CHARACTERIZATION OF PASTEURELLA MULTOCIDA ASSOCIATED WITH PNEUMONIA IN BIGHORN SHEEP
Pasteurella multocida is a highly diverse group of bacteria recognized as important pathogens. Although P. multocida is not ordinarily associated with disease in Rocky Mountain bighorn sheep (Ovis canadensis canadensis), numerous isolates were cultured in high numbers from free-ranging bighorn sheep...
Gespeichert in:
| Veröffentlicht in: | Journal of wildlife diseases 2003-07, Vol.39 (3), p.536-544 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 544 |
|---|---|
| container_issue | 3 |
| container_start_page | 536 |
| container_title | Journal of wildlife diseases |
| container_volume | 39 |
| creator | Weiser, Glen C. DeLong, Walter J. Paz, Julia L. Shafii, Bahman Price, William J. Ward, Alton C. S. |
| description | Pasteurella multocida is a highly diverse group of bacteria recognized as important pathogens. Although P. multocida is not ordinarily associated with disease in Rocky Mountain bighorn sheep (Ovis canadensis canadensis), numerous isolates were cultured in high numbers from free-ranging bighorn sheep in the Hells Canyon area of Idaho, Washington, and Oregon (USA) during the winter of 1995–96. Animals captured in Hells Canyon and held in captivity, and their offspring, also harbored P. multocida. Biochemical utilization tests on 90 isolates identified three subspecies: P. multocida multocida a (n=54); P. multocida multocida b (n=13); and P. multocida gallicida (n=15); and a non-speciated biotype, U6 (n=8). Genomic DNA digestion with restriction endonuclease Hha I separated the isolates into 62 unique restriction fragment length polymorphism profiles. Capsular type A was predominant (72% of isolates). Only one isolate type, which may have been transmitted from a feral goat, was capsular type D, possessed the structural gene, toxA, for dermonecrotoxin detected by polymerase chain reaction, and produced toxin as determined by monoclonal antibody immunoblot. In conclusion, bighorn sheep in this study carried diverse types of generally non-toxigenic P. multocida associated with epizootic pneumonia. |
| doi_str_mv | 10.7589/0090-3558-39.3.536 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_17745440</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17745440</sourcerecordid><originalsourceid>FETCH-LOGICAL-b469t-ac504df64bcbb9d8751d7c013e5e1d1fc34a1561bcc8db90f2ca1ec392b927353</originalsourceid><addsrcrecordid>eNqNkEFv0zAYhi0EYmXwBziAL3BLZ8d2XB9Nl62RsqRqEiFxsWzH2YLSZcSrKv49rlIBx50-f9LzvrYfAD5itORsJa4QEigijK0iIpZkyUjyCiywoCQiHKHXYPEXuADvvP-JUMzC8hZcYMoSHmO6APV6I3dyXae77Iess7KA5Q3cyqpOm12a5xLeNXldrrNrCWVVhYOs02v4Pas3cFukzV1ZZBJmBfyW3W7KXQGrTZpu34M3nR68-3Cel6C5ScNNUV7eZmuZR4Ym4jnSliHadgk11hjRrjjDLbcIE8ccbnFnCdWYJdhYu2qNQF1sNXaWiNiImBNGLsHXufdpGn8dnH9W-95bNwz60Y0HrzDnlFGKAhjPoJ1G7yfXqaep3-vpt8JInVyqkyp1UqWIUEQFlyH06dx-MHvX_ouc5QXgyww89PcPx35yyu_1MAQ8Vsfj8b-izzPX6VHp-6n3qqni8E-EEcaCi0CgmTD9OD66l7zuD57gjIo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17745440</pqid></control><display><type>article</type><title>CHARACTERIZATION OF PASTEURELLA MULTOCIDA ASSOCIATED WITH PNEUMONIA IN BIGHORN SHEEP</title><source>MEDLINE</source><source>BioOne Open Access Titles</source><source>Allen Press Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Weiser, Glen C. ; DeLong, Walter J. ; Paz, Julia L. ; Shafii, Bahman ; Price, William J. ; Ward, Alton C. S.</creator><creatorcontrib>Weiser, Glen C. ; DeLong, Walter J. ; Paz, Julia L. ; Shafii, Bahman ; Price, William J. ; Ward, Alton C. S.</creatorcontrib><description>Pasteurella multocida is a highly diverse group of bacteria recognized as important pathogens. Although P. multocida is not ordinarily associated with disease in Rocky Mountain bighorn sheep (Ovis canadensis canadensis), numerous isolates were cultured in high numbers from free-ranging bighorn sheep in the Hells Canyon area of Idaho, Washington, and Oregon (USA) during the winter of 1995–96. Animals captured in Hells Canyon and held in captivity, and their offspring, also harbored P. multocida. Biochemical utilization tests on 90 isolates identified three subspecies: P. multocida multocida a (n=54); P. multocida