><subject>MICROSCOPY</subject><subject>Phytopathology</subject><subject>PLANT VIRUSES</subject><subject>Plants</subject><subject>RESISTANCE AUX MALADIES</subject><subject>RESISTENCIA A LA ENFERMEDAD</subject><subject>RESISTENZA ALLE MALATTIE</subject><subject>TEST ELISA</subject><subject>VARIEDADES</subject><subject>VARIETA</subject><subject>VARIETE</subject><subject>VARIETIES</subject><subject>Virology</subject><subject>VIRUS DE LAS PLANTAS</subject><subject>VIRUS DELLE PIANTE</subject><subject>VIRUS DES VEGETAUX</subject><subject>Viruses</subject><issn>1125-4653</issn><issn>2239-7264</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNpFjktLxDAYRYMoOI7-BCErd5U8mqRZlvE1MOBmXImUNMlXMnTaMUkH_PdWR3B1Fvfcyz1DC8a4LhST5TlaUMpEUUrBL9FVSjtCSqGIXCB4CCnH0E45jAMeAT-b2AeLjyFOCdc4DNgFAB_9kHF3yg5xdJP9LUTfzUg_xTp2sxMGg9_rvg_THieTw3Hm5v7jGl2A6ZO_-eMSvT09blcvxeb1eb2qNwXQUuTCKeeIJaQVYMHyVglHNFipHfVVK5iqdAuGgObUOOmlqEglQWppgFWVkHyJ7k6788fPyafc7EOyvu_N4McpNVSpstJSz-LtSdylPMbmEMPexK-mpForrcR_DmZsTBdDatZbRggjkghO-TdjzWhM</recordid><startdate>20000301</startdate><enddate>20000301</enddate><creator>Lunello, P</creator><creator>Helguera, M</creator><creator>Nome, S.F</creator><creator>Concil, V.C. (Instituto Nacional de Tecnologia Agropecuaria (INTA), Cordoba (Argentina). Instituto de Fitopatologia y Fisiologia Vegetal)</creator><creator>Bravo-Almonacid, F</creator><creator>Kobayashi, K</creator><creator>Mentaberry, A. (Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Buenos Aires (Argentina))</creator><general>An International Journal of the Italian Phytopathological Society</general><scope>FBQ</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>20000301</creationdate><title>Distribution of Garlic virus A in different garlic production regions of Argentina [Allium sativum L.]</title><author>Lunello, P ; Helguera, M ; Nome, S.F ; Concil, V.C. (Instituto Nacional de Tecnologia Agropecuaria (INTA), Cordoba (Argentina). Instituto de Fitopatologia y Fisiologia Vegetal) ; Bravo-Almonacid, F ; Kobayashi, K ; Mentaberry, A. (Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Buenos Aires (Argentina))</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f145t-d7dd0c00b5fcfc3b75d09fc69d1e8b52789bfa0f931ad6e658086f696af288563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>AGLIO</topic><topic>AIL</topic><topic>AJO</topic><topic>ALLIUM SATIVUM</topic><topic>Antibodies</topic><topic>Antiserum</topic><topic>ARGENTINA</topic><topic>ARGENTINE</topic><topic>Capsid proteins</topic><topic>Carlavirus</topic><topic>DISEASE RESISTANCE</topic><topic>DISEASE SURVEYS</topic><topic>ELISA</topic><topic>ENCUESTAS SANITARIAS</topic><topic>ENQUETE PATHOLOGIQUE</topic><topic>Enzyme linked immunosorbent assay</topic><topic>GARLIC</topic><topic>Garlic virus a</topic><topic>INDAGINI SULLE MALATTIE</topic><topic>MICROSCOPIA</topic><topic>MICROSCOPIE</topic><topic>MICROSCOPY</topic><topic>Phytopathology</topic><topic>PLANT VIRUSES</topic><topic>Plants</topic><topic>RESISTANCE AUX MALADIES</topic><topic>RESISTENCIA A LA ENFERMEDAD</topic><topic>RESISTENZA ALLE MALATTIE</topic><topic>TEST ELISA</topic><topic>VARIEDADES</topic><topic>VARIETA</topic><topic>VARIETE</topic><topic>VARIETIES</topic><topic>Virology</topic><topic>VIRUS DE LAS PLANTAS</topic><topic>VIRUS DELLE PIANTE</topic><topic>VIRUS DES VEGETAUX</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lunello, P</creatorcontrib><creatorcontrib>Helguera, M</creatorcontrib><creatorcontrib>Nome, S.F</creatorcontrib><creatorcontrib>Concil, V.C. (Instituto Nacional de Tecnologia Agropecuaria (INTA), Cordoba (Argentina). Instituto de Fitopatologia y Fisiologia Vegetal)</creatorcontrib><creatorcontrib>Bravo-Almonacid, F</creatorcontrib><creatorcontrib>Kobayashi, K</creatorcontrib><creatorcontrib>Mentaberry, A. (Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Buenos Aires (Argentina))</creatorcontrib><collection>AGRIS</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Journal of plant pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lunello, P</au><au>Helguera, M</au><au>Nome, S.F</au><au>Concil, V.C. (Instituto Nacional de Tecnologia Agropecuaria (INTA), Cordoba (Argentina). Instituto de Fitopatologia y Fisiologia Vegetal)</au><au>Bravo-Almonacid, F</au><au>Kobayashi, K</au><au>Mentaberry, A. (Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Buenos Aires (Argentina))</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distribution of Garlic virus A in different garlic production regions of Argentina [Allium sativum L.]