Phosphatidic acid induces EHD3-containing membrane tubulation and is required for receptor recycling
EHD3 is localized on the tubular structures of early endosomes, and it regulates their trafficking pathway. However, the regulatory mechanism of EHD3-containing tubular structures remains poorly understood. An in vitro liposome co-sedimentation assay revealed that EHD3 interacted with phosphatidic a...
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| Veröffentlicht in: | Experimental cell research 2016-03, Vol.342 (1), p.1-10 |
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| creator | Henmi, Yuji Oe, Natsuko Kono, Nozomu Taguchi, Tomohiko Takei, Kohji Tanabe, Kenji |
| description | EHD3 is localized on the tubular structures of early endosomes, and it regulates their trafficking pathway. However, the regulatory mechanism of EHD3-containing tubular structures remains poorly understood. An in vitro liposome co-sedimentation assay revealed that EHD3 interacted with phosphatidic acid through its helical domain and this interaction induced liposomal tubulations. Additionally, inhibiting phosphatidic acid synthesis with diacylglycerol kinase inhibitor or lysophosphatidic acid acyltransferase inhibitor significantly reduced the number of EHD3-containing tubules and impaired their trafficking from early endosomes. These results suggest that EHD3 and phosphatidic acid cooperatively regulate membrane deformation and trafficking from early endosomes.
•EHD3 binds to phosphatidic acid through its helical domain.•The interaction between EHD3 and phosphatidic acid induced liposomal tabulation.•Inhibition of PA synthesis reduced EHD3-containing tubules and impaired trafficking from early endosomes. |
| doi_str_mv | 10.1016/j.yexcr.2016.02.011 |
| format | Article |
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| subjects | Acids Amino Acid Sequence Animals Carrier Proteins - metabolism Cell Surface Extensions - metabolism Cellular biology EHD Endocytosis Endosomes Endosomes - metabolism HeLa Cells Humans Hydrogen-Ion Concentration Membrane tubulation Membranes Mice Molecular Sequence Data Phophatidic acid Phosphatidic Acids - physiology Protein Binding Protein Structure, Secondary Protein Transport Receptor recycling Transport Vesicles - metabolism |
| title | Phosphatidic acid induces EHD3-containing membrane tubulation and is required for receptor recycling |
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