multocida b (n=13); and P. multocida gallicida (n=15); and a non-speciated biotype, U6 (n=8). Genomic DNA digestion with restriction endonuclease Hha I separated the isolates into 62 unique restriction fragment length polymorphism profiles. Capsular type A was predominant (72% of isolates). Only one isolate type, which may have been transmitted from a feral goat, was capsular type D, possessed the structural gene, toxA, for dermonecrotoxin detected by polymerase chain reaction, and produced toxin as determined by monoclonal antibody immunoblot. In conclusion, bighorn sheep in this study carried diverse types of generally non-toxigenic P. multocida associated with epizootic pneumonia.</description><identifier>ISSN: 0090-3558</identifier><identifier>EISSN: 1943-3700</identifier><identifier>DOI: 10.7589/0090-3558-39.3.536</identifier><identifier>PMID: 14567214</identifier><language>eng</language><publisher>United States: Wildlife Disease Association</publisher><subject>Animals ; Animals, Wild ; BACTERIOLOGY AND MYCOLOGY ; Capsular type ; dermonecrotoxin ; disease transmission ; DNA, Bacterial - analysis ; Female ; Idaho - epidemiology ; Male ; Oregon - epidemiology ; Ovis canadensis ; Ovis canadensis canadensis ; Pasteurella Infections - epidemiology ; Pasteurella Infections - microbiology ; Pasteurella Infections - veterinary ; Pasteurella multocida ; Pasteurella multocida - classification ; Pasteurella multocida - isolation & purification ; Pasteurella multocida - pathogenicity ; pneumonia epizootic ; Pneumonia, Bacterial - epidemiology ; Pneumonia, Bacterial - microbiology ; Pneumonia, Bacterial - veterinary ; polymerase chain reaction ; Polymerase Chain Reaction - veterinary ; Polymorphism, Restriction Fragment Length ; restriction fragment length polymorphism ; Sheep ; Sheep Diseases - epidemiology ; Sheep Diseases - microbiology ; virulence ; Washington - epidemiology</subject><ispartof>Journal of wildlife diseases, 2003-07, Vol.39 (3), p.536-544</ispartof><rights>Wildlife Disease Association 2003</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b469t-ac504df64bcbb9d8751d7c013e5e1d1fc34a1561bcc8db90f2ca1ec392b927353</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://bioone.org/doi/pdf/10.7589/0090-3558-39.3.536$$EPDF$$P50$$Gbioone$$H</linktopdf><link.rule.ids>109,315,781,785,27929,27930,52724</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14567214$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Weiser, Glen C.</creatorcontrib><creatorcontrib>DeLong, Walter J.</creatorcontrib><creatorcontrib>Paz, Julia L.</creatorcontrib><creatorcontrib>Shafii, Bahman</creatorcontrib><creatorcontrib>Price, William J.</creatorcontrib><creatorcontrib>Ward, Alton C. S.</creatorcontrib><title>CHARACTERIZATION OF PASTEURELLA MULTOCIDA ASSOCIATED WITH PNEUMONIA IN BIGHORN SHEEP</title><title>Journal of wildlife diseases</title><addtitle>J Wildl Dis</addtitle><description>Pasteurella multocida is a highly diverse group of bacteria recognized as important pathogens. Although P. multocida is not ordinarily associated with disease in Rocky Mountain bighorn sheep (Ovis canadensis canadensis), numerous isolates were cultured in high numbers from free-ranging bighorn sheep in the Hells Canyon area of Idaho, Washington, and Oregon (USA) during the winter of 1995–96. Animals captured in Hells Canyon and held in captivity, and their offspring, also harbored P. multocida. Biochemical utilization tests on 90 isolates identified three subspecies: P. multocida multocida a (n=54); P. multocida multocida b (n=13); and P. multocida gallicida (n=15); and a non-speciated biotype, U6 (n=8). Genomic DNA digestion with restriction endonuclease Hha I separated the isolates into 62 unique restriction fragment length polymorphism profiles. Capsular type A was predominant (72% of isolates). Only one isolate type, which may have been transmitted from a feral goat, was capsular type D, possessed the structural gene, toxA, for dermonecrotoxin detected by polymerase chain reaction, and produced toxin as determined by monoclonal antibody immunoblot. In conclusion, bighorn sheep in this study carried diverse types of generally non-toxigenic P. multocida associated with epizootic pneumonia.