</atitle><jtitle>Journal of plant pathology</jtitle><date>2000-03-01</date><risdate>2000</risdate><volume>82</volume><issue>1</issue><spage>17</spage><epage>21</epage><pages>17-21</pages><issn>1125-4653</issn><eissn>2239-7264</eissn><abstract>Comparative tests using inmunosorbent electron microscopy plus decoration and ELISA with antibodies against Garlic virus A (GarV-A) and Garlic virus C (GarV-C) coat proteins showed that they specifically recognize the respective viruses. On this basis, the GarV-A antiserum was used in DAS-ELISA tests to study the distribution of GarV-A in different regions of Argentina. A total of 2,655 samples, representing 115 garlic fields, were analyzed. GarV-A was detected in 64%, 51%, 43% and 35% of samples collected in San Juan, Mendoza, Cordoba and Buenos Aires Provinces, respectively. Positive samples were not found in plants from Santa Cruz Province. Distribution among different garlic cultivars showed that this virus was present in 33%, 46%, 53%, 70% and 43% of plants belonging to cvs Colorado, Chino, Violeta Frances, Blanco and Rosado Paraguayo, respectively. Negative results were obtained in samples of cvs Castano-INTA and Violeta Santacruceno
[Test comparativi compiuti utilizzando la microscopia elettronica immuno assorbente piu' decorazione e l'ELISA con anticorpi contro le proteine capsidiche del virus A dell'aglio (GarV-A) e del virus C dell'aglio (GarV-C) hanno dimostrato che questi sono in grado di identificare i rispettivi virus. Su questa base, l'antisiero di GarV-A e' stato utilizzato in test DAS-ELISA per studiare la distribuzione di GarV-A in regioni diverse dell'Argentina. E' stato analizzato un totale di 2.655 campioni, rappresentativi di 115 appezzamenti coltivati con aglio. GarV-A e' stato identificato nel 64%, 51%, 43% e 35% dei campioni raccolti nelle province di San Juan, Mendoza, Cordoba e Buenos Aires, rispettivamente. Non sono stati ritrovati campioni positivi in piante provenienti dalla provincia di Santa Cruz. L'esame della distribuzione entro le diverse cultivar di aglio ha consentito di evidenziare che questo virus era presente nel 33%, 46%, 53%, 70% e 43% delle piante appartenenti alle cv Colorado, Chino, Violeta Frances, Blanco e Rosado Paraguayo, rispettivamente. Sono stati ottenuti risultati negativi nei campioni delle cv Castano-INTA e Violeta Santacruceno]</abstract><pub>An International Journal of the Italian Phytopathological Society</pub><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1125-4653 |
| ispartof | Journal of plant pathology, 2000-03, Vol.82 (1), p.17-21 |
| issn | 1125-4653 2239-7264 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17748969 |
| source | Jstor Complete Legacy; Alma/SFX Local Collection |
| subjects | AGLIO AIL AJO ALLIUM SATIVUM Antibodies Antiserum ARGENTINA ARGENTINE Capsid proteins Carlavirus DISEASE RESISTANCE DISEASE SURVEYS ELISA ENCUESTAS SANITARIAS ENQUETE PATHOLOGIQUE Enzyme linked immunosorbent assay GARLIC Garlic virus a INDAGINI SULLE MALATTIE MICROSCOPIA MICROSCOPIE MICROSCOPY Phytopathology PLANT VIRUSES Plants RESISTANCE AUX MALADIES RESISTENCIA A LA ENFERMEDAD RESISTENZA ALLE MALATTIE TEST ELISA VARIEDADES VARIETA VARIETE VARIETIES Virology VIRUS DE LAS PLANTAS VIRUS DELLE PIANTE VIRUS DES VEGETAUX Viruses |
| title | Distribution of Garlic virus A in different garlic production regions of Argentina [Allium sativum L.] |
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