Detection of aflatoxin-producing molds in Korean fermented foods and grains by multiplex PCR
An assay based on multiplex PCR was applied for the detection of potential aflatoxin-producing molds in Korean fermented foods and grains. Three genes, avfA, omtA, and ver-1, coding for key enzymes in aflatoxin biosynthesis, were used as aflatoxin-detecting target genes in multiplex PCR. DNA extract...
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| Veröffentlicht in: | Journal of food protection 2004-11, Vol.67 (11), p.2622-2626 |
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| creator | Yang, Z.Y Shim, W.B Kim, J.H Park, S.J Kang, S.J Nam, B.S Chung, D.H |
| description | An assay based on multiplex PCR was applied for the detection of potential aflatoxin-producing molds in Korean fermented foods and grains. Three genes, avfA, omtA, and ver-1, coding for key enzymes in aflatoxin biosynthesis, were used as aflatoxin-detecting target genes in multiplex PCR. DNA extracted from Aspergillus flavus, Aspergillus parasiticus, Aspergillus oryzae, Aspergillus niger, Aspergillus terreus, Penicillium expansum, and Fusarium verticillioides was used as PCR template to test specificity of the multiplex PCR assay. Positive results were achieved only with DNA that was extracted from the aflatoxigenic molds A. flavus and A. parasiticus in all three primer pairs. This result was supported by aflatoxin detection with direct competitive enzyme-linked immunosorbent assay (DC-ELISA). The PCR assay required just a few hours, enabling rapid and simultaneous detection of many samples at a low cost. A total of 22 Meju samples, 24 Doenjang samples, and 10 barley samples commercially obtained in Korea were analyzed. The DC-ELISA assay for aflatoxin detection gave negative results for all samples, whereas the PCR-based method gave positive results for 1 of 22 Meju samples and 2 of 10 barley samples. After incubation of the positive samples with malt extract agar, DC-ELISA also gave positive results for aflatoxin detection. All Doenjang samples were negative by multiplex PCR and DC-ELISA assay, suggesting that aflatoxin contamination and the presence of aflatoxin-producing molds in Doenjang are probably low. |
| doi_str_mv | 10.4315/0362-028X-67.11.2622 |
| format | Article |
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Three genes, avfA, omtA, and ver-1, coding for key enzymes in aflatoxin biosynthesis, were used as aflatoxin-detecting target genes in multiplex PCR. DNA extracted from Aspergillus flavus, Aspergillus parasiticus, Aspergillus oryzae, Aspergillus niger, Aspergillus terreus, Penicillium expansum, and Fusarium verticillioides was used as PCR template to test specificity of the multiplex PCR assay. Positive results were achieved only with DNA that was extracted from the aflatoxigenic molds A. flavus and A. parasiticus in all three primer pairs. This result was supported by aflatoxin detection with direct competitive enzyme-linked immunosorbent assay (DC-ELISA). The PCR assay required just a few hours, enabling rapid and simultaneous detection of many samples at a low cost. A total of 22 Meju samples, 24 Doenjang samples, and 10 barley samples commercially obtained in Korea were analyzed. The DC-ELISA assay for aflatoxin detection gave negative results for all samples, whereas the PCR-based method gave positive results for 1 of 22 Meju samples and 2 of 10 barley samples. After incubation of the positive samples with malt extract agar, DC-ELISA also gave positive results for aflatoxin detection. All Doenjang samples were negative by multiplex PCR and DC-ELISA assay, suggesting that aflatoxin contamination and the presence of aflatoxin-producing molds in Doenjang are probably low.</description><identifier>ISSN: 0362-028X</identifier><identifier>EISSN: 1944-9097</identifier><identifier>DOI: 10.4315/0362-028X-67.11.2622</identifier><identifier>PMID: 15553652</identifier><identifier>CODEN: JFPRDR</identifier><language>eng</language><publisher>Des Moines, IA: International Association of Milk, Food and Environmental Sanitarians</publisher><subject>aflatoxins ; Aflatoxins - isolation & purification ; Aspergillus flavus ; Aspergillus niger ; Aspergillus oryzae ; Aspergillus parasiticus ; Aspergillus terreus ; Biological and medical sciences ; Cereal and baking product industries ; DNA Primers ; DNA, Fungal - analysis ; Edible Grain - microbiology ; Enzyme-Linked Immunosorbent Assay ; Fermentation ; fermented foods ; food contamination ; Food Contamination - analysis ; Food industries ; Food Microbiology ; food pathogens ; Fundamental and applied biological sciences. Psychology ; Fusarium verticillioides ; General aspects ; genes ; Methods of analysis, processing and quality control, regulation, standards ; microbial detection ; molds (fungi) ; Penicillium expansum ; polymerase chain reaction ; Polymerase Chain Reaction - methods ; rapid methods ; Sensitivity and Specificity ; serotypes ; small grains ; soybean products ; Time Factors ; toxigenic strains ; traditional foods ; virulence</subject><ispartof>Journal of food protection, 2004-11, Vol.67 (11), p.2622-2626</ispartof><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c485t-99f2d21c5ba4094896405191d75644dec4896f645c775807ea08b0b341a39fe73</citedby><cites>FETCH-LOGICAL-c485t-99f2d21c5ba4094896405191d75644dec4896f645c775807ea08b0b341a39fe73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16254508$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15553652$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Z.Y</creatorcontrib><creatorcontrib>Shim, W.B</creatorcontrib><creatorcontrib>Kim, J.H</creatorcontrib><creatorcontrib>Park, S.J</creatorcontrib><creatorcontrib>Kang, S.J</creatorcontrib><creatorcontrib>Nam, B.S</creatorcontrib><creatorcontrib>Chung, D.H</creatorcontrib><title>Detection of aflatoxin-producing molds in Korean fermented foods and grains by multiplex PCR</title><title>Journal of food protection</title><addtitle>J Food Prot</addtitle><description>An assay based on multiplex PCR was applied for the detection of potential aflatoxin-producing molds in Korean fermented foods and grains. Three genes, avfA, omtA, and ver-1, coding for key enzymes in aflatoxin biosynthesis, were used as aflatoxin-detecting target genes in multiplex PCR. DNA extracted from Aspergillus flavus, Aspergillus parasiticus, Aspergillus oryzae, Aspergillus niger, Aspergillus terreus, Penicillium expansum, and Fusarium verticillioides was used as PCR template to test specificity of the multiplex PCR assay. Positive results were achieved only with DNA that was extracted from the aflatoxigenic molds A. flavus and A. parasiticus in all three primer pairs. This result was supported by aflatoxin detection with direct competitive enzyme-linked immunosorbent assay (DC-ELISA). The PCR assay required just a few hours, enabling rapid and simultaneous detection of many samples at a low cost. A total of 22 Meju samples, 24 Doenjang samples, and 10 barley samples commercially obtained in Korea were analyzed. The DC-ELISA assay for aflatoxin detection gave negative results for all samples, whereas the PCR-based method gave positive results for 1 of 22 Meju samples and 2 of 10 barley samples. After incubation of the positive samples with malt extract agar, DC-ELISA also gave positive results for aflatoxin detection. All Doenjang samples were negative by multiplex PCR and DC-ELISA assay, suggesting that aflatoxin contamination and the presence of aflatoxin-producing molds in Doenjang are probably low.</description><subject>aflatoxins</subject><subject>Aflatoxins - isolation & purification</subject><subject>Aspergillus flavus</subject><subject>Aspergillus niger</subject><subject>Aspergillus oryzae</subject><subject>Aspergillus parasiticus</subject><subject>Aspergillus terreus</subject><subject>Biological and medical sciences</subject><subject>Cereal and baking product industries</subject><subject>DNA Primers</subject><subject>DNA, Fungal - analysis</subject><subject>Edible Grain - microbiology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fermentation</subject><subject>fermented foods</subject><subject>food contamination</subject><subject>Food Contamination - analysis</subject><subject>Food industries</subject><subject>Food Microbiology</subject><subject>food pathogens</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fusarium verticillioides</subject><subject>General aspects</subject><subject>genes</subject><subject>Methods of analysis, processing and quality control, regulation, standards</subject><subject>microbial detection</subject><subject>molds (fungi)</subject><subject>Penicillium expansum</subject><subject>polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>rapid methods</subject><subject>Sensitivity and Specificity</subject><subject>serotypes</subject><subject>small grains</subject><subject>soybean products</subject><subject>Time Factors</subject><subject>toxigenic strains</subject><subject>traditional foods</subject><subject>virulence</subject><issn>0362-028X</issn><issn>1944-9097</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0F1rFDEUgOEgit1W_4FoburdrCffk0tZay0tKGrBCyFkMskSmUnWZAbaf-8Mu9irQPKcQ3gRekNgyxkRH4BJ2gBtfzVSbQnZUknpM7QhmvNGg1bP0eY_OUPntf4BAKqpfInOiBCCSUE36PcnP3k3xZxwDtiGwU75IabmUHI_u5j2eMxDX3FM-DYXbxMOvow-Tb7HIeflxaYe74uNqeLuEY_zMMXD4B_wt933V-hFsEP1r0_nBbr_fPVz96W5-3p9s_t41zjeiqnROtCeEic6y0HzVksOgmjSKyE5771br4LkwiklWlDeQttBxzixTAev2AV6f9y7_Prv7OtkxlidHwabfJ6rIUpxoFQvkB-hK7nW4oM5lDja8mgImLWqWZOZNZmRyhBi1qrL2NvT_rkbff80dMq4gMsTsNXZIRSbXKxPTlLBBbSLe3d0wWZj92Ux9z8oEAagJdEM2D-CsYgi</recordid><startdate>20041101</startdate><enddate>20041101</enddate><creator>Yang, Z.Y</creator><creator>Shim, W.B</creator><creator>Kim, J.H</creator><creator>Park, S.J</creator><creator>Kang, S.J</creator><creator>Nam, B.S</creator><creator>Chung, D.H</creator><general>International Association of Milk, Food and Environmental Sanitarians</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>20041101</creationdate><title>Detection of aflatoxin-producing molds in Korean fermented foods and grains by multiplex PCR</title><author>Yang, Z.Y ; Shim, W.B ; Kim, J.H ; Park, S.J ; Kang, S.J ; Nam, B.S ; Chung, D.H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c485t-99f2d21c5ba4094896405191d75644dec4896f645c775807ea08b0b341a39fe73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>aflatoxins</topic><topic>Aflatoxins - isolation & purification</topic><topic>Aspergillus flavus</topic><topic>Aspergillus niger</topic><topic>Aspergillus oryzae</topic><topic>Aspergillus parasiticus</topic><topic>Aspergillus terreus</topic><topic>Biological and medical sciences</topic><topic>Cereal and baking product industries</topic><topic>DNA Primers</topic><topic>DNA, Fungal - analysis</topic><topic>Edible Grain - microbiology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fermentation</topic><topic>fermented foods</topic><topic>food contamination</topic><topic>Food Contamination - analysis</topic><topic>Food industries</topic><topic>Food Microbiology</topic><topic>food pathogens</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fusarium verticillioides</topic><topic>General aspects</topic><topic>genes</topic><topic>Methods of analysis, processing and quality control, regulation, standards</topic><topic>microbial detection</topic><topic>molds (fungi)</topic><topic>Penicillium expansum</topic><topic>polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>rapid methods</topic><topic>Sensitivity and Specificity</topic><topic>serotypes</topic><topic>small grains</topic><topic>soybean products</topic><topic>Time Factors</topic><topic>toxigenic strains</topic><topic>traditional foods</topic><topic>virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Z.Y</creatorcontrib><creatorcontrib>Shim, W.B</creatorcontrib><creatorcontrib>Kim, J.H</creatorcontrib><creatorcontrib>Park, S.J</creatorcontrib><creatorcontrib>Kang, S.J</creatorcontrib><creatorcontrib>Nam, B.S</creatorcontrib><creatorcontrib>Chung, D.H</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of food protection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Z.Y</au><au>Shim, W.B</au><au>Kim, J.H</au><au>Park, S.J</au><au>Kang, S.J</au><au>Nam, B.S</au><au>Chung, D.H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of aflatoxin-producing molds in Korean fermented foods and grains by multiplex PCR</atitle><jtitle>Journal of food protection</jtitle><addtitle>J Food Prot</addtitle><date>2004-11-01</date><risdate>2004</risdate><volume>67</volume><issue>11</issue><spage>2622</spage><epage>2626</epage><pages>2622-2626</pages><issn>0362-028X</issn><eissn>1944-9097</eissn><coden>JFPRDR</coden><abstract>An assay based on multiplex PCR was applied for the detection of potential aflatoxin-producing molds in Korean fermented foods and grains. Three genes, avfA, omtA, and ver-1, coding for key enzymes in aflatoxin biosynthesis, were used as aflatoxin-detecting target genes in multiplex PCR. DNA extracted from Aspergillus flavus, Aspergillus parasiticus, Aspergillus oryzae, Aspergillus niger, Aspergillus terreus, Penicillium expansum, and Fusarium verticillioides was used as PCR template to test specificity of the multiplex PCR assay. Positive results were achieved only with DNA that was extracted from the aflatoxigenic molds A. flavus and A. parasiticus in all three primer pairs. This result was supported by aflatoxin detection with direct competitive enzyme-linked immunosorbent assay (DC-ELISA). The PCR assay required just a few hours, enabling rapid and simultaneous detection of many samples at a low cost. A total of 22 Meju samples, 24 Doenjang samples, and 10 barley samples commercially obtained in Korea were analyzed. The DC-ELISA assay for aflatoxin detection gave negative results for all samples, whereas the PCR-based method gave positive results for 1 of 22 Meju samples and 2 of 10 barley samples. After incubation of the positive samples with malt extract agar, DC-ELISA also gave positive results for aflatoxin detection. All Doenjang samples were negative by multiplex PCR and DC-ELISA assay, suggesting that aflatoxin contamination and the presence of aflatoxin-producing molds in Doenjang are probably low.</abstract><cop>Des Moines, IA</cop><pub>International Association of Milk, Food and Environmental Sanitarians</pub><pmid>15553652</pmid><doi>10.4315/0362-028X-67.11.2622</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | aflatoxins Aflatoxins - isolation & purification Aspergillus flavus Aspergillus niger Aspergillus oryzae Aspergillus parasiticus Aspergillus terreus Biological and medical sciences Cereal and baking product industries DNA Primers DNA, Fungal - analysis Edible Grain - microbiology Enzyme-Linked Immunosorbent Assay Fermentation fermented foods food contamination Food Contamination - analysis Food industries Food Microbiology food pathogens Fundamental and applied biological sciences. Psychology Fusarium verticillioides General aspects genes Methods of analysis, processing and quality control, regulation, standards microbial detection molds (fungi) Penicillium expansum polymerase chain reaction Polymerase Chain Reaction - methods rapid methods Sensitivity and Specificity serotypes small grains soybean products Time Factors toxigenic strains traditional foods virulence |
| title | Detection of aflatoxin-producing molds in Korean fermented foods and grains by multiplex PCR |
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