</description><subject>Animals</subject><subject>Animals, Wild</subject><subject>BACTERIOLOGY AND MYCOLOGY</subject><subject>Capsular type</subject><subject>dermonecrotoxin</subject><subject>disease transmission</subject><subject>DNA, Bacterial - analysis</subject><subject>Female</subject><subject>Idaho - epidemiology</subject><subject>Male</subject><subject>Oregon - epidemiology</subject><subject>Ovis canadensis</subject><subject>Ovis canadensis canadensis</subject><subject>Pasteurella Infections - epidemiology</subject><subject>Pasteurella Infections - microbiology</subject><subject>Pasteurella Infections - veterinary</subject><subject>Pasteurella multocida</subject><subject>Pasteurella multocida - classification</subject><subject>Pasteurella multocida - isolation & purification</subject><subject>Pasteurella multocida - pathogenicity</subject><subject>pneumonia epizootic</subject><subject>Pneumonia, Bacterial - epidemiology</subject><subject>Pneumonia, Bacterial - microbiology</subject><subject>Pneumonia, Bacterial - veterinary</subject><subject>polymerase chain reaction</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>restriction fragment length polymorphism</subject><subject>Sheep</subject><subject>Sheep Diseases - epidemiology</subject><subject>Sheep Diseases - microbiology</subject><subject>virulence</subject><subject>Washington - epidemiology</subject><issn>0090-3558</issn><issn>1943-3700</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEFv0zAYhi0EYmXwBziAL3BLZ8d2XB9Nl62RsqRqEiFxsWzH2YLSZcSrKv49rlIBx50-f9LzvrYfAD5itORsJa4QEigijK0iIpZkyUjyCiywoCQiHKHXYPEXuADvvP-JUMzC8hZcYMoSHmO6APV6I3dyXae77Iess7KA5Q3cyqpOm12a5xLeNXldrrNrCWVVhYOs02v4Pas3cFukzV1ZZBJmBfyW3W7KXQGrTZpu34M3nR68-3Cel6C5ScNNUV7eZmuZR4Ym4jnSliHadgk11hjRrjjDLbcIE8ccbnFnCdWYJdhYu2qNQF1sNXaWiNiImBNGLsHXufdpGn8dnH9W-95bNwz60Y0HrzDnlFGKAhjPoJ1G7yfXqaep3-vpt8JInVyqkyp1UqWIUEQFlyH06dx-MHvX_ouc5QXgyww89PcPx35yyu_1MAQ8Vsfj8b-izzPX6VHp-6n3qqni8E-EEcaCi0CgmTD9OD66l7zuD57gjIo</recordid><startdate>20030701</startdate><enddate>20030701</enddate><creator>Weiser, Glen C.</creator><creator>DeLong, Walter J.</creator><creator>Paz, Julia L.</creator><creator>Shafii, Bahman</creator><creator>Price, William J.</creator><creator>Ward, Alton C. S.</creator><general>Wildlife Disease Association</general><general>Wildlife Dis Assoc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>20030701</creationdate><title>CHARACTERIZATION OF PASTEURELLA MULTOCIDA ASSOCIATED WITH PNEUMONIA IN BIGHORN SHEEP</title><author>Weiser, Glen C. ; DeLong, Walter J. ; Paz, Julia L. ; Shafii, Bahman ; Price, William J. ; Ward, Alton C. S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b469t-ac504df64bcbb9d8751d7c013e5e1d1fc34a1561bcc8db90f2ca1ec392b927353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Animals, Wild</topic><topic>BACTERIOLOGY AND MYCOLOGY</topic><topic>Capsular type</topic><topic>dermonecrotoxin</topic><topic>disease transmission</topic><topic>DNA, Bacterial - analysis</topic><topic>Female</topic><topic>Idaho - epidemiology</topic><topic>Male</topic><topic>Oregon - epidemiology</topic><topic>Ovis canadensis</topic><topic>Ovis canadensis canadensis</topic><topic>Pasteurella Infections - epidemiology</topic><topic>Pasteurella Infections - microbiology</topic><topic>Pasteurella Infections - veterinary</topic><topic>Pasteurella multocida</topic><topic>Pasteurella multocida - classification</topic><topic>Pasteurella multocida - isolation & purification</topic><topic>Pasteurella multocida - pathogenicity</topic><topic>pneumonia epizootic</topic><topic>Pneumonia, Bacterial - epidemiology</topic><topic>Pneumonia, Bacterial - microbiology</topic><topic>Pneumonia, Bacterial - veterinary</topic><topic>polymerase chain reaction</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>restriction fragment length polymorphism</topic><topic>Sheep</topic><topic>Sheep Diseases - epidemiology</topic><topic>Sheep Diseases - microbiology</topic><topic>virulence</topic><topic>Washington - epidemiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Weiser, Glen C.</creatorcontrib><creatorcontrib>DeLong, Walter J.</creatorcontrib><creatorcontrib>Paz, Julia L.</creatorcontrib><creatorcontrib>Shafii, Bahman</creatorcontrib><creatorcontrib>Price, William J.</creatorcontrib><creatorcontrib>Ward, Alton C. S.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Journal of wildlife diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Weiser, Glen C.</au><au>DeLong, Walter J.</au><au>Paz, Julia L.</au><au>Shafii, Bahman</au><au>Price, William J.</au><au>Ward, Alton C. S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CHARACTERIZATION OF PASTEURELLA MULTOCIDA ASSOCIATED WITH PNEUMONIA IN BIGHORN SHEEP</atitle><jtitle>Journal of wildlife diseases</jtitle><addtitle>J Wildl Dis</addtitle><date>2003-07-01</date><risdate>2003</risdate><volume>39</volume><issue>3</issue><spage>536</spage><epage>544</epage><pages>536-544</pages><issn>0090-3558</issn><eissn>1943-3700</eissn><abstract>Pasteurella multocida is a highly diverse group of bacteria recognized as important pathogens. Although P. multocida is not ordinarily associated with disease in Rocky Mountain bighorn sheep (Ovis canadensis canadensis), numerous isolates were cultured in high numbers from free-ranging bighorn sheep in the Hells Canyon area of Idaho, Washington, and Oregon (USA) during the winter of 1995–96. Animals captured in Hells Canyon and held in captivity, and their offspring, also harbored P. multocida. Biochemical utilization tests on 90 isolates identified three subspecies: P. multocida multocida a (n=54); P. multocida multocida b (n=13); and P. multocida gallicida (n=15); and a non-speciated biotype, U6 (n=8). Genomic DNA digestion with restriction endonuclease Hha I separated the isolates into 62 unique restriction fragment length polymorphism profiles. Capsular type A was predominant (72% of isolates). Only one isolate type, which may have been transmitted from a feral goat, was capsular type D, possessed the structural gene, toxA, for dermonecrotoxin detected by polymerase chain reaction, and produced toxin as determined by monoclonal antibody immunoblot. In conclusion, bighorn sheep in this study carried diverse types of generally non-toxigenic P. multocida associated with epizootic pneumonia.</abstract><cop>United States</cop><pub>Wildlife Disease Association</pub><pmid>14567214</pmid><doi>10.7589/0090-3558-39.3.536</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0090-3558 |
| ispartof | Journal of wildlife diseases, 2003-07, Vol.39 (3), p.536-544 |
| issn | 0090-3558 1943-3700 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17745440 |
| source | MEDLINE; BioOne Open Access Titles; Allen Press Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Animals, Wild BACTERIOLOGY AND MYCOLOGY Capsular type dermonecrotoxin disease transmission DNA, Bacterial - analysis Female Idaho - epidemiology Male Oregon - epidemiology Ovis canadensis Ovis canadensis canadensis Pasteurella Infections - epidemiology Pasteurella Infections - microbiology Pasteurella Infections - veterinary Pasteurella multocida Pasteurella multocida - classification Pasteurella multocida - isolation & purification Pasteurella multocida - pathogenicity pneumonia epizootic Pneumonia, Bacterial - epidemiology Pneumonia, Bacterial - microbiology Pneumonia, Bacterial - veterinary polymerase chain reaction Polymerase Chain Reaction - veterinary Polymorphism, Restriction Fragment Length restriction fragment length polymorphism Sheep Sheep Diseases - epidemiology Sheep Diseases - microbiology virulence Washington - epidemiology |
| title | CHARACTERIZATION OF PASTEURELLA MULTOCIDA ASSOCIATED WITH PNEUMONIA IN BIGHORN SHEEP |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-13T10%3A07%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=CHARACTERIZATION%20OF%20PASTEURELLA%20MULTOCIDA%20ASSOCIATED%20WITH%20PNEUMONIA%20IN%20BIGHORN%20SHEEP&rft.jtitle=Journal%20of%20wildlife%20diseases&rft.au=Weiser,%20Glen%20C.&rft.date=2003-07-01&rft.volume=39&rft.issue=3&rft.spage=536&rft.epage=544&rft.pages=536-544&rft.issn=0090-3558&rft.eissn=1943-3700&rft_id=info:doi/10.7589/0090-3558-39.3.536&rft_dat=%3Cproquest_cross%3E17745440%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17745440&rft_id=info:pmid/14567214&rfr_iscdi=true |